1. science
2. medicine
3. genetics

PUBLICATIONS
Spitz, M.R., Gorlov, I.P., Amos, C.I., Dong, Q., Chen, W.,
Etzel, C.J., Gorlova, O.Y., Chang, D.W., Pu, X., Zhang,
D., Wang, L., Cunningham, J.M., Yang, P., and Wu, X.
August 25, 2011. Variants in Inflammation Genes Are
Implicated in Risk of Lung Cancer in Never Smokers
Exposed to Second-hand Smoke. Cancer Discovery
Paez, D., Pare, L., Espinosa, I., Salazar, J., Del Rio, E.,
Barnadas, A., Marcuello, E. and Baiget, M. May 18, 2010.
Immunoglobulin G fragment C receptor polymorphisms
and KRAS mutations: Are they useful biomarkers of
clinical outcome in advanced colorectal cancer treated
with anti-EGFR-based therapy? Cancer Science
Chu, X. Song, H.D., et al. August 14, 2011. A genomewide association study identifies two new risk loci for
Graves’ disease. Nature Genetics
Sagreiya, H., Berube, C., Wen, A., Ramakrishnan, R., Mir,
A., Hamilton, A., Altman, R.B. May 3, 2010. Extending
and evaluating a warfarin dosing algorithm that
includes CYP4F2 and pooled rare variants of CYP2C9.
Pharmacogenetics and Genomics
K. Shitara, S. Ito, K. Misawa, Y. Ito, H. Ito, S. Hosono,
M. Watanabe, K. Tajima, H. Tanaka, K. Muro, and K.
Matsuo. June 20, 2011. Genetic polymorphism of IGF-I
predicts recurrence in patients with gastric cancer
who have undergone curative gastrectomy. Annals of
Oncology
Chan, M., Chan, M.W., Loh, T.W., Law, H.Y., Yoon, C.S.,
Than, S.S., Chua, J.M., Wong, C.Y.,Yong, W.S., Yap, Y.S.,
Ho, G.H., Ang, P, and Lee, A.S.G. May 2011. Evaluation
of Nanofluidics Technology for High-Throughput SNP
Genotyping in a Clinical Setting. Journal of Molecular
Diagnostics
P. J. Maughan, S. M. Smith, D. J. Fairbanks, and E. N.
Jellen. March 16, 2011. Development, characterization
and linkage mapping of single nucleotide polymorphisms
in the gram Amaranths. The Plant Genome
Habicht, C., Seeb, L. W., Myers, K. W., Farley, E.V.,
Seeb, J. E. 2010. Summer-Fall Distribution of Stocks of
Immature Sockeye Salmon in the Bering Sea as Revealed
by Single-Nucleotide Polymorphisms. Transactions of
the American Fisheries Society
Pushkarev, D., Neff, N.F., Quake, S.R. 2009 Aug 10.
Single-molecule sequencing of an individual human
genome. Nat Biotechnol
Pharoah, P.D.P, Palmieri, R.T., Ramus, S.J. et al. March
8, 2011. The Role of KRAS rs61764370 in Invasive
Epithelial Ovarian Cancer. Clinical Cancer Research/
American Association of Cancer Research
Lo, Y.M.D. 2009 Jan. Noninvasive prenatal detection of
fetal chromosomal aneuploidies by maternal plasma
nucleic acid analysis: a review of the current state of the
art. BJOG 116:152-157
Domingo, S., Cabeza, C.M., Pruvost, A., Torres,
F., Salazar, J., del Mar Gutierrez, M., Mateo, M.G.,
Fontanet, A., Fernandez, I., Domingo, J.C., Villarroya, F.,
Vidal, F., and Baiget, M. January 31, 2011. Association
of thymidylate synthase gene polymorphisms with
stavudine triphosphate intracellular levels and
lipodystrophy. American Society for Microbiology
Seeb, J.E., Pascal, C.E., Ramakrishnan, R., Seeb, L.W.
2009. SNP genotyping by the 5’-nuclease reaction:
advances in high-throughput genotyping with
nonmodel organisms. A. Komar, editor. Methods in
molecular biology, single nucleotide polymorphisms,
2d edition. Humana Press P. 277-292
Baak-Pablo, R., Dezentje, V., Guchelaar, H., & van
der Straaten, T. September 16, 2010. Genotyping of
DNA Samples Isolated from Formalin-Fixed ParaffinEmbedded Tissues Using Preamplification. Journal of
Molecular Diagnostics
Pander, J., Wessels, J. A. M., Gelderblom, H., van der
Straaten, T., Punt, C.J.A. & Guchelaar, H.-J. August
17, 2010. Pharmacogenetic interaction analysis for the
efficacy of systemic treatment in metastatic colorectal
cancer. Annals of Oncology
SNP GENOTYPING
Wang, J., Lin, M., Crenshaw, A., Hutchinson, A.,
Hicks, B., Yeager, M., Berndt, S., Huang, W.,Hayes,
R.B., Chanock, S.J., Jones, R.C., and Ramakrishnan,
R. 2009 Nov 28. High-throughput single nucleotide
polymorphism genotyping using nanofluidic Dynamic
Arrays. BMC Genomics
HIGH SAMPLE THROUGHPUT
SNP GENOTYPING
© 2011 Fluidigm Corporation. All rights reserved. Fluidigm, the Fluidigm logo, BioMark, Dynamic Array, EP1, FC1, and SNPtype are trademarks or registered trademarks
of Fluidigm Corporation in the U.S. and/or other countries. All other trademarks are the property of their respective owners. Fluidigm recommends that you only purchase
licensed PCR assay reagents from authorized sources.
FOR RESEARCH USE ONLY.
100-4590 1/2012
Corporate Headquarters
7000 Shoreline Court, Suite 100
South San Francisco, CA 94080 USA
Toll-free: 1.866.FLUIDLINE | Fax: 650.871.7152
www.fluidigm.com
Sales
North America | +1 650.266.6170 | [email protected]
Europe/EMEA | +33 1 60 92 42 40 | [email protected]
Japan | +81 3 3555 2351 | [email protected]
Asia | +1 650.266.6170 | [email protected]
INDUSTRY CHALLENGES
The Fluidigm high sample throughput genotyping
solution requires dramatically less manual manipulation
and saves time and money over traditional 384-well
plate applications. The microfluidics architecture does
the work of automatically combining samples and
primer-probe sets into 2,304 to 9,216 PCR reactions
Single nucleotide polymorphism (SNP) genotyping
is a powerful tool in human genetics and agricultural
biotechnology, with applications ranging from the
discovery of human disease modifier regions to sample
identification and tracking to marker-assisted breeding
of livestock and crops. As SNP discovery accelerates
through next-generation sequencing and genome-wide
association studies, there is a critical need for high
throughput, flexible, and cost-effective SNP genotyping
solutions to validate and apply these polymorphisms.
384-well
plate
AGBIO GENOTYPING
Agricultural biologists aim to accentuate valuable traits
in plants and animals for disease resistance, robustness,
and profitability. Wildlife managers work to attain
ecological balance by maintaining the genetic fitness of
wild migratory species. Both fields require low cost, highthroughput SNP genotyping. Our genotyping solution
allows you to quickly and efficiently associate a SNP
combination with a favorable trait for directed breeding
of high yield dairy cows, validate seed populations, or
manage the fitness of a wild salmon population.
OUTSTANDING DATA QUALITY
INCREASED PRODUCTIVITY WITH MICROFLUIDICS
HUMAN GENOTYPING
SNP combinations have been associated with human
traits varying from longevity and obesity to metastatic
and autoimmune disease, resulting in a better
understanding of human metabolism, disease etiology,
and population variation; driving new pharmaceutical
development, and aiding in the advent of personalized
medicine. Our genotyping solution enables you to
quickly and efficiently identify these causal SNP
combinations.
THE FLUIDIGM SOLUTION FOR SNP GENOTYPING
GENOTYPING ASSAYS
46 mL
240 µL
480 µL
576 µL
Primer-probe (20X)
4.6 mL
240 µL
480 µL
72 µL
Plates
24
1
4
2
Time
8 days
4 hours
8 hours
2 hours
Pipette steps
18,432
192
384
432
BIOMARK™ HD SYSTEM
The BioMark HD System sets a new standard for highthroughput real-time gene expression analysis and endpoint genotyping with benefits that are impossible to
reproduce using many other conventional PCR systems.
The IFC technology both prepares and performs
thousands of reactions in nanoliter volumes, saving
both time and money, as well as reducing pipetting
steps by 95%. The system streamlines workflows for
applications demanding sensitivity and dynamic range
at an extremely high throughput.
EP1™ SYSTEM
The EP1 System, with the Dynamic Array IFCs, is
uniquely suited for a range of applications that require
very high sample throughput. These include validation
studies in which many thousands of individuals,
whether from a single seed lot or human population
study, are tested against as many as 1,000 genetic
markers. Validated markers can be integrated into an
assortment of high-throughput applications such as the
following:
breeding and selection
The EP1™ System offers outstanding data quality—even from lower
quality samples—and the most streamlined workflow in the industry.
Genotyping results can be obtained in a matter of hours
with only minutes of hands-on time.
testing
Disease
resistance
Population
Parentage
Core strengths of the Fluidigm systems is their ability
to obtain high call rates and accuracy using standard
chemistries. Here, cattle sample data obtained from the
USDA Agricultural Research Service (ARS) demonstrate
the outstanding results achieved on the EP1 System.
Typical cluster plots are displayed and call rates
achieved on the Dynamic Array IFC are 99.9%.
Allele map with corresponding scatter plots
Comparison of materials and pipetting steps between conventional microplates and Dynamic Array IFCs;
96 samples against 96 assays
Seed
to target species with available sequence
information
96.96
48.48
192.24
Dynamic Array IFC Dynamic Array IFC Dynamic Array IFC
Master mix
Marker-assisted
SNPtype™ Assays provide a high-throughput, lowcost SNP genotyping solution which enables rapid
assay design and polymorphism screening. The
assays are based on allele-specific PCR and combine
the advantages of minimum experimental setup time
and flexible assay choice with the reliability of Dynamic
Array™ Integrated Fluidic Circuit (IFC) technology.
using 200-fold less master mix than traditional systems.
Each microfluidic plate generates 24-fold more data
than that produced by a 384-well plate. This radical
advance in experiment density is fully leveraged
through a hardware/software system that automates
setup and data analysis.
The system integrates thermal cycling and detection of
PCR assays for all Dynamic Array IFCs, and acquires data
for each reaction chamber on the IFC simultaneously
and can operate in either real-time or end-point
detection mode for genotyping experiments.
The BioMark™ HD System significantly improves productivity by
enabling the simultaneous performance of PCR reactions in nanoliter
volumes, collects more data points per day at less cost than 384-well
systems, and enables the use of multiple reagents and
different sample and assay configurations.
genetics
studies
Designed
EASY WORKFLOW WITH THE EP1 SYSTEM
EASY WORKFLOW WITH THE BIOMARK HD SYSTEM
Three-
to four-week design and turnaround time
with customer-provided sequences (minimum of 24
assays per order)
1
Access
to loci-specific primer sequences assures
reproducibility
Compatible
with Specific Target Amplification (STA)
protocol for improving results from samples of
low quality and/or concentration, or from species
with large genome sizes (>human); necessary STA
primers provided
2
3
4
1
2
3
4
Call map view for 48 cattle samples and 48 SNPtype™ Assays (left);
cluster plot for a typical SNPtype Assay (right).
Pipette samples and
SNPtype Assays into the IFC.
Place the IFC onto
the IFC Controller to
automatically set up
genotyping experiments.
Thermal cycle the IFC on the FC1™ Cycler.
Read the IFC on the EP1 Reader in a matter of minutes.
Pipette samples and
SNPtype Assays into the IFC.
Place the IFC onto
the IFC Controller to
automatically set up
genotyping experiments.
Thermal cycle the IFC on the BioMark HD
Reader.
View and analyze results with the data
analysis suite.
INDUSTRY CHALLENGES
The Fluidigm high sample throughput genotyping
solution requires dramatically less manual manipulation
and saves time and money over traditional 384-well
plate applications. The microfluidics architecture does
the work of automatically combining samples and
primer-probe sets into 2,304 to 9,216 PCR reactions
Single nucleotide polymorphism (SNP) genotyping
is a powerful tool in human genetics and agricultural
biotechnology, with applications ranging from the
discovery of human disease modifier regions to sample
identification and tracking to marker-assisted breeding
of livestock and crops. As SNP discovery accelerates
through next-generation sequencing and genome-wide
association studies, there is a critical need for high
throughput, flexible, and cost-effective SNP genotyping
solutions to validate and apply these polymorphisms.
384-well
plate
AGBIO GENOTYPING
Agricultural biologists aim to accentuate valuable traits
in plants and animals for disease resistance, robustness,
and profitability. Wildlife managers work to attain
ecological balance by maintaining the genetic fitness of
wild migratory species. Both fields require low cost, highthroughput SNP genotyping. Our genotyping solution
allows you to quickly and efficiently associate a SNP
combination with a favorable trait for directed breeding
of high yield dairy cows, validate seed populations, or
manage the fitness of a wild salmon population.
OUTSTANDING DATA QUALITY
INCREASED PRODUCTIVITY WITH MICROFLUIDICS
HUMAN GENOTYPING
SNP combinations have been associated with human
traits varying from longevity and obesity to metastatic
and autoimmune disease, resulting in a better
understanding of human metabolism, disease etiology,
and population variation; driving new pharmaceutical
development, and aiding in the advent of personalized
medicine. Our genotyping solution enables you to
quickly and efficiently identify these causal SNP
combinations.
THE FLUIDIGM SOLUTION FOR SNP GENOTYPING
GENOTYPING ASSAYS
46 mL
240 µL
480 µL
576 µL
Primer-probe (20X)
4.6 mL
240 µL
480 µL
72 µL
Plates
24
1
4
2
Time
8 days
4 hours
8 hours
2 hours
Pipette steps
18,432
192
384
432
BIOMARK™ HD SYSTEM
The BioMark HD System sets a new standard for highthroughput real-time gene expression analysis and endpoint genotyping with benefits that are impossible to
reproduce using many other conventional PCR systems.
The IFC technology both prepares and performs
thousands of reactions in nanoliter volumes, saving
both time and money, as well as reducing pipetting
steps by 95%. The system streamlines workflows for
applications demanding sensitivity and dynamic range
at an extremely high throughput.
EP1™ SYSTEM
The EP1 System, with the Dynamic Array IFCs, is
uniquely suited for a range of applications that require
very high sample throughput. These include validation
studies in which many thousands of individuals,
whether from a single seed lot or human population
study, are tested against as many as 1,000 genetic
markers. Validated markers can be integrated into an
assortment of high-throughput applications such as the
following:
breeding and selection
The EP1™ System offers outstanding data quality—even from lower
quality samples—and the most streamlined workflow in the industry.
Genotyping results can be obtained in a matter of hours
with only minutes of hands-on time.
testing
Disease
resistance
Population
Parentage
Core strengths of the Fluidigm systems is their ability
to obtain high call rates and accuracy using standard
chemistries. Here, cattle sample data obtained from the
USDA Agricultural Research Service (ARS) demonstrate
the outstanding results achieved on the EP1 System.
Typical cluster plots are displayed and call rates
achieved on the Dynamic Array IFC are 99.9%.
Allele map with corresponding scatter plots
Comparison of materials and pipetting steps between conventional microplates and Dynamic Array IFCs;
96 samples against 96 assays
Seed
to target species with available sequence
information
96.96
48.48
192.24
Dynamic Array IFC Dynamic Array IFC Dynamic Array IFC
Master mix
Marker-assisted
SNPtype™ Assays provide a high-throughput, lowcost SNP genotyping solution which enables rapid
assay design and polymorphism screening. The
assays are based on allele-specific PCR and combine
the advantages of minimum experimental setup time
and flexible assay choice with the reliability of Dynamic
Array™ Integrated Fluidic Circuit (IFC) technology.
using 200-fold less master mix than traditional systems.
Each microfluidic plate generates 24-fold more data
than that produced by a 384-well plate. This radical
advance in experiment density is fully leveraged
through a hardware/software system that automates
setup and data analysis.
The system integrates thermal cycling and detection of
PCR assays for all Dynamic Array IFCs, and acquires data
for each reaction chamber on the IFC simultaneously
and can operate in either real-time or end-point
detection mode for genotyping experiments.
The BioMark™ HD System significantly improves productivity by
enabling the simultaneous performance of PCR reactions in nanoliter
volumes, collects more data points per day at less cost than 384-well
systems, and enables the use of multiple reagents and
different sample and assay configurations.
genetics
studies
Designed
EASY WORKFLOW WITH THE EP1 SYSTEM
EASY WORKFLOW WITH THE BIOMARK HD SYSTEM
Three-
to four-week design and turnaround time
with customer-provided sequences (minimum of 24
assays per order)
1
Access
to loci-specific primer sequences assures
reproducibility
Compatible
with Specific Target Amplification (STA)
protocol for improving results from samples of
low quality and/or concentration, or from species
with large genome sizes (>human); necessary STA
primers provided
2
3
4
1
2
3
4
Call map view for 48 cattle samples and 48 SNPtype™ Assays (left);
cluster plot for a typical SNPtype Assay (right).
Pipette samples and
SNPtype Assays into the IFC.
Place the IFC onto
the IFC Controller to
automatically set up
genotyping experiments.
Thermal cycle the IFC on the FC1™ Cycler.
Read the IFC on the EP1 Reader in a matter of minutes.
Pipette samples and
SNPtype Assays into the IFC.
Place the IFC onto
the IFC Controller to
automatically set up
genotyping experiments.
Thermal cycle the IFC on the BioMark HD
Reader.
View and analyze results with the data
analysis suite.
INDUSTRY CHALLENGES
The Fluidigm high sample throughput genotyping
solution requires dramatically less manual manipulation
and saves time and money over traditional 384-well
plate applications. The microfluidics architecture does
the work of automatically combining samples and
primer-probe sets into 2,304 to 9,216 PCR reactions
Single nucleotide polymorphism (SNP) genotyping
is a powerful tool in human genetics and agricultural
biotechnology, with applications ranging from the
discovery of human disease modifier regions to sample
identification and tracking to marker-assisted breeding
of livestock and crops. As SNP discovery accelerates
through next-generation sequencing and genome-wide
association studies, there is a critical need for high
throughput, flexible, and cost-effective SNP genotyping
solutions to validate and apply these polymorphisms.
384-well
plate
AGBIO GENOTYPING
Agricultural biologists aim to accentuate valuable traits
in plants and animals for disease resistance, robustness,
and profitability. Wildlife managers work to attain
ecological balance by maintaining the genetic fitness of
wild migratory species. Both fields require low cost, highthroughput SNP genotyping. Our genotyping solution
allows you to quickly and efficiently associate a SNP
combination with a favorable trait for directed breeding
of high yield dairy cows, validate seed populations, or
manage the fitness of a wild salmon population.
OUTSTANDING DATA QUALITY
INCREASED PRODUCTIVITY WITH MICROFLUIDICS
HUMAN GENOTYPING
SNP combinations have been associated with human
traits varying from longevity and obesity to metastatic
and autoimmune disease, resulting in a better
understanding of human metabolism, disease etiology,
and population variation; driving new pharmaceutical
development, and aiding in the advent of personalized
medicine. Our genotyping solution enables you to
quickly and efficiently identify these causal SNP
combinations.
THE FLUIDIGM SOLUTION FOR SNP GENOTYPING
GENOTYPING ASSAYS
46 mL
240 µL
480 µL
576 µL
Primer-probe (20X)
4.6 mL
240 µL
480 µL
72 µL
Plates
24
1
4
2
Time
8 days
4 hours
8 hours
2 hours
Pipette steps
18,432
192
384
432
BIOMARK™ HD SYSTEM
The BioMark HD System sets a new standard for highthroughput real-time gene expression analysis and endpoint genotyping with benefits that are impossible to
reproduce using many other conventional PCR systems.
The IFC technology both prepares and performs
thousands of reactions in nanoliter volumes, saving
both time and money, as well as reducing pipetting
steps by 95%. The system streamlines workflows for
applications demanding sensitivity and dynamic range
at an extremely high throughput.
EP1™ SYSTEM
The EP1 System, with the Dynamic Array IFCs, is
uniquely suited for a range of applications that require
very high sample throughput. These include validation
studies in which many thousands of individuals,
whether from a single seed lot or human population
study, are tested against as many as 1,000 genetic
markers. Validated markers can be integrated into an
assortment of high-throughput applications such as the
following:
breeding and selection
The EP1™ System offers outstanding data quality—even from lower
quality samples—and the most streamlined workflow in the industry.
Genotyping results can be obtained in a matter of hours
with only minutes of hands-on time.
testing
Disease
resistance
Population
Parentage
Core strengths of the Fluidigm systems is their ability
to obtain high call rates and accuracy using standard
chemistries. Here, cattle sample data obtained from the
USDA Agricultural Research Service (ARS) demonstrate
the outstanding results achieved on the EP1 System.
Typical cluster plots are displayed and call rates
achieved on the Dynamic Array IFC are 99.9%.
Allele map with corresponding scatter plots
Comparison of materials and pipetting steps between conventional microplates and Dynamic Array IFCs;
96 samples against 96 assays
Seed
to target species with available sequence
information
96.96
48.48
192.24
Dynamic Array IFC Dynamic Array IFC Dynamic Array IFC
Master mix
Marker-assisted
SNPtype™ Assays provide a high-throughput, lowcost SNP genotyping solution which enables rapid
assay design and polymorphism screening. The
assays are based on allele-specific PCR and combine
the advantages of minimum experimental setup time
and flexible assay choice with the reliability of Dynamic
Array™ Integrated Fluidic Circuit (IFC) technology.
using 200-fold less master mix than traditional systems.
Each microfluidic plate generates 24-fold more data
than that produced by a 384-well plate. This radical
advance in experiment density is fully leveraged
through a hardware/software system that automates
setup and data analysis.
The system integrates thermal cycling and detection of
PCR assays for all Dynamic Array IFCs, and acquires data
for each reaction chamber on the IFC simultaneously
and can operate in either real-time or end-point
detection mode for genotyping experiments.
The BioMark™ HD System significantly improves productivity by
enabling the simultaneous performance of PCR reactions in nanoliter
volumes, collects more data points per day at less cost than 384-well
systems, and enables the use of multiple reagents and
different sample and assay configurations.
genetics
studies
Designed
EASY WORKFLOW WITH THE EP1 SYSTEM
EASY WORKFLOW WITH THE BIOMARK HD SYSTEM
Three-
to four-week design and turnaround time
with customer-provided sequences (minimum of 24
assays per order)
1
Access
to loci-specific primer sequences assures
reproducibility
Compatible
with Specific Target Amplification (STA)
protocol for improving results from samples of
low quality and/or concentration, or from species
with large genome sizes (>human); necessary STA
primers provided
2
3
4
1
2
3
4
Call map view for 48 cattle samples and 48 SNPtype™ Assays (left);
cluster plot for a typical SNPtype Assay (right).
Pipette samples and
SNPtype Assays into the IFC.
Place the IFC onto
the IFC Controller to
automatically set up
genotyping experiments.
Thermal cycle the IFC on the FC1™ Cycler.
Read the IFC on the EP1 Reader in a matter of minutes.
Pipette samples and
SNPtype Assays into the IFC.
Place the IFC onto
the IFC Controller to
automatically set up
genotyping experiments.
Thermal cycle the IFC on the BioMark HD
Reader.
View and analyze results with the data
analysis suite.
PUBLICATIONS
Spitz, M.R., Gorlov, I.P., Amos, C.I., Dong, Q., Chen, W.,
Etzel, C.J., Gorlova, O.Y., Chang, D.W., Pu, X., Zhang,
D., Wang, L., Cunningham, J.M., Yang, P., and Wu, X.
August 25, 2011. Variants in Inflammation Genes Are
Implicated in Risk of Lung Cancer in Never Smokers
Exposed to Second-hand Smoke. Cancer Discovery
Paez, D., Pare, L., Espinosa, I., Salazar, J., Del Rio, E.,
Barnadas, A., Marcuello, E. and Baiget, M. May 18, 2010.
Immunoglobulin G fragment C receptor polymorphisms
and KRAS mutations: Are they useful biomarkers of
clinical outcome in advanced colorectal cancer treated
with anti-EGFR-based therapy? Cancer Science
Chu, X. Song, H.D., et al. August 14, 2011. A genomewide association study identifies two new risk loci for
Graves’ disease. Nature Genetics
Sagreiya, H., Berube, C., Wen, A., Ramakrishnan, R., Mir,
A., Hamilton, A., Altman, R.B. May 3, 2010. Extending
and evaluating a warfarin dosing algorithm that
includes CYP4F2 and pooled rare variants of CYP2C9.
Pharmacogenetics and Genomics
K. Shitara, S. Ito, K. Misawa, Y. Ito, H. Ito, S. Hosono,
M. Watanabe, K. Tajima, H. Tanaka, K. Muro, and K.
Matsuo. June 20, 2011. Genetic polymorphism of IGF-I
predicts recurrence in patients with gastric cancer
who have undergone curative gastrectomy. Annals of
Oncology
Chan, M., Chan, M.W., Loh, T.W., Law, H.Y., Yoon, C.S.,
Than, S.S., Chua, J.M., Wong, C.Y.,Yong, W.S., Yap, Y.S.,
Ho, G.H., Ang, P, and Lee, A.S.G. May 2011. Evaluation
of Nanofluidics Technology for High-Throughput SNP
Genotyping in a Clinical Setting. Journal of Molecular
Diagnostics
P. J. Maughan, S. M. Smith, D. J. Fairbanks, and E. N.
Jellen. March 16, 2011. Development, characterization
and linkage mapping of single nucleotide polymorphisms
in the gram Amaranths. The Plant Genome
Habicht, C., Seeb, L. W., Myers, K. W., Farley, E.V.,
Seeb, J. E. 2010. Summer-Fall Distribution of Stocks of
Immature Sockeye Salmon in the Bering Sea as Revealed
by Single-Nucleotide Polymorphisms. Transactions of
the American Fisheries Society
Pushkarev, D., Neff, N.F., Quake, S.R. 2009 Aug 10.
Single-molecule sequencing of an individual human
genome. Nat Biotechnol
Pharoah, P.D.P, Palmieri, R.T., Ramus, S.J. et al. March
8, 2011. The Role of KRAS rs61764370 in Invasive
Epithelial Ovarian Cancer. Clinical Cancer Research/
American Association of Cancer Research
Lo, Y.M.D. 2009 Jan. Noninvasive prenatal detection of
fetal chromosomal aneuploidies by maternal plasma
nucleic acid analysis: a review of the current state of the
art. BJOG 116:152-157
Domingo, S., Cabeza, C.M., Pruvost, A., Torres,
F., Salazar, J., del Mar Gutierrez, M., Mateo, M.G.,
Fontanet, A., Fernandez, I., Domingo, J.C., Villarroya, F.,
Vidal, F., and Baiget, M. January 31, 2011. Association
of thymidylate synthase gene polymorphisms with
stavudine triphosphate intracellular levels and
lipodystrophy. American Society for Microbiology
Seeb, J.E., Pascal, C.E., Ramakrishnan, R., Seeb, L.W.
2009. SNP genotyping by the 5’-nuclease reaction:
advances in high-throughput genotyping with
nonmodel organisms. A. Komar, editor. Methods in
molecular biology, single nucleotide polymorphisms,
2d edition. Humana Press P. 277-292
Baak-Pablo, R., Dezentje, V., Guchelaar, H., & van
der Straaten, T. September 16, 2010. Genotyping of
DNA Samples Isolated from Formalin-Fixed ParaffinEmbedded Tissues Using Preamplification. Journal of
Molecular Diagnostics
Pander, J., Wessels, J. A. M., Gelderblom, H., van der
Straaten, T., Punt, C.J.A. & Guchelaar, H.-J. August
17, 2010. Pharmacogenetic interaction analysis for the
efficacy of systemic treatment in metastatic colorectal
cancer. Annals of Oncology
SNP GENOTYPING
Wang, J., Lin, M., Crenshaw, A., Hutchinson, A.,
Hicks, B., Yeager, M., Berndt, S., Huang, W.,Hayes,
R.B., Chanock, S.J., Jones, R.C., and Ramakrishnan,
R. 2009 Nov 28. High-throughput single nucleotide
polymorphism genotyping using nanofluidic Dynamic
Arrays. BMC Genomics
HIGH SAMPLE THROUGHPUT
SNP GENOTYPING
© 2011 Fluidigm Corporation. All rights reserved. Fluidigm, the Fluidigm logo, BioMark, Dynamic Array, EP1, FC1, and SNPtype are trademarks or registered trademarks
of Fluidigm Corporation in the U.S. and/or other countries. All other trademarks are the property of their respective owners. Fluidigm recommends that you only purchase
licensed PCR assay reagents from authorized sources.
FOR RESEARCH USE ONLY.
100-4590 1/2012
Corporate Headquarters
7000 Shoreline Court, Suite 100
South San Francisco, CA 94080 USA
Toll-free: 1.866.FLUIDLINE | Fax: 650.871.7152
www.fluidigm.com
Sales
North America | +1 650.266.6170 | [email protected]
Europe/EMEA | +33 1 60 92 42 40 | [email protected]
Japan | +81 3 3555 2351 | [email protected]
Asia | +1 650.266.6170 | [email protected]
PUBLICATIONS
Spitz, M.R., Gorlov, I.P., Amos, C.I., Dong, Q., Chen, W.,
Etzel, C.J., Gorlova, O.Y., Chang, D.W., Pu, X., Zhang,
D., Wang, L., Cunningham, J.M., Yang, P., and Wu, X.
August 25, 2011. Variants in Inflammation Genes Are
Implicated in Risk of Lung Cancer in Never Smokers
Exposed to Second-hand Smoke. Cancer Discovery
Paez, D., Pare, L., Espinosa, I., Salazar, J., Del Rio, E.,
Barnadas, A., Marcuello, E. and Baiget, M. May 18, 2010.
Immunoglobulin G fragment C receptor polymorphisms
and KRAS mutations: Are they useful biomarkers of
clinical outcome in advanced colorectal cancer treated
with anti-EGFR-based therapy? Cancer Science
Chu, X. Song, H.D., et al. August 14, 2011. A genomewide association study identifies two new risk loci for
Graves’ disease. Nature Genetics
Sagreiya, H., Berube, C., Wen, A., Ramakrishnan, R., Mir,
A., Hamilton, A., Altman, R.B. May 3, 2010. Extending
and evaluating a warfarin dosing algorithm that
includes CYP4F2 and pooled rare variants of CYP2C9.
Pharmacogenetics and Genomics
K. Shitara, S. Ito, K. Misawa, Y. Ito, H. Ito, S. Hosono,
M. Watanabe, K. Tajima, H. Tanaka, K. Muro, and K.
Matsuo. June 20, 2011. Genetic polymorphism of IGF-I
predicts recurrence in patients with gastric cancer
who have undergone curative gastrectomy. Annals of
Oncology
Chan, M., Chan, M.W., Loh, T.W., Law, H.Y., Yoon, C.S.,
Than, S.S., Chua, J.M., Wong, C.Y.,Yong, W.S., Yap, Y.S.,
Ho, G.H., Ang, P, and Lee, A.S.G. May 2011. Evaluation
of Nanofluidics Technology for High-Throughput SNP
Genotyping in a Clinical Setting. Journal of Molecular
Diagnostics
P. J. Maughan, S. M. Smith, D. J. Fairbanks, and E. N.
Jellen. March 16, 2011. Development, characterization
and linkage mapping of single nucleotide polymorphisms
in the gram Amaranths. The Plant Genome
Habicht, C., Seeb, L. W., Myers, K. W., Farley, E.V.,
Seeb, J. E. 2010. Summer-Fall Distribution of Stocks of
Immature Sockeye Salmon in the Bering Sea as Revealed
by Single-Nucleotide Polymorphisms. Transactions of
the American Fisheries Society
Pushkarev, D., Neff, N.F., Quake, S.R. 2009 Aug 10.
Single-molecule sequencing of an individual human
genome. Nat Biotechnol
Pharoah, P.D.P, Palmieri, R.T., Ramus, S.J. et al. March
8, 2011. The Role of KRAS rs61764370 in Invasive
Epithelial Ovarian Cancer. Clinical Cancer Research/
American Association of Cancer Research
Lo, Y.M.D. 2009 Jan. Noninvasive prenatal detection of
fetal chromosomal aneuploidies by maternal plasma
nucleic acid analysis: a review of the current state of the
art. BJOG 116:152-157
Domingo, S., Cabeza, C.M., Pruvost, A., Torres,
F., Salazar, J., del Mar Gutierrez, M., Mateo, M.G.,
Fontanet, A., Fernandez, I., Domingo, J.C., Villarroya, F.,
Vidal, F., and Baiget, M. January 31, 2011. Association
of thymidylate synthase gene polymorphisms with
stavudine triphosphate intracellular levels and
lipodystrophy. American Society for Microbiology
Seeb, J.E., Pascal, C.E., Ramakrishnan, R., Seeb, L.W.
2009. SNP genotyping by the 5’-nuclease reaction:
advances in high-throughput genotyping with
nonmodel organisms. A. Komar, editor. Methods in
molecular biology, single nucleotide polymorphisms,
2d edition. Humana Press P. 277-292
Baak-Pablo, R., Dezentje, V., Guchelaar, H., & van
der Straaten, T. September 16, 2010. Genotyping of
DNA Samples Isolated from Formalin-Fixed ParaffinEmbedded Tissues Using Preamplification. Journal of
Molecular Diagnostics
Pander, J., Wessels, J. A. M., Gelderblom, H., van der
Straaten, T., Punt, C.J.A. & Guchelaar, H.-J. August
17, 2010. Pharmacogenetic interaction analysis for the
efficacy of systemic treatment in metastatic colorectal
cancer. Annals of Oncology
SNP GENOTYPING
Wang, J., Lin, M., Crenshaw, A., Hutchinson, A.,
Hicks, B., Yeager, M., Berndt, S., Huang, W.,Hayes,
R.B., Chanock, S.J., Jones, R.C., and Ramakrishnan,
R. 2009 Nov 28. High-throughput single nucleotide
polymorphism genotyping using nanofluidic Dynamic
Arrays. BMC Genomics
HIGH SAMPLE THROUGHPUT
SNP GENOTYPING
© 2011 Fluidigm Corporation. All rights reserved. Fluidigm, the Fluidigm logo, BioMark, Dynamic Array, EP1, FC1, and SNPtype are trademarks or registered trademarks
of Fluidigm Corporation in the U.S. and/or other countries. All other trademarks are the property of their respective owners. Fluidigm recommends that you only purchase
licensed PCR assay reagents from authorized sources.
FOR RESEARCH USE ONLY.
100-4590 1/2012
Corporate Headquarters
7000 Shoreline Court, Suite 100
South San Francisco, CA 94080 USA
Toll-free: 1.866.FLUIDLINE | Fax: 650.871.7152
www.fluidigm.com
Sales
North America | +1 650.266.6170 | [email protected]
Europe/EMEA | +33 1 60 92 42 40 | [email protected]
Japan | +81 3 3555 2351 | [email protected]
Asia | +1 650.266.6170 | [email protected]