january 2011 #18 le gouessant aquaculture technical newsletter › What is an organic fish ? › CO2, how to efficiently degas ? Organic trout farm in open sea water – Ireland This new INF’EAU Issue is willing to evaluate the situation on two quite different subjects. Organic aquaculture is expending rapidly in Europe. This article lows down on the differences between the previous French standard and this new European regulation in force since the 1st of July, 2010. The CO2 subject in aquaculture is sometimes a neglected constraint by fish farmers. Consequences, in particular in hatcheries, yet can be tragic. Removing CO2 with simple techniques is possible, with the exception Defined since the 1920’s, organic farming is on a world scale set for almost 40 years now (International Federation of Organic Agriculture Movements - IFOAM)(1). The French organic agency (Agence Bio), in charge of the development and the promotion of organic farming in France, defines it as follows “organic farming is a specific way of agricultural production, that is to say a set of farming practices being respectful towards ecological balance and the autonomy of farmers. Aiming at soil and natural resources conservation, environment protection and the maintaining of farmers, organic farming is often considered as a the spark of sustainable agriculture”. Organic farming involves applying to a standard. On a general point of view, France lags behind its European neighbours with an utilized agricultural area devoted (1) that the water and fish farm parameters are well set at the beginning. 2010 will have been a year of transition in an difficult economical context : confirmation on structural tensions on raw material prices, leading to a price explosion. We have now to learn to live with this situation and keep on developing new and innovative feeds on both economical and performance aspects. Products will evolve as well to face another major issue : the hardening of the environmental regulation. That will be one of our goals for 2011. Happy new year ! Dominique CORLAY, Aquaculture Manager to organic farming of around 2 %, versus about 6 % for Germany and Spain, 9 % in Czech Republic and Italy and more than 13 % in Austria (2007 figures – source : Agence Bio). As a reminder, the Grenelle Environment Round Table in 2007 set the objective to reach in France 6 % in 2012 and 20 % in 2020. Finally, the global value for organic food products sales has doubled in France between 2005 and 2009 from 1,5 billion to 3 billions Euros, while the demand for conventional food was only growing by 3,6 % according to Agence Bio. Hence comes out a very favourable tendency to organic farming development in Europe. Aquaculture can as well follow that path. In order to get a clearer view, thanks to the help of CIPA (French InterProfessional Committee for Aquaculture Products), we have decided to make a point on the latest changes of organic fish farming standards. summary what is an organic fish ? Development ot the organic fish-farming requirements The requirements of the new european standard Labelling of the products Practical details for an organic conversion Conclusion Key figures p.1 p.2 p.2 p.3 p.3 p.3 p.3 Degassing columns : stripping CO2 efficiently Basic principles of a C02 degassing column Natural vs Artifial aeration Additional remarks p.4 p.5 p.6 p.7 http://fr.wikipedia.org/wiki/Agriculture_biologique#Dans_l.27Union_europ.C3.A9enne What is an organic fish ? Dominique Corlay, Le Gouessant Aquaculture manager | Marine Levadoux, CIPA | Sylvain Delord, Le Gouessant Aquaculture о Organic fish farming was, until the 5th of august 2009, governed in France by CC REPAB F, standard of compliance set in 2000 by the French Agriculture Depar tment, fish -f armers, cer tif ying agencies and professionals of the organic farming sector. At that time, no European standard was set and were coexisting several private, regional or national standards in various countries of the European Union. 2 LE GOUESSANT, commitment for a sustainable fish farming Development ot the organic fishfarming requirements › Articificial polyploïd animals ; › Hormones or hormones derivatives. The regulatory framework of the entire organic agriculture has recently changed with the release of several regulations aiming at homogenizing the requirements of organic agriculture within the EU and in the third countries exporting organic products in the EU (in force since the 1st of july, 2010) : The following requirements apply : The maximum percentage of nonorganic aquaculture juveniles introduced to the farm shall be 80 % by 31 December 2011, 50 % by 31 December 2013 and 0 % by 31 December 2015, For on-growing purposes and when organic aquaculture juvenile animals are not available non-organic aquaculture juveniles may be brought into a holding. At least the latter 2/3 of the duration of the production cycle shall be managed under organic management. Regulation (EC) n°834/2007 : It contains the the general Framework regulation for principles enforceable to organic agriculture organic agriculture, and then to aquaculture. Regulation (EC) n°889/2008 : Implementing regulation (production, processing, labelling, control) completed by REC n°710/2009 for fish-farming, shellfish and seaweed (excluding spirulina) and REU n°271/2010 on labelling It contains the requirements addressing all productions covered by the previous regulation, particularly aquaculture. CCF BIO French Organic Standard (decree of the 5/01/2010) replacing the CCREPAB-F for animal production not covered by the REC n°889/2008 (rabbit, snail, ostrich, aquaculture during the derogation period) and It is left to member states to decide how to apply the provisions. Organic producers under the french standard to the date of publication of the european concerning organic aquaculture (5th of august 2009) have the choice between : > applying the new regulation now, > or keeping on applying the French standard (CC REPAB F being now CCF BIO) until the 30th of June 2013. Furthermore, it is left to member states to decide how to apply the provisions. They have been defined within the INAO and inserted in the CCF BIO. Some of them concern aquaculture. Those texts are available on INAO’s website : www.inao.gouv.fr. The requirements of the new european standard о Simultaneous production > Both organic and non-organic production may be permitted on the same holding during the conversion period (knowing that it shall not exceed the rotation of the batches already on farm. In a raceway farm, organic batches are kept up-stream non-organic ones.) and in hatcheries and nurseries (with a clear physical separation). > Organic and non-organic facilities land-based and/or in fresh water, have to be separated by a minimal distance of 3 km along the river, if the organic farm is located down-stream, and of 1 km bird’s-eye. For sea water ongrowing, a minimal distance of 5 km (or less on the basis of a study) has to be respected between organic and non-organic farms in order to guaranty the absence of water exchanges. о Conversion of a farm о Environmental practises The requirement to follow the production authorization’s order is completed by the obligation to treat the water discharge for inland facilities. > Farm i n g co n d i t i o ns о Caracteristics of the farming facilities Closed recirculation aquaculture animal production facilities are prohibited, with the exception of hatcheries and nurseries. Artificial heating or cooling of water shall only be permitted in hatcheries and nurseries. Natural borehole water may be used to heat or cool water at all stages of production. Aeration is permitted to ensure animal welfare and health, under the condition that mechanical aerators are preferably powered by renewable energy sources. The use of oxygen is only permitted for uses linked to animal health requirements and critical periods of production and transport, in the following cases : › an exceptional case of temperature rise or drop in atmospheric pressure or accidental pollution, › occasional stock management procedures such as sampling and sorting, › in order to assure the survival of the farm stock. о Stocking density The stocking density is calculated on the biological production unit of the entire tanks or cages used. Type of holding Conversion period This part is a synthesis of the above texts but cannot be considered as exhaustive. For further details, it is advised to have a look to those texts. > Production framework о Origin of the animals The main principles of organic agriculture prohibit the use of : › Genetically Modified Organisms (GMO) ; For facilities that cannot be drai- 24 months ned, cleaned and disinfected (lake, permanent pond…) For facilities that have been drai- 12 months after the last ned, or fallowed (earthen ponds, drainage or fallowing drainable plonds…) For facilities that have been 6 months after the last drained, cleaned and disinfected drainage, cleaning and (concrete tanks, resin tanks…) disinfection. For open water facilities including 3 months those farming bivalve molluscs (cage farms in open water or shellfish farming) Species or type of species Maximum stocking density Freshwater salmonids Salmon :20 kg/m3 Brown trout and rainbow trout : 25 kg/m3 Arctic charr : 20 kg/m3 Other salmonids : 15 kg/m3 Sea water salmonids 10 kg/m3 in cages Cod and other Gadidae, sea 15 kg/m3 bass, sea bream, meagre, red porgy, red drum, spinefeet Turbot 25 kg/m2 Freshwater sturgeons 30 kg/m3 3 Inf’eau 18 - january 2011 > Feeds Feeds for carnivorous aquaculture animals shall be sourced with the following priorities : › organic feed products of aquaculture origin ; › fish meal and fish oil from organic aquaculture trimmings ; › fish meal and fish oil and ingredients of fish origin derived from trimmings of fish already caught for human consumption in sustainable fisheries ; › organic feed materials of plant origin - 60 % max of the ration - and of animal origin. If feed mentioned above is not available, fish meal and fish oil from non-organic aquaculture trimmings, or trimmings of fish caught for human consumption may be used for a transitional period until the 31 December 2014. Such feed material shall not exceed 30 % of the daily ration. о Disease prevention and veterinary treatment The use of allopathic treatments, with the exception of antiparasitic products, is limited to : two courses of treatment per year, with the exception of vaccinations and compulsory eradication scheme, for animal living more than a year, one course of treatment per year, for animals living less than a year. The use of parasite treatments, not including compulsory control schemes operated by Member States, shall be limited to : two courses of treatment per year for animals living more than 18 months, only one course of treatment per year for fish living less than 18 months. If the mentioned limits for allopathic treatments are exceeded the concerned aquaculture animals can not be sold as organic products. The withdrawal period for allopathic veterinary treatments and parasite treatments, including treatments under compulsory control and eradication schemes shall be twice the legal withdrawal period compared to the law in force or in a case in which this period is not specified, 48 hours. Vaccination is authorized. The list of substances for use in the presence of aquaculture animal is limited to limestone for pH control. > Majors changes compared to CC REPAB F The European standard has significant differences compared to CC REPAB F. Here below are listed of a few them that have to be highlighted : › While CC REPAB F defined some water quality specifications for the water inlet of organic farms, the European standard does not impose such constraints. › The European regulation does not impose any production tonnage limit per year. › The use of triploïde fish is prohibited while it was allowed in the CC REPAB F (on condition that no use mono-sex females shall be done). › Almost all external treatment substances are now prohibited. Labelling of the products A few cases have to be distinguished : Practical details for an organic conversion It is necessary to closely read through the different texts concerning organic production in order to see if the requirements needed can fit with the facility. The organic certification imposes the commitment to respecting the organic standard but as well to respect a control plan carried out by a certifying agency that delivers the certificate. Conclusion Having done this run down through the new EU organic standard, it appears that some of the restraints to the development of organic aquaculture have been removed (water quality and tonnage limit as an example) – yet, the part on external treatments remains quite problematic. This new standard combined to the fact that the organic sector has the wind in its sails, organic aquaculture has to spark interest. The conversion of some facilities to organic production would probably revitalize the aquaculture sector in some countries. Regulation or standard applied between the 8 August 2009 and the 30 June 2010 Producer under CCF BIO CCF BIO bio before the 8 August 2009 Regulation or standard applied from the 1 July 2010 CCF BIO (with the information of the Auditing Body) RCE 710/2009 RCE 710/2009 Producer converting to RCE 710/2009 organic farmin after the 8 August 2009 RCE 710/2009 Key figures (Source : AgenceBio) : › 126 organic aquaculture facilities within Europe (225 in the world) in 2008. › Around 50 000 tonnes of organic products in Europe in 2008 › Ireland is the world leader for organic salmon production – 8000 tonnes in 2008 › France has 29 organic fish-farms (2009). Le Gouessant Aquaculture’s organic feeds : sea bass, sea bream, trout, salmon, sturgeon and shrimp. See page 8 ! 4 LE GOUESSANT, commitment for a sustainable fish farming Degassing columns : stripping CO2 efficiently In a fish farm, the CO2 concentration in the water can vary according to the water source. Ground water may have CO2 in excess resulting from a stay at a higher temperature/pressure in a particular rock bed (like chalk for instance) or from a particularly high biological activity while filtered through a rock bed. Surface waters generally have a more equilibrated carbonic gas content even though rivers or channels having seasonal vegetation developments may have a considerable fluctuation in their carbonic gas content. In open sea water, it is unlikely to find high carbonic gas content except at night when heavy algal blooms occur. In fish holding facilities, the CO2 content is also related to the fish stock and all organisms present in the tank (aquatic plants, algae, bacteria…) as respiration produces CO2 and consumes O2. Here below are the rough concentrations in fresh water of the main air gases in equilibrium with air (Patm = 760 mm Hg, T=14°C) : Nitrogen = 17 mg/l Oxygen = 10 mg/l CO2 = 0,70 mg/l For precise values according to salinity and temperature see tables here below Table 1 : Solubility of Nitrogen (mg/l) in water at different temperatures and salinities from moist air with pressure of 760 mmHg (Colt, 1984). T (°C) 1 Salinity (‰) 0 5 10 15 20 25 30 35 40 0 23.04 22.19 21.38 20.60 19.85 19.12 18.42 17.75 17 5 20.33 19.61 18.92 18.26 17.61 16.99 16.40 15.82 15.26 10 18.14 17.53 16.93 16.36 15.81 15.27 14.75 14.25 13.77 15 16.36 15.82 15.31 14.81 14.32 13.86 13.40 12.97 12.54 20 14.88 14.41 13.96 13.52 13.09 12.68 12.28 11.89 11.52 25 13.64 13.22 12.82 12.43 12.05 11.69 11.33 10.99 10.65 30 12.58 12.21 11.85 11.50 11.17 10.84 10.52 10.21 9.91 Table 2 : Solubility of Carbon dioxide (mg/l) in water at different temperatures and salinities from moist air with pressure of 760 mmHg (Colt, 1984). T (°C) Salinity (‰) 0 5 10 15 20 25 30 35 40 0 1.09 1.06 1.03 1 0.98 0.95 0.93 0.90 0.88 5 0.89 0.87 0.85 0.83 0.81 0.79 0.77 0.75 0.73 10 0.75 0.73 0.71 0.69 0.68 0.66 0.64 0.63 0.61 15 0.63 0.62 0.60 0.59 0.57 0.56 0.54 0.53 0.52 20 0.54 0.53 0.51 0.50 0.49 0.48 0.47 0.46 0.45 25 0.46 0.45 0.44 0.43 0.42 0.41 0.41 0.40 0.39 30 0.40 0.39 0.39 0.38 0.37 0.36 0.35 0.35 0.34 Source : C.E. Boyd, Water Quality in Ponds for Aquaculture, 1990. These concentrations reflect a situation where the gases are in “normal proportion” in the surrounding atmosphere, i.e. the following rounded up figures Nitrogen: 79 %; Oxygen 21 %. Carbonic gas 0,3 %. If these usual proportions in the surrounding atmosphere change, then the solubility of the different gases in water will change accordingly. Each gas exerts a partial pressure in function of its concentration in the atmosphere; the total sum makes the atmospheric pressure or the total gas pressure as being measured with so called “saturometer” or “total gas pressure meter”(1). (1) Biological and/or physical changes in/around the water body may drastically change the water content of the different gases. One gas can then be temporarily in “supersaturation”, meaning the quantity of gas dissolved in the water exceeds the normal quantity that should be dissolved under the exact circumstances. It can also be “undersaturated” or “desaturated” according to specific situations. It can be roughly estimated that each mole of oxygen consumed by the respiratory activity will generate a mole of carbonic gas. This means in a rough approach that every gram of oxygen La sursaturation des gaz dissous, un phénomène souvent mal connu en aquaculture, Hussenot, J. ; Leclercq, D. Aquarevue N°11 Février-Mars 1987, pp27-30. Inf’eau 18 - january 2011 consumed will generate around 1,375 gram of CO2. CO2 elimination off the fish body is a passive process depending on the concentration gradient between inside and outside the barrier formed by the fish’s gills. It implies that when the CO2 concentration increases in the water, its concentration increases as well it the fish’s blood because being less eliminated by the gills : it is called hypercapnia. This results in the diminution of the blood pH (acidosis), leading to a negative effect on the affinity of the haemoglobin with oxygen and thus on its transport in the fish’s tissues. Moreover, during the filtration of the blood in the kidney, this blood acidosis leads as well to a compensatory reaction in the renal tubules where some mineral deposits of calcium phosphate are formed causing what is called a nephrocalcinosis. The consecutive inflammation of the kidney, of a variable importance, progressively interferes with the renal function and the metabolism of the fish. The lesions weakens it (especially towards diseases) and impairs its growth performances. it causes a drop of the respiratory frequency of the fish (led by the oxygen rate) and therefore diminishes the removing of CO2 from the blood by the gills. So, oxygen is needed but first CO2 has to be degassed with a sufficient aeration in order to avoid this infernal spiral. It is generally accepted that CO2 levels lower than 10 mg/l are well tolerated by fish ; yet, the sensitivity to dissolved gasses varies according to species(2). A concentration of 20 mg/l lowers by 10 % the specific growth rate (SGR) of bass(3) and average to high rates impairs significantly the growth of trout(4). Basic principles of a C02 degassing column The aim of a degassing column is to bring the gas content of water closer to their normal equilibrium at the prevailing physical situation. It will thus strip the gases in excess (nitrogen if any, and carbon dioxide most of the time in aquaculture) and allow for oxygen dissolution up to saturation (or close by). To reach this result, the water flow has to be closely in contact with the ambient air. A maximized contact between air and water is usually obtained by the formation of a thin water film on a plastic media developing an important specific area (m2/m3). A packed column consists of a vertical vessel filled with packing medium or a proper height of self-sustaining plastic media. 1 – The pipe and/or the bulk of structured media has to stand perfectly vertical in order to avoid any flow along the pipe skin itself. Diagram of a basic degassing tower Calcium phosphate mineral deposits A typical case of a nephrocalcinosis on a trout The lower the oxygen concentration, the higher the CO2 toxicity. So the oxygen level in the water has to be monitored. But, conversely, the use in excess of liquid oxygen can make things worse at two levels : it favours high stocking densities, increasing the CO2 level excreted in the water The importance of measuring carbon dioxyde in aquaculture, Pete Southgate, Fish Vet Group, july 2005 Gas control in land based aquaculture, physiological and technical aspects, Blancheton & Al, WAS-2006 (4) Effects of Carbon Dioxide Exposure on Intensively Cultured Rainbow Trout Oncorhynchus mykiss : Physiological Responses and Fillet Attributes, Melody L. Danley & al, Journal of the World Aquaculture Society, Vol. 36 Issue 3 – april 2007. (2) (3) 5 6 LE GOUESSANT, commitment for a sustainable fish farming 2 – The water has to be spread over the media at best to trickle over most of the media. 3 – The column diameter is adapted according to the water flow knowing that the apparent trickling water speed should be within 100-600m/h. In the lower range, there is a risk that the water doesn’t trickle over 100 % of the media. In the upper range, there is a risk of hydraulic clogging of the pipe, stopping any air to flow through with the water. It is therefore recommended to dimension the columns closer to 200-300m/h. PP type Pall-rings Physical properties : > Diameter : 50 mm. > Spécific area : 110 m2/m3. > Apparent density : 65 kg/m3. > Void factor : 93 %. Available from Acui-T. yield is obtained. A number of trials have shown that the higher the column, the better is the result. ! Alternately, structured packing material can be used. They come shaped into cubes or box-like and can be piled above each other. They work better if cross-piled (change the direction at every new level). They are sold under various commercial names. Diameter =2x√[Q/(πxv)] Q = water flow in m3/h V = apparent trickling water speed in m/h Diameter in m 4 – If a “random” type packing is used, the medium size should be 1/8 to 1/10 to the column diameter and should have a large void characteristic (about 85-90 percent). It should be packed in a way that allows the water flow to break up randomly into a thin film that trickles down through the column avoiding short-cuts. Various packing media are sold under the names of rings, barrels, pall rings, spheres and other commercial names (see picture below). Blocks - Each block is 0,9x0,6x0,45m 5 – A dispersion plate should be used on top of the column to optimize the repartition of the water flow. Mostly, a plate with a number of holes (diameter according to the size of the column). Alternately, other options can be taken to break the kinetic energy of the water arriving at the top of the column and disperse it at best over the media upper area. (5) Oxygen and Nitrogen move also faster in/out the water than CO2. Therefore a given column height will not perform the same yield on oxygen and CO2. Regarding CO2 degassing, the column effect might also be impaired by the chemical profile of the water. In a surface sea water loaded with a CO2 excess originating from the fish biomass itself there will be no or little interferences and a good degassing could be obtained with around 0,6 to 1,5 m of head. For the treatment of ground waters, a specific study has to be made as a function of the content of CO2 in excess of an acceptable level. Up to 3 m of head might be required. For columns up to 315 mm water can be delivered on top of the media without a diverting plate, but above this size it is highly recommended to add one. Pall type rings However, there is always an economic optimum according to species and stages being considered. 6 – The height of the columns is the main parameter from which the degassing Note d’Alexander Rose, Fischtechnik International Engineering, GmbH The yield (Y) of a column is expressed as a percentage by the following equation : Y = [1-(FC-CS)/(IC-SC)]x100 Concentrations are expressed in ppm or saturation % FC : final concentration SC: saturation concentration IC : initial concentration For example, if the CO2 content is initially 80 ppm and should be 4 ppm at equilibrium (fresh water from a specific spring) and after passing it through the column, it came down to 20ppm, the yield is : Y = [1-(20-4)/(80-4)]x100 = 79 % Which means that “79 % of the excess of CO2 has been eliminated to atmosphere”. Inf’eau 18 - january 2011 On a pure scientific point of view, the height of a column is determined by the Height of Transfer Unit (HTU) that varies with the medium and the gas according to the following equation(5) : H=HTUxln[(Cl-Ce)/(Cl-Ca)] Height (H) & HTU in m Cl = concentration at saturation of the gas, Ce = concentration of the gas in incoming water, Ca = desired gas concentration For example, at 20°C HTU (CO2) = 0,88 m for a trickling body with the following dimensions : 80x80x2 mm, 75 m2 /m3 and open gap of 94 %. Natural vs Artifial aeration The height of the column creates a chimney effect that strips more efficiently CO2. Without air extractor or ventilator, the chimney effect works due to the height of the column but as well the temperature difference between the air and the water. Most of the time, the degassing columns aiming at stripping CO2 have air blowers (at the bottom of the column) or air extractors (at the top of the column) to set up a constant air counter-flow. To improve the efficiency of a packed column, the air flow has to be above 10 fold the water flow(6). The air-flow can also be used as a lateral cross-flow, which is easier and requires cheaper instrument. An example of lateral air flow obtained with a chicken house ventilator is illustrated on the photo below. it has to be excluded as salt will also add difficulties for the vacuum equipment. To implement the air cross flow from the bottom of the column, a turbine will be installed at a distance, with a protected air intake, and will blow through a properly dimensioned pipe. A water tight join will be established to force air to rise up into the column and escape from the top exit. additional Remarks When CO2 is stripped off fresh water, hard and alkaline waters have a high tendency to deposit lime. Thus it is necessary to clean on a regular basis the degassing tower in order to prevent clogging. To do so, the media can be put in a cylinder made of a chicken wire that can be easily taken down and cleaned in an acid solution. With any sea water, algae might establish on the top of the column if light is available. A well sized and efficient column Ventilated column The ventilator will push air through from a small distance. A bad example To implement an extraction from above, the top of the column has to be tightly closed. The air extraction being made by an horizontal pipe sucking air from the chamber. This method is efficient but the vacuum equipment usually doesn’t last as it sucks very humid air permanently. In sea water, Evaluation of full-scale carbon dioxide stripping columns in a cold water recirculating system, Summerfelt & al, Aquacultural engineering 28, March 2003 (6) Another process often seen is the development of bacterial slime as the media also allows for a biofilm to develop. The shear force expressed by the trickling water is never sufficient to avoid this and slime may build up to clogging the column, breaking its degassing effect. Columns should always be made easily “washable”. Developing an adapted pack column for CO2 degassing is a multi-parameter task and is more difficult than a simple degassing for oxygen recovery. The help of a specialist for the diagnosis and the design should be asked for. Didier Leclercq, Aquaculture engineering | Acui-T contact : [email protected] | +33 (0)6 09 028 724 Antoine Barnaud, Veterinarian doctor | Le Gouessant Aquaculture Sylvain Delord, Agricultural engineer | Le Gouessant Aquaculture 7 january 2011 #18 Le Gouessant aquaculture’s organic feeds › www.agencebio.org › http://agriculture.gouv.fr/l-agriculture-biologique › http://ec.europa.eu/agriculture/organic/home_fr › www.qualite-france.com/page.php?p=4 › www.ecocert.fr/-Tout-sur-la-Bio-et-le-.html › www.fnab.org/ › www.inao.gouv.fr le gouessant aquaculture Société Coopérative d’Intérêt Collectif Agricole à forme civile et capital variable - SIREN 306 957 168 sica du gouessant - z.i. - b.p. 40228 - f-22402 lamballe cedex Tél. +33(0)2 96 30 74 74 - Fax +33(0)2 96 30 74 32 - [email protected] www.aqua.legouessant.fr [email protected] © décember 2010 Places to surf on the web…
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