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foodproof® Sample Preparation Kits
Catalog Numbers
foodproof Sample Preparation Kit I S 400 04
foodproof Sample Preparation Kit II S 400 05
foodproof GMO Sample Preparation Kit S 400 06
The foodproof Sample Preparation Kits are optimized for isolation
of bacterial DNA from enrichment cultures of various food samples
or plant DNA, even those with a high potential for PCR inhibition
(raw material and processed food).
Background Information
All foodproof Sample Preparation Kits generate highly purified DNA, even from matrices with a high potential for
PCR inhibition. The quality of the DNA obtained with the kits is suitable for applications using any PCR system.
Analytical Procedure
Step
Description
Sample Prep I
Sample Prep II
GMO Sample Prep
1
DNA is extracted by incubation with the
Extraction Buffer.
x
x
x
2
Proteinase K digestion of endogenous
nucleases and other impurities.
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x
3
DNA is bound to the glass fibers
pre-packed in the High Pure filter tube.
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4
Washing of bound DNA, and purification from
salts, proteins, and other cellular impurities.
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5
Purified DNA is recovered using the Elution Buffer.
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x
Features
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Allow 100 isolations
Purified DNA is free of other cellular components and DNA polymerase inhibitors
Avoiding organic solvent extraction and DNA precipitation
Rapid, reliable and suitable for all food samples; even those with a high potential
for PCR inhibition (finished products and raw materials)
Easy to use
Time to result ‚ 60 - 90 min
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foodproof Sample Preparation Kit I
Catalog No. S 400 04
The foodproof Sample Preparation Kit I is optimized for isolation of bacterial DNA from enrichment cultures of various
food samples (raw material and processed food). For preparation of DNA from Salmonella, E. coli O157, E. sakazakii
and other Enterobacteriaceae.
Test Principle
Step 1 Following sample homogenization, the DNA is extracted at high temperature with the extraction buffer provided.
After centrifugation, the DNA is bound selectively to special glass fibers pre-packed in the filter tube.
Step 2 In step 2 the DNA is purified in two “wash-and-spin” steps to remove potential PCR inhibitors, then a low-salt elution releases the DNA from the glass fiber. This simple method eliminates the need for organic-solvent extractions
and DNA precipitation, thus providing rapid, simultaneous purification of many samples.
foodproof Sample Preparation Kit II
Catalog No. S 400 05
The foodproof Sample Preparation Kit II is optimized for isolation of bacterial DNA from enrichment cultures of various
food samples (raw material and processed food). For preparation of DNA from Listeria monocytogenes, Listeria Genus,
Campylobacter and beer spoilage bacteria.
Test Principle
Step 1 Following sample homogenization, the DNA is extracted at high temperature with the extraction buffer provided.
After centrifugation and Proteinase K digestion, the DNA is bound selectively to special glass fibers pre-packed in
the filter tube.
Step 2 In step 2 the DNA is purified in two “wash-and-spin” steps to remove potential PCR inhibitors, then a low-salt elution releases the DNA from the glass fiber. This simple method eliminates the need for organic-solvent extractions
and DNA precipitation, thus providing rapid, simultaneous purification of many samples.
foodproof GMO Sample Preparation Kit
Catalog No. S 400 06
The foodproof GMO Sample Preparation Kit is optimized for the isolation of DNA from various food samples of plant
origin (raw material and processed food).
Test Principle
Step 1 Following concentration by centrifugation, the cells are lyzed during a short incubation with the foodproof
Sample Preparation Kits Lysis Buffer provided. After centrifugation the DNA is bound selectively to special glass
fibers pre-packed in the filter tube.
Step 2 In step 2 the DNA is purified in two “wash-and-spin” steps to remove potential PCR inhibitors, then a low-salt elution releases the DNA from the glass fiber. This simple method eliminates the need for organic-solvent extractions
and DNA precipitation, thus providing rapid, simultaneous purification of many samples.
foodproof® is a registered trademark of BIOTECON Diagnostics.
BCD_PS_S40004_V01/08
www.bc-diagnostics.com
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