NEXTGEN SEQUENCING NxSeqTM DNA Sample Prep Kit Te future of DNA library prep for Next Generation Sequencing  Save up to 2 hours o your library prep  Reduce chimeras and false junctions All without compromising  Cut NGS costs Next Generation Sequencing (NGS) requires a high performance library preparation kit that drives effective end repair, A-tailing, and adapter ligation . NxSeq TM DNA Sample Prep Kits are the only ones optimized for highly efficient A-tailing and ligation, ensuring peak performance on your sequencing platform. In addition, the NxSeq kits have one of the most efficient workflows available. Get sequence ready libraries in less than 2 hours with just a few simple steps. data quality. Time Comparison Fr om Shear ed DNA t o Si ze S e l e c t i o n Walk-Away Time vs. Hands-On Lab Time 70 40 100 75 70 165 Available for: Roche 454 & Illumina Platforms. Ion Torrent kits coming soon. 1500 110 min 175 min 235 min Lucigen NEB Illumina NxSeq 0 Less hands-on time, twice as fast as Illumina 1000000200000030000004000000 1500 NEB 0 1000000200000030000004000000 1500 Illumina 0 1000000200000030000004000000 Fig u r e 1 . C o v e ra ge c o mp a r ison o f D H 1 0B G e n o mic D N A lib r a r ies . L u c ig e n ' s N x Se q D N A Sa mp le Pr e p Kit 2 , N EB' s N EBN ex t D N A Sa mp le Pr e p Ma s te r Mix Se t 1 , a n d Illu min a ' s Tr u Se q TM Sa mp le Pr e p k it - v 2 w e r e u s e d to c r e a te N G S lib r a r ie s fr o m D H 1 0 B E. c o li g e n o mic D N A. All s a mp le s w e r e b a r c o d e d a n d r u n o n a n Illu min a G AIIx . Plo ts in d ic a te d e p th o f c o v e r a g e a c h ie v e d in a mo v in g 1 0 0 b a s e r e a d w in d o w . Eq u iv a le n t p e r fo r ma n c e w a s o b ta in e d fo r a ll k its . LuBioScience GmbH, Töpferstrasse 5, 6004 Luzern T.: 041 417 02 80, F.: 041 417 02 89 / E.: [email protected], W.: www.lubio.ch NxSeq DNA Sample Prep Kit Workflow TM Twice as fast as Illumina From Sheared DNA to Size Selection Lucigen Lucigen 1 hr 50 min Hands-On Lab Time Walk-Away Time Size Selection 5 40 5 30 30 Minutes Incubation End Repair/ A-tail Setup Incubation Clean Up Ligation Setup NEB Sheared DNA NEB 2 hrs 55 min 5 30 30 5 30 30 5 15 30 Minutes Incubation Clean Up End Repair Setup Incubation Incub. Clean Up A-Tail Setup Clean Up Ligation Setup Illumina 1 lumina 1 3 hrs 55 min 5 30 60 5 30 5 10 90 Minutes Incubation End Repair Setup 0 Products NxSeqTM DNA Sample Prep Kit 1 (Roche 454 Compatible) NxSeqTM DNA Sample Prep Kit 2 (Illumina Compatible) Incubation Clean Up A-tail Setup 1 Hr Incub. Clean Up Ligation Setup 2 Hrs 3 Hrs Size Cat. No. 10 rxns 11000-1 20 rxns 11000-2 10 rxns 12000-1 20 rxns 12000-2 ORDER INFORMATION The NxSeq DNA Sample Prep Kits 1 and 2 both contain NxSeq Enzyme Mix , NxSeq 2X Buffer , and NxGenTM High Concentration Ligase . NxSeq D N A Sa m p l e P r e p K i t 1 a l s o c o n t a i n s D N A S u s p e n s i o n Bu f f e r a n d S i z i n g Solution. Roche 454 platform users should order Kit 1, while Illumina u s e r s s h o u l d o r d e r K i t 2 . P l a t f o r m - s p e c i f ic a d a p t e r s a r e n o t i n c l u d e d a n d must be supplied by the user. Complete protocols are included. LuBioScience GmbH, Töpferstrasse 5, 6004 Luzern T.: 041 417 02 80, F.: 041 417 02 89 / E.: [email protected], W.: www.lubio.ch 4 Hrs