PRIONICS AG WAGISTRASSE 27 A PHONE +41 44 200 20 00 8952 SCHLIEREN-ZURICH FAX +41 44 200 20 10WWW.PRIONICS.COM [email protected] SWITZERLAND Versatile Sample Collection Tool. GENOTUBE LIVESTOCK ■■ Easy to Use – simple non-invasive tool for the collection of diagnostic samples ■■ Reliable – fast drying ensures excellent sample quality at the lab ■■ Cost Efficient – no refrigeration or rapid transport required ■■ Versatile – different sample matrices can be collected and stored WWW.FORENSIX.CH forensiX GenoTube Livestock VETERINARY DIAGNOSTICS GENOTYPING APPLICATIONS The GenoTube with its proprietary drying system offers new possibilities for the easy collection of animal diagnostic samples. Due to fast drying the collected sample is conserved and guarantees that the sample arrives in the best quality at the lab. Once dried, collected samples can be transported and stored without refrigeration, which makes transportation simple and very cost effective. In the lab the samples can be easily reconstituted and used for numerous analyses. Genetic-based breeding schemes are being used increasingly by livestock farmers, etc. Obtaining high yields of intact genomic DNA is gaining importance as genotyping and sequencing of DNA samples become standard practice. The processes of collection, transport, storage and geno typing of samples must therefore be integrated, robust, and cost-effective. Prionics has devised a simple method for sample collection: The Prionics GenoTube swab. Saliva / Nasal Swabs The non-invasive collection of a saliva sample or a nasal swab sample with the GenoTube is easy and straightforward. The samples can be analyzed in the lab for the presence of antigens or antibodies by PCR or ELISA methods, depending on the agent of interest. Research has shown that animal oral fluids can be used to detect infections of PRRSV, PCV2, SIV, M. hyopneumoniae, etc. The GenoTube also allows oral fluid sampling of individual animals and therefore enables the diagnosis of individual animals. Blood Although saliva might be preferred due to its ease of collection, blood may also be taken with minimal invasion. By scarifying an ear a small amount of blood can be drawn. The blood sample does not have to be refrigerated for transportation and storage due to the drying properties of the GenoTube. The dried blood can then be reconstituted and analyzed for the presence of antigen or antibody by either PCR or ELISA methods. Feces Common methods to collect and store fecal samples often lead to the samples becoming overgrown with fungi and bacteria. The GenoTube offers a very easy and convenient way to collect feces samples and to halt fungal and bacterial growth. The sample can be analyzed for antigen with PCR and ELISA in the lab in the same condition it has been collected on the farm. EASY HANDLING 1 2 3 Collecting animal tissue samples using the GenoTube is easy and rapid. After the swab containing the sample is placed back in the tube, the sample is dried and the DNA in the sample conserved. The tube can be transported and stored under normal conditions without refrigeration. DNA contained in the sample remains stable for years. The amount of nasal tissue DNA collected using the GenoTube is sufficient for testing with the Illumina chip tech nology and results are as good as blood samples. BENEFITS ■■ non-invasive ■■ excellent sample quality at the lab – ensured by the fast drying of the sample ■■ versatile – different sample matrices can be collected ■■ Sample can be used for antigen or antibody diagnostics; e. g. PCR, ELISA, etc. ■■ Low transportation costs – refrigeration or rapid transport not required ■■ Clear identification and traceability – identical ID-Number on both the swab cap and the tube ■■ Conservation – no degradation of DNA after long term storage ■■ Independent of environmental condition – drying not influenced by local humidity Drying Performance 100 % 90 % 80 % Residual Moisture / % A variety of different sample matrices can be taken with the GenoTube Livestock: nasal swabs, saliva, blood and feces samples 70 % 60 % 50 % 40 % 30 % 20 % 10 % 0% 0 2 4 6 8 10 12 14 16 18 20 22 24 LF2-1308 time/h 5 sec forensiX GenoTube CONTACT INFORMATION GenoTube Livestock | Art. Nr. 9062010 PRIONICS AG WAGISTRASSE 27 A CH-8952 SCHLIEREN-ZURICH SWITZERLAND PHONE +41 44 200 20 00 FAX +41 44 200 20 10 [email protected] WWW.PRIONICS.COM
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