Agilent AssayMAP Cartridges Automated Sample Preparation Data Sheet Key Features Introduction • Perform quantitation and sample preparation in the same workflow Agilent AssayMAP is a quantitative, high-throughput sample preparation technology which uses disposable chromatography cartridges on the Bravo Platform for AssayMAP. AssayMAP technology relies on a modified 96-channel, microplatecompatible liquid handler equipped with precision flow syringes that connect to the chromatography cartridges through a special probe. The AssayMAP Bravo head provides precise, positive-displacement flow control through the cartridges in either direction to well under 1 µL/min. The cartridges incorporate a 5 µL packed bed that can accommodate virtually any polymer, silica, or gel-based resin with particle diameters from 15 to 100 µm. Included are the specifications for four prepacked AssayMAP cartridges. • Reduce errors and increase walkaway time with reliable, automated processess • Increase throughput using existing chemistry or customized workflows AssayMAP Cartridge Release Criteria Each batch of resin slurry that serves as the packing material for multiple cartridge racks is packed into cartridges and representative cartridges per batch are functionally tested for dynamic binding. Every AssayMAP cartridge is pressure/flow tested and visually inspected. The flow test measures the pressure drop across the cartridge at a specified flow rate. Visual inspection verifies correct assembly and complete filling of the bed volume. AssayMAP Cartridge Reuse Performance is guaranteed when cartridges are used on the same day they are equilibrated. Do not allow hydrated cartridges to dry out. Keep hydrated cartridges in a cartridge rack for same-day storage off the deck by stacking the rack on a receiver plate containing 200 µL equilibration buffer per well, covering with a rack lid, and placing the assembly in a sealed bag. We do not guarantee performance of stored cartridges following equilibration. Ordering Information Cartridge Part number Cartridge code Contents Resin Particle size range Bed volume Chemistry Bead Ligand Capacity Dynamic binding capacity Protein A G5496-60000 PA-W 1 rack/96 cartridges Protein G G5496-60008 PG-W 1 rack/96 cartridges 53 to 61 µm 5 µL 45 to 53 µm 5 µL Synthetic polymer Recombinant Protein A, produced in E. coli Synthetic polymer Recombinant Protein G, produced in E. coli. 10 % breakthrough point: ≥100 µg human IgG (hIgG) per cartridge 10 % breakthrough point: ≥100 µg human IgG (hIgG) per cartridge Saturation binding capacity ≥125 µg hIgG per cartridge ≥150 µg hIgG per cartridge 2–11 (short exposure to 0.1 N NaOH is acceptable for cleaning) 2–11 (short exposure to 0.1 N NaOH is acceptable for cleaning) Stability pH Range 6–8 routine use Temperature Storage 4 °C to room temperature Room temperature (store as packaged from manufacturer) 4 °C to room temperature Room temperature (store as packaged from manufacturer) Expiration Use within 6 months of purchase Use within 6 months of purchase Purification and quantitation of monoand polyclonal antibodies and Fc fusion proteins with affinity for Protein A. Purification and quantitation of mono- and polyclonal antibodies and Fc fusion proteins with affinity for Protein G. Applications 2 Cartridge Part number Cartridge code Contents Resin Pore size Particle size range Bed volume Chemistry Bead Ligand Capacity Dynamic binding capacity Saturation binding capacity Stability pH Range Streptavidin G5496-60010 SA-W 1 rack/96 cartridges Reversed Phase G5496-60033 RP-S 1 rack/96 cartridges 45–53 µm 5 µL 100 Å 15–20 µm 5 µL Synthetic polymer Streptavidin, recombinant Underivatized polystyrene-divinylbenzene none Biotinylated IgG: ≥ 90 µg (≥ 600 pmol) Biotinylated BSA: ≥ 50 µg (≥ 750 pmol) Biotinylated FITC: ND Biotinylated IgG: ~100 µg (~700 pmol) Biotinylated BSA: ~60 µg (~900 pmol) Biotinylated FITC: ~3.5 µg (~5000 pmol) Quantitative binding of ≥ 450 µg human insulin at 2 µL/min flow rate 500 µg human insulin 2–10 short-term use 6–8 routine use 1–14 Temperature Storage 4 °C to room temperature 4–10 °C (store as packaged from manufacturer) Up to 200 °C Room temperature (store as packaged from manufacturer) Expiration Use within 6 months of purchase Use within 6 months of purchase Generation of affinity cartridges by immobilizing biotinylated molecules. Cartridges are suitable for subsequent use purifying and quantitating ligand binding reactions. Concentrating, desalting and fractionating mixtures of peptides and small proteins prior to mass spectroscopy. Applications 3 Learn More www.agilent.com/lifesciences/AssayMAP Find a local Agilent customer center www.agilent.com/chem/contactus USA and Canada 1-800-227-9770 [email protected] Europe [email protected] Asia Pacific [email protected] This information is subject to change www.agilent.com/chem/xxxxx without notice. For Research Use Only. Not for use in diagnostic procedures. © Agilent Technologies, Inc., 2011 Published in Country, Month xx, 2011 © Agilent Technologies, Inc., 2013 Publication Number xxxx-xxxxEN Published in the USA, January 14, 2013 5991-1774EN