Now the only difficult thing about spectrophotometry is how to say it www.gelifesciences.com

Now the only difficult thing
about spectrophotometry
is how to say it
www.gelifesciences.com
1
Contents
Introduction3
Spectrophotometer selection guide
4
High performance analytics
Ultrospec™ 9000
Ultrospec 8000
Ultrospec 7000
Ultrospec 7000, 8000 & 9000 Accessories 2
5
6
7
8
Options for enzyme kinetics
Ultrospec 2100 pro 10
Low volumes
NanoVue™ Plus SimpliNano™ Fixed pathlength
11
12
Compact, convenient, and flexible
GeneQuant™ 1300 13
Basic enzyme kinetics
Novaspec™ Plus
Novaspec III
14
14
Hand-held cell density meter
Ultrospec 10 Cell Density Meter
15
System control software
Datrys System Contol Software
16
Software and qualification packages
Print Via Computer (PVC) software
Summary of software by instrument
18
18
Related products
19
Introduction
UV-Visible spectrophotometers are a ubiquitous tool in
the life sciences because of the wide range of
molecules that can be detected and analyzed using
this technique. Like many other everyday items, it’s
easy to forget how long you have had your
spectrophotometer and you might not think about its
performance or capabilities until you need to replace
it. Questions to consider include:
•What applications are routinely run in your
laboratory? Could this change in the near future?
•What range of sample volumes do you need to
measure? If this varies, look for options for different
cuvette sizes and adaptors
•Do you want to measure multiple samples at the same
time? If so, consider options for multiple cell holders
•What level of sensitivity do you need? For low
concentration samples, a wider bandwidth
(3 to 5 nm) will give more consistent results
than a narrow (1 nm) bandwidth instrument
•How many users will there be and what level of
experience will they have? Preprogrammed methods
and password control of your own methods can be
useful features
•How will data be recorded, output or stored?
This guide is an introduction to the range of
spectrophotometers available from GE Healthcare
Life Sciences.
Whether you are performing simple colorimetric assays
or conducting the most stringent research, there is an
instrument from our range to meet your needs.
Low sample volumes
NanoVue Plus, with dual pathlengths, offers both
convenience and a wide dynamic range for life science
applications for volumes 0.5 to 5.0 µl. SimpliNano fixed
pathlength spectrophotometer offers superb accuracy
for nucleic acid and protein A280 measurements, as low
as 1 µl. The ability to pipette samples directly onto the
sample plate (NanoVue Plus) or port (SimpliNano)
eliminates the need for cuvettes or other sample devices.
Routine applications
GE Healthcare offers a number of spectrophotometers
for widely used applications such as DNA and RNA
quantitation, protein concentration determination, and
cell density measurements. This includes Ultrospec™
10, a battery-operated, portable, cell density meter;
Novaspec III and Plus for protein determination and/or
enzyme kinetics; and GeneQuant 100 and 1300
instruments.
Additional features
For more advanced features, Ultrospec 2100 offers
temperature control and cell changer options, while
the Ultrospec 8000 and 9000 are high performance,
dual-beam instruments, for precision measurements
that are compatible with Pharmacopoeia guidelines.
Data output
Many GE Healthcare spectrophotometers have a
variety of data output capabilities. These include
integrated printer, USB cable to PC, USB stick or
Bluetooth™ wireless options.
3
High-performance dual-beam, 2 nm
bandwidth UV-Visible spectrophotometer.
Wavelength: 190-1100 nm.
Recommended for Life Science
applications, with optional Life Science or
CFR Datrys software upgrades.
Ultrospec 2100
3 nm bandwidth instrument. Wavelength:
190-900nm, with 8-position cell changer
and options for temperature control
NanoVue Plus
2 pathlength sample mechanism
eliminates need for cuvettes, facilitates
low-volume measurements (0.5 to 5 µl) and
gives wide dynamic range across life
science applications
SimpliNano
Fixed pathlength low volume (1 to 5 µl)
measurements for nucleic acids and
Protein A280
GeneQuant 1300 As GeneQuant 100 and with built-in
enzyme kinetics, and CyDye™ applications
plus Bluetooth data output options
GeneQuant 100
5 nm bandwidth instrument with built in
applications for nucleic acids, proteins and
cell density
Novaspec Plus
As Novaspec III and with stored protein
methods, easy to read graphical display,
and temperature control option
Novaspec III
7 nm bandwidth visible
spectrophotometer, 330 to 800 nm
Ultrospec 10
Battery-powered, portable dedicated cell
density meter
= recommended;
4
= suitable;
= not suitable
Pharma method
development
Ultrospec 7000
European Pharmacopeia
compatibility
High-performance dual-beam, 1 nm
bandwidth UV-Visible spectrophotometer.
Wavelength: 190-1100 nm. Recommended
for Pharma, QC and analytical labs. CFR
Datrys software upgrades available.
Life Science applications, with optional Life
Science or CFR Datrys software upgrades.
Fluorescent probe
quantitation, cDNA probes
for microarrays, PCR probes
Ultrospec 8000
Enzyme activity
kinetics at 340 nm
High-performance dual-beam, variable
bandwidth UV-Visible spectrophotometer.
Wavelength: 190-1100nm. Recommended
for Pharma, QC and analytical labs. CFR
Datrys software upgrades available.
Cell culture OD600
Ultrospec 9000
PCR/sequencing/
hybridization primer
quantitation and design
Main features
Color protein quantitation
(Bradford, Biuret, BCA™ &
Lowry)
Model
DNA/RNA & direct (A280)
protein quantitation
Spectrophotometer selection guide
Ultrospec 9000/9000PC UV-Visible spectrophotometer
Dual
beam
High performance method
development
Ultrospec 9000/9000PC is a dual-beam UV‑Visible
spectrophotometer for operation in high‑specification
laboratories. The instrument is European
Pharmacopeia compatible and is also compatible
with CFR part 11 requirements via the optional
Datrys software. Method development is supported
through variable bandwidth capability and custom
calculation facilities.
Key features
•Dual-beam, variable bandwidth optics—high
performance for difficult samples
•Full European Pharmacopeia compatibility
Ultrospec 9000 with PC-control option.
•21 CFR part 11 support through Datrys CFR (optional)
•Custom calculation facility for method development
using Datrys software
•Equation Editor software allowing custom calculations
to be applied to measured data
•Color touchscreen (stand-alone instrument)—
simpler operation
•Software for wavelength scanning, fixed wavelength,
standard curves, kinetics and prestored life science
methods as standard
•Stand-alone version also includes Print Via
Computer (PVC)
•Wide range of accessories and options available
including cell changers, thermostatting, sipper,
built-in printer, Bluetooth and Datrys software
options. Please refer to the “High performance
spectrophotometer accessories” brochure
(29‑0043-70) for additional information
•Multiuser analytical instrument with secure login to
protect methods
•USB memory stick data/method storage and USB PC
connection—export data the way you want
Ordering information
•Stand-alone or PC-controlled versions available
Product
•Both instrument versions are supplied with
Datrys Standard, and can be upgraded to Datrys Life
Science or Datrys CFR
Ultrospec 9000 UV-Visible Spectrophotometer
29-0036-16
Ultrospec 9000PC UV-Visible Spectrophotometer
29-0036-17
Ultrospec 9000 UV-Visible Spectrophotometer
with printer accessory
29-0036-18
Ultrospec 9000 UV-Visible Spectrophotometer
with Bluetooth accessory
29-0036-19
Ultrospec 9000 UV-Visible Spectrophotometer with printer
and Bluetooth accessory
29-0036-20
Code number
5
Ultrospec 8000/8000PC UV-Visible spectrophotometer
Dual
beam
High performance analytics
Ultrospec 8000/8000PC is a dual-beam UV‑Visible
spectrophotometer designed to meet the requirements
of critical analysis and users in Pharmacopeia
research environments. Secure user log in and method
control, plus a 1 nm bandwidth enables European
Pharmacopeia compatability. 21 CFR part 11 support
can be achieved with the optional Datrys CFR software.
Key features
•Dual-beam instrument, with a 1 nm bandwidth
•Full European Pharmacopeia compatibility
•21 CFR part 11 support through Datrys CFR (optional)
•Color touchscreen (stand-alone instrument)—
simpler operation
•Software for wavelength scanning, fixed wavelength,
standard curves, kinetics and prestored life science
methods as standard
•Equation Editor software allowing custom
calculations to be applied to measured data
•Multiuser analytical instrument with secure login to
protect methods
•USB memory stick data/method storage and USB PC
connection—export data the way you want
•Stand-alone or PC-controlled versions available
•Both instrument versions are supplied with Datrys
Standard. Can be upgraded to Datrys Life Science or
Datrys CFR
•Stand-alone version also includes Print Via
Computer (PVC)
Ultrospec 8000 stand-alone instrument.
Ordering information
Product
6
Code number
Ultrospec 8000 UV-Visible Spectrophotometer
29-0036-10
Ultrospec 8000PC UV-Visible Spectrophotometer
29-0036-11
Ultrospec 8000 UV-Visible Spectrophotometer
with printer accessory
29-0036-12
Ultrospec 8000 UV-Visible Spectrophotometer
with Bluetooth accessory
29-0036-13
Ultrospec 8000 UV-Visible Spectrophotometer with printer
and Bluetooth accessory
29-0036-14
•Wide range of accessories and options available
including cell changers, thermostatting, sipper,
built-in printer, Bluetooth and Datrys software
options. Please refer to the “High performance
spectrophotometer accessories” brochure
(29‑0043-70) for additional information
Ultrospec 7000/7000PC UV-Visible spectrophotometer
Dual
beam
High performance multiple applications
Ultrospec 7000/7000PC is a multiple application,
dual-beam, UV‑Visible spectrophotometer developed
to meet the day-to-day needs of the busy laboratory.
Engineered for robust, lower cost operation with
minimal maintenance requirements, yet providing
simple, touchscreen operation, multiple user profiles
and a choice of data export methods. A range of
software upgrade and accessory options are available
to meet your needs in the future.
Ultrospec 7000 with PC-control option.
Key features
•Dual-beam instrument with a 2 nm bandwidth
•High-energy Xenon lamp minimizes annual
running costs
•Color touchscreen (stand-alone instrument)—
simpler operation
•Software for wavelength scanning, fixed wavelength,
standard curves, kinetics and prestored life science
methods as standard
•Equation Editor software allowing custom
calculations to be applied to measured data
Ultrospec 7000 stand-alone instrument.
•Multiuser analytical instrument with secure login
to protect methods
•USB memory stick data/method storage and
USB PC connection—export data the way you want
Ordering information
Product
Code number
•Stand-alone or PC-controlled versions available
Ultrospec 7000 UV-Visible Spectrophotometer
29-0036-05
•PC version is supplied with Datrys Standard
Ultrospec 7000PC UV-Visible Spectrophotometer
29-0036-06
Ultrospec 7000 UV-Visible Spectrophotometer
with printer accessory
29-0036-07
Ultrospec 7000 UV-Visible Spectrophotometer
with Bluetooth accessory
•Stand-alone version includes Print Via Computer
(PVC) only—can be upgraded with Datrys software
options including Life Science and CFR
29-0036-08
Ultrospec 7000 UV-Visible Spectrophotometer with printer
and Bluetooth accessory
29-0036-09
•Wide range of accessories and options available
including cell changers, thermostatting, sipper,
built-in printer, Bluetooth and Datrys software
options. Please refer to the “High performance
spectrophotometer accessories” brochure
(29‑0043-70) for additional information
7
Ultrospec 7000, 8000, and 9000 accessories
Accessories
With a large sample compartment, the Ultrospec instruments can be configured with quick
change accessories including sipper, temperature control accessories, long pathlength,
test tube and film holders.
Film Holder
•Holds films from 1 mm to 10 mm thickness
•Holds films up to 160 mm wide and 70 mm high
•Fits to standard sample compartment baseplate, can
be changed in seconds
•Holder installed in sample position only
Ordering information
Product
Film holder for Ultrospec 7000/8000/9000
Code number
80-7100-13
Automatic 8-Cell Changer
•Fully automatic operation
•Fully controlled by software, parameters stored
within application methods
•Fits to standard sample compartment base plate, can
be changed in seconds
Ordering information
Product
Non Thermostatted 8-Cell Changer for
Ultrospec 7000/8000/9000
8
Code number
80-7100-08
Ultrospec 7000, 8000, and 9000 accessories
Accessories
Peltier Thermocirculator
•Range 20°C to 60°C
•Temperature accuracy of ± 0.1°C
•User-friendly interface and digital display
•Display current and set temperatures
•Sealed unit
Ordering information
Product
Peltier Thermocirculator (requires suitable thermostatted
cell holder or cell changer)
Code number
80-7100-50
Automatic Sipper System
•Automatic aspiration
•User defined air gap and sip volume
•Waste or return option
•Local or PC controlled
•Ability to calibrate sipped volume
•Choice of tubing kits
Ordering information
Product
Code number
Sipper for Ultrospec 7000/8000/9000
Requires Tubing kit (80-7100-37 or 80-7100-38) &
suitable flowcell
80-7100-36
Standard tubing kit for Ultrospec 7000/8000/9000 sipper
Includes 6 Marprene pump head tubes, PTFE tubing
and silicon connection tubing
80-7100-37
Acid Resistant Tubing kit for Ultrospec 7000/8000/9000 sipper
Includes 6 Viton pump head tubes, PTFE tubing and silicon
connection tubing
80-7100-38
80 µl Quartz Flowcell for sipper 10 mm pathlength
with screw connectors and tubing
80-7100-40
Please refer to the “High performance spectrophotometer
accessories” brochure (29‑0043-70) for full details of
these and other products.
9
Ultrospec 2100 pro
Options for enzyme kinetics
•Preset routines for DNA, RNA, oligonucleotide
quantitation, and purity check
•Combines basic measurement modes with graphical
modes: wavelength scan, absorbance changes with
time, reaction rate determinations, and standard
curves can be displayed and printed
•Store up to 18 user-defined methods
•Includes serial and parallel outputs as standard
(outputs to a range of printers)
Ultrospec 2100 pro.
Ultrospec 2100 pro is a simple-to-use instrument for
nucleic acid measurements and enzyme kinetics.
It provides stored routines for nucleic acid quantitation
and a standard curve routine for protein determination,
in addition to measuring absorbance and concentration.
An 8-position sample changer is supplied as standard.
Accessories such as a temperature control unit option
are also available. The stand alone instrument can
be upgraded for more sophisticated applications,
as well as data storage with Datrys Life Science or
CFR software and a PC.
•GLP self-test diagnostics
Spreadsheet interface software
Scans of absorbance/wavelength may be converted to
a more conventional graph by using the spreadsheet
interface software to export results directly to
Microsoft® Excel®. The results can then be formatted or
manipulated as appropriate.
Ordering information
Product
Code number
Ultrospec 2100 pro
Classic
80-2112-21
Yellow
80-2112-22
Plum
80-2112-27
Accessories
10
Spreadsheet interface software
(Requires serial interface cable - sold separately).
80-2110-73
RS232C Serial Interface Cable
80-2105-97
NanoVue Plus spectrophotomer
Low volumes made easy
Nanovue Plus is an easy-to-use instrument for
reliable, low volume, measurement in a wide range of
life science applications
•Eliminate the need for cuvettes, capillaries or other
sample devices—just drop and read
•Conserve precious samples by assaying low volumes
of 0.5 to 5 µl. Samples can also be simply recovered
with a pipette if required
•New PC control option using Datrys
software upgrade
•Predefined methods for nucleic acid and protein
quantitation (including Bradford and Lowry)
•Intuitive software includes automatic self-calibration
on startup
•Choice of data output options including USB cable,
Bluetooth connectivity, integrated printer option and
SD card option
•Built in methods for Calibration curves, Kinetics,
CyDye and fluor incorporation, and Wavelength
scanning across 200 to 900 nm
NanoVue Plus spectrophotometer showing drop, measure, and read
capability. Pipette samples directly onto the novel sample plate for
measurement. The sample plate can then be easily and quickly wiped
clean for the next sample measurement.
Ordering information
Product
Code number
NanoVue Plus
Product
Code number
Accessories
NanoVue Plus with integrated printer
(US & CA only)
28-9569-63
NanoVue Plus with integrated printer
(Worldwide except US & CA)
28-9569-66
NanoVue Plus, print via computer
(US & CA only)
28-9569-62
NanoVue Plus, print via computer
(Worldwide except US & CA)
28-9569-65
NanoVue Plus with Bluetooth connectivity
(US & CA only)
28-9569-64
NanoVue Plus with Bluetooth connectivity
(Worldwide except US & CA)
28-9569-67
NanoVue Plus with SD card option
(US & CA only)
28-9569-61
NanoVue Plus with SD card option
(Worldwide except US & CA)
28-9569-60
Pathlength calibration kit
28-9244-05
Replacement sample plate kit
28-9569-58
Spare printer paper 20 rolls
28-9182-26
Printer accessory
28-9182-27
Lower sample plate kit
28-9569-59
SD card accessory
28-9432-14
Bluetooth accessory
28-9182-25
11
SimpliNano
Accurate low volume measurements
SimpliNano is a fixed patlength microvolume
spectrophotometer for straightforward concentration
and purity measurements of nucleic acids and proteins
(A280). There are no moving parts in SimpliNano, making
it reliable and easy to maintain, with excellent
instrument accuracy.
•Superb data reproducibility: fixed 0.5 mm pathlength
means consistent results time after time
•Increased efficiency in the lab: no pathlength
calibration required due to robust design with no
moving parts
•Easy to use; samples can be added and removed with
no pathlength selection required (one pathlength)
•Inbuilt sample port–just drop in sample and measure
•Conserve precious samples by measuring volumes
as low as 1 to 5 µl
•Preprogrammed methods for nucleic acids and
A280 protein
•Delivers purity data and a spectral scan of DNA
or protein, for added confidence in results.
•Choice of data output - including USB cable to
PC and USB stick as standard, or integrated
printer option
SimpliNano with optional integrated printer.
•Compatible with Datrys software for optional
PC control
Ordering information
Product
Code number
SimpliNano
12
SimpliNano (Worldwide except US and Canada)
29-0617-11
SimpliNano with printer (Worldwide except US and Canada)
29-0617-12
SimpliNano (North America only)
29-0617-13
SimpliNano with printer (North America only)
29-0617-14
SimpliNano spectrophotometer showing sample measurement
port. Samples are pipetted directly into the port for measurement
and then easily removed, leaving the port clean for the next sample.
GeneQuant 1300
Compact, convenient and flexible
GeneQuant 1300 spectrophotometer is a compact
instrument preprogrammed with a range of methods
for the quantitation of proteins, nucleic acids, and
bacterial cell cultures. Absorbance and concentration
measurements can be made at any wavelength,
providing flexibility for future applications.
•Built-in applications for cell density, nucleic acids,
proteins, and enzyme kinetics
•New PC control option using Datrys software upgrade
•cDNA application software for measuring
incorporation of Cy™3 and Cy5, or other dyes
into purified microarray hybridization probes
and PCR products
•Wavelength range of 190 to 1100 nm, with
wavelength scan from 200 to 900 nm performed in
less than 5 s with zoom facility, peak identification,
and on-peak confirmation
•Visual inspection of a nucleic acid scan can identify
the presence of impurities especially useful with
RNA samples
•Optional integrated printer
•USB cable and Print Via Computer (PVC) software
supplied as standard. Bluetooth accessory (optional)
•Three-year lamp warranty
The GeneQuant 100 is also available, with built in applications for nucleic
acids, proteins and cell density measurements.
GeneQuant 1300 can be used with a wide variety of cuvettes or
capillaries for sample volumes from 3 μl to 2 ml.
Ordering information
Product
Code number
GeneQuant 1300
GeneQuant 1300 Classic
28-9182-13
GeneQuant 1300 with printer
28-9182-14
GeneQuant 1300 with Bluetooth
28-9182-15
Bluetooth accessory
28-9182-25
Spare printer paper 20 rolls
28-9182-26
Printer accessory
28-9182-27
GeneQuant 100
GeneQuant 100 Classic
28-9182-04
GeneQuant 100 Classic with Printer
28-9182-05
Spare printer paper 20 rolls
28-9182-26
Printer accessory
28-9182-27
PVC Software for GeneQuant 100
28-9231-88
Capillary cell holder
80-2120-19
Spare capillaries for cell holder
80-2104-67
13
Novaspec Plus and Novaspec III
Basic enzyme kinetics
Novaspec Plus (left) and III (right) include removable cell/test holders for
easy cleaning. The Grafico software supplied allows downloads to PC.
Novaspec Plus
Novaspec III
A good value instrument for general laboratory use, it
includes stored methods for protein quantitation and
enzyme kinetics, plus the basic modes of absorbance,
transmittance, OD600, and concentration.
A light-weight instrument used for measuring
absorbance, % transmission, concentration, and rate.
It is easy to use—just select wavelength, set reference
and measure sample—making it the instrument of
choice for teaching laboratories.
•Kinetics for enzyme studies
•Stored protein methods for Bradford, BCA, Biuret, and Lowry
•Bacterial cell culture measurement at OD600
•“Flash Scan” diode array, for rapid wavelength scans
•Graphical display of wavelength scans, kinetic assays
(including slope calculation for rate/activity studies)
and standard curves
•Option for temperature control (factory fitted)
•Up to 99 stored methods
•Large display with easy-to-read characters
•Absorbance, % transmission, concentration,
factor, and rate
•Ability to measure two wavelengths simultaneously
for kinetics assays
•Self test on startup
•Analog output for connection to chart recorder
•Three student-friendly experiments plus a tutorial on UV-Visible spectrophotometry are included
Ordering information
Product
Code number
Novaspec Plus
80-2117-50
Novaspec Plus with heated cell holder: includes Grafico PC
utility software, serial cable, dust cover.
80-2117-51
Novaspec III
14
Code number
Accessories and other items
Novaspec Plus
Novaspec III
Product
80-2118-00
Test tube adapters (10, 12, 16 mm)
80-2117-47
Chart recorder interface cable
80-3003-55
Lamp Assembly. Tungsten
80-2115-33
Ultrospec 10 Cell Density Meter
Hand-held cell density meter
A small, portable, and dedicated cell density OD600
measurement instrument, the Ultrospec 10 measures
the density of cells (E. coli, other bacteria, and yeast*) in
suspension at 600 nm. This battery-powered, handheld device can be easily used in incubation cabinets,
under anaerobic conditions and in other areas of the
laboratory where cells are cultured. Spillages are easily
wiped from the smooth surface, and the cell
compartment area can be sterilized.
Rechargeable batteries provide nearly one month of
cord-free use.
•Battery operated for use in cell culture facilities
•Easy to use, easy to clean, easy to sterilize
•Download results to a PC or printer for convenient
data storage and retrieval
*(not recommended for use with mammalian cell lines)
Ordering information
Product
Code number
Ultrospec 10 Cell Density Meter
Classic
80-2116-30
Accessories
S2000P Serial Printer (includes serial cable)
80-3000-94
Spare paper for S2000P printer
28-9582-73
15
Datrys System control software
PC control software
For many users, having an external PC to control their
spectrophotometer and manipulate data, gives them
the ultimate in flexibility and control. Whether looking
for small differences in multiple spectral overlays,
carrying out post-run manipulations on large numbers
of samples, GE Healthcare’s Datrys software has the
flexibility to work in the way you want.
Datrys software is supplied in different modules
to meet the application requirements of different user
groups and offers a simple upgrade path should your
requirements change.
Choose:
•Datrys Lite for Quick Read and fast scanning only
•Datrys for all routine measurements
•Datrys Life Science for nucleic acids, proteins and cell density measurements
•Datrys CFR supports 21 CFR part 11 compatibility
All modules are designed with a modern interface that
is familiar from Microsoft Office™ products to reduce
learning curves, and are compatible with Windows® XP,
Windows Vista®, Windows 7 and Windows 8. Data
export options include Microsoft Word and Excel plus
Adobe™ PDF formats.
Datrys software wavelength scan.
Modules
Quick Read and Quick Scan
Datrys Lite
Datrys
•
•
•
•
•
Wavelength scanning, kinetics, quantitative calibration curves
•
Validation
Method developer
Datrys
Life Science
•
•
•
•
•
•
Life science methods
•
Accessory control
21 CFR Part 11 Compatibility
Datrys CFR
•
•
•
•
•
•
•
Ordering information
Product
Code number
Datrys
‡
16
Code number
29-0036-21
Datrys CFR
Datrys Lite
29-0036-22
Datrys Life Science Upgrade
29-0036-25
Datrys Life Science
29-0036-23
Datrys CFR Upgrade
29-0036-26
†
†
Product
29-0036-24
‡
‡
GeneQuant 100/1300, NanoVue, Ultrospec 2100 pro, Ultrospec 7000 upgrade for instruments without Datrys standard installed.
For users who own Datrys standard software
Datrys System control software
Software
modules
Single
wavelength
measurement
Wavelength
scanning
Concentration
standard curve
Kinetics
Stand-alone
instrument software
Custom
method
development
21 CFR Part 11
Compatibilty
Datrys
Datrys
Life Science
Datrys
CFR
Fixed wavelength, Abs/%T,
Concentration (factor)
Single or multiple wavelength measurements in absorbance,
% transmission or concentration including full instrument control,
batch processing of sample data and calculations on collected data
Sample overlays, automatic feature detection
and post run data manipulation
Sample overlays, automatic feature detection and post run data
manipulation, spectral scans and spectral arithmetic manipulations
Quantitation using
calibration curves with
multiple standards and
a choice of curve fits
Generation of calibration curves including full instrument
and accessory control, choice of line fit, QC limit testing
and statistical analysis of sample data
Serial (or parallel
measurements with
the optional cell
changer) including
sample overlays
and post run
data manipulation
Absorbance versus time measurements on single or multiple cells (with
optional cell changer), multiple sample overlays, batch processing of
sample data and post run sample calculations and manipulations
Validation
Life science
methods
Datrys
Lite
Software module that allows users to enter and store full details of their Certified Reference
Materials (CRMs) and schedule tests to be carried out at defined intervals to meet their Standard
Operating Procedures. Users are prompted to insert the specified CRM at the scheduled time
and the system automatically checks the performance against the instrument specification.
DNA, RNA & Oligo
concentration and
purity measurement
with optional
wavelength scanning.
CyDye DNA
quantitation.
Tm calculation.
Protein concentration
by direct
UV measurement
or Bradford, Biuret,
Lowry or BCA
colorimetric methods
Equation Editor
allows the set up
of stored methods
that can include
calculations based on
measured sample data
e.g., Absorbance ratio
and difference.
DNA, RNA and Oligo concentration
and purity measurement
Protein quantitation via direct UV
methods or BCA, Buiret, Bradford,
Lowry and Colloidal Gold methods
Cell Density measurement
Fluorescent dye methods
The Method Developer module allows custom methods to be set
up in all applications. These can include sample prompts
containing information such as method SOP timings and the
ability to lock individual method parameters
Ability to set up
different users as
part of defined
groups.
Audit trail.
Electronic signature
17
Software and qualification packages
Print Via Computer (PVC) software
Print Via Computer software runs under Windows
2000, Windows XP, Window Vista, Windows 7, and
Windows 8, and enables transfer of data into a PC
environment using a USB cable, Bluetooth, or SD card.
PVC is capable of supporting several instruments
simultaneously, and is compatible with
GeneQuant 100 and 1300, NanoVue Plus, SimpliNano,
Ultrospec 7000, 8000 and 9000 stand alone units.
Data can be printed, saved or exported in a variety
of formats (including Microsoft Excel) either manually
or automatically.
Summary of software by instrument
18
Instrument
Standard Software Optional Packages
Instrument
Standard Software Optional Packages
Ultrospec 7000PC
Datrys Standard
Datrys Life Science or
CFR Upgrade version
Ultrospec 2100 pro
None
Datrys Lite, Standard,
Life Science, CFR Full versions
Ultrospec 8000PC
Datrys Standard
Datrys Life Science or
CFR Upgrade version
GeneQuant 100
None
PVC, Datrys Lite, Standard,
Life Science, CFR Full versions
Ultrospec 9000PC
Datrys Standard
Datrys Life Science or
CFR Upgrade version
GeneQuant 1300
PVC
Datrys Lite, Standard,
Life Science, CFR Full versions
Ultrospec 7000
(Stand alone)
PVC only
Datrys Lite, Standard,
Life Science, CFR Full versions
NanoVue Plus
PVC
Datrys Lite, Standard,
Life Science, CFR Full versions
Ultrospec 8000
(Stand alone)
PVC &
Datrys Standard
Datrys Life Science or
CFR Upgrade version
SimpliNano
PVC
Datrys Lite, Standard, Life
Science, CFR Full versions
Ultrospec 9000
(Stand alone)
PVC &
Datrys Standard
Datrys Life Science or
CFR Upgrade version
Novaspec III/Plus
Grafico
None
Ultrospec 10
None
None
Related products
Accessories for Ultrospec 2100 pro
Pack size
Code number
4-position cell changer (10 – 50 mm pathlength)
1
80-2106-01
6-position Peltier heated cell changer, 10 mm
pathlength (requires Temperature Control
Unit, see 80-2105-49)
1
80-2106-04
8-position water heated cell changer, 10 mm
pathlength (requires circulation bath)
1
80-2109-70
6-position Peltier heated cell changer.
Sipper, complete with UV quartz cell and all
tubing (requires single cell holder 80-2106-05
or 80-2106-13)
1
80-2112-15
Temperature Control Unit
1
80-2105-49
Lens cleaning tissue
25 × 25 sheets
2105-841
For a complete list of accessories, including cells and cell holders, please go to
www.gelifesciences.com/spectros
Nucleic acid preparation products
Product
Pack size Code number
illustra RNAspin Mini Kit
20
25-0500-70
illustra RNAspin Mini Kit
50
25-0500-71
illustra RNAspin Midi Kit
20
25-0500-73
illustra tissue and cells genomicPrep Mini Spin Kit
50
28-9042-75
illustra tissue and cells genomicPrep Mini Spin Kit
250
28-9042-76
illustra tissue and cells genomicPrep Midi Flow Kit
25
28-9042-73
illustra blood genomicPrep Mini Spin Kit
50
28-9042-64
illustra blood genomicPrep Mini Spin Kit
250
28-9042-65
illustra blood genomicPrep Midi Flow Kit
25
28-9042-61
illustra blood genomicPrep Midi Flow Kit
100
28-9042-62
illustra bacteria genomicPrep Mini Spin Kit
50
28-9042-58
illustra bacteria genomicPrep Mini Spin Kit
250
28-9042-59
illustra plasmidPrep Mini Spin Kit
50
28-9042-69
illustra plasmidPrep Mini Spin Kit
250
28-9042-70
illustra plasmidPrep Midi Flow Kit
25
28-9042-67
illustra plasmidPrep Midi Flow Kit
100
28-9042-68
illustra triplePrep Kit (for DNA, RNA & protein)
50
28-9425-44
™
illustra blood genomicPrep Mini Spin Kit.
illustra plasmidPrep Mini Spin Kit.
Ab SpinTrap.
Protein sample preparation products
Product
Preparation
Pack size
Code number
Disposable PD-10 Desalting columns
Protein desalting and buffer exchange
30
17-0851-01
Ab SpinTrap
Antibody purification and protein enrichment using Protein G as ligand
50
28-4083-47
4 × 500 µl
28-9513-78
Separation tool for handling magnetic beads in microcentrifuge tubes,
up to six samples processed in parallel
1
28-9489-64
2-D Clean-Up Kit
Removal of interfering contaminants and concentration of total protein
50
80-6484-51
SDS-PAGE Clean-Up Kit
Removal of interfering contaminants
50
80-6484-70
His SpinTrap
Purification of histidine-tagged proteins
50
28-4013-53
™
Protein A Mag Sepharose
™
MagRack 6
Magnetic beads for protein enrichment by immunoprecipitation
applications using Protein A as ligand
www.gelifesciences.com/spectros
GE Healthcare UK Limited, Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK
GE and GE monogram are trademarks of General Electric Company.
Cy, CyDye, illustra, NanoVue, SimpliNano, Sepharose, and SpinTrap are trademarks of General Electric Company or one of its subsidiaries.
Adobe is a trademark of Adobe Systems Incorporated. BCA is a trademark of Thermo Fisher Scientific LLC. Bluetooth is a trademark of Bluetooth SIG. Excel, Microsoft,
Windows, and Windows Vista are registered trademarks of Microsoft Corporation. GeneQuant, Novaspec, and Ultrospec are trademarks of Biochrom Ltd.
All other third party trademarks are the property of their respective owners.
© 2009 -2014 General Electric Company—All right reserved. First published Dec. 2009.
GE Healthcare Bio-Sciences AB, Björkgatan 30, 751 84 Uppsala, Sweden
GE Healthcare Europe, GmbH, Munzinger Strasse 5, D-79111 Freiburg, Germany
GE Healthcare Bio-Sciences Corp., 800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327 USA
GE Healthcare Japan Corporation, Sanken Bldg., 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073 Japan
28-9271-31 AD 08/2014