Chromatography Columns and Supplies Catalog www.waters.com
Chromatography Columns and Supplies Catalog 2001–2002 www.waters.com What’s New See Thousands of Products. See Applications. See Pricing and Availability. See Dot Shop. c.shop™ – The “Catalog with a Brain” and your online resource for searching and buying the world’s best chromatography columns sample preparation products, accessories and Waters Quality parts. Easily search and view product, price and availability for thousands of products. No need to know the part number and product name in order to find the product. Our easy to use product finder guides you through the specifications that best meets your needs. Links to our application database and other technical content makes this a powerful resource for scientists and purchasing agents. Because the site is tied into Waters enterprise information network, you can check product availability, pricing and delivery status. Initial registration is required for first-time buyers. Then just simply log in the next time and begin shopping. 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Now you can consolidate more of your purchasing while still drawing on a broad selection of alternative HPLC columns. In addition, every column we sell is backed by your local Waters technical and customer support. Because Waters brings together the product selection, manufacturing expertise, research and development, worldwide distribution, and the applications support you need from your primary supplier of HPLC chromatography consumable products, you won’t find a broader, more productive resource than Waters Corporation. Visit us at www.waters.com Waters Chromatography Chemical Manufacturing: "In a Class by Itself" by Frederick G. Harrison, Director, Manufacturing Operations Waters manufactures its consumable chromatography products in Taunton, Massachusetts and in a recently opened facility in Wexford, Ireland. Wexford is located 90 miles south of Dublin on Ireland's southeast coast. Our manufacturing operations continue to be unique as compared to others in the industry. Our primary mission is to synthesize high-quality chromatography supports, pack chromatography columns, assemble solid-phase extraction devices, and to produce reagent kits. Since some of our products are utilized as compounds in medical devices, our facilities are registered with the U.S. Food and Drug Administration as Medical Device manufacturers, operating under current Good Manufacturing Practices (cGMP) as outlined in the Federal Register, Title 21, Part 820. Both plants are also ISO-9002 certified by Lloyds Register, Quality Assurance, Registration number 100,238. The 33,000 sq. ft. Taunton, Massachusetts facility, built in 1978, is actually two buildings in one: an explosion proof chemical processing area, and the rest of the facility which includes specialty column packing and solid-phase extraction assembly, quality control, a stockroom, and administration offices. In addition, on the ten-acre site is a state-of-the-art solvent storage and handling facility, and a bunkered warehouse for the storage of drummed chemicals. Our brand new two-story 25,000-sq. ft. Wexford, Ireland facility sits on 4 acres of land. This facility manufactures the bulk of our chromatographic columns and solid-phase extraction devices. Similar to Taunton, it also contains quality control, finished goods stores, administration offices, and a bunkered warehouse. Chemical Processing The chemical processing section of the Taunton facility manufacturers over 200 different silica or polymer-based bulk chromatographic materials and reagents including XTerra™, Symmetry®, Nova-Pak®, µBondapak™, and Waters Spherisorb® products. Chromatographic particles are first made and then sized using air or mechanical classification. We then chemically bond various ligands to the base particle to provide you with a range of selectivity, such as C18, C8, phenyl, and so forth. These reactions take place in glass-lined Pfaudler reactors, ranging in size from 5 gallons to 100 gallons. These bonded particles are then isolated from the reaction mixtures and dried. Throughout this entire process, highly skilled chemical technicians follow detailed operating procedures very much like those used in the pharmaceutical industry. After each step, technicians indicate completion of a given operation by recording the time, date, and their initials on batch records. These processes are also monitored in our quality laboratory. Samples are taken at critical points in the operation and results are then analyzed and compared to in-process specifications. Finally, the finished bulk packing is tested in the quality control laboratory both chemically and chromatographically to ensure that it meets the same level of performance as previously manufactured material--giving you the same reproducibility each time you use a Waters’ chromatographic product. Column Packing and Solid-Phase Extraction Assembly at Both Facilities To provide you with the highest quality chromatographic product, skilled technicians carefully pack over two thousand different steel columns, polyethylene cartridges, solid-phase extraction cartridges and reagent kits according to detailed operating procedures, like the ones described earlier. Once the columns are packed, they are chromatographically tested to ensure quality and consistency using state-of-the-art equipment. Solid-phase extraction devices are tested for backpressure, flow rate, and in some cases, extraction efficiencies. Each column or lot of extraction devices is then labeled with a serial number and packaged. This serial number provides traceability for every column and device lot and documents complete batch information for the packing material used in the finished product. We can tell you when it was tested, who tested it, and the results of the test for a chromatographic product manufactured 3 months or 3 years ago. This documentation guarantees that when you purchase your next Waters column or device, it will perform the same way as your last column or device. We are proud of our manufacturing facilities and invite you to visit us. Tours of either site can be arranged by your Waters Technical Representative. Limited Product Warranty Waters Corporation provides this limited warranty (the Warranty) to protect customers from noncomformity in the product workmanship or materials. The Warranty covers all new product manufactured by Waters and its subsidiaries. The Warranty is as follows: Waters warrants that all products sold by them will be of good quality and workmanship. The products will be fit for their intended purposes when used strictly in accordance with Waters instructions for use during the applicable warranty period. The foregoing warranty is exclusive and in lieu of all other express and implied warranties including, but not limited, to fitness for any other purposes(s). In no event will Waters be liable for consequential, economic or incidental damages of any nature. Waters reserves the right not to honor this warranty if the products are abused by the customer. The warranty will not be deemed to have failed of its essential purpose so long as Waters is able and willing to repair or replace any non-conforming part or product. All prices are subject to change without notification. Trademark Acknowledgements Trademarks of Waters Corporation Accell, AccQ-Fluor, AccQ•Tag, AccuPure, AccuSep, Alliance, Bondapak, µBondapak, CapLC, C-Gel, Delta-Pak, EnantioSelect, Envirogel, Gen-Pak FAX, Guard-Pak, IC-Pak, IonSelect, Nova-Pak, Oasis, Pico•Tag, Porapak, Porasil, µPorasil, PrepLC, PrepPak, Protein-Pak, Radial-Pak, Resolve, SAM, Sentry, Sep-Pak, Silica-Pak, Styragel, µStyragel, Sugar-Pak, Symmetry, SymmetryPrep, SymmetryShield, Symmetry300, Ultrahydrogel, Ultrastyragel, XPoSure, Waters Spherisorb, XTerra, c.shop and Waters All other trademarks in the catalog are acknowledged. Acrodisc AcroPrep Cheminert Florisil FPLC Inter-Seal µPage Partisil Shodex Slip-Free Supor Z-Spin Pall Gelman Laboratory Pall Gelman Laboratory VICA US Silica Corporation. Pharmacia LKB Integrated Liner Technologies, Inc. J&W Whatman Showa Denko Keystone Scientific, Inc. Pall Gelman Laboratory Pall Gelman Laboratory Click anywhere on Table of Contents; Type desired page number; Click on OK. Sample Preparation Page Solid-Phase Extraction Sample Preparation Introduction......................................................................................6 Speciality Products .........................................................................7 Oasis® Sample Extraction Products......................................................7 Porapak® RDX Sep-Pak® Extraction Cartridge.......................................24 Sep-Pak® DNPH-Silica Cartridges .....................................................22 Waters XPoSure™ Aldehyde Sampler Cartridge ..................................23 Ordering Information Oasis® Sample Extraction Products ...........................................20 Speciality Cartridges ..............................................................25 Waters Solid-Phase Extraction Applications Guide and Bibliography, 6th Edition ..................................................26 Sep-Pak Cartridges and Accessories .............................................26 General Elution Protocols on Sep-Pak® Cartridges ...............................33 Sep-Pak® Cartridge Selection Guide .................................................34 Which Cartridge is Best for Your Application .....................................27 Which Sorbent is Best for Your Application ........................................29 Ordering Information Sep-Pak® Cartridges...............................................................36 Waters Extraction Manifold and Accessories .............................40 Sample and Solvent Filtration Alliance® Filtration System ................................................................42 Ordering Information AcroPrep™ Filter Plates for the Alliance® Filtration System...............43 AcroPrep™ Vials, Accessories and Spare Parts............................43 Pall Gelman Laboratory Sample and Solvent Filtration Products Introduction ...........................................................................44 Filter Design and Membrane Choices.......................................45 Part Number Cross Reference..................................................46 Ordering Information Solvent Filtration Membranes and Apparatus .............................50 Syringe Filters ........................................................................47 5 Sample Preparation Introduction Sample preparation is a key procedure in modern chemical analysis. By some estimates, 60-80% of the work activity and operating cost in an analytical laboratory is spent preparing samples for introduction into an analytical system or device. The importance of sample preparation stems from three major concerns: Sample Concentration Frequently, the component of interest is present in levels too low for detection. Sample preparation can concentrate the component to necessary levels for accurate and precise measurement. Contaminants The presence of interfering matrix elements can mask or interfere with the analysis of the component(s) of interest. Sample preparation can remove excess contaminants to yield clean, informative chromatograms. Strategies for Solid-Phase Extraction There are two simple solid-phase extraction strategies for sample preparation. You can choose a cartridge sorbent and sample solvent to cause the component(s) of interest to be: 1. unretained while matrix interferences are adsorbed 2 retained while matrix interferences pass through unretained The first strategy is usually chosen when the desired sample component is present in high concentration. When components of interest are present at low levels, or multiple components of widely differing polarities need to be isolated, the second strategy is generally employed. The second strategy may also be used for trace enrichment of extremely low level compounds and concentration of dilute samples. A complex matrix may be treated by both elution strategies to isolate different target analytes. With either strategy, there are three different chromatographic modes to choose from. You can select : In Solution For most analyses (HPLC, GC, Spectrophometry, RIA, etc), the sample must be properly prepared in solution for subsequent analysis. • normal phase Common methods for sample preparation include liquid-liquid extraction, centrifugation, and solid-phase extraction. Solid phase extraction (SPE) is one of the simplest, yet most effective and versatile, methods of sample preparation. • ion-exchange Utilizing low cost, prepacked, disposable cartridges, a sample component of interest is separated from other species by applying the sample mixture to a solid chromatographic sorbent and selectively eluting the desired component(s). Convenient, Easy-to-Use Convenience and ease-of-use are primary benefits of Sep-Pak® solidphase extraction cartridges, to maximize the productivity of your analytical operation while saving you time and money. When compared to other sample preparation techniques, SPE cartridges also offer: Faster sample prep — average time cut by 2/3 Lower cost — less solvent and reagent consumption and less hazardous waste for disposal • reversed-phase The basic characteristics of each chromatographic mode are described on page 25. There are many different types of sorbents for each mode and the selection of strategy, mode, sorbent, and elution solvents will depend upon the specific sample mixture and goal of the separation. For example, the first strategy may be used prior to the analysis of organic acids in red wine. By passing the wine sample at the appropriate pH through a C18 reversed-phase cartridge, the interfering wine pigments are removed by adsorption while the organic acids elute unretained. With a similar sample but a different goal, the second strategy can be used to isolate phenolic pigments in red wine, also with a C18 cartridge. In this case, the sugars and acids pass directly through the cartridge, while the pigments are retained and subsequently eluted with a stronger mobile phase. Of course, it is possible to combine two such applications into a single, multi-step protocol to prepare several classes of compounds for analysis. Greater recoveries — minimal sample transfer Greater accuracy — no cross contamination Less sample handling — no emulsion problems Reduced harm to labile samples — minimal evaporation Improved safety — due to reduced solvent/sample exposure and glassware Easy automation — simultaneous batch processing of multi-samples © 2001 Waters Corporation 6 Sample Preparation Oasis® Family of HLB, MCX & MAX Sample Extraction Products Sample Preparation Setting New Standards for Solid-Phase Extraction (SPE) Technology The extensive offering of the Oasis® sample extraction products allows you to select the device with the sorbent amount that meets your assay requirements. Available chemistries: HLB (Hydrophilic-Lipophilic Balance) reversed-phase sorbent. • Three Polymeric Sorbents, pH Stable 0-14 • High and Consistent Recoveries • Unmatched Batch-to-Batch Reproducibility • Simple and Fast SPE Method Development • 1 – 35 cc Sample Extraction Cartridges with HLB, MAX, MCX Sorbents • 96-Well Extraction Plates with HLB, MCX and MAX Sorbents for High Throughput LC/MS/MS • Sample Extraction Columns and Prospekt™ Cartridges with HLB Sorbent • HLB Glass Sample Extraction Cartridge • Bulk and Other Products available Upon Request MCX (Mixed-mode: Cation-eXchange and reversed-phase) sorbent. MAX (Mixed-mode: Anion-eXchange and reversed-phase) sorbent. Current Oasis Patents: ® Patent No. 5,882,521 (1996), Patent No. 5,976,376 (1998) Patent No. 6,106,721 (1999), Additional Patents Pending © 2001 Waters Corporation 7 Sample Preparation Oasis® HLB plates, cartridges and columns High and Consistent Recoveries Oasis® sorbents are water-wettable maintaining high retention and capacity for a wide spectrum of analytes, especially when the SPE column runs dry. Oasis® sorbents retain polar compounds far better than bonded silica SPE sorbents, figure 2. High Capacity When transferring methods from a C18-bonded phase to Oasis® products, keep in mind the greater capacity of the Oasis® sorbent. The Oasis® sorbent has 2-3x more surface area and shows a dramatic increase in k values compared to silica-based C18, figure 3. Figure 2: Effect of Drying on Recovery - Oasis® HLB versus C18 Sorbents. No Stopcocks Required! C18 Cartridge (100 mg) Oasis HLB Cartridge (30 mg) ® 100 A Breakthrough in SPE 100 80 80 In October 1977, Waters designed the first miniature cartridge columns (Sep-Pak® cartridges) containing silica-based adsorbents for SPE. New demands for sample preparation led to the development of a new, specially designed polymeric sorbent which performs optimally for reversed-phase SPE. The Oasis® HLB copolymer with unique HydrophilicLipophilic Balance (figure 1) is unlike traditional SPE sorbents. Today's goals for modern solid-phase extraction (SPE) are faster throughput, higher recovery and reproducibility, stronger retention and selectivity. Now SPE can outpace high throughput techniques such as LC/MS/MS. % Recovery 60 Introduction 40 20 % Recovery Procainamide Acetaminophen Ranitidine Propranolol Doxepin 60 40 20 0 0 0 5 10 0 Drying Time (minutes) 4 Oasis® HLB 1cc/30 mg and 1cc/100 mg C18 cartridges were conditioned on a Waters vacuum manifold. When the methanol reached the top of the upper frit in each cartridge, vacuum was maintained for different times to vary the cartridge drying time. The SPE protocol was then continued. The data shown are the average of three replicate extractions. Figure 1: Unique Water-Wettable HLB Copolymer O Figure 3: Higher Retention Means Greater Capacity, No Breakthrough N Retention Factor (k ) Comparison C 18 H O O N N O H Divinylbenzene N O CH 3 N H3 C Acetaminophen OH HO O HO Optimal Properties for Reversed-Phase SPE Specific Surface Area: 810 m2/g Average Pore Diameter: 80 Å Total Pore Volume: 1.3 cm3/g Average Particle Diameter: 30 µm or 60 µm 50 100 Data shown were obtained with two 3.9 mm x 150 mm columns, each packed with one of the sorbents, operated under the same conditions: mobile phase: 20 mM potassium phosphate, pH 7.0/methanol (95/5 v/v); temperature: 30 °C; flow rate: 1.0 mL/min; detection: UV @ 254 nm. © 2001 Waters Corporation 8 Catechol 0 The Oasis® HLB sorbent is a macroporous copolymer made from a balanced ratio of two monomers, the lipophilic divinylbenzene and the hydrophilic N-vinylpyrrolidone. Oasis® HLB Cartridge Theobromine N N CH 3 H N Hydrophilic – Lipophilic Balance Cartridge Salicylic acid HO O O N-Vinylpyrrolidone 8 Drying Time (minutes) Sample Preparation Exceptional Batch-to-Batch Reproducibility Figure 4: General SPE Method (1-D)* The exceptional batch-to-batch reproducibility of the Oasis® sorbent eliminates your need to reserve batches of sorbent for your SPE work. Condition 1 mL methanol Sample Pretreatment Suggestion Applying one or more of the following steps before loading your sample may improve your results: Equilibrate 1 mL water Load 1 mL spiked sample A Simple and Fast Generic (1-D) SPE Protocol for Rapid Method Development • Dilute sample 1:1 with buffer to improve flow during loading Wash 1 mL 5% methanol in water Oasis® HLB sorbent makes it fast and easy to develop sample preparation methods that deliver high, reproducible recoveries especially suited to LC/MS/MS analysis by providing the required selectivity and sensitivity. Unlike silica-based sorbents where you must evaluate several different cartridges, acidic, basic, and neutral compounds, whether polar or nonpolar, can be isolated reproducibly (RSDs <5%) with high recovery (>85%), using the same simple SPE protocol, figures 4 and 5. The generic scheme, figure 4, which has proven useful for a wide variety of compound types may be the only protocol required, reducing method development time. Sample Preparation As a result of careful process design and stringent quality controls, a new standard has been set in batch-to-batch and lot-to-lot reproducibility for SPE methods. • Add 20 µL (H2PO4) to disrupt drug protein interaction Elute 1 mL methanol • Dilute 1:1 or greater with 0.1 N HCL or other acids Evaporate & Reconstitute 40 ˚C/under nitrogen stream 200 µL mobile phase • Filter through 0.45 µm membrane • Centrifuge @ ≥3000 rpm * Volumes are given for the Oasis® HLB cartridge 1cc/30 mg Figure 5: One Simple Procedure: Many Applications on a Universal Sorbent 100 80 sed sorbents With silica-ba ral uld need seve wo u yo ) (C18 ies to achieve istr em ch t en differ n here the results show 60 ■ ■ ■ % Recovery Acids Neutrals Bases n=6 40 RSDs < 3.5% 20 e m er az in e in op Th he eo n br om Pa i n ra za e nth Th i n eo e ph yll in e C af Pr oc fein e ai na m id e Ra ni tid O in e xy co do Pr op ne ra no N lo l al tre x Sa one lb uta m o Do l xe pi n id in ta m Ac e lfa Su Su lfa di az Ac c en Sa lic yli ro x ap Ib N up ro f en 0 Seventeen drugs with a wide range of polarities were spiked in porcine serum, then extracted with 1cc/30 mg Oasis HLB cartridges, using the same SPE method, figure 4. 20 µL/mL H2PO4 was used to disrupt drug-protein binding. ® © 2001 Waters Corporation 9 Sample Preparation Solvent and Sorbent Amount Selection for the Generic Method The amount of sorbent in a cartridge or a plate required to meet your application, is given in Table 1. The solvent used for the elution step should be selected based on the polarity of the analyte. Table 2 gives a selection of elution solvents and each solvent gives you different selectivity and elution strength. Table 1: Capacity and Elution Volume of Oasis® 96-Well Plates and Cartridges Sorbent per device Maximum Mass Capacity Typical Sample Volumes Elution Volume 0.15 to 1 mg 0.35 to 2 mg 1 to 5 mg 2 to 10 mg 10 to 100 µL 50 to 200 µL 100 µL to 1mL 200 µL to 2 mL ≤150 µL ≤250 µL ≥400 µL ≥800 µL 5 mg* 10 mg 30 mg 60 mg * Available only in 96-well plates When converting from C18 silica-based to Oasis® SPE sorbents, use approximately 1⁄3 less Oasis® sorbent. For example 100 mg C18 sorbent = 30 mg Oasis® sorbent Table 2: Tips for Selecting Elution Solvents for the Generic SPE Method (1-D)* The elution solvent is selected based on polarity of analyte. Solvent Solvent Type Relative Elution Strength** Methanol Acetonitrile Tetrahydrofuran Acetone Ethyl Acetate proton donor dipole-dipole dipole-dipole dipole-dipole dipole-dipole 1.0 3.1 3.7 8.8 high Methylene Chloride dipole-dipole high Comments disrupts H-bonding medium polarity drugs medium polarity drugs medium polarity drugs nonpolar drugs and GC compatible nonpolar drugs and GC compatible * When using solvents other than methanol, add 10-30% methanol to disrupt H-bonding on the Oasis® HLB sorbent. ** High-Purity Solvent Guide. Burdick & Jackson Laboratories, Inc. Solvent Properties of Common Liquids, L.R. Snyder, J. Chromatogr., 92, 223 (1974); J. Chromatogr. Sci. 16, 223 (1978) Automation Automation of Oasis® Sample Extraction Cartridges and 96-Well Plates Oasis® SPE products are compatible with the following liquid handling and/or SPE automation systems • Packard MultiProbe II® Robotic liquid handling system Alliance® HT System for LC/MS(MS) • Tomtec Quadra96® and Quadra96® SV Pipetting Station • Control via Micromass MassLynx mass spectrometry software • Hamilton Microlab® SPE Workstation • Beckman Biomek® 2000 Laboratory Automation Workstation • Fast cycle times for high throughput with 96-well plates • Zymark RapidTrace® Automated SPE Workstation • Assay integrity assured with sample manager's temperature control and very low carryover performance • Gilson ASPEC XL4 • Compatibility with the Oasis® on-line extraction columns • Gilson ASPEC XL • Gilson 215 SPE Liquid Handler • Tecan Genesis • Prospekt™/Prospekt™ II © 2001 Waters Corporation 10 Sample Preparation Low Elution Volume and High Throughput 96-Well Extraction Plates Figure 7: Comparisons to Oasis® Low Elution Volume Plate Sample Preparation 100 The Oasis® sorbent, with different amounts in a single 96-well plate, figure 6, gives high and reproducible recoveries for a wide range of analytes including polar and basic drugs, with RSDs ≤ 5% (n=96). Waters offers the standard high-throughput 30 mg and 60 mg Oasis® 96-well plates and has now introduced the 5 mg and 10 mg 96-well low elution plates. 80 % Recovery 60 Eliminate Evaporation Step With Low Elution Volumes Without Sacrificing Capacity 40 2 The high capacity of the Oasis® sorbents makes plasma sample preparation with low elution volumes possible without sacrificing performance, even for highly polar drugs and metabolites, figure 7. Eluting drugs and their metabolites in 150 µL or less volume, eliminates the time consuming and tedious evaporation and reconstitution steps saving hours of assay time. Just elute and shoot. This makes Oasis® products ideally suited for situations where high-throughput SPE is a requirement. 0 Oasis 10 mg Plate ® C18 Disk Plate Procainamide Theophylline Acetaminophen Dipropyl Phthalat 100 Figure 6: Waters 96-Well Plate Design 2000 1R& 9 0D Rin& Aw ar9dD9W10 ner 80 % Recovery 100 Award Two- age Selected as one St of the 100 most technol ogically es W significa nt proel ig duclts D of the yea r n by R&D Magazine 60 40 2 0 Oasis HLB ® Polymer A Polymer B Procainamide 5 mg 10 mg 30 mg 60 mg Ranitidine The Oasis HLB copolymer in the 96-well plate gave significantly higher recovery for polar drugs than two traditional polymeric materials, or a silica-based sorbent. ® By varying frit size and/or placement, the same plate may be filled with various quantities of sorbent per well. Our design permits optimal recoveries, even with low sorbent weights for smaller elution volumes. Low Elution Volume Oasis® HLB Extraction Plate, 5 mg/well, Generic SPE Procedure Condition/Equilibrate 0.5 mL methanol/0.5 mL water Load 100 µL of spiked plasma Wash 1 0.5 mL 5% methanol Elute 100-150 µL 70% acetonitrile/ 30% methanol TM Visit Waters on-line product resource today Dilute 1:1 to 3:1 with water/mobile phase © 2001 Waters Corporation 11 Sample Preparation Selective 2-Dimensional (2-D) Method Development Figure 8: Retention Factor Versus pH for Acids, Bases and Neutrals Meet Selectivity and Sensitivity Goals 20 Theory 18 The generic method can be expanded to meet selectivity and sensitivity goals. A more specific method can be developed to remove plasma or urine interferences. A cleaner extract is achieved by simply manipulating the organic concentration and/or the pH. Acids (HA) 16 B Retention Factor 14 Figure 8 demonstrates how a base compound is ionized at low pH (2 units below its pKa), therefore poorly retained by SPE, and at high pH (2 units above its pKa), strongly retained by reversed-phase SPE. (pKa = 4.8 ) Neutrals 12 (pKa = 9.0 ) 10 8 6 4 A Wash Elute Study is performed to determine the optimal SPE method using organic concentration and pH. A- Bases (BH+) 2 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 pH The 2-D experiment in figure 9 A and B demonstrates this washing, with 70% base modified MeOH in H2O or 30% acid modified MeOH in H2O, will not remove analyte from cartridge. To facilitate complete elution of analyte, an acidified (2% acetic acid in 65% methanol) can be utilized, figure 10. Recommended pH range of Silica Recommended pH range of Oasis® Figure 9A: 2-D Wash Elute Study: Experiment for Oasis® HLB 30 mg 96-well Plate Prepare analytes in saline ▼ From these results, the wash solvent(s) can be either acidic, up to 30% methanol containing 2% acetic acid, or basic, up to 70% methanol containing 2% ammonium hydroxide, since no analyte is eluted under these conditions. To obtain complete elution, the elution solvent should be acidic (2% acetic acid) and the methanol concentration should be greater than 60%. Condition/equilibrate 20 cartridges 1 mL methanol/1 mL water ▼ Load 1 mL spiked saline solution ▼ Elute with 1 mL Peak Area (µVs) 2% NH4OH in MeOH/water Verapamil Norverapamil Methoxyverapamil 0-70% No Response No Response No Response 80% 34,095 47,482 6,358 90% 63,158 85,704 178,178 0% 10% 20% 30% 40% 50% 60% 70% 80% 90% Peak Area (µVs) 0-30% Verapamil Norverapamil Methoxyverapamil No Response No Response No Response 40% 73,146 32,269 86,789 50% 121,820 68,259 137,899 60% 109,145 61,830 123,229 200000 Base Modified Wash 150000 100000 50000 0 Acid Modified Wash 150000 Maximum % Methanol Wash 100000 50000 0 20 40 60 80 % MeOH © 2001 Waters Corporation 12 0% 10% 20% 30% 40% 50% 60% 70% 80% 90% Figure 9B: 2-D Wash Elute Study Results Area (µV*sec) Acid 2% Acetic Acid 2% CH3COOH in MeOH/water Area (µV*sec) Base 2% Ammonium Hydroxide 100 Minimum % Methanol Elution 0 0 20 40 60 % MeOH 80 100 Sample Preparation Selective 2-Dimensional (2-D) Method Development (continued) Figure 10: 2-D SPE Method Developed from Wash Elute Study utral Removes ne d basic non-polar an erferences lipophilic int Equilibrate 1 mL water Wash 3 1 mL 65% MeOH in water with 2% ammonium hydroxide Load 1 mL spiked sample sic Elute the ba in compounds ionized form utral Removes ne sic polar and ba ences polar interfer Wash 1 1 mL 5% MeOH in water with 2% acetic acid idic Removes ac ences polar interfer Wash 2 1 mL 5% MeOH in water with 2% ammonium hydroxide Elute 1 mL 65% MeOH in water with 2% acetic acid Evaporate & Reconstitute 40 ˚C/under nitrogen stream 200 µL water/mobile phase Figure 11: Better Results with a 2-Dimensional Method 0.004 AU 1 High 2 AV 100% pH 3 Sample 3 2 Column: 5% Blank 1 Low Low Organic Concentration High 2-D Method with 2 washes AV 1 High 2 0.004 AU 3 65% 2 pH 3 Sample Blank 100% SymmetryShield™ RP8, 5 µm, 3.9 x 150 mm Temperature: 30 °C Mobile Phase: 50 mM Phosphate pH 7: acetonitrile:methanol (41:37:22); Alliance® 2690 Detection: UV at 230 nm Flow Rate: 1.0 mL/min. Injection Volume: 40 µL (after evaporation and reconstitution in 200 µL water) Peak Identification: Peak 1: Norverapamil Peak 2: Verapamil Peak 3: Methoxyverapamil (I.S.) 5% 1 4 Low Low Organic Concentration High 2-D Method with 3 washes 2 1 High 3 0.004 AU AV 3 pH 1 Low Low 0 2 4 6 8 100% 5% Sample Blank 4 65% 2 5 Organic Concentration High 10 Minutes © 2001 Waters Corporation 13 Sample Preparation Condition 1 mL methanol A cleaner extract was achieved for verapamil and its metabolite, figure 11, using the 2-D procedure, figure 10, instead of the generic method in figure 4. The more selective method enables higher sensitivity for analyses at ng/mL levels. Sample Preparation Oasis® MCX plates, cartridges and columns Figure 13: Oasis® MCX Method for Extraction of Basic Compounds ning No conditio Load 3 mL spiked and acidified urine Wash 11 Wash 2 mL 0.1N HCI utral and Removes ne ences acidic interfer Wash 2 2 mL methanol Elute B 2 mL 5% ammonium hydroxide in methanol Evaporate and Reconstitute 300 µL 20% methanol in water Oasis® MCX 3 cc/60 mg cartridge High Selectivity and Sensitivity for Basic Compounds With No Conditioning of the Oasis® MCX Sorbent Figure 14: Comparison of Oasis® MCX Cartridges to other SPE Silica-Based Products Using the Generic Oasis® MCX Method 100 Obtain selective retention of basic drugs with cation-exchange groups. The Oasis® MCX sorbent has a tightly controlled ion-exchange capacity (1 meq/gram), figure 12. There are no silanol groups to complicate the retention mode or method development. This novel, water-wettable, polymeric sorbent is stable from pH 0 to 14, making method development simple and fast. Methadone 40 Propranolol 20 0 The use of a pH modified wash system allows optimization of the method and it produces a very selective protocol for basic compounds, figure 15. 13% 12% 1.1% Methadone metabolite (EDDP)* 2.4% 3.2% 3.2% As shown in figure 13, one protocol with minimum wash steps gives extractions fast enough to keep pace with your analytical system. No conditioning or equilibration of cartridges is required to achieve excellent results for basic drugs, see figure 14. 60 0.3% 0.7% 0.5% % Recovery (% RSD) 80 Oasis® MCX 60 mg/3 cc Brand A 300 mg/ 3 cc Brand B 300 mg/ 3 cc Figure 15: Selectively Separate Primary and Secondary Amines from Tertiary and Aromatic Amines Using an Oasis® MCX Cartridge Figure 12: Oasis® MCX Sorbent 100 O N Toluidine (Aromatic) 75 Doxepin ( 3˚) Amitriptyline ( 3˚) SO3 H SO3 H Procainamide ( 3˚) 85 Elute tertiary and aromatic amines with 5% TEA 50 Elute primary and secondary amines with 5% NH4OH in methanol 25 HO3S N 0 Mixed-Mode, Cation-Exchange Reversed-Phase Sorbent N-methylbenzylamine ( 2˚) Benzylamine (1˚) Methamphetamine ( 2˚) 0 200 400 600 800 Elution Volume (µL of 5% TEA in Methanol) © 2001 Waters Corporation 14 N,N-dimethylbenzylamine ( 3˚) Amphetamine (1˚) 15 O Nortriptyline ( 2˚) 1000 Sample Preparation Oasis® MAX plates, cartridges and columns Figure 16: Oasis® MAX Sorbent Sample Preparation O N + CH2 NR 3 + CH 2NR3 N + R3NCH2 O Mixed-Mode, Anion-Exchange Reversed-Phase Sorbent Figure 17: Generic Oasis® MAX Method for Extraction of Acidic Drugs High Selectivity and Sensitivity for Acidic Compounds using the Oasis® MAX Sorbent Condition 1 mL methanol The Oasis MAX sorbent, figure 16, has a tightly controlled ion exchange capacity of 0.3 meq/gram ensuring reproducible SPE protocols for extraction of acidic compounds and metabolites from biological fluids. ® This novel, water wettable polymeric sorbent is stable from pH 0 to 14 making method development simple and fast, figure 17. As shown in figure18, acidic compounds can be separated from basic and neutral compounds on the same Oasis® MAX cartridge. sic and Removes ba ferences neutral inter Equilibrate 1 mL water Wash 2 (Elute 1) 2 mL methanol Load 3 mL sample Elute 2 2 mL 100 mM phosphoric acid/acetonitrile 1:1 (v/v) Wash 2 mL 50 mM sodium acetate pH 7/5% methanol Alternative LC/MS Elution System: 2% formic acid in methanol Figure 18: High Recovery of Acidic, Basic and Neutral Compounds Using the Generic Oasis® MAX Method Elute 1 (basic and neutral drugs) Elute 2 (acidic drugs) 1 2 I.S. I.S. AV AV 0 2 4 6 8 10 Sample Sample Blank with I.S. Blank with I.S. 0 2 Minutes Column: 4 3 SymmetryShield™ 3.5 µm, 4.6 mm x 100 mm SymmetryShield™ Guard column, 5 µm, 3.9 mm x 20 mm Mobile Phase: 20 mM potassium phosphate, pH 2.7/methanol, 52:48 v/v Detector: UV @ 214 nm Temperature: 30 °C Flow Rate: 2 mL/min Injection Volume: 10 µL of extract with internal standard 4 6 8 10 Minutes Peak Identification 1. Nortriptyline 2. Secobarbital 3. Ketoprofen 4. Naproxen I.S. Butylparaben Results (n=6) Drugs Nortriptyline Secobarbital Ketoprofen Naproxen µg/mL Type of Drug % Recovery % RSD 5 10 5 2.5 Basic (Elute 1) Neutral (Elute 1) Acidic (Elute 2) Acidic (Elute 2) 94.34 88.53 91.64 103.43 0.78 1.03 2.35 3.04 1 mL urine hydrolyzed (1M KOH 60 °C, 15 minutes) and adjusted to pH 2 with phosphoric acid; diluted 1:1 with 10 mM sodium acetate, pH 2. © 2001 Waters Corporation 15 Sample Preparation Ultrafast On-Line SPE With Oasis® Extraction Columns for LC/MS/MS Details of the technique Ultrafast chromatography is a robust and straightforward technique that combines HPLC and mass spectrometry. A diluted plasma sample is injected onto the Oasis® HLB 1 mm x 50 mm column. The column is then purged of most protein and interferences. A fast gradient is run to elute the target analyte and one tenth of this flow (400 µL/min) is diverted to the mass spectrometer for detection, figure 18. The Oasis® HLB 2.1 mm x 20 mm cartridge column is very rugged and gives even more robust assays and higher sensitivity. Up to 360 injections were achieved on a single column without any visible deterioration of the peak shape, figure 20. Oasis® Cartridge Column 2.1 mm x 20 mm Figure 18: Ultrafast, on-line chromatography Oasis® HLB cartridge column 1 mm x 50 mm PUMP Ultrafast on-line analysis of multiple analytes can be accomplished using the Oasis® extraction column, combined with a traditional analytical column, as shown in figure 19. Waste Switching Valve INJ 4 mL/min Flow Splitter 10:1 Oasis® Column 1 mm x 50 mm 400 µL/min MS/MS Selected Reaction Monitoring Ayrton, J, Dear GJ, Leavens WJ, Mallett DN, Plumb RS. Optimization and routine use of generic ultra-high flow-rate liquid chromatography with mass spectromic detection for the direct on-line analysis of pharmaceuticals in plasma. J Chromatogr A. 1998 Dec 18;828(1-2):199-207 Figure 19: Fast on-line analysis: Multiple drugs with dual columns Oasis® HLB cartridge column 2.1 mm x 20 mm PUMP Figure 20: Column Lifetime: Overlay of Toluamide after 360 Plasma Injections Waste Column: Mobile Phase: Switching Valve INJ Gradient: 3 mL/min Symmetry C18 5 mm 3.9 mm x 150 mm ® 0.5 mL/min Detector: Injection Vol. Total Run Time 5 min Back-pressure: MS/MS Selected Reaction Monitoring M. Jemal, M., et., Rapid Comm Mass Spec 1998, 12 pp 1389-1399 A switching valve is needed to divert the HPLC void volume to waste in order to prevent proteins and other polar matrix interferences from entering the MS source, thereby enhancing the MS performance. 0.0 © 2001 Waters Corporation 16 1.0 Minutes Oasis® HLB cartridge column, 2.1 mm x 20 mm; A = 100% 0.1% (aqueous) formic acid, B = 95% Acetonitrile:5% aqueous with 0.1% formic acid in both Flow Rate: 4 mL/min 0 to 0.2 min 100% A 0.2 to 0.8 min 100% B Re-equilibrate 0.2 min at 100% A Using splitter 0.4 mL/min to mass spectrometer 50 µL of dilute plasma (1:1 with mobile phase A) Constant throughout the study Sample Preparation On-Line SPE With Oasis® HLB Sorbent in Prospekt™ Cartridges Sample Preparation The Oasis® HLB sorbent in Prospekt™ cartridges shows better recoveries for pharmaceutical compounds than silica-based sorbents, figure 21. Oasis® HLB Prospekt™ cartridges give you rapid method development, simpler methods, and equivalent or higher recoveries than previously achieved even for the most challenging polar compounds. Figure 21: Oasis® HLB Sorbent Superior to C18-Bonded Silica Oasis HLB ® C18 C2 0 Available Soon ™ Prospekt II Cartridges 20 Clonidine 40 60 80 100 Terconazole The general method in Figure 4 was used with 15% acetonitrile wash and 55% acetonitrile for elution to the analytical column. * Data were provided by Dr. Francis Beaudry, a Principal Research Scientist of Phoenix International Life Sciences Inc. in Montreal, Quebec, Canada. Oasis® Glass Cartridges for PPT Detection Levels Waters Oasis® glass cartridges are available in 5 cc (200 mg) configuration with Teflon® frits for trace analysis at parts per trillion level. Each lot is tested for the presence of bisphenol A and other phenols and phthalates, assuring that endocrine disruptors in water samples can be analyzed to part per trillion levels. See the full application in the Oasis® Environ-mental & Agrochemical Applications Notebook (Waters Literature Code #WN037). PPT Recovery of Estrogens from River Water, LC/MS, 5 ng/L Spike Level, n=4 Results 1. bisphenol A 2. 17β-estradiol 3. 17α-ethynylestradiol 4. estrone 5. diethylstilbestrol Ions Monitored (m/z) Recovery % RSD 227 271 295 269 267 113 93 96 87 75 11 15 12 5 5 Recovery of Phthalates and Nonylphenol from River Water, GC/MS, 200 ng/L Spike Level, n=4 Results 1. dimethylphthalate 2. dirthylphthalate 3. n-nonylphenol 4. dibutylphthalate 5. benzylbutylphthalate 6. bis(ethylhexyl)phthalate ISTD. o-terphenyl (internal standard) Recovery % RSD 130 86 90 110 110 60 15 12 11 11 8 8 © 2001 Waters Corporation 17 Sample Preparation Oasis® Cartridge Selection Guide 1 cc/10 mg 1 cc/30 mg 1 cc/30 mg Gilson Adapter 3 cc/60 mg 3 cc/60 mg Gilson Adapter 6 cc/150 mg 6 cc/200 mg Box of 100 Box of 100 Box of 500 Box of 100 Box of 500 Box of 30 Box of 30 Oasis® HLB 30 µm 186000383 WAT094225 WAT058882 WAT094226 WAT058883 N/A WAT106202 Oasis® HLB 60 µm N/A N/A N/A N/A N/A N/A N/A Oasis® MCX 30 µm N/A 186000252 N/A 186000254 N/A 186000256 N/A Oasis® MCX 60 µm N/A 186000782 186000782 186000253 N/A 186000255 N/A Oasis® MAX 30 µm N/A 186000366 N/A 186000367 N/A 186000369 N/A Oasis® MAX 60 µm N/A N/A N/A 186000368 N/A 186000376 N/A 5 mg/96-well 10 mg/96-well 30 mg/96-well 60 mg/96-well 1/Pkg 1/Pkg 1/Pkg 1/Pkg Oasis® HLB 30 µm 186000309 186000128 WAT058951 N/A Oasis® HLB 60 µm N/A N/A N/A 186000679 Oasis® MCX 30 µm N/A 186000259 186000248 Oasis® MCX 60 µm N/A N/A 186000250 186000678 Oasis® MAX 30 µm N/A 186000375 186000373 N/A Oasis® MAX 60 µm N/A N/A N/A N/A Sorbent Oasis® 96-well Plates © 2001 Waters Corporation 18 Sample Preparation 12 cc/500 mg 20 cc/1 g 35 cc/6 g Plus 225 mg Vac RC 30 mg Vac RC 60 mg Sample Preparation 6 cc/500 mg Glass Cartridge 5 cc/200 mg NEW Box of 30 Box of 20 Box of 20 Box of 10 Box of 50 Box of 50 Box of 50 Box of 30 N/A N/A N/A N/A N/A 186000382 186000381 N/A 186000115 186000116 186000117 186000118 186000132 N/A N/A N/A N/A N/A N/A N/A N/A 186000261 N/A 186000776 N/A 186000777 186000778 N/A N/A 186000380 N/A N/A N/A N/A N/A N/A 186000372 186000371 N/A 186000865 N/A N/A N/A N/A N/A 186000378 N/A NEW 186000683 For the latest Oasis® applications visit www.waters.com, choose HPLC Columns and Supplies, then click on Sample Preparation. Download the latest applications, or search our online Applications Library containing thousands more applications covering the entire range of Waters products. If you do not have internet access, wish to receive printed copies, or require further information, contact your local Waters Sales Office. © 2001 Waters Corporation 19 Sample Preparation Oasis® HLB Sample Extraction Products Description Particle Size Quantity Part Number Price Oasis HLB cartridge Oasis® HLB cartridge Oasis® HLB cartridge with Gilson ASPC™ Adapter Oasis® HLB cartridge Oasis® HLB cartridge with Gilson ASPC™ Adapter Oasis® HLB cartridge Oasis® HLB cartridge Oasis® HLB cartridge Oasis® HLB cartridge Oasis® HLB cartridge Oasis® HLB Plus cartridge Oasis® HLB Vac RC cartridge Oasis® HLB Vac RC cartridge Oasis® HLB Glass cartridge Adapter, 5 cc, Teflon® Oasis® HLB Prospekt™ cartridge* 1 cc/10 mg 1 cc/30 mg 1 cc/30 mg 3 cc/60 mg 3 cc/60 mg 6 cc/200 mg 6 cc/500 mg 12 cc/500 mg 20 cc/1 g 35 cc/6 g 225 mg 30 mg 60 mg 5 cc/200 mg 5 cc 2 mm x 10 mm/15 mg 30 µm 30 µm 30 µm 30 µm 30 µm 30 µm 60 µm 60 µm 60 µm 60 µm 60 µm 30 µm 30 µm 60 µm – 30 µm 100/box 100/box 500/box 100/box 500/box 30/box 30/box 20/box 20/box 10/box 50/box 50/box 50/box 30/box 10/box 100/box 186000383 WAT094225 WAT058882 WAT094226 WAT058883 WAT106202 186000115 186000116 186000117 186000118 186000132 186000382 186000381 186000683 405000934 186000258 ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? Oasis® HLB column Oasis® HLB cartridge column Holder Kit for 2.1 mm x 20 mm cartridge column In-Line pre-column filter kit 1 mm x 50 mm 2.1 mm x 20 mm 30 µm 25 µm 1/box 1/box 1/box 1/box 186000119 186000706 186000262 WAT084560 ??? ??? ??? ??? 5 mg/96-well 10 mg/96-well 30 mg/96-well 60 mg/96-well 30 µm 30 µm 30 µm 60 µm 1/pkg 1/pkg 1/pkg 1/pkg 186000309 186000128 WAT058951 186000679 ??? ??? ??? ??? ® Oasis® HLB plate Oasis® HLB plate Oasis® HLB plate Oasis® HLB plate * For use with Spark Holland Prospekt™ system Oasis® MCX Sample Extraction Products (Cation-Exchanger) Description Oasis® MCX cartridge Oasis® MCX cartridge Oasis® MCX cartridge Oasis® MCX cartridge Oasis® MCX cartridge Oasis® MCX cartridge Oasis® MCX cartridge Oasis® MCX cartridge Oasis® MCX cartridge Oasis® MCX cartridge Oasis® MCX cartridge Oasis® MCX cartridge Oasis® MCX Vac RC cartridge Oasis® MCX Vac RC cartridge Oasis® MCX plate Oasis® MCX plate Oasis® MCX plate Oasis® MCX Plate Particle Size Quantity Part Number Price 1 cc/30 mg 1 cc/30 mg LP** 3 cc/60 mg 3 cc/60 mg 6 cc/150 mg 6 cc/150 mg 6 cc/500 mg 20 cc/1 g 35 cc/6 g 6 cc/500 mg LP** 20 cc/1 g LP** 35 cc/6 g 60 mg 60 mg 30 µm 60 µm 30 µm 60 µm 30 µm 60 µm 60 µm 60 µm 60 µm 60 µm 60 µm 60 µm 30 µm 60 µm 100/box 100/box 100/box 100/box 30/box 30/box 30/box 20/box 10/box 30/box 20/box 10/box 50/box 50/box 186000252 186000782 186000254 186000253 186000256 186000255 186000776 186000777 186000778 186000776 186000777 186000778 186000261 186000380 ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? 10 mg/96-well 30 mg/96-well 30 mg/96-well 60 mg/96-well 30 µm 30 µm 60 µm 60 µm 1/pkg 1/pkg 1/pkg 1/pkg 186000259 186000248 186000250 186000678 ??? ??? ??? ??? ** LP = Large Pore © 2001 Waters Corporation 20 Sample Preparation Oasis® MAX Sample Extraction Products (Anion-Exchanger) Description Particle Size Quantity Part Number Price 1 cc/30 mg 3 cc/60 mg 3 cc/60 mg 6 cc/150 mg 6 cc/150 mg 6 cc/500 mg 30 mg 60 mg 60 mg 30 µm 30 µm 60 µm 30 µm 60 µm 60 µm 30 µm 30 µm 60 µm 100/box 100/box 100/box 30/box 30/box 30/box 50/box 50/box 50/box 186000366 186000367 186000368 186000369 186000370 186000865 186000372 186000371 186000378 ??? ??? ??? ??? ??? ??? ??? ??? ??? Oasis® MAX plate Oasis® MAX plate 10 mg/96-well 30 mg/96-well 30 µm 30 µm 1/pkg 1/pkg 186000375 186000373 ??? ??? Particle Size Quantity Part Number Price 30 µm 10 each HLB, MCX, MAX 186000867 ??? Sample Preparation Oasis MAX cartridge Oasis® MAX cartridge Oasis® MAX cartridge Oasis® MAX cartridge Oasis® MAX cartridge Oasis® MAX cartridge Oasis® MAX Vac RC cartridge Oasis® MAX Vac RC cartridge Oasis® MAX Vac RC cartridge ® NEW Oasis® Method Development Kit Description Oasis® Method Development Kit 3 cc/60 mg Manifold for Extraction Plate Description Extraction Plate Manifold Kit A (includes extraction plate manifold, reservoir tray, manifold top gasket, sealing cap and 350 µL sample collection plate) Extraction Plate Manifold Kit B (includes extraction plate manifold, reservoir tray, manifold top gasket, sealing cap and 1 mL sample collection plate) Extraction Plate Manifold Kit C (includes extraction plate manifold, reservoir tray, manifold top gasket, sealing cap and 2 mL sample collection plate) Extraction plate manifold Accessories for Extraction Cartridges Part No. Price WAT097944 ??? WAT097945 ??? Waters extraction manifold, 20-position without rack (includes 20 needle tips, 25 plugs, and ejector tool) Waters extraction manifold, 20-position (complete with rack for 13 mm x 75 mm tubes) Waters extraction manifold, 20-position (complete with rack for 13 mm x 100 mm tubes) Waters extraction manifold, 20-position (complete with rack for 16 mm x 75 mm tubes) Waters extraction manifold, 20-position (complete with rack for 16 mm x 100 mm tubes) Vacuum pump (110V, 60 Hz) Vacuum pump (220V, 50 Hz) Vacuum pump (110V, 50 Hz) Reservoir 30 cc for Oasis cartridges Reservoir 60 cc for Oasis® cartridges Reservoir Adapter for 12, 20 and 35 cc cartridges ® Quantity Part No. Price 48/box 12/box 10/box WAT011390 WAT024659 WAT048160 ??? ??? ??? Quantity Part No. Price 25/box 50/box 50/box 50/box 50 sheets/pkg 1/pkg 1/pkg WAT058942 WAT058943 WAT058957 WAT058958 WAT058959 WAT058955 WAT058956 ??? ??? ??? ??? ??? ??? ??? Accessories for Extraction Plate Manifold WAT097946 ??? WAT058941 ??? Part No. Price WAT200677 ??? WAT200606 ??? WAT200607 ??? WAT200608 ??? WAT200609 WAT085114 WAT085115 WAT085123 ??? ??? ??? ??? Manifold for Extraction Cartridges* Description Description Description Reservoir tray Sample collection plate, 350 µL Sample collection plate, 1 mL Sample collection plate, 2 mL Sealing cap for 96-well collection plate Manifold gasket, top Manifold gasket, white Waters Oasis® HLB Extraction Plate 30 mg/Well, 96-Wells/Plate WAT058951 WAT058939 (1 Plate/Box) (3 Plates/Box) Sample Collection Plate Polypropylene, 96-Wells, 50 Plates/Pkg WAT058943 WAT058957 WAT058958 350 µL 1 mL 2 mL Manifold Waste Tray (Disposable) WAT058942 25/Pkg Top Extraction Plate Manifold WAT058941 Bottom © 2001 Waters Corporation 21 Sample Preparation Sep-Pak® DNPH-Silica Cartridges for Analyzing Formaldehyde, Other Aldehydes and Ketones in Air Formaldehyde and other aldehydes are receiving increasing attention both as toxic substances and as promoters in the photochemical formation of ozone in air. Source of aldehydes in residential buildings include plywood and particle board, insulation, combustion appliances, tobacco smoke and various consumer products. Aldehydes are released into the atmosphere in the exhaust of motor vehicles and other equipment in which hydrocarbon fuels are incompletely burned. Figure 6: HPLC Separation of DNPH Derivatives of Common Aldehydes and Ketones Column: Sample: Mobile Phase: Gradient: Flow Rate: Injection: Detection: Nova-Pak® C18 3.9 mm x 150 mm Mixture of DNPH and DNPH derivatives in acetonitrile. A: Water/Acetonitrile/Tetrahydrofuran 60/30/10 v/v/v B: Water/Acetonitrile 40/60 v/v 3mL 100% A for 1 minute, then linear gradient from 100% A to 100% B in 10 minutes 1.5 mL/min 20 µL 360 nm The most sensitive and specific method for analyzing aldehydes and ketones is based on their reaction with 2,4-dinitrophenylhydrazine (DNPH) and subsequent analysis of the hydrazone derivatives by HPLC (see Figure 6). The hydrazones may be detected by absorbance in the ultraviolet region, with maximum sensitivity obtained between 350 and 380 nm. Airborne aldehydes have traditionally been collected by drawing a sample through an impinger containing a solution of DNPH. However, the impinger collector is generally cumbersome to use and is not well suited for high flow rates or extended collection times due to solvent evaporation. The new Sep-Pak® DNPH-Silica cartridges meet the requirements of EPA Method TO-11 and provide a convenient device for sample collection. Using a vacuum pump, an air sample is drawn through the new SepPak® DNPH-Silica cartridge. The aldehydes and ketones react with the DNPH and form the hydrazone derivative, which is retained on the cartridge. Later, the hydrazones are eluted from the cartridge with acetonitrile and analyzed by HPLC. Detection limits can be as low as 3 ppbv for a 100 liter sample. Advantages of Waters Sep-Pak® DNPH-Silica Cartridges These cartridges provide you with significant advantages when compared to other techniques, such as liquid impingers, for the analysis of aldehydes and ketones. In addition, a new high speed, high resolution HPLC application has been developed to provide excellent quantitation capability in the low parts-per-billion range (see Figures 7 and 8). • Sep-Pak DNPH-Silica cartridges meet the requirements of EPA Method TO-11 and ASTM-D-5791-1. ® • Results from impingers and these cartridges are in excellent agreement. DNPH Formaldehyde-DNPH Acetaldehyde-DNPH Acetone-DNPH Acrolein-DNPH Propionaldehyde-DNPH Crotonaldehyde-DNPH Butanone-DNPH Butyraldehyde-DNPH Benzaldehyde-DNPH Isovaleraldehyde-DNPH Valeraldehyde-DNPH o-Tolualdehyde-DNPH m-Tolualdehyde-DNPH p-Tolualdehyde-DNPH Hexaldehyde-DNPH 2,5-Dimethylbenzaldehyde-DNPH Figure 7: Low-Level Example: Aldehyde Profile from Laboratory Air Lab Air Sample 1. 2. 3. 4. 5. 6. DNPH Formaldehyde-DNPH Acetaldehyde-DNPH Acetone-DNPH Butanone-DNPH Isovaleraldehyde-DNPH Cartridge Blank (calculated for 100 liter sample) 1. 2. 3. 4. 4.8 ppbv 1.2 ppbv 118.0 ppbv 0.8 ppbv 0.7 ppbv DNPH Formaldehyde-DNPH Acetaldehyde-DNPH Acetone-DNPH 0.35 ppbv 0.27 ppbv 0.34 ppbv The sample of Figure 7 was obtained in a chemical research laboratory using a portable sampling pump. One hundred liters of air was drawn through the cartridge at 0.65 L/min. The sample was found to contain low concentration of formaldehyde (4.8 ppbv), acetaldehyde (1.2 ppbv) and methyl ethyl ketone (0.8 ppbv), but a significant amount of acetone (118 ppbv). Significant concentrations of acetone are frequently found in laboratories because of its widespread use as a solvent. Figure 8: High-Level Example: Aldehyde Profile from Diluted Auto Exhaust Emissions Sample (8.4 L at 1 L/min) collected at US EPA Atmospheric Research Assessment Lab. Courtesy of Dr. S. B. Tejada. • Solvent consumption, solvent exposure and hazardous waste disposal costs are reduced. • Sep-Pak® DNPH-Silica cartridges provide superior convenience and reproducibility, making them ideal for field sampling and process monitoring applications. • Sep-Pak® DNPH-Silica cartridges can save time and increase productivity. • Increased safety. © 2001 Waters Corporation 22 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. DNPH Formaldehyde-DNPH Acetaldehyde-DNPH Acetone-DNPH Acrolein-DNPH Propionaldehyde-DNPH Crotonaldehyde-DNPH 2-Butanone-DNPH Butyraldehyde-DNPH Benzaldehyde-DNPH o-Tolualdehyde-DNPH m-p-Tolualdehyde-DNPH 2,5-Dimethylbenzaldehyde-DNPH 2290 ppbv 367 ppbv 26 ppbv 8 ppbv 12 ppbv 36 ppbv 24 ppbv 15 ppbv 32 ppbv 5 ppbv 11 ppbv 3 ppbv Sample Preparation Waters XPoSure™ Aldehyde Sampler Cartridges for Monitoring Aldehydes in indoor air. Sample Preparation Based on an extension of our DNPH coating technology, XPoSure™ Aldehyde Sampler cartridges are the most sensitive active samplers available today. Figure 9: Low-Level Example: Aldehyde Profile from Laboratory Air Highest Sensitivity 4 1 Compared to existing sampling tube technology which have high and variable backgrounds, XPoSure™ cartridges are guaranteed to give consistent low aldehyde backgrounds, cartridge-to-cartridge, lot-to-lot. 1. 2. 3. 4. 5. 6. DNPH Formaldehyde-DNPH Acetalhyde-DNPH Acetone-DNPH Butanone-DNPH Isovaleraldehyde-DNPH High-Collection Efficiencies You can achieve > 95% collection efficiencies for all aldehydes at flows of up to a liter per minute. And, you only need to use one cartridge - no breakthrough bed is necessary. 2 3 Low Pressure Drop - Use with Portable Personal Sampling Pumps 5 6 Lab Air Sample Large particle size and higher porosity frits make this new XPoSure cartridge compatible with personal sampling pumps. ™ Easy-to-Use Cartridge Blank 0 Sample, elute and shoot, it’s that easy. You’ll never have to break open and manipulate a glass tube again. And because the cartridges are made from high density polyethylene (HDPE), breakage is not a concern. 2 4 6 8 Time (minutes) 10 12 14 The above sample was collected in a chemical research laboratory using a portable sampling pump. One hundred liters of air was drawn through the cartridge at 0.65 L/min. The chromatogram shows ormaldehyde (4.8 ppbv), acetaldehyde (1.2 ppbv), acetone (118 ppbv), butanone (0.8 ppbv), and isovaleraldehyde (0.7 ppbv). The figure below shows two traces. An actual cartridge blank demonstrating extremely low background levels and as an actual laboratory air sample. Ozone Scrubber Cartridge Ozone has been shown to interfere with the analysis of carbonyl compounds in air samples that have been drawn through cartridges containing silica-coated with Flow Schematic for Air Sampling System 2,4-dinitrophenylhydrazine (DNPH). Waters Ozone Scrubber cartridges are designed to remove this ozone interference. These disposable devices are intended for use in series combination with the Waters Sep-Pak® DNPH-Silica cartridges or XPoSure™ Aldehyde Sampler cartridges. One Ozone Scrubber cartridge replaces the 1⁄4” diameter by 36” long copper ozone denuder located in the heated zone of sampling systems used for outdoor air monitoring (PAMS program). Air Sample In O3 Scrubber Cartridge DNPH-Silica or XPoSure™ Cartridge Vacuum Sampling Pump Each Ozone Scrubber cartridge contains 1.4 grams of granular potassium iodide. When air containing ozone is drawn through this packed bed, iodide is oxidized to iodine, consuming the ozone, according to the following reaction: The theoretical capacity of a single cartridge is 4.2 mmoles of ozone (200 mg). The particle size of the potassium iodide granules is optimized for good mass transfer and flow characteristics. © 2001 Waters Corporation 23 Sample Preparation Porapak® RDX Sep-Pak® Extraction Cartridge for the Analysis of Explosives in Surface and Ground Waters. Designed to meet or exceed the QA/QC requirements of EPA method 8330, it is ideal for environmental testing laboratories supporting Department of Defense remediation programs. High Sensitivity Porapak® Sep-Pak® cartridges contain Porapak® RDX resin, a specially prepared, specially cleaned divinylbenzene/vinylpyrrolidone copolymer, packed in a high purity polyethylene syringe barrel. With the lowest guaranteed backgrounds and the highest cartridge-to-cartridge, lot-to-lot consistency, The Waters Porapak® RDX column is the most sensitive technology available today and allows you to perform analysis at sub ppb levels. Increase Productivity and Reduce waste By using Porapak® RDX cartridges, you can reduce the amount of organic solvent used per sample by 10-fold and decrease your sample prep time by 3x. Activate, Load, Elute and Shoot Flow 15 mL acetonitrile through cartridge at 10 mL/min Flow 30 mL water through cartridge at 10 mL/min Load 500 mL water sample at 10 mL/min Elute with 5 mL acetonitrile Unmatched Recoveries The specially prepared resin is highly selective for nitroaromatic and nitramine compounds, resulting in recoveries of 90% or greater. Dilute to 40% acetonitrile with HPLC grade water Inject 100 µL onto HPLC Recovery data from preconcentrating 500 mL of explosives standards in sterile water at two concentrations on Porapak® RDX Sep-Pak® Vac columns. Number of replicates = 7. 1 ppb % Recovery % RSD 10 ppb % Recovery % RSD HMX TNB RDX 100.5 95.9 90.9 6.7 3.5 6.4 100.5 99.3 98.7 3.9 3.3 3.2 DNB TNT TETRYL 99.5 97.0 89.0 3.2 3.0 6.4 99.2 102. 102.8 3.2 3.7 4.7 NB 35DNA 24DNT 96.5 91.2 97.3 2.5 3.3 3.4 97.9 98.2 99.9 2.8 3.6 3.4 26DNT 2A 4A 94.5 92.4 90.0 3.4 5.2 4.9 98.7 98.0 97.2 3.4 3.7 4.1 4NT 2NT 89.5 96.8 4.3 6.6 100.4 93.4 3.7 3.0 Compound Figure 10: Isocratic Separation of Method 8330 Analytes Column: Mobile Phase: Flow rate: Injection volume: Temperature: Detection: Sample: Waters Nova-Pak® C8, 3.9 x 150 mm 82:18 water/isopropanol 1.0 mL/min 100 mL 24°C UV @ 254 nm 1 ppb explosives concentrated 40-fold on Porapak® RDX, in 60:40 water/acetonitrile Achieve the lowest method detection limits using Waters Porapak® RDX cartridges. The top chromatogram shows the analysis of reagent H2O spiked at the 1 ppb level. The bottom chromatogram is a reagent (H2O) blank. © 2001 Waters Corporation 24 Sample Preparation Sep-Pak® DNPH-Silica Cartridge XPosure™ Aldehyde Sampler Cartridge Quantity Part No. Price Sep-Pak® DNPH-Silica Cartridge Box of 20 WAT037500 195.00 Ozone Scrubber Description Description Quantity Part No. Price XPoSure™ Aldehyde Sampler Cartridge Box of 20 WATO47205 195.00 Porapak® RDX Cartridges and Accessories Quantity Part No. Price Description Quantity Porapak RDX Cartridges Box of 30 Tubing, Tefzel, 1/8 inch O.D. x 0.040-inch I.D. Sep-Pak® Vac Adapter Box of 12 60 cc Sep-Pak Reservoir Box of 12 Male-Male Adapter ® Ozone Scrubber Sample Preparation Description Box of 20 WATO54420 105.00 Part No. Price WAT047220 WAT023344 WAT054260 WAT024659 WAT024310 180.00 68.00 35.00 12.00 55.00 Sep-Pak® C18 Environmental Cartridges Description Quantity Part No. Price C18 Environmental Cartridge tC18 Environmental Cartridge Box of 50 Box of 50 WAT023635 WAT036800 168.00 168.00 TM Visit Waters on-line product resource today © 2001 Waters Corporation 25 Sample Preparation Waters Solid-Phase Extraction Applications Guide and Bibliography, 6th Edition. A resource for Sample Preparation Methods Development. Designed to be easy-to-use, the 600 page bibliography has separate indexes by: Compound Now make your methods development effort easier with references to over 3,000 published papers utilizing Sep-Pak® cartridges in sample preparation protocols. The 6th Edition bibliography has more than 600 pages, contains 3,000 plus referenced papers, more than 8,000 compound entries, 8,000 author entries and more than 1,000 sample matrix entries. It also has an expanded sample preparation primer to assist in your development of a sample preparation protocol. Sample Matrix Author To help you in your methods development, see information on Sep-Pak® Plus Methods Development Kits below. Paper reference file number (numerical sequence) Publication (page reference) Application category Sample matrix Title Cartridge selection Compound(s) Analytical technique Author(s) Now Online - Click Here to Search Waters Solid-Phase Extraction Applications Guide and Bibliography, 6th Edition. Solid-Phase Extraction Technology Training Video Description Part No. Price Waters Solid-Phase Extraction Applications Guide and Bibliography, 6th Edition WAT052853 75.00 Experiments in Chromatography: Solid-Phase Extraction Technology, an 18 minute video designed for use in the training of laboratory personnel and chemistry students. Description Experiments in Chromatography Experiments in Chromatography Sep-Pak® Plus Methods Development Kits Description Quantity Methods Development Kit A Box 100 (contains 10 Sep-Pak® cartridges each of Alumina: Acid, Basic, Neutral, CN, Diol, Silica, Florisil, Accell Plus QMA, C18, and NH2) Methods Development Kit B Box 50 (contains 10 Sep-Pak® cartridges each of NH2, CN, Diol, Silica and C18) Methods Development Kit C Box 50 (contains 10 Sep-Pak® cartridges each of Accell Plus QMA, Accell Plus CM, C18, Silica and Diol) Part No. Price WAT039545 53.00 WAT039555 53.00 WAT039565 53.00 Format Part Number Price NTSC PAL WAT054295 WAT054325 16.00 16.00 Sep-Pak® Cartridge Experiment Kit This activity is designed to demonstrate the principles of liquid chromatography using a grape drink mix and Sep-Pak® C18 Plus cartridges. This experiment lends itself ideally to a classroom setting. Description Cartridge Experiment Kit (Includes two Sep-Pak C18 cartridges and 1 pack of grape mix with instructions) Part No. Price WAT088253 15.00 ® © 2001 Waters Corporation 26 Sample Preparation Solid-Phase Extraction Sep-Pak® cartridges are available in a variety of designs; each offers specific functional benefits but all contain the same high-quality packing sorbents. This makes transfer of methods from one design to another straightforward and predictable. Sep-Pak® Plus Cartridges for Maximum Versatility Sep-Pak® Plus cartridges are designed for both convenient manual use and compatibility with automated instruments. They come in short body and long body configurations and are constructed from high purity polyethylene with female Luer inlets and male Luer outlets. They can be connected to many different types of devices such as positive flow pumps and syringes, vacuum manifolds, and automated sample processors. In many applications, the use of positive flow (pumps) provides for increased reproducibility in your results. Variations in recovery data, due to flow rate variability and the potential for air drying out a cartridge when using vacuum devices, can be eliminated. The male outlet tips feature reduced hold-up volume for precise fraction collection. The fittings also allow cartridges to be easily stacked for increased capacity or for the use of more than one sorbent type for cleanup of complex sample mixtures. Sep-Pak® Plus cartridges can be used with liquid or gas samples and have the unique capability for reverse flow direction, a useful feature for trace enrichment applications. The packing process and compression seal provide a uniform bed structure to minimize band spreading for the best possible separation efficiency. Bed volumes and weights of sorbent in the short and long body cartridges equal those of the corresponding Classic cartridges. All Sep-Pak® Plus cartridges have color-coded compression rings for general sorbent identification. Sep-Pak® Classic Cartridges Sep-Pak® Classic cartridges were the original SPE cartridges introduced over 20 years ago. Waters Sep-Pak® Classic cartridges contain the same amount of sorbent as the Sep-Pak® Plus cartridge. This means that you can convert existing methods easily to the more convenient Plus configuration. Sep-Pak® Light Cartridges for Limited Sample Volumes/Lower Solvent Consumption Sep-Pak® Light cartridges look similar to the short-body Sep-Pak® Plus cartridges, except for the distinctive “finned” outer body. The functional difference is the reduced internal diameter which results in bed volumes about one-third that of the corresponding Plus cartridge. This design allows you to elute fractions in a minimal volume to improve recoveries and reduce solvent consumption, a benefit for “sample-limited” applications or where excessive dilution is a concern. In addition, the Sep-Pak® Light cartridges are specially designed to provide efficient packed sorbent beds for use in downsizing current applications to reduce solvent consumption. All Sep-Pak® Light cartridges have color-coded compression rings for general sorbent identification. Sep-Pak® Vac RC Cartridges for Manual or Robotic Sample Preparation These cartridges feature a 20 cc “funnel” shape design containing 100 or 500 mg of sorbent. They can be used with robotic sample preparation equipment or with vacuum manifolds. The integral reservoir design allows for the convenient processing of larger amounts of sample or solvents. This reduces setup time and costs associated with the use of separate reservoirs. Color-coded printing on the cartridges makes sorbent identification easy. Economical Sep-Pak® Vac Cartridges Sep-Pak® Vac cartridges provide you with the option of even lower cost sample preparation. The cartridges use molded polypropylene “syringebarrel” bodies and two polyethylene filters to contain the packing material. A standard male Luer outlet tip permits use with most multiposition vacuum manifolds. Cartridge sizes range from 1 cc to 35 cc and sorbent weights range from 50 mg up to 10 grams. Sizes up to 6 cc can be used on many automated sample preparation systems designed to use “syringe-barrel” style cartridges. Sep-Pak® Vac cartridges offer the highest quality at low cost with a complete range of capacities for use in optimizing your methods. Color-coded printing on the syringe-barrel cartridges makes sorbent identification easy. Both the Sep-Pak® Plus and Classic designs have two different sizes (short body and long body) depending on the type of sorbent. Sep-Pak® C18 Environmental Cartridges This is a special “long body” Plus cartridge design containing approximately 1 gram of the monofunctional C18 or the trifunctional C18 sorbent. These cartridges are especially useful in trace enrichment applications. © 2001 Waters Corporation 27 Sample Preparation Which Cartridge Design is Best for Your Application Sample Preparation The Anatomy of Sep-Pak® Plus and Classic Cartridge Design The Anatomy of Sep-Pak® Vac and Vac RC Cartridge Designs Sep-Pak® Classic Sep-Pak® Plus Sep-Pak® Vac Sep-Pak® Vac RC 1 7 5 2 3 4 5 6 6* 1 7 2 3 11 10 4 8 8 9 6 9 1. Female Luer inlet accepts male Luer tip. Plus cartridge design can be stacked. 2. Highest quality sorbents designed and made specifically for sample preparation; clean, dry, reproducible in activity and capacity with optimal surface area, pore, and particle size distributions. 2. Broad range of sorbent surface activities available; each lot is tested under rigid specifications for chromatographic activity, retention and selectivity. Broad range of sorbent surface activities available; each lot is tested under rigid specifications for chromatographic activity, retention and selectivity. 3. Sorbent type and bed dimensions equal to corresponding Sep-Pak® Classic cartridges enable direct transfer of previously developed and published methods to new Plus design. Weight of sorbent in each cartridge is controlled within +/- 5% of specification to assure reproducible performance. 4. Special blend of HD and UHMW polyethylenes used for 20 micron frits. 5. Molded, medical grade polypropylene body. 5. Weight of sorbent in each cartridge is controlled within +/- 5% of specification to assure reproducible performance. 6. Cartridges are sealed in a special polyfoil pouch to protect product integrity, sorbent activity and purity. 6. Advanced bed formation technology to minimize voids and channels. 6* Patented Radial Compression Technology used to form homogeneous packed bed free of voids and channels. 7. Integral reservoir approximately 20 mL, robotic compatible. 8. Outlet male Luer tip. 9. Color-coded labeling on the cartridge to identify the sorbent. 3. 4. 7. Special blend of HD and UHMW polyethylenes used for 20 micron frits imparts excellent solvent resistance, extremely low extractables level, and good flow properties. Frit also acts as depth filter for small amounts of sample debris. 8. Polyethylene body has excellent solvent resistance. All body parts are quality tested to verify extremely low level of UV-absorbing extractables. Plus design is molded for precise dimensions making it suitable for automated equipment. 9. Cartridges are sealed in a special polyfoil pouch to protect product integrity, sorbent activity and purity. 1. Highest quality sorbents designed and made specially for sample preparation; clean, dry, reproducible in activity and capacity, with optimal surface area pore and particle size distributions. 10. Male Luer outlet has reduced internal volume for minimal sample hold-up. 11. Color-coded ring compresses and seals the cartridge and identifies sorbent. P.D. McDonald, C. W. Rausch, Radial Compression of Packed Beds, U.S. Patent # 4,250,035 (1981); Great Britain # 1,569,700 (1976); Canada # 1,101,785 (1981); Japan # 1,400,983 (1987); Sweden # 450,750 & # 453,437 (1987); Germany # 2,655,650 (1988); other patents pending. © 2001 Waters Corporation 28 Sample Preparation Which Sorbent is Best For Your Application The text and tables that follow give you detailed information on the properties of the sorbents that Waters offers. The Original C18 Sorbent Waters original C18 sorbent is the most widely referenced SPE product, with over 2,300 published papers or articles specifying its use. This product is made using the most reproducible bonding technology available, utilizing monofunctional silane chemistry. Waters original C18 sorbent offers a unique selectivity and excellent recovery of analytes, and is recommended for most applications where HPLC is utilized for subsequent analysis. tC18 (trifunctional) Sorbent With the addition of tC18 sorbent, you can now choose between two highly reproducible C18 chemistries, monofunctional and trifunctional. Trifunctional tC18 cartridges are recommended for most applications involving GC, GC/MS or LC/MS especially if long sample contact times are involved. This new tC18 sorbent has also become increasingly popular in pharmaceutical applications when a high carbon load, very hydrophobic surface is required. (See Table 1). Table 1: Recommendations for Using C18 and tC18 Sorbents Sample Contact Time long (> 10 minutes) short (< 10 minutes) Sample pH <3 3-7 >7 Detection Method HPLC Ultraviolet/Visible Absorbance Fluorescence GC Flame Ionization Mass Selective Electron Capture Nitrogen-Phosphorous Selectivity Unique Similar to other vendors’ C18 cartridges Key: R = recommended sorbent O = may also be applicable C18 tC18 NR R R O NR R NR R O R R R O O O O O O R R R R R NR NR R Sample Preparation Selecting the most appropriate sorbent is best approached from both a theoretical and empirical standpoint. Knowledge of the components in a specific sample will help you determine the general mode of separation, such as, normal phase, reversed-phase, or ion exchange, and more specific knowledge may help you select between the various types of sorbents in each mode. However, it is frequently necessary to determine the optimal separation by empirical means. NR = not recommended tC2 (trifunctional) Sorbent Many applications require a less hydrophobic (relative to C18), short carbon chain sorbent to provide optimal sample processing of a wide range of analytes. However, finding a reproducible, stable C2 sorbent can be difficult. Waters has recently developed a new, state-of-the-art trifunctional bonding process that provides you with the most dependable sorbents (tC2 and tC18) of this type available. The tC2 is ideal for many types of applications such as environmental and pharmaceutical. TM Visit Waters on-line product resource today © 2001 Waters Corporation 29 Sample Preparation Table 2: Sorbent Properties and Typical Applications Separation Mode Sorbent Properties & Applications Reversed-Phase C18 silica-based bonded phase with strong hydrophobicity; used to adsorb analytes of even weak hydrophobicity from aqueous solutions; typical applications include drugs and their metabolites in serum, plasma or urine, desalting of peptides, trace organics in environmental water samples, organic acids in beverages; similar in behavior to reversed-phase HPLC columns tC18 silica-based bonded phase with strong hydrophobicity; trifunctional bonding chemistry gives it an increased hydrolytic stability over C18; applications similar to C18 C8 silica-based bonded phase with moderate hydrophobicity; use for methods requiring less retention than C18; typical applications include drugs and their metabolites in serum, plasma or urine, peptides in serum, plasma tC2 silica-based bonded phase with low hydrophobic character; use for methods requiring less retention than C8; applications are similar to C18 and C8 Porapak RDX specially cleaned polymeric poly(divinylbenzene-vinylpyrrolidone) resin with hydrophobic character. Can be used as an alternative to octadecyl-bonded silica for preparation of analytes that weakly adsorb to silica-based reversed-phase sorbents. Compatible with sample or eluents at high and low pH. Specifically designed for the concentration of high explosives in aqueous samples. ™ Reversed or Normal Phase: Amino Propyl (NH2) silica-based polar bonded phase with basic character; can be used as a polar sorbent, like silica, with different selectivity for acidic/basic analytes or as weak anion exchanger in aqueous medium; applications include phenols and phenolic pigments, petroleum fractionation, saccharides and drugs and metabolites Cyano Propyl (CN) silica-based polar bonded phase; can be used as less polar alternative to silica in normal-phase applications or as less hydrophobic alternative to C18 or C8 in reversed-phase applications; typical applications include drugs, drug metabolites and pesticides Diol silica-based polar bonded phase with neutral character; can be used as an alternative to silica in normal phase applications, where the acidic character of silica is undesirable or as very weakly interacting phase in aqueous applications; applications include antibiotics from cosmetics; isolation of proteins or peptides by hydrophobic interaction chromatography Normal Phase Silica polar sorbent, used primarily to adsorb analytes from non polar solvents like hydrocarbons, chloro- or fluoro-substituted hydrocarbons or less polar esters and ethers; elution with more polar solvents like polar esters, ethers, alcohols, acetonitrile or water; the binding mechanism can be hydrogen bonding or dipole-dipole interaction; silica can also be used in aqueous medium as a cation exchanger of intermediate strength. Alumina (A, B & N) similar in use to silica; available in acidic, basic and neutral high activity grades; alumina also exhibits specific interactions with the pi-electrons of aromatic hydrocarbons, making it useful for applications like crude oil fractionation; acidic and basic grades can also be used as low-capacity ion-exchangers Florisil® polar, highly active, weakly basic sorbent for adsorption of low to moderate polarity species from nonaqueous solutions; specifically designed for the adsorption of pesticides using official AOAC and EPA methods; other applications include polychlorinated biphenyls (PCB’s) in transformer oil Ion-Exchange Accell™ Plus QMA silica-based hydrophilic strong anion-exchanger with large pore-size; extraction of anionic analytes in aqueous and non-aqueous solutions; due to the large pore-size, it is excellent for the isolation of anionic proteins, e.g., immunoglobulins, enzymes; other applications include the removal of acidic pigments from wines, fruit juices and food extracts, isolation of phenolic compounds, peptide pool fractionations Accell™ Plus CM silica-based hydrophilic weak cation-exchanger with large pore-size; extraction of cationic analytes in aqueous and non-aqueous solutions; due to the large pore-size, it is excellent for the isolation of cationic proteins; other applications include pesticides, herbicides, steroids Specialty DNPH-Silica, XPoSure™ contains acidified dinitrophenylhydrazine reagent coated on a silica sorbent. Used for the collection of air samples and subsequent quantitation of aldehydes and ketones by reaction to form the hydrazone derivative, and analysis by HPLC. DNPH-Silica is specified in several EPA procedures for the analysis of carbonyl compounds in air. © 2001 Waters Corporation 30 Sample Preparation Table 3: Sep-Pak® Cartridge Sorbents Physicochemical Characteristics Endcapped Percent Carbon Pore Size (nominal, Å) Particle Size Range (µm) C18 tC18 N/A N/A Yes Yes 12 17 125 125 55-105 37-55 C8 N/A Yes 9 125 37-55 tC2 N/A Yes 2.7 125 37-55 NH2 N/A No 3.5 125 55-105 CN N/A Yes 6.5 125 55-105 Diol N/A No 2 300 37-55 Silica High* N/A N/A 125 55-105 Florisil High** N/A N/A 60 50-200 Alumina A 1*** N/A N/A 120 50-300 Alumina N 1*** N/A N/A 120 50-300 Alumina B 1*** N/A N/A 120 50-300 Accell Plus CM N/A N/A 5.5 300 37-55 Accell Plus QMA N/A N/A 6 300 37-55 Porapak RDX N/A N/A 95 200 125-150 DNPH-Silica N/A N/A N/A 125 55-105 XPoSure N/A N/A N/A 125 500-1000 *** Moisture content ≤ 1.5%; activity ≤ 1 on Brockmann activity scale ® ™ ™ ® ™ * Moisture content ≤ 3.2% ** Moisture content ≤ 2.5% Sample Preparation Activity Grade Sorbent Table 4: Sep-Pak® Cartridges: Chemistry of Bonded-Phase Sorbents Sorbent Chemistry Bonding Chemistry Surface Functionality C18 Monofunctional silane –Si(CH3)2C18H37 tC18 Trifunctional silane –SiC18H37 C8 Monofunctional silane –Si(CH3)2C8H17 tC2 Trifunctional silane -SiC2H5 NH2 Trifunctional silane –Si(CH2)3NH2 CN Difunctional silane –Si(CH3)(CH2)3CN Diol Trifunctional silane –Si(CH2)3OCH2CH(OH)CH2OH Accell Plus CM Polymeric* –CO2- Na+ ™ Accell Plus QMA Polymeric** –C(O)NH(CH2)3N(CH3)3+ Cl- DNPH-Silica Coated*** ™ * An acrylic acid/acrylamide copolymer on Diol silica; ligand density: 350 µmoles/g; approximate protein binding capacity = 175 mg Cytochrome c/g of sorbent or 63 mg/Accell Plus CM Classic or Plus cartridge. ** An acrylamide copolymer on Diol silica; ligand density: 220 µmoles/g; approximate protein binding capacity = 200 mg BSA/g of sorbent or 72 mg/ Accell Plus QMA Classic or Plus cartridge. *** Unbonded silica sorbent coated with 14 µmoles DNPH/g of sorbent or 5 µmoles/cartridge. © 2001 Waters Corporation 31 Sample Preparation Table 5: Nominal Weight of Sorbent (in milligrams) Separation Mode and Sorbent Cartridge Type Classic Light General Surface Chemistry 360 130 Neutral N/A 145 Neutral N/A N/A 145 145 Neutral Neutral 360 360 360 360 360 360 130 130 130 Basic Neutral Neutral 690 910 1710 1710 1710 690 900 1850 1850 1850 120 145 280 280 280 Neutral Basic Acidic Basic Neutral 360 360 360 360 130 130 Basic Acidic Plus Reversed-phase 360 820 400 900 400 400 C18 Environmental C18* tC18 Environmental tC18* C8 tC2 Normal or Reversed-phase Amino Propyl (NH2)** Cyano Propyl (CN) Diol Normal Phase Silica Florisil® Alumina A Alumina B Alumina N Ion-Exchange ** Accell™ Plus QMA Accell™ Plus CM * ** Sep-Pak® Plus long-body cartridge. The amino propyl packing may also be used in the protonated form as a weak anion exchanger. Table 6: Sep-Pak® Vac Cartridges Nominal Weight of Sorbent Syringe Size 1 cc 50 mg 100 mg X X Vac RC 3 cc 200 mg 500 mg X X X 6 cc X 20 cc 2g 5g X X X 35 cc X See Cartridge Selection Guide for specific product availability. © 2001 Waters Corporation 32 10 g X Vac RC 12 cc 1g Sample Preparation Normal Phase Chromatography with Sep-Pak® Cartridges Sample Preparation To perform normal phase chromatography with Sep-Pak® cartridges, use a gradient of nonpolar solvents with polar Silica, Florisil®, NH2, Diol, CN, Alumina A, B, or N as a sorbent*. 1. You may condition the cartridge with six to ten hold-up volumes of nonpolar solvent, usually the sample solvent. 2. Load the sample into the cartridge. 3. Elute unwanted components with a nonpolar solvent. 4. Elute the first component of interest with a polar solvent. 5. Elute remaining components of interest with progressively more polar solvents. 6. When you recover all of your components, discard the used cartridge in an appropriate manner. * Depending upon your chromatographic conditions, you may also use CN as a packing material for normal phase chromatography. Reversed-Phase Chromatography with Sep-Pak® Cartridges To perform reversed-phased chromatography with Sep-Pak® cartridges, use a gradient of strongly to weakly polar solvents with nonpolar C18, tC18, C8, tC2, Diol, NH2 or CN as a sorbent. 1. Solvate the bonded phase with six to ten cartridge hold-up volumes of methanol or acetonitrile. Flush the cartridge with six to ten hold-up volumes of water or buffer. Do not allow the cartridge to dry out. 2. Load the sample dissolved in strongly polar solvent. 3. Elute unwanted components with a strongly polar solvent. 4. Elute weakly held components of interest with a less polar solvent. 5. Elute more tightly bound components with progressively more nonpolar solvents. 6. When you recover all of your components, discard the used cartridge in an appropriate manner. Ion-Exchange Chromatography with Sep-Pak® Cartridges To perform ion-exchange chromatography with Sep-Pak® cartridges, use a gradient of pH or ionic strength with Accell™ Plus CM, Accell Plus QMA or NH2 as a sorbent. 1. Condition the cartridge with six to ten hold-up volumes of deionized water or weak buffer. 2. Load the sample dissolved in a solution of deionized water or buffer. 3. Elute unwanted weakly bound components with a weak buffer. 4. Elute the first component of interest with a stronger buffer (change the pH or ionic strength). 5. Elute other components of interest with progressively stronger buffers. 6. When you recover all of your components, discard the used cartridge in an appropriate manner. © 2001 Waters Corporation 33 Sample Preparation Sep-Pak® Cartridge Selection Guide Vac 3 cc 200 mg Waters Sep-Pak® Vac Classic Light Vac 1 cc 50 mg Box of 50 Box of 50 Box of 50 C18 WAT020515 WAT051910 WAT023501 tC18 WAT036810 N/A C8 WAT036775 tC2 Vac 1 cc 100 mg Box of 100 Box of 100 Box of 50 Box of 50 WAT054955 WAT023590 WAT036935 WAT054945 WAT036805 WAT054960 WAT036820 WAT043410 WAT054925 N/A WAT036770 WAT054965 WAT036785 WAT043415 WAT054940 WAT052720 N/A WAT052725 N/A WAT052710 N/A N/A Silica WAT020520 WAT051900 WAT023537 WAT054980 WAT023595 WAT036940 WAT054930 Florisil® WAT020525 WAT051960 WAT023543 WAT054985 WAT023600 WAT043420 N/A Accell™ Plus CM WAT020550 WAT010910 WAT023531 N/A WAT023625 WAT043465 N/A Accell™ Plus QMA WAT020545 WAT010835 WAT023525 N/A WAT023620 WAT043460 N/A Alumina A WAT020500 WAT051800 WAT023549 N/A WAT023575 WAT043450 N/A Alumina B WAT020505 WAT051820 WAT023555 N/A WAT023580 WAT043455 N/A Alumina N WAT020510 WAT051810 WAT023561 N/A WAT023585 WAT043445 N/A Amino Propyl (NH2) WAT020535 WAT010830 WAT023513 N/A WAT023610 WAT043475 N/A Cyano Propyl (CN) WAT020540 WAT010823 WAT023507 WAT054975 WAT023615 WAT043470 WAT054935 Diol WAT020530 WAT010818 WAT023519 N/A WAT023605 WAT043480 N/A Waters Sep-Pak® Vac Vac RC 100 mg Waters Sep-Pak® Vac Plus Sorbent mg = nominal weight of sorbent in milligrams. See Sep-Pak® cartridge configuration and nominal weight of sorbent chart on page 32 for specific values of each sorbent. © 2001 Waters Corporation 34 Sample Preparation Vac RC 500 mg Vac 6 cc 500 mg Vac 6 cc 1 gram Vac 12 cc 2 gram Vac 20 cc 5 gram Vac 35 cc 10 gram Waters Sep-Pak® Vac Waters Sep-Pak® Vac Waters Sep-Pak® Vac Waters Sep-Pak® Vac Waters Sep-Pak® Vac Waters Sep-Pak® Vac Sample Preparation Vac 3 cc 500 mg Sorbent Box of 50 Box of 50 Box of 30 Box of 30 Box of 20 Box of 20 Box of 10 WAT020805 WAT036945 WAT043395 WAT036905 WAT036915 WAT036925 WAT043345 C18 WAT036815 WAT043425 WAT036790 WAT036795 WAT043380 WAT043365 WAT043350 tC18 WAT036780 WAT043430 WAT054525 WAT054570 WAT054615 WAT054660 WAT054700 C8 WAT052715 N/A N/A WAT052705 N/A N/A N/A tC2 WAT020810 WAT036950 WAT043400 WAT036910 WAT036920 WAT036930 WAT043355 Silica WAT020815 WAT043435 WAT043405 WAT043390 WAT043385 WAT043370 WAT043360 Florisil® WAT020855 WAT054505 WAT054545 WAT054590 WAT054635 WAT054675 WAT054720 Accell™ Plus CM WAT020850 WAT054500 WAT054550 WAT054595 WAT054640 WAT054680 WAT054725 Accell™ Plus QMA WAT020820 WAT043490 WAT054535 WAT054580 WAT054620 WAT054670 WAT054710 Alumina A WAT020825 WAT043495 WAT054540 WAT054585 WAT054625 WAT054665 WAT054715 Alumina B WAT020830 WAT043485 WAT054530 WAT054575 WAT054630 WAT043375 WAT054705 Alumina N WAT020840 WAT054515 WAT054560 WAT054605 WAT054650 WAT054695 WAT054740 Amino Propyl (NH2) WAT020835 WAT054510 WAT054555 WAT054600 WAT054645 WAT054685 WAT054730 Cyano Propyl (CN) WAT020845 WAT054520 WAT054565 WAT054610 WAT054655 WAT054690 WAT054735 Diol mg = nominal weight of sorbent in milligrams. See Sep-Pak® cartridge configuration and nominal weight of sorbent chart on page 32 for specific values of each sorbent. © 2001 Waters Corporation 35 Sample Preparation Sep-Pak® Plus Cartridges Box of 50 C18 tC18 (trifunctional) C8 tC2 (trifunctional) Silica Florisil® Accell™ Plus CM Accell™ Plus QMA Alumina A Alumina B Alumina N Amino Propyl (NH2) Cyano Propyl (CN) Diol Part No. Price WAT020515 WAT036810 WAT036775 WAT052720 WAT020520 WAT020525 WAT020550 WAT020545 WAT020500 WAT020505 WAT020510 WAT020535 WAT020540 WAT020530 127.00 127.00 127.00 127.00 127.00 127.00 132.00 132.00 127.00 127.00 127.00 127.00 127.00 132.00 Part No. Price WAT051910 WAT051900 WAT051960 WAT010910 WAT010835 WAT051800 WAT051820 WAT051810 WAT010830 WAT010823 WAT010818 134.00 134.00 134.00 140.00 140.00 134.00 134.00 134.00 134.00 134.00 140.00 Part No. Price WAT023501 WAT036805 WAT036770 WAT052725 WAT023537 WAT023543 WAT023531 WAT023525 WAT023549 WAT023555 WAT023561 WAT023513 WAT023507 WAT023519 114.00 114.00 114.00 114.00 114.00 114.00 120.00 120.00 114.00 114.00 114.00 114.00 114.00 120.00 Sep-Pak® Plus cartridges come in short body (C18, tC18, C8, tC2, Accell Plus QMA and CM, NH2, CN, Diol) and long body (Silica, Florisil, Alumina A,B,N) configurations. They are stackable, allowing for increased capacity or for the use of more than one sorbent. Sep-Pak® Plus cartridges are compatible with some automated sample processors. Sep-Pak® Classic Cartridges Box of 50 C18 Silica Florisil® Accell™ Plus CM Accell™ Plus QMA Alumina A Alumina B Alumina N Amino Propyl (NH2) Cyano Propyl (CN) Diol Sep-Pak® Classic cartridges also come in short body (C18, Accell Plus QMA and CM, NH2, CN, Diol) and long body (Silica, Florisil, Alumina A,B,N) configurations. Waters original Sep-Pak® cartridges, radially compressed “mini-columns”, for versatile easy column liquid-solid extraction. Sep-Pak® Light Cartridges Box of 50 C18 tC18 (trifunctional) C8 tC2 (trifunctional) Silica Florisil® Accell™ Plus CM Accell™ Plus QMA Alumina A Alumina B Alumina N Amino Propyl (NH2) Cyano Propyl (CN) Diol © 2001 Waters Corporation 36 For “sample limited” applications or where excessive dilution is a concern, Sep-Pak® Light cartridges contain less sorbent than the standard Sep-Pak® cartridge and can give higher sample recovery with less solvent consumption for limited sample situations. Sample Preparation Sep-Pak® Vac SPE Cartridges Sample Preparation Waters Sep-Pak® Vac solid-phase extraction columns come in a wide range of syringe-barrel configurations, from 1 cc to 35 cc, for all your high volume batch processing and automated sample prep needs. They are packed with a variety of sorbent weights from 50 mg to 10 grams to handle all your sample load requirements. Sep-Pak® Vac cartridges provide the method reproducibility you need for dependable analytical results and they are registered with the FDA as Class 1 medical Devices. They are manufactured in strict compliance with cGMP guidelines in manufacturing facilities certified to meet the ISO 9002 standard. Sep-Pak® Vac 1 cc Cartridges (contains 50 mg of sorbent) Box of 100 C18 tC18 (trifunctional) C8 Silica Florisil® Cyano Propyl (CN) Part No. Price WAT054955 WAT054960 WAT054965 WAT054980 WAT054985 WAT054975 107.00 107.00 107.00 95.00 107.00 151.00 For information on the availability of other sorbents, please contact your local sales representative. Sep-Pak® Vac 1 cc Cartridges (contains 100 mg of sorbent) Box of 100 C18 tC18 (trifunctional) C8 tC2 (trifunctional) Silica Florisil® Accell™ Plus CM Accell™ Plus QMA Alumina A Alumina B Alumina N Amino Propyl (NH2) Cyano Propyl (CN) Diol Part No. Price WAT023590 WAT036820 WAT036785 WAT052710 WAT023595 WAT023600 WAT023625 WAT023620 WAT023575 WAT023580 WAT023585 WAT023610 WAT023615 WAT023605 115.00 115.00 115.00 115.00 105.00 115.00 169.00 169.00 115.00 115.00 115.00 169.00 169.00 169.00 Sep-Pak® Vac 3 cc Cartridges (contains 200 mg of sorbent) Box of 50 C18 tC18 (trifunctional) C8 Silica Cyano Propyl (CN) Part No. Price WAT054945 WAT054925 WAT054940 WAT054930 WAT054935 83.00 82.00 83.00 78.00 102.00 Sep-Pak® Vac 3 cc Cartridges (contains 500 mg of sorbent) Box of 50 C18 tC18 (trifunctional) C8 tC2 (trifunctional) Silica Florisil® Accell™ Plus CM Accell™ Plus QMA Alumina A Alumina B Alumina N Amino Propyl (NH2) Cyano Propyl (CN) Diol Part No. Price WAT020805 WAT036815 WAT036780 WAT052715 WAT020810 WAT020815 WAT020855 WAT020850 WAT020820 WAT020825 WAT020830 WAT020840 WAT020835 WAT020845 91.00 91.00 91.00 91.00 87.00 91.00 110.00 110.00 91.00 91.00 91.00 110.00 110.00 110.00 Sep-Pak® Vac 6 cc Cartridges (contains 500 mg of sorbent) Box of 30 C18 tC18 (trifunctional) C8 Florisil® Accell™ Plus CM Accell™ Plus QMA Alumina A Alumina B Alumina N Amino Propyl (NH2) Cyano Propyl (CN) Diol Part No. Price WAT043395 WAT036790 WAT054525 WAT043405 WAT054545 WAT054550 WAT054535 WAT054540 WAT054530 WAT054560 WAT054555 WAT054565 76.00 76.00 76.00 69.00 92.00 92.00 69.00 69.00 69.00 92.00 92.00 92.00 © 2001 Waters Corporation 37 Sample Preparation Sep-Pak® Vac 6 cc Cartridges (contains 1 g of sorbent) Box of 30 C18 tC18 (trifunctional) C8 tC2 (trifunctional) Silica Florisil® Accell™ Plus CM Accell™ Plus QMA Alumina A Alumina B Alumina N Amino Propyl (NH2) Cyano Propyl (CN) Diol Sep-Pak® Vac 35 cc Cartridges (contains 10 g of sorbent) Part No. Price WAT036905 WAT036795 WAT054570 WAT052705 WAT036910 WAT043390 WAT054590 WAT054595 WAT054580 WAT054585 WAT054575 WAT054605 WAT054600 WAT054610 92.00 92.00 92.00 92.00 78.00 78.00 139.00 139.00 78.00 78.00 78.00 139.00 139.00 139.00 Sep-Pak® Vac 12 cc Cartridges (contains 2 g of sorbent) Box of 20 C18 tC18 (trifunctional) C8 Silica Florisil® Accell™ Plus CM Accell™ Plus QMA Alumina A Alumina B Alumina N Amino Propyl (NH2) Cyano Propyl (CN) Diol Box of 10 C18 tC18 (trifunctional) C8 Silica Florisil® Accell™ Plus CM Accell™ Plus QMA Alumina A Alumina B Alumina N Amino Propyl (NH2) Cyano Propyl (CN) Diol C18 tC18 (trifunctional) C8 Silica Florisil® Accell™ Plus CM Accell™ Plus QMA Alumina A Alumina B Alumina N Amino Propyl (NH2) Cyano Propyl (CN) Diol Part No. Price WAT043345 WAT043350 WAT054700 WAT043355 WAT043360 WAT054720 WAT054725 WAT054710 WAT054715 WAT054705 WAT054740 WAT054730 WAT054735 131.00 133.00 133.00 78.00 78.00 185.00 185.00 78.00 78.00 78.00 185.00 185.00 185.00 WAT036915 WAT043380 WAT054615 WAT036920 WAT043385 WAT054635 WAT054640 WAT054620 WAT054625 WAT054630 WAT054650 WAT054645 WAT054655 100.00 100.00 100.00 69.00 69.00 139.00 139.00 69.00 69.00 69.00 139.00 139.00 139.00 Description Qty Part No. Price 30 cc Reservoir 60 cc Reservoir Reservoir Adapters for 1, 3 and 6 cc VAC Reservoir Adapters for 12, 20 and 35 cc VAC Male-Male Adapter Male Luer Plugs Female Luer Plugs 48 12 WAT011390 WAT024659 49.00 12.00 12 WAT054260 35.00 10 100 WAT048160 WAT024310 WAT044395 WAT044385 165.00 55.00 32.00 32.00 Part No. Price WAT036925 WAT043365 WAT054660 WAT036930 WAT043370 WAT054675 WAT054680 WAT054670 WAT054665 WAT043375 WAT054695 WAT054685 WAT054690 163.00 163.00 163.00 100.00 100.00 210.00 210.00 100.00 100.00 100.00 210.00 210.00 210.00 © 2001 Waters Corporation 38 Price Sep-Pak® Cartridge Accessories Sep-Pak® Vac 20 cc Cartridges (contains 5 g of sorbent) Box of 20 Part No. Sample Preparation Sep-Pak® Vac RC robotic compatible extraction cartridges contain the same quality and amount of sorbent as our 1 cc - 100 mg and 3 cc - 500 mg Sep-Pak® Vac cartridges which makes methods transfer easier. Sep-Pak® Vac RC Cartridges (contains 100 mg of sorbent) Box of 50 C18 tC18 (trifunctional) C8 Silica Florisil® Accell™ Plus CM Accell™ Plus QMA Alumina A Alumina B Alumina N Amino Propyl (NH2) Cyano Propyl (CN) Diol Part No. Price WAT036935 WAT043410 WAT043415 WAT036940 WAT043420 WAT043465 WAT043460 WAT043450 WAT043455 WAT043445 WAT043475 WAT043470 WAT043480 78.00 78.00 78.00 69.00 69.00 105.00 105.00 69.00 69.00 69.00 105.00 105.00 105.00 Sep-Pak® Vac RC Cartridges (contains 500 mg of sorbent) Box of 50 C18 tC18 (trifunctional) C8 Silica Florisil® Accell™ Plus CM Accell™ Plus QMA Alumina A Alumina B Alumina N Amino Propyl (NH2) Cyano Propyl (CN) Diol TM Part No. Price WAT036945 WAT043425 WAT043430 WAT036950 WAT043435 WAT054505 WAT054500 WAT043490 WAT043495 WAT043485 WAT054515 WAT054510 WAT054520 110.00 110.00 110.00 92.00 92.00 146.00 146.00 92.00 92.00 92.00 146.00 146.00 146.00 Visit Waters on-line product resource today © 2001 Waters Corporation 39 Sample Preparation Sep-Pak® Vac RC Cartridges are labeled with sorbent type and weight and colorcoded for easy identification. Each cartridge contains either 500 mg or 100 mg of high quality sorbent and can be utilized by many robotic sample processors. Use Sep-Pak® Vac RC cartridges manually or with automated or robotic equipment. The “robotic compatible” extraction cartridges feature a 20 mL overall capacity which allows for larger conditioning /elution volumes. They eliminate the need for adapting reservoirs to standard size Vac cartridges making sample preparation more convenient. Sample Preparation The Waters Extraction Manifold for Use With Solid-Phase Extraction Cartridges. We are pleased to introduce a new vacuum manifold from Waters. The new extraction manifold incorporates improvements designed to make your solid phase extraction process easier, faster and more accurate. The vacuum manifold has the capacity to process up to twenty samples simultaneously. The extraction manifold has enhanced features designed for use with conventional silica-based solid phase extraction cartridges as well as modifications that allow you take full advantage of the unique performance characteristics of our Oasis® HLB extraction cartridges. B C A Just look at what this new manifold has to offer: A Precision machined Delrin® cover with alignment posts for quick and easy alignment with test tube rack. B Vacuum gauge placement on cover, not in fluid path allows for quick and easy waste removal at bottom by vacuum. C Enhanced vacuum control valve designed for use with Waters Oasis® HLB extraction cartridges, allows for a quick and momentary rise in vacuum above the frit bubble point at the touch of a finger. D High purity polypropylene needle valves and needle tips with minimum dead volume. D: Required when using silica-based SPE cartridges. Extraction Manifolds Description 20 position extraction manifold without rack (includes 20 needle tips, 25 plugs and ejector tool) 20 position extraction manifold with 13 mm x 75 mm test tube rack (includes 20 needle tips, 25 plugs and ejector tool) 20 position extraction manifold with 13 mm x 100 mm test tube rack (includes 20 needle tips, 25 plugs and ejector tool) 20 position extraction manifold with 16 mm x 75 mm test tube rack (includes 20 needle tips, 25 plugs and ejector tool) 20 position extraction manifold with 16 mm x 100 mm test tube rack (includes 20 needle tips, 25 plugs and ejector tool) © 2001 Waters Corporation 40 Part number Price WAT200677 540.00 WAT200606 635.00 WAT200607 635.00 WAT200608 635.00 WAT200609 635.00 Sample Preparation Accessories and Spare Parts for the Waters Extraction Manifolds Description pkg of 48 pkg of 12 pkg of 100 pkg of 12 pkg of 10 Price WAT200685 WAT200691 WAT200686 WAT200687 WAT200688 WAT200689 WAT200690 WAT058839 WAT058851 WAT058840 WAT200678 WAT200679 WAT200680 WAT200681 WAT011390 WAT024659 WAT024310 WAT054260 WAT048160 WAT058871 WAT085114 WAT085115 45.00 10.00 210.00 140.00 190.00 99.00 5.00 8.00 8.00 6.00 95.00 95.00 95.00 95.00 49.00 12.00 55.00 35.00 165.00 120.00 705.00 595.00 Sample Preparation Needle valves, pack of 20 (required when using silica-based SPE cartridges) (not required for use with Oasis® HLB Extraction Cartridges) Needle tips, pack of 20 Cover, 20 position without gauge assembly Gauge assembly, Vacuum Reservoir, glass with outlet valve Outlet valve kit Gasket for cover Ejector tool Luer Plugs, pack of 25 Rubber ball ring (for vacuum gauge assembly) 13 mm x 75 mm test tube rack 13 mm x 100 mm test tube rack 16 mm x 75 mm test tube rack 16 mm x 100 mm test tube rack Reservoir, 30 cc (for Sep-Pak® Plus, Light, Vac & Classic cartridges) Reservoir, 60 cc (for Sep-Pak® Plus, Light & Vac cartridges) Adapter, male-male Luer (for Sep-Pak® Classic cartridges) Adapter (to attach reservoir to 1, 3 & 6 cc Sep-Pak® Vac cartridges) Adapter (to attach reservoir to 12, 20 & 35 cc Sep-Pak® Vac cartridges) Reversible Vial Rack for 1 mL or 4 mL autosampler vials Vacuum pump (110V, 60 Hz) Vacuum pump (220V, 50 Hz) Part No. Accessories and Spare Parts for the Waters Vacuum Manifolds (Older Design with Blue Lid) 12, 16 & 24 Position Description C-Clips Cover, Blue Nylon, with Gasket and 12 Stopcocks Cover, Blue Nylon, with Gasket and 16 Stopcocks Cover, Blue Nylon, with Gasket and 24 Stopcocks Eluent Delivery Needles, Polypropylene Eluent Delivery Needles, Stainless Steel Gaskets Gaskets Gauge and Bleed Valve Assembly Luer Fitting, Female (Top of Cover, for Stopcocks) Luer Fitting, Male (Bottom of Cover, for Needles) Plastic Legs, Black Stopcocks Vacuum Chamber, Glass Vacuum Chamber, Glass Collection Rack Assembly Holder Plate, 10 mm Test Tubes or Autosampler Vials Holder Plate, 12 mm Test Tubes Holder Plate, 16 mm Test Tubes Holder Plate, Volumetric Flasks Collection Rack Assembly Holder Plate, 12 mm Test Tubes Holder Plate, 16 mm Test Tubes Collection Rack Assembly Holder Plate, 12 mm Test Tubes Holder Plate, 16 mm Test Tubes Reservoirs (for Sep-Pak Plus, Light, Classic Cartridges) Adapters (male-male) for Classic cartridges Position Size Quantity Part No. Price 12/16/24 12 16 24 12/16/24 12/16/24 12 16/24 12/16/24 12/16/24 12/16/24 12/16/24 12/16/24 12 16/24 12 12 12 12 12 16 16 16 24 24 24 12/16/24 12/16/24 12 1 1 1 12 12 2 2 1 2 2 4 12 1 1 1 1 1 1 1 1 1 1 1 1 1 48 100 WAT054822 WAT054800 WAT054801 WAT054802 WAT054808 WAT054807 WAT054803 WAT054804 WAT054799 WAT054809 WAT054810 WAT054805 WAT054806 WAT054797 WAT054798 WAT054811 WAT054812 WAT054813 WAT054814 WAT054815 WAT054816 WAT054817 WAT054818 WAT054819 WAT054820 WAT054821 WAT011390 WAT024310 20.00 150.00 235.00 265.00 12.00 39.00 8.00 14.00 95.00 4.00 4.00 10.00 15.00 85.00 115.00 85.00 35.00 35.00 35.00 35.00 105.00 50.00 50.00 105.00 50.00 50.00 49.00 55.00 © 2001 Waters Corporation 41 Sample Preparation Waters Alliance® Filtration System The introduction of the Alliance® Filtration System dramatically changes the way sample filtration is performed for Alliance® HPLC systems. In only seconds up to 24 samples can be simultaneously fitted into vials held in a Waters 2690 Separation module’s sample carousel. The Alliance® Filtration System eliminates the time-consuming alternative one-at-a-time syringe filter process. The Alliance® Filtration System is comprised of a manifold which houses the Alliance® sample carousel and a Pall Gelman Laboratory AcroPrep™ disposable filter plate containing 24 membrane filters. Here’s how it works: Simply place your Alliance® carousel with empty sample vials into the manifold. The manifold contains an alignment key which locks the carousel into place ensuring that sample vials in the carousel line up directly under the appropriate filter well on the AcroPrep™ filter plate. The AcroPrep™ filter plate is placed onto the manifold. Alignment posts on the manifold ensure that the filter wells line up directly over the appropriate sample vial in the carousel. Pipette your samples into the filter wells on the AcroPrep™ filter plate, apply a vacuum, and in seconds, the samples are drawn through the AcroPrep™ filter plate into the appropriate sample vials housed in the carousel. Once the samples are filtered, simply shut off the vacuum source, remove and dispose of the AcroPrep™ filter plate, cap your sample vials and you’re ready to run. For applications that require a filter prewash step, a waste tray is included with the Alliance® Filtration System. AcroPrep™ filter plates for the Alliance® Filtration System are available with 0.45 µm PTFE, PVDF, Nylon, and GH Polypro (GHP) membrane filters from Pall Gelman Laboratory. Some advantages the Alliance® Filtration System has over the conventional syringe filter technique Ease-of-Use: No need to use cumbersome, individually wrapped disposable syringes. With the Alliance® Filtration System you can use disposable pipette tips which are less costly, easier to use and require less bench space. Saves Time: The conventional syringe filter technique is a one-at-a-time filtration process. With the Alliance® Filtration System, you can simultaneously filter up to 24 samples, in only seconds, saving both time and labor. Saves Space: Using the conventional syringe filter technique requires significant bench space. The Alliance® Filtration System is compact and requires much less bench space. Affordable: When factoring in material and labor costs, the Alliance® Filtration System is comparable in cost to the conventional syringe filter technique. Improves Productivity: The conventional syringe filter technique requires 25 minutes of labor per carousel. The Alliance® Filtration System improves productivity in your lab by saving 20 minutes in labor time per carousel. The materials of construction used in the AcroPrep™ filter plates are the same as those used in the Pall Gelman Laboratory Acrodisc® syringe filters. If you are currently using Acrodisc® syringe filters for your sample filtration, this should eliminate the need to validate the AcroPrep™ filter plates. Material and labor requirements Syringe Filter Technique Alliance® Filtration System 24 disposable syringes 24 disposable pipette tips 24 syringe filters 1 AcroPrep™ filter plate 25 minutes 5 minutes © 2001 Waters Corporation 42 Sample Preparation Compatibility Chart for AcroPrep™ Filter Plates Alliance Filtration Manifold (complete) ® Solvent GHP Nylon PVDF PTFE Acetone Acetonitrile Acetic acid, glacial n-Butanol Chloroform Dioxane Dimethyl formamide Dimethyl sulfoxide Ethanol Ethyl acetate Ethyl ether Freon TF Solvent Hydrochloric acid (1N) Hexane, dry Methanol Methylene chloride Methyl ethyl ketone N-Methylpyrrolidone Isopropanol Sodium hydroxide (3N) Tetrahydrofuran Tetrahydrofuranl/water (50/50) Toluene Water R R R R R R R R R R R R GHP R R R R R R R R R R R R R R NR R R * R R R R R R Nylon NR R R R NR R R LR R R R R NR R R R R R NR NR R R R R PVDF R R R R NR NR R NR NR R R R R R R R R R R R R R R R PTFE R R R R R R R R R R R R Quantity Part No. Price 1 289000159 750.00 1 3/pkg 3/pkg 3/pkg 1 1 1 5 carousels 700000231 700000232 700000233 700000234 700000235 WAT085114 WAT085115 WAT270328 75.00 15.00 6.00 22.00 25.00 705.00 595.00 205.00 186000154 186000155 186000156 186000157 460.00 460.00 460.00 460.00 Sample Preparation Description Spare Parts and Accessories R = Resistant LR = Limited Resistance NR = Not Resistant * = Insufficient Data Glass Housing for Filtration Manifold O-Ring Kit Rubber Feet Manifold Alignment Post Waste Tray Vacuum Pump 110 V, 60Hz Vacuum Pump 220V, 50Hz Alliance® Carousel Kit AcroPrep™ Filter Plates for the Alliance® Filtration System AcroPrep™ GHP Filter Plates, 0.45 µm AcroPrep™ Nylon Filter Plates, 0.45 µm AcroPrep™ PTFE Filter Plates, 0.45 µm AcroPrep™ PVDF Filter Plates, 0.45 µm 10/pkg 10/pkg 10/pkg 10/pkg 2 mL (12 x 32 mm) Crimp Top and Snap Cap Vials and Accessories (6.0 mm neck opening) Recommended for use with the Alliance® Filtration System 12 x 32 mm clear glass vial, crimp top 12 x 32 mm amber glass vial, crimp top Polyurethane cap (0.44 mm with star) 12 x 32 mm clear glass vial, snap cap design, glass is Type 1, Class A 12 x 32 mm amber glass vial, snap cap design, glass is Type 1, Class B One-step plastic cap and septa (PTFE) Alliance® total recovery vial 100/pkg 100/pkg 100/pkg 100/pkg WAT094222 WAT094223 WAT094224 WAT094219 16.00 18.70 26.00 15.00 100/pkg WAT094220 17.50 100/pkg 100/pkg 186000328 186000234 18.50 98.00 Test Methods: The data presented in this chart is a compilation of testing by Pall Gelman Laboratory with certain chemicals, manufacturer’s data, or compatibility recommendations from the Compass Corrosion Guide, by Kenneth M. Pruett. This data is intended to provide expected results when filtration devices are exposed to chemicals under static conditions for 48 hours at 25 °C, unless otherwise noted. This chart is intended only as a guide. Accuracy cannot be guaranteed. Users should verify chemical compatibility with a specific filter under under actual use conditions. Chemical compatibility with a specific filter, under actual use conditions, is affected by many variables, including temperature, pressure, concentration, purity, and various chemical combinations which present complete accuracy. © 2001 Waters Corporation 43 Sample Preparation Pall Gelman Laboratory Sample and Solvent Filtration Products from Waters Membrane Choices Membrane Type (PTFE, GHP, PVDF, Nylon) — Typically, the first step in selecting a suitable filter is to choose a membrane that is chemically compatible with the sample (see chart opposite). For aqueous samples, it is preferable to use a hydrophilic membrane (GHP, PVDF or Nylon) versus a hydrophobic membrane (PTFE). With proteinaceous samples, a low protein binding filter (GHP or PVDF) is preferable to other hydrophilic filters (Nylon). GHP Acrodiscs Filtration of HPLC samples and solvents is a preventative maintenance procedure that saves your chromatography lab time and money. Filtration provides immediate protection for the components of your HPLC instrumentation by prolonging column life, minimizing downtime, and improving both the precision and accuracy of your data. Filtering samples and mobile phase prior to use decreases downtime by eliminating sample/solvent particulates that may contribute to: • erratic pressure fluctuations • noisy base lines • damage to pump pistons and seals, injector valves and plugged injector needles • noise in the detector • decreased column lifetime • HPLC certified • Hydrophilic polypropylene membrane offers excellent chemical compatibility and minimizes extractables • Suitable for aqueous and organic solvents • Low protein binding Nylon Acrodiscs • HPLC certified • Save time, no pre-wetting • Hydrophilic nylon membrane offers broad chemical compatibility Acrodisc® LC (PVDF) • HPLC certified • Hydrophilic PVDF membrane • Filters both aqueous and organic solvent solutions Pall Gelman Laboratory filters have been Certified for Compliance. That means they’ve been designed and developed to assist customers in complying with their regulatory and quality objectives. We have more membrane types such as hydrophilic polypropylene for broad chemical compatibility and low protein binding. Acrodiscs come in a variety of sizes, including the new minispike which is designed for filtering small volumes with minimum hold-up volume into narrow openings such as autosampler vials. The Acrodisc® membrane is housed in high purity polypropylene, resulting in exceptionally low extractable levels. Acrodisc® CR (PTFE) • HPLC certified • Excellent chemical compatibility • Used for aggressive and organic solvents Ion Chromatography (IC) Acrodiscs • IC certified to ensure low ionic backgrounds • Recommended for aqueous samples only GHP Acrodisc® GF and Nylon Acrodisc® GF • • • • Filter Design Choices Pall Gelman Laboratory’s broad range of device sizes assures complete sample filtration with minimal sample loss. In addition, many Acrodisc® syringe filters offer the option of a minispike outlet, rather than the traditional male luer outlet. The minispike outlet, available on 13 mm PTFE, Nylon, GH Polypro and PVDF Acrodisc® syringe filters, allows for easy dispensing into autosampler vials. Designed with a glass fiber prefilter over membrane Maximum throughputs for hard-to-filter samples HPLC certified Binder-free borosilicate glass fiber prefilter Glass Fiber Acrodiscs • 100% binder-free borosilicate glass fiber filter • Can be used alone or in series with another Acrodisc® • deal for prefiltration of 5-100 milliliters of serum Supor® (PES) • Hydrophilic polyethersulfone • For biological, pharmaceutical or sterilizing sample • Can be gamma sterilized or autoclaved 4mm PP Male Luer 13mm PP Male Luer 13mm PP Minispike 25mm PP Male Luer PP = Polypropylene housing Pore Size (0.2 and 0.45 µm) — The filter pore size is usually a personal preference of the chromatographer, but relates to the column packing material. Since there are many different packing materials, a general guideline is that most customers find that the 0.45 µm filter meets their requirements, although chromatographers are increasingly moving toward using 0.2 µm filters. © 2001 Waters Corporation 44 Sample Preparation Water Toluene Tetrahydrofuran/water (50/50) Tetrahydrofuran Sodium hydroxide (5N) Isopropanol N-Methylpyrrolidone Methyl ethyl ketone Methylene chloride Methanol Hexane, dry Hydrochloric acid (1N) Freon TF Ethyl ether Ethyl acetate Ethanol Dimethyl sulfoxide Dimethyl formamide Dioxane Chloroform n-Butanol Acetic acid, glacial Acetoniltrile = RESISTANT = LIMITED RESISTANCE = NOT RESISTANT = INSUFFICIENT DATA GH Polypro Syringe Filters GHP Acrodisc® 13 (13 mm) R R R R R R R R R R R R R R R R R R R R R R R R GHP Acrodisc® (25 mm) R R R R R R R R R R R R R R R R R R R R R R R R GHP Acrodisc® GF (25 mm) R R R R R R R R R R R R R R R R R R R R R R R R PTFE Syringe Filters Acrodisc® 4CR PTFE (4 mm) R* R R R LR R R* R* R R* R R R R R LR R* R* R LR LR • LR* R Acrodisc® 13CR PTFE (13 mm) R* R R R R R R* R* R R* R R R R R R R* R* R R R R R* R Acrodisc® CR PTFE (25 mm) R* R R R R R R* R* R R* R R R R R R R* R* R R R R R* R Acrodisc® LC13 PVDF (13 mm) NR* R R R R R NR* NR* R R* R R R R R R NR* NR* R NR R R R* R Acrodisc LC PVDF (25 mm) NR* R R R R R NR* NR* R R* R R R R R R NR* NR* R NR R R R* R Nylon Acrodisc® 4 (4 mm) R* R R R NR • R* R* R R* NR R NR R R NR R* R* R R NR LR R* R Nylon Acrodisc® 13 (13 mm) R* R R R NR • R* R* R R* NR R NR R R NR R* R* R R NR LR R* R Nylon Acrodisc® (25 mm) R* R R R NR • R* R* R R* NR R NR R R NR R* R* R R NR LR R* R Nylon Acrodisc GF (25 mm) R* R R R NR • R* R* R R* NR R NR R R NR R* R* R R NR LR R* R PVDF Syringe Filters ® Nylon Syringe Filters ® Ion Chromatography Syringe Filters IC Acrodisc® (13 mm & 25 mm) NR LR NR R NR • NR NR • LR R LR • LR R NR • NR • • NR • R R R R R R R R R R R R R R R R R R R R LR R R R R NR NR NR R NR NR R LR NR R NR NR NR R R NR NR NR R R Glass Fibre Syringe Filters GF Acrodisc® R Acrylic Copolymer Syringe Filters Non-sterile Acrodisc® (25 mm) NR NR NR R NR Disc Filters GH Polypro R R R R R R R R R R R R R R R R R R R R R R R NR R R R R LR NR NR R R R R R R R R LR NR R NR LR • R R Nylaflo (Nylon) R R NR R NR R R R R R R LR NR • LR NR NR R R R R R NR R TF (PTFE) R R R R R R R R R R R R R R R R R R R R R R R R FP Verciel (PVDF) Note: R = Resistant LR = Limited Resistance NR = Not Resistant No significant change was observed in flow rate or bubble point of the membrane. Moderate changes in physical properties or dimension of the membrane were observed. The filter may be suitable for short term, non-critical use at room temperature. The membrane is basically unstable. In most cases, extensive shrinkage or swelling occurs. The filter may gradually weaken or partially dissolve after extended exposure. * UV absorbance was set at 254 nm © 2001 Waters Corporation 45 Sample Preparation R LR NR • Acetone Filter Design and Membrane Choices Sample Preparation Part Number Cross Reference Pall Gelman Laboratory Description Whatman Part No. Millipore® Part No. Waters Part No. PTFE, 4 mm, 0.45 µm PTFE, 13 mm, 0.2 µm PTFE, 13 mm, 0.45 µm PTFE, 25 mm, 0.2 µm PTFE, 25 mm, 0.45 µm PVDF, 13 mm, 0.45 µm PVDF, 25 mm, 0.45 µm GHP, 13 mm, 0.45 µm GHP, 25 mm, 0.45 µm Nylon, 4 mm, 0.45 µm Nylon, 13 mm, 0.2 µm Nylon, 13 mm, 0.45µm Nylon, 25 mm, 0.2 µm Nylon, 25 mm, 0.45 µm Supor®, 25 mm, 0.45 µm Supor®, 25 mm, 0.2 µm PVDF, 47 mm, 0.45 µm PVDF, 47 mm, 0.2 µm Nylon, 47 mm, 0.45 µm Nylon, 47 mm, 0.2 µm PTFE, 47 mm, 0.45 µm PTFE, 47 mm, 0.2 µm GHP, 47 mm, 0.45 µm Supor®, 47 mm, 0.2 µm Supor®, 47 mm, 0.45 µm Supor®, 13 mm, 0.45 µm Glass Filter Holder Forceps 300 mL Funnel Glass Base, Tabulated Cap Ground Joint Flask Thick Glass Prefilter AVE Glass Prefilter 6783-0404 6783-1302 6783-1304 6784-2502 6784-2504 6792-1304 — 6784-1304 6785-2504 6790-0404 6790-1302 6790-1304 — — 6780-2504 6780-2502 — — 7404-004 7402-004 7585-004 7582-004 7002-0447 — — — — — — — — — — SJFHL04NS SJFG013NS SJFH013NS SLFG025NS SLSR025NS SJHV013NS SLHV025NS SJFH013NS SLCR025NS SJHV004NS — — — — SLHA025OS SLGS025OS HVLP04700 — — — FHLP04700 FGLP04700 FHLC04700 GSTF04700 HATF04700 HATF01300 XX1504700 XX6200006 XX1004704 XX1504702 XX1504705 AP2501000 AP4001000 WAT200508 WAT200506 WAT200502 WAT200504 WAT200500 WAT200512 WAT200510 WAT200516 WAT200514 WAT200526 WAT200524 WAT200520 WAT200522 WAT200518 WAT200528 WAT200529 WAT200530 WAT200531 WAT200532 WAT200533 WAT200534 WAT200535 WAT200537 WAT200539 WAT200538 WAT200540 WAT200543 WAT200544 WAT200545 WAT200546 WAT200547 WAT200541 WAT200542 © 2001 Waters Corporation 46 Sample Preparation Syringe Filters with PTFE Membrane Quantity Diameter Pore Size Acrodisc® CR pkg/50 case/200 pkg/100 case/300 pkg/250 case/750 pkg/100 case/300 pkg/50 case/200 pkg/100 case/300 pkg/100 case/300 25 mm 25 mm 13 mm 13 mm 4 mm 4 mm 13 mm 13 mm 25 mm 25 mm 13 mm 13 mm 13 mm 13 mm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.2 µm 0.2 µm 0.2 µm 0.2 µm 0.2 µm 0.2 µm Acrodisc® CR Minispike Acrodisc® CR Acrodisc® CR Minispike Part No. Price WAT200500 WAT200501 WAT200502 WAT200503 WAT200508 WAT200509 WAT200558 WAT200559 WAT200504 WAT200505 WAT200506 WAT200507 WAT200556 WAT200557 120.00 398.00 158.00 408.52 271.00 765.06 166.00 419.64 120.00 398.00 158.00 408.52 166.00 419.64 Sample Preparation Description Syringe Filters with PVDF Membrane Description Quantity Diameter Pore Size Acrodisc® LC pkg/50 case/200 pkg/100 case/300 pkg/100 case/300 pkg/100 case/300 pkg/50 case/200 pkg/100 case/300 25 mm 25 mm 13 mm 13 mm 13 mm 13 mm 13 mm 13 mm 25 mm 25 mm 13 mm 13 mm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.2 µm 0.2 µm 0.2 µm 0.2 µm 0.2 µm 0.2 µm Acrodisc® LC Minispike Acrodisc® LC Acrodisc® LC Minispike Part No. Price WAT200510 WAT200511 WAT200512 WAT200513 WAT200560 WAT200561 WAT200806 WAT200807 WAT200808 WAT200809 WAT200804 WAT200805 110.00 382.43 150.00 398.00 155.00 407.52 155.00 409.00 110.00 385.51 158.00 409.00 Syringe Filters with GHP Membrane Description Quantity Diameter Pore Size GHP Acrodisc® pkg/50 case/200 pkg/50 case/200 pkg/100 case/300 pkg/50 case/200 pkg/100 pkg/300 25 mm 25 mm 25 mm 25 mm 13 mm 13 mm 25 mm 25 mm 13 mm 13 mm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.2 µm 0.2 µm 0.2 µm 0.2 µm GHP Acrodisc® GF GHP Acrodisc® Minispike GHP Acrodisc® GHP Acrodisc® Minispike Part No. Price WAT200514 WAT200515 WAT200802 WAT200803 WAT200516 WAT200517 WAT097964 WAT097965 WAT097962 WAT097963 120.00 382.00 117.00 405.33 165.00 420.00 120.00 398.00 165.00 420.00 © 2001 Waters Corporation 47 Sample Preparation Syringe Filters with Nylon Membrane Description Nylon Acrodisc ® Nylon Acrodisc® GF Nylon Acrodisc® Minispike Nylon Acrodisc® Nylon Acrodisc® Minispike Quantity Diameter Pore Size pkg/50 case/200 pkg/100 case/300 pkg/250 case/750 pkg/50 case/200 pkg/100 case/300 pkg/50 case/200 pkg/100 case/300 pkg/100 case/300 25 mm 25 mm 13 mm 13 mm 4 mm 4 mm 25 mm 25 mm 13 mm 13 mm 25 mm 25 mm 13 mm 13 mm 13 mm 13 mm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.2 µm 0.2 µm 0.2 µm 0.2 µm 0.2 µm 0.2 µm Part No. Price WAT200518 WAT200519 WAT200520 WAT200521 WAT200526 WAT200527 WAT200800 WAT200801 WAT200564 WAT200565 WAT200522 WAT200523 WAT200524 WAT200525 WAT200562 WAT200563 118.00 384.00 149.00 408.96 265.00 736.81 116.00 401.86 155.00 407.52 118.00 384.00 149.00 408.96 155.00 407.52 Part No. Price WAT200528 WAT200529 86.00 86.00 Part No. Price WAT200812 WAT200813 WAT200816 WAT200817 WAT200810 WAT200811 WAT200814 WAT200815 169.00 435.00 122.00 419.00 169.00 435.00 122.00 419.00 Part No. Price WAT200818 WAT200819 63.00 226.00 Sterile Syringe Filters with Polyethersulfone Membrane Description Quantity Supor Acrodisc ® pkg/50 ® Diameter Pore Size 25 mm 0.45 µm 0.2 µm Syringe Filters with Supor® Membrane Description Quantity Diameter Pore Size IC Acrodisc pkg/100 case/300 pkg/50 case/200 pkg/100 case/300 pkg/50 case/200 13 mm 13 mm 25 mm 25 mm 13 mm 13 mm 25 mm 25 mm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.2 µm 0.2 µm 0.2 µm 0.2 µm ® Syringe Filters with Glass Fiber Membrane Description Glass Fiber Acrodisc ® Quantity Diameter Pore Size pkg/50 case/200 25 mm 25 mm 1.0 µm 1.0 µm © 2001 Waters Corporation 48 Sample Preparation Special Bulk Packaging (1000 devices) Description Diameter Qty Part No. Price 0.45 0.45 0.2 0.2 0.2 0.45 1000 1000 1000 1000 1000 1000 WAT200820 WAT200821 WAT200822 WAT200823 WAT200824 WAT200825 998.00 772.00 998.00 772.00 772.00 772.00 25 mm 13 mm 13 mm 13 mm 25 mm 0.45 0.45 0.45 0.2 0.2 1000 1000 1000 1000 1000 WAT200826 WAT200827 WAT200828 WAT200838 WAT200839 998.00 772.00 772.00 772.00 998.00 25 mm 25 mm 13 mm 0.45 0.45 0.45 1000 1000 1000 WAT058853 WAT200829 WAT200830 1,050.00 1,008.00 780.00 Sample Preparation Pore Size Syringe Filters with PTFE Membrane Acrodisc® CR Acrodisc® CR Acrodisc® CR Acrodisc® CR Acrodisc® CR Minispike Acrodisc® CR Minispike 25 mm 13 mm 25 mm 13 mm 13 mm 13 mm Syringe Filters with PVDF Membrane Acrodisc® LC Acrodisc® LC Acrodisc® LC Minispike Acrodisc® LC Minispike Acrodisc® LC Syringe Filters with GHP Membrane GHP Acrodisc® GF GHP Acrodisc® GHP Acrodisc® Minispike Syringe Filters with Nylon Membrane Nylon Acrodisc® Nylon Acrodisc® Nylon Acrodisc® Nylon Acrodisc® Nylon Acrodisc® Minispike Nylon Acrodisc® Minispike Nylon Acrodisc® GF 25 mm 13 mm 25 mm 13 mm 13 mm 13 mm 25 mm 0.45 0.45 0.2 0.2 0.2 0.45 0.45 1000 1000 1000 1000 1000 1000 1000 WAT200831 WAT200832 WAT200833 WAT200834 WAT200835 WAT200836 WAT200846 998.00 772.00 998.00 772.00 772.00 772.00 1,040.00 Glass Fibre Acrodisc® Versapor Acrodisc® IC Acrodisc® IC Acrodisc® IC Acrodisc® IC Acrodisc® 25 mm 25 mm 13 mm 25 mm 13 mm 25 mm 1 0.8 0.45 0.45 0.2 0.2 1000 1000 1000 1000 1000 1000 WAT200840 WAT200841 WAT200842 WAT200843 WAT200844 WAT200845 954.00 1,050.00 806.00 1,060.00 806.00 1,060.00 TM Visit Waters on-line product resource today © 2001 Waters Corporation 49 Sample Preparation Solvent Filtration Membranes Description Quantity Diameter Pore Size PVDF Filter Nylon Filter PTFE Filter pkg/100 pkg/100 pkg/100 pkg/100 pkg/100 pkg/100 pkg/100 pkg/100 pkg/100 pkg/100 pkg/100 pkg/100 pkg/100 47 mm 47 mm 47 mm 13 mm 47 mm 47 mm 13 mm 47 mm 47 mm 47 mm 47 mm 10 mm 10 mm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.2 µm 0.2 µm 0.2 µm 0.2 µm 1.0 µm 1.0 µm GH Polypro Filter Supor® (PES) Filter PVDF Filter Nylon Filter PTFE Filter Supor® (PES) Filter Thick Glass Filter A/E Glass Filter Part No. Price WAT200530 WAT200532 WAT200534 WAT200536 WAT200537 WAT200538 WAT200540 WAT200531 WAT200533 WAT200535 WAT200539 WAT200541 WAT200542 86.00 86.00 231.00 140.00 92.00 82.00 51.00 86.00 86.00 231.00 82.00 20.00 22.00 Solvent Filtration Apparatus The 300 mL capacity 47 mm Glass Filter Funnel and 1 litre capacity 47 mm Glass Funnel/Support Assembly are ideal for vacuum filtration of liquids and degassing of HPLC solvent and mobile phases. The 100% borosilicate glass construction assures resistance to even the most aggressive solvents. Description All Glass Filter Holder 47mm, complete Forceps, SS Funnel, 300 mL Glass Base, tabulated cap Ground Joint Flask Swinney Holder Part No. Price WAT200543 WAT200544 WAT200545 WAT200546 WAT200547 WAT200566 400.00 20.00 73.00 165.00 113.00 55.00 © 2001 Waters Corporation 50
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