4/7/2014 OSHA STANDARDS 1 Linda Gylland, MLS (ASCP) April 2014 ASCLS – ND State Meeting Fargo, North Dakota 1) Hazard Communication Standard: CFR 1910.1200 (Right to Know) 2) Hazardous Chemicals in Laboratories: CFR 1910.1450 3) Bloodborne Pathogens Standard: CFR 1910.1030 4) Formaldehyde Standard: 5) Personal Protective Equipment Standard CFR 1910.132 6) Occupational Exposure to TB Standard (CHEMICAL HYGIENE PLAN) 2014 LAB SAFETYABC’S OF DO’S, DON’TS AND WHY’S CM (MINIMIZE EXPOSURE TO BLOOD-BODY FLUIDS/ HIV, HBV, HCV) QLS CFR 1910.1048 (MINIMIZE EXPOSURE/ MONITORING) withdrew in 2003, now is under CDC 7) ( VDT’s- video display terminals, hearing, repetitive motion, lifting, workstations, chairs) Ergonomics recommendations 4 MORE REGULATING AGENCIES OBJECTIVES Upon completion of this presentation, the participant will be able to: 1) Summarize the duties of various regulating agencies and understand the reasoning for enforcement. 2) Review some CAP deficiencies given to labs, checklist questions, and some of the 2013 revisions. 3) Describe the importance of safety compliance and consequences of violations. 4) Correlate examples from this presentation to 2 your workplace situation. EPA: ENVIRONMENTAL PROTECTION AGENCY (REGULATES WASTE DISPOSAL) NRC: NUCLEAR REGULATORY COMMISION (REGULATES RADIATION HANDLING/DISPOSAL) DOT/U.S. POSTAL SERVICE: (REGULATES SHIPPING AND TRANSPORTATION) CLIA 1988: Clinical Laboratory Improvement Amendments (REGULATES CLINICAL LABS) CLSI – Clinical & Laboratory Standards Institute CMS – Centers for Medicare & Medicaid Services US DEPT OF HOMELAND SECURITY: (BIOTERRORISM STANDARDS) 5 OSHA: OCCUPATIONAL SAFETY AND HEALTH MORE REGULATING AGENCIES ADMINISTRATION CDC: HANDLING INFECTIOUS ORGANISMS NIH/NIOSH: NATIONAL INSTITUTE OF HEALTH, NATIONAL INSTITUTE FOR OCCUPATIONAL SAFETY AND HEALTH-DIVISION OF CDC, NFPA: NATIONAL FIRE PROTECTION ASSOC. VOLUNTARY ACCREDITING BODIES: COLA: 1988 – Help labs be compliant with CLIA, approved by CMS, Need a comprehensive safety policy to provide employees with: safety equipment safety training free medical care for job injury WORKPLACE INJURIES/ILLNESSES recognized by JC General Duty Clause: Employers must maintain a safe work environment, free from hazards that could cause death or physical harm to employees…. CAP: COLLEGE OF AMERICAN PATHOLOGISTSBOARD CERTIFIED PATHOLOGISTS AND PRACTICING LABORATORY PROFESSIONALS AS INSPECTORS, receives authority from CMS, recognized by JC. 3 JOINT COMMISSION- PATIENT SAFETY- ISSUES STANDARDS, CONDUCTS SURVEYS AND SITE VISITS 6 1 4/7/2014 CONSEQUENCES OF INSPECTION DEFICIENCIES ACID/BASE STORAGE OSHA- warnings or proposed fines; you can contest the citations, sometimes fines are reduced CAP – you can also contest these with supporting Organic Acid Inorganic Acid Store separately Organic Solvent * Organic Compound Inorganic Compound *LG QUANTITIES IN FLAM CABINET from inorganic acids documentation Acetic Acid (IN SEPARATE CUPBOARD) Acetone Acetonitrile Ammonium acetate powder Formic Acid (IN SEPARATE CUPBOARD) HCl Isopropyl Alcohol 2-Mercaptoethanol Methyl Alcohol Methanol Phosphoric Acid crystals Polyethylene Glycol crystals Sulfuric Acid Zinc Sulfate powder Xylene Phase I Deficiency: submit written statement with corrective action taken Phase II Deficiency: corrective action plus documentation (policies, procedures, supporting records- patient reports, log sheets, purchase orders, photographs, memos or minutes from meetings. Records must demonstrate compliance with policy or procedure. X X X X X X X X X X X X X X X 7 10 BIOHAZARD (SHARPS) ACID/BASE STORAGE Do not overfill - fill 2/3 full Discard proper (sharp) material into sharps, no paper, cardboard…… CAP GEN. 76700 (II) Store in separate cabinets near floor, below eye level, not under sinks 8 ACID/BASE STORAGE 11 BIOHAZARD SIGNAGE Do not store organic acids with inorganic acids WHY? TO PREVENT INTERACTION OF FUMES AND CORROSION OF STORAGE CABINETS Bloodborne Pathogen Std: 1910.1030(d)(4)(iii)(A)(2)(i) (OSHA violation to discard biohazardous waste in regular garbage but not visa versa…..) CAP GEN. 78000 Sharps Disposal (II) 9 CAP Gen 77900 (II) Biohazard Disposal Containers-infectious wastes-Solid tight fitting covers for transport/disposal Symbols on bags: OSHA 1910.1030 (clear bag within facility but biohazard symbol to send out) JC – could cause confusion, use biohazard bags BEST PRACTICE- use biohazard bags with symbols 12 2 4/7/2014 BIOHAZARD SIGNAGE DO NOT DO THIS!!!! OSHA 29 CFR 1910.1030 (Bloodborne Pathogen) Biohazard labels placed on entrance to any area likely to contain blood and OPIM (other potentially infectious materials. Cleaning without gloves Drying the countertop with paper towel after wiping down with the disinfectant wipe DO THIS: Wear gloves during cleaning, leave wet for the required contact time 13 16 BSL3 ROOM CHEMICAL HYGIENE PLAN OSHA 29 CFR 1910.1030 Bloodborne Pathogens and Biosafety OSHA OSHA CAP GEN 76000 (II) CHP – evaluate chemicals for MIC. 18968 Agents of Bioterrorism (II) Antechamber 1910.1450 Chemical Hygiene Standard -Hazardous chemicals in laboratories 1910.1200 Hazard Communication Standard- to inform of potential chemical hazard RT, CA, T; fume hood function verification BSL3 Room 14 CLEANING: CONTINUOUS WET CONTACT TIME Chemical Name Manufacturer Part Number 1-Naphthyl acetate 3-Amino-9-Ethylcarbazol 5-Fluoro-2-Deoxyuridine 5-Bromo-2-Deoxyuridine 5-Sulfosalicylic Acid 25-Hydroxy-Vitamin D2 25-Hydroxy Vitamin D3 25-Hydroxyvitamin D2-(6,19,19-2D3) 25-Hydroxy Vitamin D3(26,26,26,27,27,d5) Acetic Acid, Glacial Acetone Acetonitrile Aerospray Nozzle Cleaning Solution Aerospray Stain Residue Solvent Alcohol Prep Pads (Webcol/Curity) Ammonium Acetate Sigma-Aldrich Sigma-Aldrich Mayo (Sigma) Mayo (Sigma) Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich/Isosciences N8505 A5754 F-0503 B5002 247006 17937 705888 705497 Medical Isotopes Fisher Scientific Thermo Scientific Fisher Scientific Wescor, Inc. Wescor, Inc. Kendall Sigma-Aldrich D2831 BP 2401-500 C4300 C2H3N SS-029 SS-230 6818 A1542 P CO I X X X X X X X X X X X X X X X X X X X X CA F T O RT X X X X X X X X X X X X X X X X X 17 DO NOT…. Sanicloth Plus (red) 3 minutes Bleach (orange) 4 minutes (C difficile) AF3 (grey) 3 minutes (also for TB) Super Sanicloth (purple top) 2-3 minutes Sanicloth HB -Hep B (green/blue) 10 min Hepacide Quat - damp towel with tap water/spray Hep Quat- 10 minutes 10% Bleach : 10 minutes (make fresh daily) READ PRODUCT LABEL FOR SURFACE REQUIREMENTS per Infection Control…… 15 18 3 4/7/2014 EATING IN THE LAB EYE WASH OSHA 1910.151 follows ANSI standard (for hazardous chem. flush 15 min, for blood or OPIM CAP Gen 77400 (II) Within 10 sec travel distance, unobstructed path, hands-free flow once activated, signage, tepid water (7/29/13) Water temperature should be Includes no GUM CHEWING!!! OSHA 1910.1030 (d) (2) ix is “intended primarily to eliminate or minimize indirect transmission of bloodborne pathogens from contaminated environmental surfaces,” according to the OSHA’s CPL 02-02-069 - CPL 22.69 - Enforcement Procedures for the Occupational Exposure to Bloodborne Pathogens 1910.1030 (d) (2) does not literally mention food, the inference is obvious, and chewing gum is recognized as food by the FDA CAP GEN. 74400 (II) no eating 19 EVACUATION PLAN/MAP/INSTRUCTIONS between 60°F and 100°F. Actual temperature recording is not required.) Evidence of Compliance: ✓ Records of testing the plumbed eyewash AND ✓ Records of manufacturer's specifications (7/29/13) Test weekly, run 3 min to flush out organisms (Pseud sp,Mycobacteria; tepid water (60-95 F) 100 ft/ 10 seconds 22 EYE WASH OSHA 1910.36 (exiting-prompt escape in emergency) 29 CFR 1910.37 CAP Gen 73900 (II) need comprehensive, documented & workable evacuation plan; emergency lighting for safe evacuation; specific instructions for persons with disabilities Routes clearly posted, maps are optional 20 STRYKER EVACUATION CHAIR 23 FANS Joint Commission, OSHA, Patient Safety (for non-ambulatory staff and patients- transport down stairs) Fans will not be used in the lab for everyday temperature control. Facilities department will be contacted in situations to provide room temperature adequate for analyzer operation and staff comfort. A safe working environment is the goal in prohibiting fans near bio-safety cabinets, chemical fume hoods and areas that could generate aerosolization of pathogens, cause evaporation of reagents and specimens or interfere with negative pressure that is required when dealing with infectious agents. CAP Gen. 61300 (I) Climate Control/ OSHA has no requirements 21 CAP has rec’d many calls on this 24 4 4/7/2014 FIRE ESCAPE ROUTES/FIRE EXIT Which ones are used appropriately? FANS CAP Gen. 75300 (II)- room > 1000 ft2, need 2 exit doors, 1 opens into an exit route 2 3 1 25 Width of aisles: not <44 inches (50 people), 44=minimum for business or not <36 inches (<50 people) per fire code 28 4 FIRE DRILLS FIRE ALARM PULL STATION CAP Gen. 75600 (II) – fire alarm station in or near the lab OSHA and NFPA CAP Gen 75400 (II) “conduct periodically, at least annually of all personnel, exit once/year (Evidence of Compliance: Records of participation for ALL employees in fire drills involving laboratory evacuation at least annually: employee roster with dates OSHA 1910. 155-1910-165 CAP inspectors have asked staff for locations Per OSHA, fire alarm pull stations are NOT required in small clinics (need plan in place: RACE, PASS, etc) 26 FIRE EXTINGUISHER FIRE ALARM: OSHA 1910.165, CAP GEN. 75500 (II) Must be audible in all areas of the lab, for hearing impaired-need system in place (strobe lights….) 29 FLAMMABLE CABINET CAP GEN. 75700 (II) – portable fire extinguishers in areas with flammable/combustible liquids, near doors or outside CAP Gen. 75800 (II) – personnel instructed in use NFPA 99, NFPA 45 – clutter free 36” radius OSHA- maximum travel distance not > 75 ft (Class A (paper) or >50 ft (class B-chemical) CAP Gen. 76500 (II) OSHA 1926.152(b)(4)(vii) 1910.106(a)(3) i 27 30 5 4/7/2014 FLAMMABLE CABINET GAS CYLINDER STORAGE OSHA 1910.169-171 CAP GEN 76800 (II) Gas cylinder storage CAP Gen 76900 (II) was (I) Gas cylinders containing flammable gases For storing excess flammables, Need clear means of egress per fire code, clear space in front of cabinet OSHA: maintain 1 clear aisle at least 3 feet wide 31 FLAMMABLE CABINET 34 GLOVES/PHLEBOTOMY CAP Gen 74100 (II)-PPE provision and usage Gen 74200 (II)- PPE instruction Gen 76300 (II)- PPE and hazardous materials Gen 77700 (II)- was (I) – latex free OSHA 1910.138, 1910.1030 CAP GEN 76500 (II) Flammable liquid storage outside of cabinet: can have 1 gallon of class I, II and IIIA liquids/100 ft2 and doubled if sprinkler system; OSHA is same 32 FLASHPOINT The minimum temperature at which a liquid vaporizes to produce an ignitable mixture with air FP <100oF (38oC) =Flammables, Class I Solvents FP 100oF (38oC)- 140oF (60oC)= Combustibles, Class II Solvents 35 NO NO HAND HYGIENE CAP Gen. 74200 (II) decontamination of hands after glove removal OSHA 1910.1030 Bloodborne Pathogen Standard (Ethyl ether, Acetone, Ethyl alcohol, Xylene, Methanol) (Butyl ether, Toluidine, Acetic anhydride) 15 -30 seconds at the sink Use foam when hands are not visibly soiled Most dangerous solvents – lowest flash points 33 FINGERNAILS SHOULD BE NO LONGER THAN ¼ INCH BEYOND END OF FINGER! Artificial nails >>safety risks, (infection control issue, patient safety) “CDC 2002 Guideline For Hand Hygiene in Healthcare Settings” 36 6 4/7/2014 HAZARDOUS MATERIALS EMERGENCY TREATMENT JEWELRY CAP GEN 76400 (II) explicit instructions posted for emergency treatment of chemical splashes/injuries Conduct electricity, Get caught in equipment USE COMMON SENSE! 37 Can puncture gloves 40 KILL THOSE BACTERIA! LABORATORY CHEMICAL SAFETY ROUTES OF EXPOSURE: RESPIRATORY – Inhalation hazards from chemicals that produce dust, fumes, gases or vapors require use of face masks, safety glasses or goggles, face shields, chemical fume hoods or biosafety cabinets. OCULAR – Wear safety glasses, goggles or use a face shield to protect your eyes from chemical splashes. ORAL – Wear a surgical mask or face shield to keep chemical splashes away from your mouth. No mouth pipetting is allowed – use mechanical pipetting devices only. Wear gloves, wash hands regularly and don’t put any objects including fingers into your mouth. CONTACT - To avoid contact with chemicals, guard exposed skin and mucous membranes with protective clothing and equipment (lab coats, gloves, face shields, face masks, safety glasses or goggles, fume hoods or biosafety cabinets. Keep lab surfaces clean. When performing procedures that create droplets or possible spills, clean immediately. IN CASE OF A CHEMICAL SPLASH OR SPILL RESULTING IN INJURY REQUIRING EMERGENCY TREATMENT: IMMEDIATELY WASH AFFECTED AREA WITH A GENEROUS AMOUNT OF WATER. ON YOURSELF OR YOUR CLOTHING: SHOWER (PULL THE RING ON THE SAFETY SHOWER FOR RAPID DISCHARGE OF WATER) AND REMOVE CONTAMINATED CLOTHING. SEEK MEDICAL ATTENTION IF NECESSARY. IN YOUR EYES: WASH EYES USING THE EYE WASH STATION FOR 15 MINUTES. IF AN EYE WASH STATION IS NOT AVAILABLE, FLUSH WITH SINGLE-USE PERSONAL EYE WASH SALINE BOTTLES AND SEEK IMMEDIATE MEDICAL ATTENTION. ON THE FLOOR: CHECK THE MSDS FOR APPROPRIATE ACTION TO BE TAKEN. IN CASE OF MINOR SPILLS, USE THE SPILL KIT IN YOUR LABORATORY OR ABSORB THE SPILL WITH PAPER TOWELS, COVER WITH 10% BLEACH, ABSORB FOR 10 MINUTES AND DISPOSE MATERIAL INTO BIOHAZARD BAG OR CONTAINER. REPORT ALL MAJOR SPILLS TO THE SAFETY OFFICER AND COMPLETE A LABORATORY SPILLS REPORT FORM Contact time (dwell time) for disinfectants: Sani-cloth Disposable Cloths: Purple top (Super Sanicloth) 2-3 minutes Orange top (bleach) 4 minutes Grey top (AF3) 3 minutes Red top(Sanicloth Plus) 3 minutes Sanicloth HB 10 minutes Keep entire surface wet for entire disinfection contact time per label instructions to achieve the level of KILL required! 38 INFECTION CONTROL 41 LAB COATS OSHA 1910.1030 and ASMT (American Society for Testing & Materials) CAP GEN 74100 (II) PPE Provision and Usage CAP GEN 74200 (II) PPE Instruction fluid resistant, impervious fronts, full length, long sleeves, knitted cuff, closed in front, acceptable spray rating, break test, suter hydrostatic pressure test, air permeability Wear at ALL times in the lab; remove when leaving the lab 39 42 NO NO 7 4/7/2014 LAB COATS SEPARATION OF CLEAN & DIRTY NOISE MONITORING OSHA 1910.95 (Occupational Noise Standard or Hearing Conservation Standard)= safe noise level < 90 dB based on 8 hour TWA (time-weighted-average) CAP GEN. 77300 Excessive Noise (II) was (I) –need to monitor noise exposure if excessive noise indicates >85dB, where people have to shout to be heard…. 43 Infection Control! MOUTH PIPETTING Occupational Health Ear plugs should be available upon request Badge dosimeter Sound level meter 46 ORDER OF DONNING AND DOFFING 20 CFR 1910= no eating, drinking, smoking, gum chewing, applying cosmetics, lip balm or contact lens manipulation in the lab CAP GEN. 74400 (II) HOW TO PROPERLY PUT ON AND REMOVE LAB COAT AND GLOVES: no mouth 1) Put on lab coat or isolation gown (mask, goggles, hat, shoe covers) 2) Put on gloves, cuff of gloves should overlap cuff of lab coat sleeve pipetting; same as OSHA To put on lab coat: -hand in sleeve -lower coat over shoulder -2nd hand in other sleeve -button or snap up lab coat 44 Isolation gown: Fully cover neck to knees, arms to wrists Wrap around the back Fasten with the sticky back end of belt To take off soiled lab coat or isolation gown: -remove gloves (goggles, shoe covers, hat) -unbutton or unsnap coat -lower across both shoulders, starting to turn inside out and pull sleeves out -roll up and put in laundry bag -if soiled with blood, etc, put in biohazard bag and close tightly with tie, put in laundry bag (Remove mask last) 47 Order of “donning” before entering BSL3 room: •Gown •Mask •Goggles •Bouffant •Shoe covers •Gloves MSDS/SDS OSHA Hazard Communication Standard 1910.1200 New HCS/GHS/SDS Dec 1, 2013 CAP GEN. 76100 (II) Current MSDS and CHP, do not need paper copy as long as you have emergency plan for power failure. 45 Order of “doffing” when leaving BSL3 room: •Gloves (remove in BSL3 room) •Goggles •Bouffant •Shoe covers •Gown •Wash hands •Mask •Wash hands 48 8 4/7/2014 ORDER OF DONNING AND DOFFING AN EASY WAY TO REMEMBER: PROPER LAB COAT/SHOES Per OSHA Healthcare Advisor: CAP GEN. 74100 (II), GEN 74200 (II), OSHA 1910.145, OSHA 1910.132 Don PPE from the bottom up: 1. Gown 2. Mask 3. Goggles 4. Gloves (when raised above head) Doff PPE in alphabetical order: 1. Gloves 2. Goggles 3. Gown 4. Mask 52 49 ARMED INTRUDER PLANTS IN THE LAB QUICKLY BUT SAFELY CAP GEN 73800 (II) Disaster Preparedness…….. For BSL2 labs from Biosafety in Microbiological and Biomedical Laboratories: Animals and plants not related to the work being conducted are not permitted in the laboratory. Plants not only provide a breeding ground for many organisms, they are also impossible to regularly disinfect. BSL guidelines standard: no plants in labs! 50 PPE OUTSIDE OF THE LAB? NO NO NO! 53 RESPIRATORY MASK Infection Control issue and OSHA and CAP OSHA 29 CFR 1910.134 (Respiratory Protection Standard) N95 mask- annual fit testing CAP GEN. 74900 (II) TB Exposure Plan Infection Control In areas with infectious agents, on exposure for phlebotomy, use in BSL3 room Rest rooms Classroom Admin Offices OSHA: employees must remove PPE prior to leaving the work area! 51 54 9 4/7/2014 SPACE HEATERS ULTRAVIOLET LIGHT Portable Electric Space Heaters not allowed from home--- electrical shocks, fire….. CAP GEN. 77600 (II) UV Light Exposure- need signage & protection OSHA 1926.154 Fire Protection & Prevention (not >2120F, need management approval Low wattage radiant heat panels allowed per facility management approval 55 58 UNIVERSAL PRECAUTIONS (STANDARD PRECAUTIONS) SPILL CONTROL STATION (SPILL KITS) OSHA 1910.1030 (Bloodborne Pathogens) CAP GEN. 74600 (II) Spill Handling/documentation CAP GEN. 76400 (II) Hazardous Material Emergency Treatment control of spills - assess usability periodically CAP MIC.18985 Spill Handling/contaminated materials CAP GEN. 74000 (II) Bloodborne Pathogens Universal Precautions- older CDC term (1986-88) BB pathogen –for fluids likely to have blood or visibly bloody: blood, CSF, pleural, amniotic fluid; not urine, vomit, feces or sputum Standard Precautions- replaced Universal Precautions in 1996for all body substances except sweat. 56 TRANSPORTATION OF FLAMMABLES 59 VACCINES CAP GEN. 77800 (II) Hazardous Chemical Waste Disposal, local regulations for transport of flammables…..need DOT approved box for intown transport of flammables (spent ClearRite….) NO YES 57 CAP GEN. 74700 (II) Hepatitis B Vaccinations CAP GEN. 74800 (II) Viral Exposure-HIV,HBV, HCV OSHA 1910.1040 -free vaccine made available 10 working days after training completed. Mandatory influenza vaccinations to achieve herd immunity (when the vaccination of a significant portion of a population (or herd) provides a measure of protection for individuals who have not developed immunity)…….goal 90% (100%??????) Per CDC, overall rate of 72% (2012-2013) 66.9% 2011-2012) 60 10 4/7/2014 Y AND Z…. WASTE DISPOSAL (HAZARDOUS WASTE) CAP GEN.77800 (II) Hazardous Chemical Waste Disposal OSHA 1910.1200 Hazard Communication Standard Policies, documentation, compliance, EPA, local & state regulations FOR YOU: 61 XYLENE (AND FORMALDEHYDE MONITORING 64 AVOID SAFETY AUTO PILOT CAP GEN. 76000 (II) Chemical Hygiene Plan OSHA 1910.1048 Badge monitoring vapors Exposure monitoring when permissible exposure limits (PELs) are exceeded Xylene: 8 hrs=100PPM, 15 min = 150 PPM, Formaldehyde: 8 hrs=0.50 PPM, 15 min=2 PPM 62 CAP REVISED 7/29/13 CHECKLIST REQUIREMENTS 65 REMEMBER….. SAFETY NEVER TAKES A GEN. 61350 (II changed to I)- Direct sunlight GEN. 61400 (II changed to I)- Hallway obstructions GEN. 74000 (II) some content changed- BB path GEN. 75900 (I changed to II)- Electrical grounding GEN. 76900 (I changed to II)- Gas cylinders GEN. 77300 (I changed to II)- Excessive noise GEN. 77400 (II) some content changed- Eyewash GEN. 77500 (I changed to II)- Liquid Nitrogen GEN. 77700 (I changed to II)- Latex allergy 63 66 11 4/7/2014 REFERENCES http://www.flickr.com/photos/11152812@N07/1056033556/ http://www.amazon.com/Barco http://www.glogster.com/pumpkinpie59/no-eating-in-the-lab/g6m4ba2rnfvpbk8eo2v9mua0 http://cenblog.org/the-safety-zone/2010/09/safe-science-garblab-coats/ http://www.cmu.edu/ehs/newsletters/lab-safety/Proper-LabShoes.html http://www.thebudgetfashionista.com/archive/how-to-wearcocktail-rings/ http://www.consumerreports.org/cro/best-space-heaters.htm http://www.apartmenttherapy.com/dimplex-mini-cube-electric67 sto-139647 REFERENCES http://blogs.plos.org/obesitypanacea/2012/01/30/doeschewing-gum-help-with-weight-loss/ https://webshop1.diagnostics.siemens.com/edbna2/ebusin ess/USWebshop/homeGuest.jsp http://www.drybabe.com/blog/menopause-hot-flash-or-hotflush/ http://ehs.unc.edu/training/self_study www.denlineuniforms.com/protection-plus http://www.cdc.gov/flu/healthcareworkers.htm http://www.kemmed.com/toxic%20vapor%20monitors/moni tors.html 68 http://en.wikipedia.org/wiki/Herd_immunity 12
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