Now the only difficult thing about spectrophotometry is how to say it www.gelifesciences.com 1 Contents Introduction3 Spectrophotometer selection guide 4 High performance analytics Ultrospec™ 9000 Ultrospec 8000 Ultrospec 7000 Ultrospec 7000, 8000 & 9000 Accessories 2 5 6 7 8 Options for enzyme kinetics Ultrospec 2100 pro 10 Low volumes NanoVue™ Plus SimpliNano™ Fixed pathlength 11 12 Compact, convenient, and flexible GeneQuant™ 1300 13 Basic enzyme kinetics Novaspec™ Plus Novaspec III 14 14 Hand-held cell density meter Ultrospec 10 Cell Density Meter 15 System control software Datrys System Contol Software 16 Software and qualification packages Print Via Computer (PVC) software Summary of software by instrument 18 18 Related products 19 Introduction UV-Visible spectrophotometers are a ubiquitous tool in the life sciences because of the wide range of molecules that can be detected and analyzed using this technique. Like many other everyday items, it’s easy to forget how long you have had your spectrophotometer and you might not think about its performance or capabilities until you need to replace it. Questions to consider include: •What applications are routinely run in your laboratory? Could this change in the near future? •What range of sample volumes do you need to measure? If this varies, look for options for different cuvette sizes and adaptors •Do you want to measure multiple samples at the same time? If so, consider options for multiple cell holders •What level of sensitivity do you need? For low concentration samples, a wider bandwidth (3 to 5 nm) will give more consistent results than a narrow (1 nm) bandwidth instrument •How many users will there be and what level of experience will they have? Preprogrammed methods and password control of your own methods can be useful features •How will data be recorded, output or stored? This guide is an introduction to the range of spectrophotometers available from GE Healthcare Life Sciences. Whether you are performing simple colorimetric assays or conducting the most stringent research, there is an instrument from our range to meet your needs. Low sample volumes NanoVue Plus, with dual pathlengths, offers both convenience and a wide dynamic range for life science applications for volumes 0.5 to 5.0 µl. SimpliNano fixed pathlength spectrophotometer offers superb accuracy for nucleic acid and protein A280 measurements, as low as 1 µl. The ability to pipette samples directly onto the sample plate (NanoVue Plus) or port (SimpliNano) eliminates the need for cuvettes or other sample devices. Routine applications GE Healthcare offers a number of spectrophotometers for widely used applications such as DNA and RNA quantitation, protein concentration determination, and cell density measurements. This includes Ultrospec™ 10, a battery-operated, portable, cell density meter; Novaspec III and Plus for protein determination and/or enzyme kinetics; and GeneQuant 100 and 1300 instruments. Additional features For more advanced features, Ultrospec 2100 offers temperature control and cell changer options, while the Ultrospec 8000 and 9000 are high performance, dual-beam instruments, for precision measurements that are compatible with Pharmacopoeia guidelines. Data output Many GE Healthcare spectrophotometers have a variety of data output capabilities. These include integrated printer, USB cable to PC, USB stick or Bluetooth™ wireless options. 3 High-performance dual-beam, 2 nm bandwidth UV-Visible spectrophotometer. Wavelength: 190-1100 nm. Recommended for Life Science applications, with optional Life Science or CFR Datrys software upgrades. Ultrospec 2100 3 nm bandwidth instrument. Wavelength: 190-900nm, with 8-position cell changer and options for temperature control NanoVue Plus 2 pathlength sample mechanism eliminates need for cuvettes, facilitates low-volume measurements (0.5 to 5 µl) and gives wide dynamic range across life science applications SimpliNano Fixed pathlength low volume (1 to 5 µl) measurements for nucleic acids and Protein A280 GeneQuant 1300 As GeneQuant 100 and with built-in enzyme kinetics, and CyDye™ applications plus Bluetooth data output options GeneQuant 100 5 nm bandwidth instrument with built in applications for nucleic acids, proteins and cell density Novaspec Plus As Novaspec III and with stored protein methods, easy to read graphical display, and temperature control option Novaspec III 7 nm bandwidth visible spectrophotometer, 330 to 800 nm Ultrospec 10 Battery-powered, portable dedicated cell density meter = recommended; 4 = suitable; = not suitable Pharma method development Ultrospec 7000 European Pharmacopeia compatibility High-performance dual-beam, 1 nm bandwidth UV-Visible spectrophotometer. Wavelength: 190-1100 nm. Recommended for Pharma, QC and analytical labs. CFR Datrys software upgrades available. Life Science applications, with optional Life Science or CFR Datrys software upgrades. Fluorescent probe quantitation, cDNA probes for microarrays, PCR probes Ultrospec 8000 Enzyme activity kinetics at 340 nm High-performance dual-beam, variable bandwidth UV-Visible spectrophotometer. Wavelength: 190-1100nm. Recommended for Pharma, QC and analytical labs. CFR Datrys software upgrades available. Cell culture OD600 Ultrospec 9000 PCR/sequencing/ hybridization primer quantitation and design Main features Color protein quantitation (Bradford, Biuret, BCA™ & Lowry) Model DNA/RNA & direct (A280) protein quantitation Spectrophotometer selection guide Ultrospec 9000/9000PC UV-Visible spectrophotometer Dual beam High performance method development Ultrospec 9000/9000PC is a dual-beam UV‑Visible spectrophotometer for operation in high‑specification laboratories. The instrument is European Pharmacopeia compatible and is also compatible with CFR part 11 requirements via the optional Datrys software. Method development is supported through variable bandwidth capability and custom calculation facilities. Key features •Dual-beam, variable bandwidth optics—high performance for difficult samples •Full European Pharmacopeia compatibility Ultrospec 9000 with PC-control option. •21 CFR part 11 support through Datrys CFR (optional) •Custom calculation facility for method development using Datrys software •Equation Editor software allowing custom calculations to be applied to measured data •Color touchscreen (stand-alone instrument)— simpler operation •Software for wavelength scanning, fixed wavelength, standard curves, kinetics and prestored life science methods as standard •Stand-alone version also includes Print Via Computer (PVC) •Wide range of accessories and options available including cell changers, thermostatting, sipper, built-in printer, Bluetooth and Datrys software options. Please refer to the “High performance spectrophotometer accessories” brochure (29‑0043-70) for additional information •Multiuser analytical instrument with secure login to protect methods •USB memory stick data/method storage and USB PC connection—export data the way you want Ordering information •Stand-alone or PC-controlled versions available Product •Both instrument versions are supplied with Datrys Standard, and can be upgraded to Datrys Life Science or Datrys CFR Ultrospec 9000 UV-Visible Spectrophotometer 29-0036-16 Ultrospec 9000PC UV-Visible Spectrophotometer 29-0036-17 Ultrospec 9000 UV-Visible Spectrophotometer with printer accessory 29-0036-18 Ultrospec 9000 UV-Visible Spectrophotometer with Bluetooth accessory 29-0036-19 Ultrospec 9000 UV-Visible Spectrophotometer with printer and Bluetooth accessory 29-0036-20 Code number 5 Ultrospec 8000/8000PC UV-Visible spectrophotometer Dual beam High performance analytics Ultrospec 8000/8000PC is a dual-beam UV‑Visible spectrophotometer designed to meet the requirements of critical analysis and users in Pharmacopeia research environments. Secure user log in and method control, plus a 1 nm bandwidth enables European Pharmacopeia compatability. 21 CFR part 11 support can be achieved with the optional Datrys CFR software. Key features •Dual-beam instrument, with a 1 nm bandwidth •Full European Pharmacopeia compatibility •21 CFR part 11 support through Datrys CFR (optional) •Color touchscreen (stand-alone instrument)— simpler operation •Software for wavelength scanning, fixed wavelength, standard curves, kinetics and prestored life science methods as standard •Equation Editor software allowing custom calculations to be applied to measured data •Multiuser analytical instrument with secure login to protect methods •USB memory stick data/method storage and USB PC connection—export data the way you want •Stand-alone or PC-controlled versions available •Both instrument versions are supplied with Datrys Standard. Can be upgraded to Datrys Life Science or Datrys CFR •Stand-alone version also includes Print Via Computer (PVC) Ultrospec 8000 stand-alone instrument. Ordering information Product 6 Code number Ultrospec 8000 UV-Visible Spectrophotometer 29-0036-10 Ultrospec 8000PC UV-Visible Spectrophotometer 29-0036-11 Ultrospec 8000 UV-Visible Spectrophotometer with printer accessory 29-0036-12 Ultrospec 8000 UV-Visible Spectrophotometer with Bluetooth accessory 29-0036-13 Ultrospec 8000 UV-Visible Spectrophotometer with printer and Bluetooth accessory 29-0036-14 •Wide range of accessories and options available including cell changers, thermostatting, sipper, built-in printer, Bluetooth and Datrys software options. Please refer to the “High performance spectrophotometer accessories” brochure (29‑0043-70) for additional information Ultrospec 7000/7000PC UV-Visible spectrophotometer Dual beam High performance multiple applications Ultrospec 7000/7000PC is a multiple application, dual-beam, UV‑Visible spectrophotometer developed to meet the day-to-day needs of the busy laboratory. Engineered for robust, lower cost operation with minimal maintenance requirements, yet providing simple, touchscreen operation, multiple user profiles and a choice of data export methods. A range of software upgrade and accessory options are available to meet your needs in the future. Ultrospec 7000 with PC-control option. Key features •Dual-beam instrument with a 2 nm bandwidth •High-energy Xenon lamp minimizes annual running costs •Color touchscreen (stand-alone instrument)— simpler operation •Software for wavelength scanning, fixed wavelength, standard curves, kinetics and prestored life science methods as standard •Equation Editor software allowing custom calculations to be applied to measured data Ultrospec 7000 stand-alone instrument. •Multiuser analytical instrument with secure login to protect methods •USB memory stick data/method storage and USB PC connection—export data the way you want Ordering information Product Code number •Stand-alone or PC-controlled versions available Ultrospec 7000 UV-Visible Spectrophotometer 29-0036-05 •PC version is supplied with Datrys Standard Ultrospec 7000PC UV-Visible Spectrophotometer 29-0036-06 Ultrospec 7000 UV-Visible Spectrophotometer with printer accessory 29-0036-07 Ultrospec 7000 UV-Visible Spectrophotometer with Bluetooth accessory •Stand-alone version includes Print Via Computer (PVC) only—can be upgraded with Datrys software options including Life Science and CFR 29-0036-08 Ultrospec 7000 UV-Visible Spectrophotometer with printer and Bluetooth accessory 29-0036-09 •Wide range of accessories and options available including cell changers, thermostatting, sipper, built-in printer, Bluetooth and Datrys software options. Please refer to the “High performance spectrophotometer accessories” brochure (29‑0043-70) for additional information 7 Ultrospec 7000, 8000, and 9000 accessories Accessories With a large sample compartment, the Ultrospec instruments can be configured with quick change accessories including sipper, temperature control accessories, long pathlength, test tube and film holders. Film Holder •Holds films from 1 mm to 10 mm thickness •Holds films up to 160 mm wide and 70 mm high •Fits to standard sample compartment baseplate, can be changed in seconds •Holder installed in sample position only Ordering information Product Film holder for Ultrospec 7000/8000/9000 Code number 80-7100-13 Automatic 8-Cell Changer •Fully automatic operation •Fully controlled by software, parameters stored within application methods •Fits to standard sample compartment base plate, can be changed in seconds Ordering information Product Non Thermostatted 8-Cell Changer for Ultrospec 7000/8000/9000 8 Code number 80-7100-08 Ultrospec 7000, 8000, and 9000 accessories Accessories Peltier Thermocirculator •Range 20°C to 60°C •Temperature accuracy of ± 0.1°C •User-friendly interface and digital display •Display current and set temperatures •Sealed unit Ordering information Product Peltier Thermocirculator (requires suitable thermostatted cell holder or cell changer) Code number 80-7100-50 Automatic Sipper System •Automatic aspiration •User defined air gap and sip volume •Waste or return option •Local or PC controlled •Ability to calibrate sipped volume •Choice of tubing kits Ordering information Product Code number Sipper for Ultrospec 7000/8000/9000 Requires Tubing kit (80-7100-37 or 80-7100-38) & suitable flowcell 80-7100-36 Standard tubing kit for Ultrospec 7000/8000/9000 sipper Includes 6 Marprene pump head tubes, PTFE tubing and silicon connection tubing 80-7100-37 Acid Resistant Tubing kit for Ultrospec 7000/8000/9000 sipper Includes 6 Viton pump head tubes, PTFE tubing and silicon connection tubing 80-7100-38 80 µl Quartz Flowcell for sipper 10 mm pathlength with screw connectors and tubing 80-7100-40 Please refer to the “High performance spectrophotometer accessories” brochure (29‑0043-70) for full details of these and other products. 9 Ultrospec 2100 pro Options for enzyme kinetics •Preset routines for DNA, RNA, oligonucleotide quantitation, and purity check •Combines basic measurement modes with graphical modes: wavelength scan, absorbance changes with time, reaction rate determinations, and standard curves can be displayed and printed •Store up to 18 user-defined methods •Includes serial and parallel outputs as standard (outputs to a range of printers) Ultrospec 2100 pro. Ultrospec 2100 pro is a simple-to-use instrument for nucleic acid measurements and enzyme kinetics. It provides stored routines for nucleic acid quantitation and a standard curve routine for protein determination, in addition to measuring absorbance and concentration. An 8-position sample changer is supplied as standard. Accessories such as a temperature control unit option are also available. The stand alone instrument can be upgraded for more sophisticated applications, as well as data storage with Datrys Life Science or CFR software and a PC. •GLP self-test diagnostics Spreadsheet interface software Scans of absorbance/wavelength may be converted to a more conventional graph by using the spreadsheet interface software to export results directly to Microsoft® Excel®. The results can then be formatted or manipulated as appropriate. Ordering information Product Code number Ultrospec 2100 pro Classic 80-2112-21 Yellow 80-2112-22 Plum 80-2112-27 Accessories 10 Spreadsheet interface software (Requires serial interface cable - sold separately). 80-2110-73 RS232C Serial Interface Cable 80-2105-97 NanoVue Plus spectrophotomer Low volumes made easy Nanovue Plus is an easy-to-use instrument for reliable, low volume, measurement in a wide range of life science applications •Eliminate the need for cuvettes, capillaries or other sample devices—just drop and read •Conserve precious samples by assaying low volumes of 0.5 to 5 µl. Samples can also be simply recovered with a pipette if required •New PC control option using Datrys software upgrade •Predefined methods for nucleic acid and protein quantitation (including Bradford and Lowry) •Intuitive software includes automatic self-calibration on startup •Choice of data output options including USB cable, Bluetooth connectivity, integrated printer option and SD card option •Built in methods for Calibration curves, Kinetics, CyDye and fluor incorporation, and Wavelength scanning across 200 to 900 nm NanoVue Plus spectrophotometer showing drop, measure, and read capability. Pipette samples directly onto the novel sample plate for measurement. The sample plate can then be easily and quickly wiped clean for the next sample measurement. Ordering information Product Code number NanoVue Plus Product Code number Accessories NanoVue Plus with integrated printer (US & CA only) 28-9569-63 NanoVue Plus with integrated printer (Worldwide except US & CA) 28-9569-66 NanoVue Plus, print via computer (US & CA only) 28-9569-62 NanoVue Plus, print via computer (Worldwide except US & CA) 28-9569-65 NanoVue Plus with Bluetooth connectivity (US & CA only) 28-9569-64 NanoVue Plus with Bluetooth connectivity (Worldwide except US & CA) 28-9569-67 NanoVue Plus with SD card option (US & CA only) 28-9569-61 NanoVue Plus with SD card option (Worldwide except US & CA) 28-9569-60 Pathlength calibration kit 28-9244-05 Replacement sample plate kit 28-9569-58 Spare printer paper 20 rolls 28-9182-26 Printer accessory 28-9182-27 Lower sample plate kit 28-9569-59 SD card accessory 28-9432-14 Bluetooth accessory 28-9182-25 11 SimpliNano Accurate low volume measurements SimpliNano is a fixed patlength microvolume spectrophotometer for straightforward concentration and purity measurements of nucleic acids and proteins (A280). There are no moving parts in SimpliNano, making it reliable and easy to maintain, with excellent instrument accuracy. •Superb data reproducibility: fixed 0.5 mm pathlength means consistent results time after time •Increased efficiency in the lab: no pathlength calibration required due to robust design with no moving parts •Easy to use; samples can be added and removed with no pathlength selection required (one pathlength) •Inbuilt sample port–just drop in sample and measure •Conserve precious samples by measuring volumes as low as 1 to 5 µl •Preprogrammed methods for nucleic acids and A280 protein •Delivers purity data and a spectral scan of DNA or protein, for added confidence in results. •Choice of data output - including USB cable to PC and USB stick as standard, or integrated printer option SimpliNano with optional integrated printer. •Compatible with Datrys software for optional PC control Ordering information Product Code number SimpliNano 12 SimpliNano (Worldwide except US and Canada) 29-0617-11 SimpliNano with printer (Worldwide except US and Canada) 29-0617-12 SimpliNano (North America only) 29-0617-13 SimpliNano with printer (North America only) 29-0617-14 SimpliNano spectrophotometer showing sample measurement port. Samples are pipetted directly into the port for measurement and then easily removed, leaving the port clean for the next sample. GeneQuant 1300 Compact, convenient and flexible GeneQuant 1300 spectrophotometer is a compact instrument preprogrammed with a range of methods for the quantitation of proteins, nucleic acids, and bacterial cell cultures. Absorbance and concentration measurements can be made at any wavelength, providing flexibility for future applications. •Built-in applications for cell density, nucleic acids, proteins, and enzyme kinetics •New PC control option using Datrys software upgrade •cDNA application software for measuring incorporation of Cy™3 and Cy5, or other dyes into purified microarray hybridization probes and PCR products •Wavelength range of 190 to 1100 nm, with wavelength scan from 200 to 900 nm performed in less than 5 s with zoom facility, peak identification, and on-peak confirmation •Visual inspection of a nucleic acid scan can identify the presence of impurities especially useful with RNA samples •Optional integrated printer •USB cable and Print Via Computer (PVC) software supplied as standard. Bluetooth accessory (optional) •Three-year lamp warranty The GeneQuant 100 is also available, with built in applications for nucleic acids, proteins and cell density measurements. GeneQuant 1300 can be used with a wide variety of cuvettes or capillaries for sample volumes from 3 μl to 2 ml. Ordering information Product Code number GeneQuant 1300 GeneQuant 1300 Classic 28-9182-13 GeneQuant 1300 with printer 28-9182-14 GeneQuant 1300 with Bluetooth 28-9182-15 Bluetooth accessory 28-9182-25 Spare printer paper 20 rolls 28-9182-26 Printer accessory 28-9182-27 GeneQuant 100 GeneQuant 100 Classic 28-9182-04 GeneQuant 100 Classic with Printer 28-9182-05 Spare printer paper 20 rolls 28-9182-26 Printer accessory 28-9182-27 PVC Software for GeneQuant 100 28-9231-88 Capillary cell holder 80-2120-19 Spare capillaries for cell holder 80-2104-67 13 Novaspec Plus and Novaspec III Basic enzyme kinetics Novaspec Plus (left) and III (right) include removable cell/test holders for easy cleaning. The Grafico software supplied allows downloads to PC. Novaspec Plus Novaspec III A good value instrument for general laboratory use, it includes stored methods for protein quantitation and enzyme kinetics, plus the basic modes of absorbance, transmittance, OD600, and concentration. A light-weight instrument used for measuring absorbance, % transmission, concentration, and rate. It is easy to use—just select wavelength, set reference and measure sample—making it the instrument of choice for teaching laboratories. •Kinetics for enzyme studies •Stored protein methods for Bradford, BCA, Biuret, and Lowry •Bacterial cell culture measurement at OD600 •“Flash Scan” diode array, for rapid wavelength scans •Graphical display of wavelength scans, kinetic assays (including slope calculation for rate/activity studies) and standard curves •Option for temperature control (factory fitted) •Up to 99 stored methods •Large display with easy-to-read characters •Absorbance, % transmission, concentration, factor, and rate •Ability to measure two wavelengths simultaneously for kinetics assays •Self test on startup •Analog output for connection to chart recorder •Three student-friendly experiments plus a tutorial on UV-Visible spectrophotometry are included Ordering information Product Code number Novaspec Plus 80-2117-50 Novaspec Plus with heated cell holder: includes Grafico PC utility software, serial cable, dust cover. 80-2117-51 Novaspec III 14 Code number Accessories and other items Novaspec Plus Novaspec III Product 80-2118-00 Test tube adapters (10, 12, 16 mm) 80-2117-47 Chart recorder interface cable 80-3003-55 Lamp Assembly. Tungsten 80-2115-33 Ultrospec 10 Cell Density Meter Hand-held cell density meter A small, portable, and dedicated cell density OD600 measurement instrument, the Ultrospec 10 measures the density of cells (E. coli, other bacteria, and yeast*) in suspension at 600 nm. This battery-powered, handheld device can be easily used in incubation cabinets, under anaerobic conditions and in other areas of the laboratory where cells are cultured. Spillages are easily wiped from the smooth surface, and the cell compartment area can be sterilized. Rechargeable batteries provide nearly one month of cord-free use. •Battery operated for use in cell culture facilities •Easy to use, easy to clean, easy to sterilize •Download results to a PC or printer for convenient data storage and retrieval *(not recommended for use with mammalian cell lines) Ordering information Product Code number Ultrospec 10 Cell Density Meter Classic 80-2116-30 Accessories S2000P Serial Printer (includes serial cable) 80-3000-94 Spare paper for S2000P printer 28-9582-73 15 Datrys System control software PC control software For many users, having an external PC to control their spectrophotometer and manipulate data, gives them the ultimate in flexibility and control. Whether looking for small differences in multiple spectral overlays, carrying out post-run manipulations on large numbers of samples, GE Healthcare’s Datrys software has the flexibility to work in the way you want. Datrys software is supplied in different modules to meet the application requirements of different user groups and offers a simple upgrade path should your requirements change. Choose: •Datrys Lite for Quick Read and fast scanning only •Datrys for all routine measurements •Datrys Life Science for nucleic acids, proteins and cell density measurements •Datrys CFR supports 21 CFR part 11 compatibility All modules are designed with a modern interface that is familiar from Microsoft Office™ products to reduce learning curves, and are compatible with Windows® XP, Windows Vista®, Windows 7 and Windows 8. Data export options include Microsoft Word and Excel plus Adobe™ PDF formats. Datrys software wavelength scan. Modules Quick Read and Quick Scan Datrys Lite Datrys • • • • • Wavelength scanning, kinetics, quantitative calibration curves • Validation Method developer Datrys Life Science • • • • • • Life science methods • Accessory control 21 CFR Part 11 Compatibility Datrys CFR • • • • • • • Ordering information Product Code number Datrys ‡ 16 Code number 29-0036-21 Datrys CFR Datrys Lite 29-0036-22 Datrys Life Science Upgrade 29-0036-25 Datrys Life Science 29-0036-23 Datrys CFR Upgrade 29-0036-26 † † Product 29-0036-24 ‡ ‡ GeneQuant 100/1300, NanoVue, Ultrospec 2100 pro, Ultrospec 7000 upgrade for instruments without Datrys standard installed. For users who own Datrys standard software Datrys System control software Software modules Single wavelength measurement Wavelength scanning Concentration standard curve Kinetics Stand-alone instrument software Custom method development 21 CFR Part 11 Compatibilty Datrys Datrys Life Science Datrys CFR Fixed wavelength, Abs/%T, Concentration (factor) Single or multiple wavelength measurements in absorbance, % transmission or concentration including full instrument control, batch processing of sample data and calculations on collected data Sample overlays, automatic feature detection and post run data manipulation Sample overlays, automatic feature detection and post run data manipulation, spectral scans and spectral arithmetic manipulations Quantitation using calibration curves with multiple standards and a choice of curve fits Generation of calibration curves including full instrument and accessory control, choice of line fit, QC limit testing and statistical analysis of sample data Serial (or parallel measurements with the optional cell changer) including sample overlays and post run data manipulation Absorbance versus time measurements on single or multiple cells (with optional cell changer), multiple sample overlays, batch processing of sample data and post run sample calculations and manipulations Validation Life science methods Datrys Lite Software module that allows users to enter and store full details of their Certified Reference Materials (CRMs) and schedule tests to be carried out at defined intervals to meet their Standard Operating Procedures. Users are prompted to insert the specified CRM at the scheduled time and the system automatically checks the performance against the instrument specification. DNA, RNA & Oligo concentration and purity measurement with optional wavelength scanning. CyDye DNA quantitation. Tm calculation. Protein concentration by direct UV measurement or Bradford, Biuret, Lowry or BCA colorimetric methods Equation Editor allows the set up of stored methods that can include calculations based on measured sample data e.g., Absorbance ratio and difference. DNA, RNA and Oligo concentration and purity measurement Protein quantitation via direct UV methods or BCA, Buiret, Bradford, Lowry and Colloidal Gold methods Cell Density measurement Fluorescent dye methods The Method Developer module allows custom methods to be set up in all applications. These can include sample prompts containing information such as method SOP timings and the ability to lock individual method parameters Ability to set up different users as part of defined groups. Audit trail. Electronic signature 17 Software and qualification packages Print Via Computer (PVC) software Print Via Computer software runs under Windows 2000, Windows XP, Window Vista, Windows 7, and Windows 8, and enables transfer of data into a PC environment using a USB cable, Bluetooth, or SD card. PVC is capable of supporting several instruments simultaneously, and is compatible with GeneQuant 100 and 1300, NanoVue Plus, SimpliNano, Ultrospec 7000, 8000 and 9000 stand alone units. Data can be printed, saved or exported in a variety of formats (including Microsoft Excel) either manually or automatically. Summary of software by instrument 18 Instrument Standard Software Optional Packages Instrument Standard Software Optional Packages Ultrospec 7000PC Datrys Standard Datrys Life Science or CFR Upgrade version Ultrospec 2100 pro None Datrys Lite, Standard, Life Science, CFR Full versions Ultrospec 8000PC Datrys Standard Datrys Life Science or CFR Upgrade version GeneQuant 100 None PVC, Datrys Lite, Standard, Life Science, CFR Full versions Ultrospec 9000PC Datrys Standard Datrys Life Science or CFR Upgrade version GeneQuant 1300 PVC Datrys Lite, Standard, Life Science, CFR Full versions Ultrospec 7000 (Stand alone) PVC only Datrys Lite, Standard, Life Science, CFR Full versions NanoVue Plus PVC Datrys Lite, Standard, Life Science, CFR Full versions Ultrospec 8000 (Stand alone) PVC & Datrys Standard Datrys Life Science or CFR Upgrade version SimpliNano PVC Datrys Lite, Standard, Life Science, CFR Full versions Ultrospec 9000 (Stand alone) PVC & Datrys Standard Datrys Life Science or CFR Upgrade version Novaspec III/Plus Grafico None Ultrospec 10 None None Related products Accessories for Ultrospec 2100 pro Pack size Code number 4-position cell changer (10 – 50 mm pathlength) 1 80-2106-01 6-position Peltier heated cell changer, 10 mm pathlength (requires Temperature Control Unit, see 80-2105-49) 1 80-2106-04 8-position water heated cell changer, 10 mm pathlength (requires circulation bath) 1 80-2109-70 6-position Peltier heated cell changer. Sipper, complete with UV quartz cell and all tubing (requires single cell holder 80-2106-05 or 80-2106-13) 1 80-2112-15 Temperature Control Unit 1 80-2105-49 Lens cleaning tissue 25 × 25 sheets 2105-841 For a complete list of accessories, including cells and cell holders, please go to www.gelifesciences.com/spectros Nucleic acid preparation products Product Pack size Code number illustra RNAspin Mini Kit 20 25-0500-70 illustra RNAspin Mini Kit 50 25-0500-71 illustra RNAspin Midi Kit 20 25-0500-73 illustra tissue and cells genomicPrep Mini Spin Kit 50 28-9042-75 illustra tissue and cells genomicPrep Mini Spin Kit 250 28-9042-76 illustra tissue and cells genomicPrep Midi Flow Kit 25 28-9042-73 illustra blood genomicPrep Mini Spin Kit 50 28-9042-64 illustra blood genomicPrep Mini Spin Kit 250 28-9042-65 illustra blood genomicPrep Midi Flow Kit 25 28-9042-61 illustra blood genomicPrep Midi Flow Kit 100 28-9042-62 illustra bacteria genomicPrep Mini Spin Kit 50 28-9042-58 illustra bacteria genomicPrep Mini Spin Kit 250 28-9042-59 illustra plasmidPrep Mini Spin Kit 50 28-9042-69 illustra plasmidPrep Mini Spin Kit 250 28-9042-70 illustra plasmidPrep Midi Flow Kit 25 28-9042-67 illustra plasmidPrep Midi Flow Kit 100 28-9042-68 illustra triplePrep Kit (for DNA, RNA & protein) 50 28-9425-44 ™ illustra blood genomicPrep Mini Spin Kit. illustra plasmidPrep Mini Spin Kit. Ab SpinTrap. Protein sample preparation products Product Preparation Pack size Code number Disposable PD-10 Desalting columns Protein desalting and buffer exchange 30 17-0851-01 Ab SpinTrap Antibody purification and protein enrichment using Protein G as ligand 50 28-4083-47 4 × 500 µl 28-9513-78 Separation tool for handling magnetic beads in microcentrifuge tubes, up to six samples processed in parallel 1 28-9489-64 2-D Clean-Up Kit Removal of interfering contaminants and concentration of total protein 50 80-6484-51 SDS-PAGE Clean-Up Kit Removal of interfering contaminants 50 80-6484-70 His SpinTrap Purification of histidine-tagged proteins 50 28-4013-53 ™ Protein A Mag Sepharose ™ MagRack 6 Magnetic beads for protein enrichment by immunoprecipitation applications using Protein A as ligand www.gelifesciences.com/spectros GE Healthcare UK Limited, Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK GE and GE monogram are trademarks of General Electric Company. Cy, CyDye, illustra, NanoVue, SimpliNano, Sepharose, and SpinTrap are trademarks of General Electric Company or one of its subsidiaries. Adobe is a trademark of Adobe Systems Incorporated. BCA is a trademark of Thermo Fisher Scientific LLC. Bluetooth is a trademark of Bluetooth SIG. Excel, Microsoft, Windows, and Windows Vista are registered trademarks of Microsoft Corporation. GeneQuant, Novaspec, and Ultrospec are trademarks of Biochrom Ltd. All other third party trademarks are the property of their respective owners. © 2009 -2014 General Electric Company—All right reserved. First published Dec. 2009. GE Healthcare Bio-Sciences AB, Björkgatan 30, 751 84 Uppsala, Sweden GE Healthcare Europe, GmbH, Munzinger Strasse 5, D-79111 Freiburg, Germany GE Healthcare Bio-Sciences Corp., 800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327 USA GE Healthcare Japan Corporation, Sanken Bldg., 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073 Japan 28-9271-31 AD 08/2014
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