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GE Healthcare
Protein and nucleic acid
sample preparation
Selection Guide
imagination at work
Nucleic Acid and Protein Workflows
Selection Guide for Protein Sample Preparation
NHS HP SpinTrap™
Sample(s): blood, tissue, cells, bacteria, yeast, etc.
RNA Preparation
Plasmid DNA Preparation
Genomic DNA Preparation
Lysis/Protein Extraction
Enzyme Regulation
Untagged Protein Enrichment Code No.
28-9031-28
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Protein Enrichment and Immunoprecipitation
Plasmid Amplification
RNA Purification
Optional Steps
High-abundance Protein Depletion
Genomic DNA Purification
Desalting/Buffer Exchange/Cleanup
Whole Genome Amplification
PCR
RT-PCR
PCR
DNA Cleanup
DNA Cleanup
DNA Cleanup
2-D Electrophoresis/DIGE
Optional Steps
Gene Expression
or
Concentration
Cloning & Sequencing
Biomolecule of Interest: e.g., Biomarker
Construction of
expression-ready
DNA clones
Overexpression and purification
of specific protein(s),
structure and function studies
Sample(s): cells, bacteria, yeast, etc.
Optional Steps
Plasmid DNA Preparation
Enzyme Regulation
Cloning & Sequencing
Protein Expression
1-D Electrophoresis
Protein Capture and Cleavage of Tag
Liquid Chromatography
Desalting/Buffer Exchange/Cleanup
Concentration
Protein Interaction Analysis
Characterized Biomolecule: e.g., Biomarker
Mass Spectrometry
imagination at work
Concentration
Optional Steps
Desalting/Buffer Exchange/Cleanup
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Protease inhibitor cocktail
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Cocktail of nucleases for the removal of DNA and RNA from the protein sample
80-6501-04
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Fractionation of the total protein in the sample into six discrete fractions
Magnetic beads for coupling of an affinity molecule with a primary amine,
pull-down applications
NHS HP SpinTrap Buffer Kit
28-9135-69
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Buffers for immuno-affinity enrichment using NHS HP SpinTrap
2-D Fractionation Kit
Streptavidin HP SpinTrap
28-9031-30
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Coupling of a biotinylated affinity molecule, spin column format
Total Protein Quantitation
Streptavidin HP SpinTrap Buffer Kit
28-9135-68
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Buffers for immuno-affinity enrichment using Streptavidin HP SpinTrap
2-D Quant Kit
80-6483-56
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Streptavidin HP MultiTrap™
28-9031-31
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Coupling of a biotinylated affinity molecule in 96-well filter plates by
vacuum or centrifuge, larger sample series or automation
NanoVue™
28-9232-16
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Protein A HP SpinTrap
28-9031-32
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Coupling of an IgG affinity molecule, spin column format
Protein A HP MultiTrap
28-9031-33
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Coupling of an IgG affinity molecule in 96-well filter plates by vacuum or
centrifuge, large sample series or automation
Protein A Mag Sepharose
28-9440-06
28-9513-78
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Protein G HP SpinTrap
28-9031-34
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28-9135-67
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Fractionation
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HiTrap™ Albumin & IgG Depletion
Albumin & IgG Depletion SpinTrap
28-9466-03
28-9480-20
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Prepacked 1 ml column for the depletion of albumin and IgG from larger
sample volumes (~150 µl) of human plasma and serum, for use with a
syringe or chromatography system such as ÄKTA design
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Depletion of albumin and IgG from smaller sample volumes (~50 µl) of
human plasma and serum, spin column format
Lysis/Protein Extraction
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Sample Grinding Kit
80-6483-37
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The mechanical breaking up of cells or tissue releasing the total protein
content
Drop. Measure. Done. Spectrophotometer handling 0.5 to 2 μl sample volume
Yeast Protein
Extraction Buffer Kit
28-9440-45
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Extraction of proteins from yeast cells
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Quantitation of protein amount using a colorimetric assay
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Code no.
Pack size
FTA Cards for DNA Collection, Purifcation, and Analysis
WB120067
WB120205
FTA Kit includes 25 FTA Micro Cards, 50 mL FTA Purification Reagent, and
2 Harris Uni-Core Punches with cutting mat and instructions
FTA Classic Card with 4 sample areas per card
100/pk
WB120055
FTA Mini Card with 2 sample areas per card
100/pk
WB120210
FTA Micro Card with 1 sample area per card
100/pk
FTA Gene Card with 3 sample areas per card
100/pk
Indicating FTA Cards for DNA Collection, Purification, and Analysis
WB120206
Indicating FTA Classic Card with 4 sample areas per card
100/pk
WB120056
Indicating FTA Mini Card with 2 sample areas per card
100/pk
WB120211
Indicating FTA Micro Card with 1 sample area per card
100/pk
FTA Elute Cards for DNA Collection and Purification
WB120410
FTA Elute Micro Card with 4 sample areas per card
His GraviTrap™
11-0033-99
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Purification of histidine-tagged proteins, gravity flow column
Mammalian Protein Extraction Buffer 28-9412-79
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Extraction of proteins from mammalian cells
WB120401
FTA Elute Micro Card with 4 sample areas per card
25/pk
His GraviTrap Kit
28-4013-51
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Gravity flow columns for histidine-tagged protein purification and buffers
28-9435-22
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Indicating FTA Elute Micro Card with 1 sample area per card
100/pk
Magnetic beads for coupling of an IgG affinity molecule,
immunoprecipitation applications
28-4009-89
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Purification of histidine-tagged proteins in 96-well filter plates by vacuum
or centrifuge, for larger sample series or automation
Protein extraction from tissues and cell lines, trial kit with six different
buffers for optimization of the method
WB120411
His MultiTrap HP
2-D Protein Extraction
Buffer Trial Kit
WB120412
Indicating FTA Elute Micro Card with 1 sample area per card
25/pk
2-D Protein Extraction Buffer-I
28-9435-23
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Protein extraction buffer - larger volume of Buffer-I from the trial kit
Plasimd Archiving and Analysis
Coupling of an IgG affinity molecule, spin column format
His MultiTrap FF
28-4009-90
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Purification of histidine-tagged proteins in 96-well filter plates by vacuum
or centrifuge, for larger sample series or automation
Buffers for immuno-affinity enrichment using the Protein A HP SpinTrap,
Protein G HP SpinTrap, and Ab SpinTrap
Histidine-tagged Protein Capture
His SpinTrap
28-4013-53
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Purification of histidine-tagged proteins, spin column format
His SpinTrap Kit
28-9321-71
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Microspin columns for histidine-tagged protein purification and buffers
2-D Protein Extraction Buffer-II
28-9435-24
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Protein extraction buffer - larger volume of Buffer-II from the trial kit
WB120028
CloneSaver 96-well format
5/pk
28-9435-25
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Protein extraction buffer - larger volume of Buffer-III from the trial kit
WB120052
CloneSaver Starter Kit, 96-well format
2/pk
SPOT CloneSaver Holder for sample application by a liquid handler
1/pk
2-D Protein Extraction Buffer-IV
28-9435-26
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Protein extraction buffer - larger volume of Buffer-IV from the trial kit
WB100034
28-9435-27
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Protein extraction buffer - larger volume of Buffer-V from the trial kit
Plant DNA Collection, Purifcation, and Analysis
Buffers for the purification of histidine-tagged proteins using the
His GraviTrap, HisTrap™, or His SpinTrap
2-D Protein Extraction Buffer-V
2-D Protein Extraction Buffer-VI
28-9435-28
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Protein extraction buffer - larger volume of Buffer-VI from the trial kit
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Unconjugated antibody with affinity for the histidine-tag
illustra™ triplePrep kit
28-9425-44
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Extraction of DNA, RNA, and protein from the same undivided sample
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Simple purification with a syringe or chromatography system such as
ÄKTA™ design
Protein Concentration
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Simple purification with a syringe or chromatography system such as
ÄKTA design
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Coupling of an IgG affinity molecule in 96-well filter plates by vacuum or
centrifuge, larger sample series or automation
His Buffer Kit
11-0034-00
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Protein G Mag Sepharose
28-9440-08
28-9513-79
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Magnetic beads for coupling of an IgG affinity molecule,
immunoprecipitation applications
Anti-His antibody
27-4710-01
Ab SpinTrap
28-4083-47
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Coupling of an IgG affinity molecule, spin column format, larger pack size
HisTrap HP columns
17-5247-01
Immunoprecipitation Starter Pack
17-6002-35
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Coupling of an IgG affinity molecule, batch application
Phos SpinTrap Fe
28-9298-81
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Enrichment of phosphorylated peptides, spin column format, IMAC
precharged with Fe
HisTrap FF columns
17-5319-01
HisPrep™ FF 16/10 column
17-5256-01
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Easy scale-up purification of histidine-tagged proteins
TiO2 Mag Sepharose
28-9440-10
28-9513-77
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Magnetic beads, titanium dioxide chromatography for enrichment of
phophorylated peptides
Vivaspin 500, 10 kDa MWCO PES
28-9322-25
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HisTrap FF crude Kit
28-4014-77
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Vivaspin 500, 30 kDa MWCO PES
28-9322-35
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MagRack 6
28-9489-64
Magnetic rack designed for Mag Sepharose products, enables preparation
of up to six samples in 1.5 ml microcentrifuge tubes
Purification with a syringe or chromatography system (e.g., ÄKTA design), no
filtration of the sample
Maltose Binding Protein (MBP)-tagged Protein Capture
Vivaspin 500, 50 kDa MWCO PES
28-9322-36
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Concentration of up to 500 μl sample through ultrafiltration, 50 kDa cut-off
WB120204
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Simple purification with a syringe or chromatography system such as ÄKTA design
Vivaspin 500, 100 kDa MWCO PES
28-9322-37
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Concentration of up to 500 μl sample through ultrafiltration, 100 kDa cut-off
Harris Micro Punch
Strep(II)-tagged Protein Capture
Vivaspin 2, 3 kDa MWCO PES
28-9322-40
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Concentration of up to 2 ml sample through ultrafiltration, 3 kDa cut-off
Simple purification with a syringe or chromatography system such as ÄKTA design
Vivaspin 2, 5 kDa MWCO PES
28-9322-45
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Concentration of up to 2 ml sample through ultrafiltration, 5 kDa cut-off
Vivaspin 2, 10 kDa MWCO PES
28-9322-47
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Concentration of up to 2 ml sample through ultrafiltration, 10 kDa cut-off
MBPTrap™ HP
Protein A HP SpinTrap
28-9031-32
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Purification of IgG, spin column format
Protein A HP MultiTrap
28-9031-33
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Purification of IgG in 96-well filter plates by vacuum or centrifuge, larger
sample series or automation
StrepTrap™ HP
GST Detection Module
Protein G HP SpinTrap
28-9031-34
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Purification of IgG, spin column format
Protein G HP MultiTrap
28-9031-35
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Purification of IgG in 96-well filter plates by vacuum or centrifuge, larger
sample series or automation
Ab SpinTrap
28-4083-47
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Purification of IgG, spin column format, larger pack size
Ab Buffer Kit
28-9030-59
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Buffers for antibody purification using the Protein A HP SpinTrap,
Protein G HP SpinTrap, and Ab SpinTrap
Desalting/Buffer Exchange/Cleanup
Disposable PD-10 Desalting
17-0851-01
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Clean-up of proteins/oligosaccharides, sample volumes up to 2.5 ml by
gravity flow and column spin flow
LabMate™ PD-10 Buffer
18-3216-03
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Buffer reservoir for easy equilibration
PD MidiTrap™ G-25
28-9180-08
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Clean-up of proteins/oligosaccharides, sample volumes up to 1.0 ml, by
gravity flow and spin flow
28-9180-07
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Clean-up of proteins/oligosaccharides, sample volumes up to 0.5 ml, by
gravity flow and spin flow
28-9187-78
28-9075-46
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GST-tagged Protein Capture
27-4590-01
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Reagents for the biochemical reaction performed by the GST-tag for the
determination of concentration
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Concentration of up to 500 μl sample through ultrafiltration, 3 kDa cut-off
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Concentration of up to 500 μl sample through ultrafiltration, 5 kDa cut-off
SWB120220
FTA Concentrator-PS Sample Kit
5/pk
WB120220
FTA Concentrator-PS
50/pk
Concentration of up to 500 μl sample through ultrafiltration, 10 kDa cut-off
WB120070
Disposable Filter Funnel, Grade 4
5/pk
Concentration of up to 500 μl sample through ultrafiltration, 30 kDa cut-off
FTA Purification Reagent
Vivaspin 2, 30 kDa MWCO PES
28-9322-48
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Concentration of up to 2 ml sample through ultrafiltration, 30 kDa cut-off
Vivaspin 2, 50 kDa MWCO PES
28-9322-57
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Concentration of up to 2 ml sample through ultrafiltration, 50 kDa cut-off
FTA Purification Reagent
500 mL
WB100005
Harris Micro Punch 1.2 mm (with cutting mat)
1/pk
WB100007
Harris Micro Punch 2.0 mm (with cutting mat)
1/pk
WB100038
Harris Micro Punch 3.0 mm (with cutting mat)
1/pk
WB100025
Harris Micro Punch 1.2 mm Replacement Plunger
1/pk
WB100026
Harris Micro Punch 2.0 mm Replacement Plunger
1/pk
WB100041
Harris Micro Punch 3.0 mm Replacement Plunger
1/pk
Vivaspin 2, 100 kDa MWCO PES
28-9322-58
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Concentration of up to 2 ml sample through ultrafiltration, 100 kDa cut-off
WB100006
Replacement Tip 1.2 mm
1/pk
Vivaspin 6, 3 kDa MWCO PES
28-9322-93
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Concentration of up to 6 ml sample through ultrafiltration, 3 kDa cut-off
WB100008
Replacement Tip 2.0 mm
1/pk
GSTrap™ HP columns
17-5281-01
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Simple purification with a syringe or chromatography system such as ÄKTA design
Vivaspin 6, 5 kDa MWCO PES
28-9322-94
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Concentration of up to 6 ml sample through ultrafiltration, 5 kDa cut-off
WB100042
Replacement Tip 3.0 mm
1/pk
GSTrap FF columns
17-5130-02
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Simple purification with a syringe or chromatography system such as ÄKTA design
Vivaspin 6, 10 kDa MWCO PES
28-9322-96
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Concentration of up to 6 ml sample through ultrafiltration, 10 kDa cut-off
WB100020
Replacement cutting mat
1/pk
GSTrap 4B columns
28-4017-45
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Simple purification with a syringe or chromatography system such as ÄKTA design
Vivaspin 6, 30 kDa MWCO PES
28-9323-17
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Concentration of up to 6 ml sample through ultrafiltration, 30 kDa cut-off
Disposable Harris Uni-Core Punch
GST GraviTrap
28-9536-36
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Purification of GST-tagged proteins in gravity flow format
Vivaspin 6, 50 kDa MWCO PES
28-9323-18
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Concentration of up to 6 ml sample through ultrafiltration, 50 kDa cut-off
GST MultiTrap FF
28-4055-01
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Purification of GST-tagged proteins in 96-well filter plates by vacuum or
centrifuge, for larger sample series or automation
Vivaspin 6, 100 kDa MWCO PES
28-9323-19
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Concentration of up to 6 ml sample through ultrafiltration, 100 kDa cut-off
Vivaspin 20, 3 kDa MWCO PES
28-9323-58
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Concentration of up to 20 ml sample through ultrafiltration, 3 kDa cut-off
WB100028
Harris Uni-Core Punch 1.2 mm
4/pk
WB100029
Harris Uni-Core Punch 2.0 mm
4/pk
WB100039
Harris Uni-Core Punch 3.0 mm
4/pk
WB100040
Harris Uni-Core Punch 6.0 mm
4/pk
GST MultiTrap 4B
28-4055-00
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Purification of GST-tagged proteins in 96-well filter plates by vacuum or
centrifuge, for larger sample series or automation
Vivaspin 20, 5 kDa MWCO PES
28-9323-59
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Concentration of up to 20 ml sample through ultrafiltration, 5 kDa cut-off
GST SpinTrap
28-9536-34
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Purification of GST-tagged proteins, spin column format
Vivaspin 20, 10 kDa MWCO PES
28-9323-60
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Concentration of up to 20 ml sample through ultrafiltration, 10 kDa cut-off
WB100036
Multi-Barrier Pouch, Small (8 × 7 cm)
100/pk
Vivaspin 20, 30 kDa MWCO PES
28-9323-61
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Concentration of up to 20 ml sample through ultrafiltration, 30 kDa cut-off
WB100037
Multi-Barrier Pouch, Large (9 × 15 cm)
100/pk
CloneSaver Resealable Multi-Barrier Pouch
50/pk
Clean-up of proteins/oligosaccharides, sample volumes up to 130 μl, by
microcentrifugation
GST Buffer Kit
28-9523-61
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Buffers for purification of GST-tagged proteins using GSTrap, GST GraviTrap,
or GST SpinTrap
PD MultiTrap G-25
28-9180-06
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Clean-up of proteins/oligosaccharides, sample volumes up to 130 μl in
parallel, 96-well filter plates by centrifuge, large sample series, and automation
pGEX vectors
multiple
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Expression vectors for GST fusion proteins
Peptide and small protein applications, clean-up sample volumes up to
800 μl, by gravity flow
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Simple purification with a syringe or chromatography system such as ÄKTA design
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17-5234-01
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GSTPrep™ FF 16/10 column
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100/pk
Parasite DNA Collection, Purification, and Analysis
Reagents and plates with precoated wells for the detection of
GST-tagged proteins
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1/pk
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28-9322-18
28-9322-23
WB120065
FTA Plant Kit includes 20 FTA PlantSaver Cards, 2.0 mm Uni-Core Punch, 1 cutting mat,
50 mL FTA Purification Reagent, 1 pair Nitrile Gloves, and 1 round bottom test tube for
sample application and instructions
FTA PlantSaver Card with 4 sample areas per card
27-4592-01
28-9180-04
28-9180-11
Vivaspin 500, 3 kDa MWCO PES
Vivaspin 500, 5 kDa MWCO PES
WB120068
GST 96-well Detection Module
PD SpinTrap G-25
PD MidiTrap G-10
100/pk
2-D Protein Extraction Buffer-III
28-9031-35
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1/pk
WB120208
Protein G HP MultiTrap
PD MiniTrap™ G-25
Lysis/Protein Extraction
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Small-scale Antibody Purification
Mass Spectrometry
80-6501-23
80-6501-42
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1-D Electrophoresis/Western Blotting
Buffer Exchange/Cleanup
Genotyping
Cloning & Sequencing
Liquid Chromatography
Protease Inhibitor Mix
Nuclease Mix
28-9440-09
28-9513-80
Protein A/G HP SpinTrap Buffer Kit
Concentration
Choose analysis method
Protein Depletion
NHS Mag Sepharose
Enzyme Regulation
Plasmid DNA Purification
Bulk NHS HP Sepharose™ and empty spin columns for coupling of an
affinity molecule with a primary amine
Protein Sample Preparation
2D
Differential Expression Analysis
Protein Mapping
Choose analysis method
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Protein Workflow
Genome and Gene Analysis
Protein Sample Preparation
2D
Nucleic Acid Workflow
Choose analysis method
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Protein Sample Preparation
Whatman™ products
GST Vector primers for
sequencing
multiple
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Sequencing primers for the pGEX vectors
E. coli BL21
27-1542-01
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Bacterial strain for the expression of GST fusion protein
Multi-Barrier Pouch
Vivaspin 20, 50 kDa MWCO PES
28-9323-62
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Concentration of up to 20 ml sample through ultrafiltration, 50 kDa cut-off
WB100024
Vivaspin 20, 100 kDa MWCO PES
28-9323-63
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Concentration of up to 20 ml sample through ultrafiltration, 100 kDa cut-off
Dessicant Packets
WB100003
Dessicant Packets (1 g)
1000/pk
FTA Gene Card Tray
PD MiniTrap G-10
28-9180-10
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Peptide and small protein applications, clean-up sample volumes up to
300 μl, by gravity flow
M13K07 Helper Phage
27-1524-01
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M13 phage for the GST expression system
WB100030
Mini Dialysis Kit – 1 kDa cut-off
80-6483-75
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Dialysis of small sample volumes, 250 μl and 2 ml
GST Bulk Kit
27-4570-01
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Bulk Glutathine Sepharose 4B and empty gravity columns
Buccal Cell Collection
Mini dialysis kit - 1 kDa cut off
80-6483-94
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Dialysis of small sample volumes, max. sample volume 2 ml
PreScission™ Protease
27-0843-01
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Purified enzyme for site-specific cleavage of the GST-tag from fusion
proteins expressed using the pGEX-P vectors
WB120462
EasiCollect with 1 sample area per card
50/pk
WB100032
Sterile Foam Tipped Applicators for Transfer of Buccal Cells to FTA Cards
100/pk
Thrombin
27-0846-01
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Purified enzyme for site-specific cleavage of the GST-tag from fusion
proteins expressed using the pGEX-T vectors
WB100035
Sterile Omni Swab
100/pk
Mini Dialysis Kit – 8 kDa cut-off
80-6484-13
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Dialysis of small sample volumes, 250 μl and 2 ml
Mini dialysis kit - 8 kDa cut off
80-6484-32
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Dialysis of small sample volumes, max. sample volume 2 ml
2-D Clean-Up Kit
80-6484-51
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Removal of interfering contaminants and concentration of total protein
SDS-PAGE Clean-Up Kit
80-6484-70
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Removal of interfering contaminants
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For more information on protein sample preparation, please visit www.gelifesciences.com/sampleprep
Factor Xa
27-0849-01
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Purified enzyme for site-specific cleavage of the GST-tag from fusion
proteins expressed using the pGEX-X vectors
Anti-GST Antibody
27-4577-01
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Unconjugated antibody with affinity for the GST-tag
For more information on protein sample preparation at larger scale, please visit www.gelifesciences.com/protein-purification
FTA Gene Card Tray holds 2 Gene Cards for sample application by a liquid handler
20/pk
Blood Stain Cards
WB100014
For more information on protein sample preparation, please visit www.gelifesciences.com/sampleprep
Blood Stain Cards
For more information on Whatman products, please visit www.whatman.com
100/pk
Selection Guide for Protein Sample P
Protein Sample Preparation
2D
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Choose analysis method
Untagged Protein Enrichment Code No.
NHS HP SpinTrap™
28-9031-28
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•
Bulk NHS HP Sepharose™ and empty spin columns for coupling of an
affinity molecule with a primary amine
NHS Mag Sepharose
28-9440-09
28-9513-80
•
•
Magnetic beads for coupling of an affinity molecule with a primary amine,
pull-down applications
NHS HP SpinTrap Buffer Kit
28-9135-69
•
•
Buffers for immuno-affinity enrichment using NHS HP SpinTrap
Streptavidin HP SpinTrap
28-9031-30
•
•
Coupling of a biotinylated affinity molecule, spin column format
Streptavidin HP SpinTrap Buffer Kit
28-9135-68
•
•
Buffers for immuno-affinity enrichment using Streptavidin HP SpinTrap
Streptavidin HP MultiTrap™
28-9031-31
•
•
Coupling of a biotinylated affinity molecule in 96-well filter plates by
vacuum or centrifuge, larger sample series or automation
Protein A HP SpinTrap
28-9031-32
•
•
Coupling of an IgG affinity molecule, spin column format
Protein A HP MultiTrap
28-9031-33
•
•
Coupling of an IgG affinity molecule in 96-well filter plates by vacuum or
centrifuge, large sample series or automation
Protein A Mag Sepharose
28-9440-06
28-9513-78
•
•
Magnetic beads for coupling of an IgG affinity molecule,
immunoprecipitation applications
Protein G HP SpinTrap
28-9031-34
•
•
Coupling of an IgG affinity molecule, spin column format
Protein A/G HP SpinTrap Buffer Kit
28-9135-67
•
•
Buffers for immuno-affinity enrichment using the Protein A HP SpinTrap,
Protein G HP SpinTrap, and Ab SpinTrap
Protein G HP MultiTrap
28-9031-35
•
•
Coupling of an IgG affinity molecule in 96-well filter plates by vacuum or
centrifuge, larger sample series or automation
Protein G Mag Sepharose
28-9440-08
28-9513-79
•
•
Magnetic beads for coupling of an IgG affinity molecule,
immunoprecipitation applications
Coupling of an IgG affinity molecule, spin column format, larger pack size
Ab SpinTrap
28-4083-47
•
•
Immunoprecipitation Starter Pack
17-6002-35
•
•
Coupling of an IgG affinity molecule, batch application
Phos SpinTrap Fe
28-9298-81
•
Enrichment of phosphorylated peptides, spin column format, IMAC
precharged with Fe
TiO2 Mag Sepharose
28-9440-10
28-9513-77
•
Magnetic beads, titanium dioxide chromatography for enrichment of
phophorylated peptides
MagRack 6
28-9489-64
•
Magnetic rack designed for Mag Sepharose products, enables preparation
of up to six samples in 1.5 ml microcentrifuge tubes
Small-scale Antibody Purification
Protein A HP SpinTrap
28-9031-32
•
Purification of IgG, spin column format
Protein A HP MultiTrap
28-9031-33
•
Purification of IgG in 96-well filter plates by vacuum or centrifuge, larger
sample series or automation
Protein A Mag Sepharose Xtra
28-9670-56
28-9670-62
•
Magnetic beads for small-scale purification/screening of monoclonal and
polyclonal antibodies from various species
Protein G HP SpinTrap
28-9031-34
•
Purification of IgG, spin column format
Protein G HP MultiTrap
28-9031-35
•
Purification of IgG in 96-well filter plates by vacuum or centrifuge, larger
sample series or automation
Protein G Mag Sepharose Xtra
28-9670-66
28-9670-70
•
Magnetic beads for small-scale purification/screening of monoclonal and
polyclonal antibodies from various species
Ab SpinTrap
28-4083-47
•
Purification of IgG, spin column format, larger pack size
Ab Buffer Kit
28-9030-59
•
Buffers for antibody purification using the Protein A HP SpinTrap,
Protein G HP SpinTrap, and Ab SpinTrap
Desalting/Buffer Exchange/Cleanup
Disposable PD-10 Desalting
17-0851-01
•
•
•
•
•
•
Clean-up of proteins/oligosaccharides, sample volumes up to 2.5 ml by
gravity flow and column spin flow
LabMate™ PD-10 Buffer
18-3216-03
•
•
•
•
•
•
Buffer reservoir for easy equilibration
PD MidiTrap™ G-25
28-9180-08
•
•
•
•
•
•
Clean-up of proteins/oligosaccharides, sample volumes up to 1.0 ml, by
gravity flow and spin flow
PD MiniTrap™ G-25
28-9180-07
•
•
•
•
•
•
Clean-up of proteins/oligosaccharides, sample volumes up to 0.5 ml, by
gravity flow and spin flow
PD SpinTrap G-25
28-9180-04
•
•
•
•
•
•
Clean-up of proteins/oligosaccharides, sample volumes up to 130 μl, by
microcentrifugation
PD MultiTrap G-25
28-9180-06
•
•
•
•
•
•
Clean-up of proteins/oligosaccharides, sample volumes up to 130 μl in
parallel, 96-well filter plates by centrifuge, large sample series, and automation
PD MidiTrap G-10
28-9180-11
•
•
•
•
•
•
Peptide and small protein applications, clean-up sample volumes up to
800 μl, by gravity flow
PD MiniTrap G-10
28-9180-10
•
•
•
•
•
•
Peptide and small protein applications, clean-up sample volumes up to
300 μl, by gravity flow
Mini Dialysis Kit – 1 kDa cut-off
80-6483-75
•
•
•
•
•
•
Dialysis of small sample volumes, 250 μl and 2 ml
Mini dialysis kit - 1 kDa cut off
80-6483-94
•
•
•
•
•
•
Dialysis of small sample volumes, max. sample volume 2 ml
Mini Dialysis Kit – 8 kDa cut-off
80-6484-13
•
•
•
•
•
•
Dialysis of small sample volumes, 250 μl and 2 ml
Mini dialysis kit - 8 kDa cut off
80-6484-32
•
•
•
•
•
•
Dialysis of small sample volumes, max. sample volume 2 ml
2-D Clean-Up Kit
80-6484-51
•
SDS-PAGE Clean-Up Kit
80-6484-70
•
•
Removal of interfering contaminants and concentration of total protein
•
Removal of interfering contaminants
For more information on protein sample preparation, please visit www.gelifesciences.com/sampleprep
Preparation
Protein Sample Preparation
2D
El
1- ectr
D
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Liq lect hor
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ui
Fr d Ch pho s
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2
Fr -D ato
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Fr 1-D ho aph
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Pr LC
S
ho
ot
t
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Ma in E MS o M
S
x
ss
p
Sp res
ec sio
tro n
m
et
ry
Choose analysis method
Enzyme Regulation
Code No.
Protease Inhibitor Mix
80-6501-23
•
•
•
•
•
Protease inhibitor cocktail
Nuclease Mix
80-6501-42
•
•
•
•
•
Cocktail of nucleases for the removal of DNA and RNA from the protein sample
80-6501-04
•
•
•
•
Fractionation of the total protein in the sample into six discrete fractions
Fractionation
2-D Fractionation Kit
Total Protein Quantitation
2-D Quant Kit
80-6483-56
•
•
•
NanoVue™ Plus
28-9569-65
•
•
•
•
•
•
•
•
Quantitation of protein amount using a colorimetric assay
•
•
•
Drop. Measure. Done. Spectrophotometer handling 0.5 to 2 μl sample volume
Histidine-tagged Protein Capture
His GraviTrap™
11-0033-99
•
Purification of histidine-tagged proteins, gravity flow column
His Mag Sepharose Ni
28-9673-88
28-9673-90
28-9799-17
•
Magnetic beads for small-scale purification/screening of
histidine-tagged proteins
His GraviTrap Kit
28-4013-51
•
Gravity flow columns for histidine-tagged protein purification and buffers
His MultiTrap HP
28-4009-89
•
Purification of histidine-tagged proteins in 96-well filter plates by vacuum
or centrifuge, for larger sample series or automation
His MultiTrap FF
28-4009-90
•
Purification of histidine-tagged proteins in 96-well filter plates by vacuum
or centrifuge, for larger sample series or automation
His SpinTrap
28-4013-53
•
Purification of histidine-tagged proteins, spin column format
His SpinTrap Kit
28-9321-71
•
Microspin columns for histidine-tagged protein purification and buffers
His Buffer Kit
11-0034-00
•
Buffers for the purification of histidine-tagged proteins using the
His GraviTrap, HisTrap™, or His SpinTrap
Anti-His antibody
27-4710-01
•
Unconjugated antibody with affinity for the histidine-tag
HisTrap HP columns
17-5247-01
•
Simple purification with a syringe or chromatography system such as
ÄKTA™ design
HisTrap FF columns
17-5319-01
•
Simple purification with a syringe or chromatography system such as
ÄKTA design
HisPrep™ FF 16/10 column
17-5256-01
•
Easy scale-up purification of histidine-tagged proteins
HisTrap FF crude Kit
28-4014-77
•
Purification with a syringe or chromatography system (e.g., ÄKTA design), no
filtration of the sample
•
Simple purification with a syringe or chromatography system such as ÄKTA design
28-9075-46
•
Simple purification with a syringe or chromatography system such as ÄKTA design
GST Detection Module
27-4590-01
•
Reagents for the biochemical reaction performed by the GST-tag for the
determination of concentration
GST 96-well Detection Module
27-4592-01
•
Reagents and plates with precoated wells for the detection of
GST-tagged proteins
Maltose Binding Protein (MBP)-tagged Protein Capture
MBPTrap™ HP
28-9187-78
Strep(II)-tagged Protein Capture
StrepTrap™ HP
GST-tagged Protein Capture
GSTPrep™ FF 16/10 column
28-9365-50
•
Simple purification with a syringe or chromatography system such as ÄKTA design
GSTrap™ HP columns
17-5281-01
•
Simple purification with a syringe or chromatography system such as ÄKTA design
GSTrap FF columns
17-5130-02
•
Simple purification with a syringe or chromatography system such as ÄKTA design
GSTrap 4B columns
28-4017-45
•
Simple purification with a syringe or chromatography system such as ÄKTA design
GST GraviTrap
28-9523-60
•
Purification of GST-tagged proteins in gravity flow format
GST MultiTrap FF
28-4055-01
•
Purification of GST-tagged proteins in 96-well filter plates by vacuum or
centrifuge, for larger sample series or automation
GST MultiTrap 4B
28-4055-00
•
Purification of GST-tagged proteins in 96-well filter plates by vacuum or
centrifuge, for larger sample series or automation
GST SpinTrap
28-9523-59
•
Purification of GST-tagged proteins, spin column format
GST Buffer Kit
28-9523-61
•
Buffers for purification of GST-tagged proteins using GSTrap, GST GraviTrap,
or GST SpinTrap
pGEX vectors
multiple
•
Expression vectors for GST fusion proteins
GST Vector primers for sequencing
multiple
•
Sequencing primers for the pGEX vectors
27-1542-01
•
Bacterial strain for the expression of GST fusion protein
E. coli BL21
M13K07 Helper Phage
27-1524-01
•
M13 phage for the GST expression system
GST Bulk Kit
27-4570-01
•
Bulk Glutathine Sepharose 4B and empty gravity columns
PreScission™ Protease
27-0843-01
•
Purified enzyme for site-specific cleavage of the GST-tag from fusion
proteins expressed using the pGEX-P vectors
Thrombin
27-0846-01
•
Purified enzyme for site-specific cleavage of the GST-tag from fusion
proteins expressed using the pGEX-T vectors
Factor Xa
27-0849-01
•
Purified enzyme for site-specific cleavage of the GST-tag from fusion
proteins expressed using the pGEX-X vectors
Anti-GST Antibody
27-4577-01
•
Unconjugated antibody with affinity for the GST-tag
For more information on protein sample preparation at larger scale, please visit www.gelifesciences.com/protein-purification
Protein Sample Preparation
2D
El
1- ectr
D
E op
Liq lect hor
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ui
Fr d Ch pho s
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m sis
2
Fr -D ato
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Fr 1-D ho aph
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Elp to M y
Pr LC
S
ho
ot
t
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Ma in E MS o M
S
x
ss
p
Sp res
ec sio
tro n
m
et
ry
Choose analysis method
Protein Depletion
HiTrap™ Albumin & IgG Depletion
28-9466-03
•
•
•
Prepacked 1 ml column for the depletion of albumin and IgG from larger
sample volumes (~150 µl) of human plasma and serum, for use with a
syringe or chromatography system such as ÄKTA design
Albumin & IgG Depletion SpinTrap
28-9480-20
•
•
•
Depletion of albumin and IgG from smaller sample volumes (~50 µl) of
human plasma and serum, spin column format
Sample Grinding Kit
80-6483-37
•
•
•
•
•
The mechanical breaking up of cells or tissue releasing the total protein
content
Yeast Protein
Extraction Buffer Kit
28-9440-45
•
•
•
•
•
Extraction of proteins from yeast cells
Lysis/Protein Extraction
Mammalian Protein Extraction Buffer 28-9412-79
•
•
•
•
•
Extraction of proteins from mammalian cells
2-D Protein Extraction
Buffer Trial Kit
•
•
•
•
•
Protein extraction from tissues and cell lines, trial kit with six different
buffers for optimization of the method
28-9435-22
2-D Protein Extraction Buffer-I
28-9435-23
•
•
•
•
•
Protein extraction buffer - larger volume of Buffer-I from the trial kit
2-D Protein Extraction Buffer-II
28-9435-24
•
•
•
•
•
Protein extraction buffer - larger volume of Buffer-II from the trial kit
2-D Protein Extraction Buffer-III
28-9435-25
•
•
•
•
•
Protein extraction buffer - larger volume of Buffer-III from the trial kit
2-D Protein Extraction Buffer-IV
28-9435-26
•
•
•
•
•
Protein extraction buffer - larger volume of Buffer-IV from the trial kit
2-D Protein Extraction Buffer-V
28-9435-27
•
•
•
•
•
Protein extraction buffer - larger volume of Buffer-V from the trial kit
2-D Protein Extraction Buffer-VI
28-9435-28
•
•
•
•
•
Protein extraction buffer - larger volume of Buffer-VI from the trial kit
illustra™ triplePrep kit
28-9425-44
•
•
•
•
Extraction of DNA, RNA, and protein from the same undivided sample
Protein Concentration
Vivaspin 500, 3 kDa MWCO PES
28-9322-18
•
•
•
•
•
•
•
•
Concentration of up to 500 μl sample through ultrafiltration, 3 kDa cut-off
Vivaspin 500, 5 kDa MWCO PES
28-9322-23
•
•
•
•
•
•
•
•
Concentration of up to 500 μl sample through ultrafiltration, 5 kDa cut-off
Vivaspin 500, 10 kDa MWCO PES
28-9322-25
•
•
•
•
•
•
•
•
Concentration of up to 500 μl sample through ultrafiltration, 10 kDa cut-off
Vivaspin 500, 30 kDa MWCO PES
28-9322-35
•
•
•
•
•
•
•
•
Concentration of up to 500 μl sample through ultrafiltration, 30 kDa cut-off
Vivaspin 500, 50 kDa MWCO PES
28-9322-36
•
•
•
•
•
•
•
•
Concentration of up to 500 μl sample through ultrafiltration, 50 kDa cut-off
Vivaspin 500, 100 kDa MWCO PES
28-9322-37
•
•
•
•
•
•
•
•
Concentration of up to 500 μl sample through ultrafiltration, 100 kDa cut-off
Vivaspin 2, 3 kDa MWCO PES
28-9322-40
•
•
•
•
•
•
•
•
Concentration of up to 2 ml sample through ultrafiltration, 3 kDa cut-off
Vivaspin 2, 5 kDa MWCO PES
28-9322-45
•
•
•
•
•
•
•
•
Concentration of up to 2 ml sample through ultrafiltration, 5 kDa cut-off
Vivaspin 2, 10 kDa MWCO PES
28-9322-47
•
•
•
•
•
•
•
•
Concentration of up to 2 ml sample through ultrafiltration, 10 kDa cut-off
Vivaspin 2, 30 kDa MWCO PES
28-9322-48
•
•
•
•
•
•
•
•
Concentration of up to 2 ml sample through ultrafiltration, 30 kDa cut-off
Vivaspin 2, 50 kDa MWCO PES
28-9322-57
•
•
•
•
•
•
•
•
Concentration of up to 2 ml sample through ultrafiltration, 50 kDa cut-off
Vivaspin 2, 100 kDa MWCO PES
28-9322-58
•
•
•
•
•
•
•
•
Concentration of up to 2 ml sample through ultrafiltration, 100 kDa cut-off
Vivaspin 6, 3 kDa MWCO PES
28-9322-93
•
•
•
•
•
•
•
•
Concentration of up to 6 ml sample through ultrafiltration, 3 kDa cut-off
Vivaspin 6, 5 kDa MWCO PES
28-9322-94
•
•
•
•
•
•
•
•
Concentration of up to 6 ml sample through ultrafiltration, 5 kDa cut-off
Vivaspin 6, 10 kDa MWCO PES
28-9322-96
•
•
•
•
•
•
•
•
Concentration of up to 6 ml sample through ultrafiltration, 10 kDa cut-off
Vivaspin 6, 30 kDa MWCO PES
28-9323-17
•
•
•
•
•
•
•
•
Concentration of up to 6 ml sample through ultrafiltration, 30 kDa cut-off
Vivaspin 6, 50 kDa MWCO PES
28-9323-18
•
•
•
•
•
•
•
•
Concentration of up to 6 ml sample through ultrafiltration, 50 kDa cut-off
Vivaspin 6, 100 kDa MWCO PES
28-9323-19
•
•
•
•
•
•
•
•
Concentration of up to 6 ml sample through ultrafiltration, 100 kDa cut-off
Vivaspin 20, 3 kDa MWCO PES
28-9323-58
•
•
•
•
•
•
•
•
Concentration of up to 20 ml sample through ultrafiltration, 3 kDa cut-off
Vivaspin 20, 5 kDa MWCO PES
28-9323-59
•
•
•
•
•
•
•
•
Concentration of up to 20 ml sample through ultrafiltration, 5 kDa cut-off
Vivaspin 20, 10 kDa MWCO PES
28-9323-60
•
•
•
•
•
•
•
•
Concentration of up to 20 ml sample through ultrafiltration, 10 kDa cut-off
Vivaspin 20, 30 kDa MWCO PES
28-9323-61
•
•
•
•
•
•
•
•
Concentration of up to 20 ml sample through ultrafiltration, 30 kDa cut-off
Vivaspin 20, 50 kDa MWCO PES
28-9323-62
•
•
•
•
•
•
•
•
Concentration of up to 20 ml sample through ultrafiltration, 50 kDa cut-off
Vivaspin 20, 100 kDa MWCO PES
28-9323-63
•
•
•
•
•
•
•
•
Concentration of up to 20 ml sample through ultrafiltration, 100 kDa cut-off
For more information on protein sample preparation, please visit www.gelifesciences.com/sampleprep
Whatman™ products
Code no.
Pack size
Klari-Flex Bottle Top Filtration System for Sterile Filtration
6514-2502
KF 250 ml 0.22 PES Unit Sterile
12/pk
6514-5002
KF 500 ml 0.22 PES Unit Sterile
12/pk
6514-1002
KF 1000 ml 0.22 PES Unit Sterile
12/pk
6515-2502
KF 250 ml 0.22 PES Funl Sterile
12/pk
6515-5002
KF 500 ml 0.22 PES Funl Sterile
12/pk
6515-1002
KF 1000 ml 0.22 PES Funl Sterile
12/pk
6516-2500
KF 250 ml Bottle Sterile
24/pk
6516-5000
KF 500 ml Bottle Sterile
24/pk
6516-1000
KF 1000 ml Bottle Sterile
24/pk
Klari-Flex Bottle Top System Hardware for Sterile Filtration
6517-0001
KF Cradle Ring
1/pk
6517-0002
KF Pedestal Stand
1/pk
Polycap PES Filter Capsules for Sterile Filtration
6715-3602
Polycap 36 0.2/0.2 PES with filling bell/Sterile
1/pk
6715-7502
Polycap 36 0.2/0.2 PES with filling bell/Sterile
1/pk
6718-9502
Polycap 150 0.2/0.2 PES with filling bell/Sterile
1/pk
SPARTAN 13 mm Syringe filters
10463100
13 mm Spartan, 0.2 µm RC
100/pk
10463110
13 mm Spartan, 0.45 µm RC
100/pk
SPARTAN 30 mm Syringe filters
10463060
30 mm Spartan, 0.2 µm RC
100/pk
10463050
30 mm Spartan, 0.45 µm RC
100/pk
GD/X Syringe Filter for Lysate Clarification
6896-2502
25 mm GD/X 0.2 µm PES (Sterile)
50/pk
6896-2504
25 mm GD/X 0.45 µm PES (Sterile)
50/pk
Syringe filters for Sterile Filtration
6791-0402
4 mm Puradisc 0.2 µm PVDF (Sterile)
50/pk
6780-2502
25 mm Puradisc 0.2 µm PES (Sterile)
50/pk
6780-2504
25 mm Puradisc 0.45 µm PES (Sterile)
50/pk
Mini-UniPrep Syringeless filters for LC and LC-MS
US203NPEPES
Mini-UniPrep 0.2 µm PES (with Slit Septum Cap)
100/pk
UN203NPERC
Mini-UniPrep 0.2 µm RC (with Standard Cap)
100/pk
For more information on Whatman products, please visit www.whatman.com
tS
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Selection Guide for DNA/RNA Samp
Amplification method
PCR
Ho
PCR and RT-PCR
Starting quantity
Basic PCR (amplicon up to 3 kb)
•
•
Hot start PCR (amplicon up to 3 kb)
•
•
•
•
Long-range PCR (amplicon up to 20 kb)
RT-PCR
•
Basic RT-PCR (amplicon up to 3 kb)
•
•
Long-range RT-PCR (amplicon up to 6 kb)
Downstream applications
•
•
•
Genotyping
•
•
•
•
•
•
•
•
Cloning & sequencing
•
•
•
•
•
•
•
•
Gene expression
•
•
•
•
•
•
•
•
oS
A
GF P-IT
XP
GF CR
X 9 an
Mi 6 P d G
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C
cr
Ba
oS R
nd
Au pin
to
GS
2
5
Pr eq
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CK
an
c
NA ol t G
P- um -50
5
NA co ns
P- lum
NA 10 c ns
P- olu
Cy 25 c mn
Sc olu s
ri
m
Mi be G ns
cr
oS FX
Mi
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Mi Spi -50
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-4
00
* Ready-To-Go is a single dose room temperature stable bead format that contains all PCR or RT-PCR components. You only
need to add DNA template, primer, and water for a reaction.
Ex
DNA Cleanup
Sample types
Starting quantity
PCR products
Any size, for sequencing only
•
50 bp to 10 kb, few samples
•
100 bp to 10 kb, many samples
•
10 to 50 bp
Agarose gel slices or
enzyme removal
50 kbp to 10 bp
Sequencing reactions
12 to 25 µl
Labeled DNA fragments
> 20 bases
•
•
•
•
> 10 bases
•
100 µg sample in 100 µl or no microcentrifuge
Oligonucleotides
•
100 to 150 µl
•
0.1 to 0.5 ml
•
0.5 to 1 ml
•
1 to 2.5 ml
•
cDNA
CyDye™ labeled probes
A range of sample types
PCR, sequencing and
labeling reactions
25 to 50 µl for labeled DNA
•
10 to 100 µl for buffer exchange or desalting
•
A range of volumes and
sample sizes
•
PCR (25 to 50 µl) or labeling reaction
(25 to 50 µl) < 100 bp
•
PCR (25 to 50 µl) or labeling reaction
(50 to 75 µl) or fragment < 200 bp
•
PCR (50 to 100 µl) or labeling reaction
(75 to 100 µl) or remove primers > 24 bases
Downstream applications
Cloning & sequencing
•
•
•
•
Gene expression
•
•
•
•
Genotyping
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
For more information on protein and nucleic acid sample preparation, please visit www.gelifesciences.com/sampleprep
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ple Preparation
Sample types
Bacterial culture
pl
as
Plasmid DNA Preparation
Starting quantity
1 µl (high-throughput)
•
1 µl (low- to medium-throughput)
1 to 3 ml
•
•
25 to 500 ml
Bacterial colony
•
< 1 colony (high-throughput)
•
< 1 colony (low- to medium-throughput)
Bacteria glycerol stock
•
< 1 µl (high-throughput)
•
< 1 µl (low- to medium-throughput)
Purified DNA (small vectors)
•
> 1 ng (high-throughput)
•
> 1 ng (low- to medium-throughput)
Purified BAC/fosmid DNA
> 1 ng
Difficult templates
(GC rich, secondary structures)
0.1 to 1 ng
•
•
•
M13 phage plaque
< 1 plaque
•
•
M13 phage glycerol stock
< 1 µl
•
•
•
•
Downstream applications
Cloning
•
•
Sequencing
•
•
•
•
As
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* Can be used directly for PCR and subcloning. Addtional steps will be needed for transformation or transfection.
Sample type and format
RN
Plasmid DNA Preparation
Starting quantity
Total RNA
Cultured cells and tissue
7
Up to 200 mg tissue or 5 × 10 cells
6
Tube format
10 to 30 mg or up to 2 × 10 cells
96-well plate vacuum
10 to 30 mg or up to 2 × 10 cells
96-well plate centrifuge
30 mg or up to 1 × 10 cells
mRNA purification
1 to 5 × 10 cells or 0.5 g tissue
Eukaryotic cells or tissue
1 to 1 × 10 cells or 100 mg tissue
Eukaryotic total RNA
Total RNA or 25 mg to 1 g tissue
Downstream applications
Gene expression
•
•
6
•
7
•
7
•
7
•
•
•
•
•
•
•
leP
re
p
kit
•
tri
p
DNA, RNA and Protein Preparation
Sample type
Starting quantity
Cultured cells
0.3 to 5 × 10 cells
•
Animal tissue
1 to 20 mg tissue
•
Downstream applications
Cloning & sequencing
•
Gene expression
•
Genotyping
•
Protein analysis (1-D, 2-D, LCMS)
•
7
For more information on nucleic acid sample preparation, please visit www.gelifesciences.com/sampleprep
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Genomic DNA Preparation
Sample types
Starting quantity (DNA yield)
Animal tissue
> 300 cells (4 to 7 µg DNA yield)
•
> 300 cells (40 to 50 µg DNA yield)
5 to 50 mg
•
•
Up to 200 mg
•
Paraffin-embedded tissue
Up to 25 mg
•
or difficult samples
20 to 30 µm thick paraffin section
•
Cultured cells
> 300 cells (4 to 7 µg DNA yield)
•
> 300 cells (40 to 50 µg DNA yield)
5
Up to 5.0 × 10 cells
•
•
5
•
1 to 3 × 10
7
Up to 2.0 × 10 cells
6
•
7
3 × 10 to 1 × 10 cells
Blood
•
5 to 10 µl (4 to 7 µg DNA yield)
•
5 to 10 µl (40 to 50 µg DNA yield)
•
50 to 300 µl
•
1 ml
•
1 to 8 ml
•
10 ml
Buffy coat
•
5 to 10 µl (4 to 7 µg DNA yield)
•
5 to 10 µl (40 to 50 µg DNA yield)
•
50 to 300 µl
Nucleated red blood cells
•
10 µl (4 to 7 µg DNA yield)
•
10 µl (40 to 50 µg DNA yield)
•
10 µl
•
25 to 200 µl (tested)
Bone marrow
5 to 10 µl (4 to 7 µg DNA yield)
(suspended cells)
5 to 10 µl (40 to 50 µg DNA yield)
•
•
•
200 µl
Bacteria (Gram - and +)
•
> 300 cells (4 to 7 µg DNA yield)
•
> 300 cells (40 to 50 µg DNA yield)
Up to 4.0 × 10
Plants
•
9
•
1 cm leaf (4 to 7 µg DNA yield)
•
1 cm leaf (40 to 50 µg DNA yield)
•
1 seed (4 to 7 µg DNA yield)
•
1 seed (40 to 50 µg DNA yield)
•
Up to 1.0 g
FTA paper/Guthrie card
•
3 × 3 mm piece (4 to 7 µg DNA yield)
•
3 × 3 mm piece (40 to 50 µg DNA yield)
Buccal swab
•
Single swab
•
Single swab (4 to 7 µg DNA yield)
•
Single swab (40 to 50 µg DNA yield)
Purified genomic DNA
•
> 10 ng (4 to 7 µg DNA yield)
•
> 10 ng (40 to 50 µg DNA yield)
Downstream applications
•
•
Genotyping
•
•
•
•
•
•
•
•
•
•
•
Cloning & sequencing
•
•
•
•
•
•
•
•
•
•
•
For more information on nucleic acid sample preparation, please visit www.gelifesciences.com/sampleprep
Ordering information
illustra Nucleic Acid Purification & Amplification
Columns
Genomic DNA Preparation
Genomic DNA Purification
illustra tissue and
cells genomicPrep™ Mini Spin Kit
illustra tissue and cells genomicPrep Mini Spin Kit
illustra tissue and cells genomicPrep Midi Flow Kit
illustra blood genomicPrep Mini Spin Kit
illustra blood genomicPrep Mini Spin Kit
illustra blood genomicPrep Midi Flow Kit
illustra blood genomicPrep Midi Flow Kit
illustra bacteria genomicPrep Mini Spin Kit
illustra bacteria genomicPrep Mini Spin Kit
Nucleon™ BACC1 Genomic DNA Kit
Nucleon BACC2 Genomic DNA Kit
Nucleon BACC3 Genomic DNA Kit
Nucleon PhytoPure™
Nucleon PhytoPure
Nucleon HT (hard tissue)
Pack size
Code No.
50
28-9042-75
250
25
50
250
25
100
50
250
50
50
50
50 × 0.1 g
50 × 1.0 g
50
28-9042-76
28-9042-73
28-9042-64
28-9042-65
28-9042-61
28-9042-62
28-9042-58
28-9042-59
RPN8501
RPN8502
RPN8512
RPN8510
RPN8511
RPN8509
Whole Genome Amplification
illustra GenomiPhi™ V2 DNA Amplification Kit
illustra GenomiPhi V2 DNA Amplification Kit
illustra GenomiPhi V2 DNA Amplification Kit
illustra GenomiPhi HY DNA Amplification Kit
illustra GenomiPhi HY DNA Amplification Kit
illustra GenomiPhi HY DNA Amplification Kit
25
100
500
25
100
1000
25-6600-30
25-6600-31
25-6600-32
25-6600-22
25-6600-20
25-6600-25
Plasmid DNA Preparation
Plasmid DNA Purification
illustra plasmidPrep Mini Spin Kit
illustra plasmidPrep Mini Spin Kit
illustra plasmidPrep Midi Flow Kit
illustra plasmidPrep Midi Flow Kit
Sephacryl™ S-1000 SF
Yeast Plasmid Isolation Kit
50
250
25
100
750 ml
50
28-9042-69
28-9042-70
28-9042-67
28-9042-68
17-0476-01
US7922050RXNS
Rolling Circle Amplification – Template Amplification
illustra TempliPhi™ 100 DNA Amplification Kit 100
illustra TempliPhi 500 DNA Amplification Kit
500
illustra TempliPhi Sequence Template
10000
Preparation Kit
illustra TempliPhi Large Construct Kit
1000
illustra TempliPhi Sequence Resolver Kit
20 rxns
illustra TempliPhi Sequence Resolver Kit
50 rxns
illustra TempliPhi Sequence Resolver Kit
200 rxns
25-6400-80
28-9035-29
28-9035-30
28-9035-31
RNA Preparation
Total RNA
illustra RNAspin Mini Kit
illustra RNAspin Mini Kit
illustra RNAspin Mini Kit
illustra RNAspin Midi Kit
illustra RNAspin 96 Kit
illustra RNAspin 96 Kit
illustra RNAspin 96 Kit
illustra RNAspin 96 Filter Plate
illustra CsTFA (solution)
20
50
250
20
2 × 96
4 × 96
24 × 96
1
100 ml
25-0500-70
25-0500-71
25-0500-72
25-0500-73
25-0500-74
25-0500-75
25-0500-76
25-0500-88
17-0847-02
mRNA
illustra QuickPrep™ Micro mRNA Purification Kit
illustra QuickPrep mRNA Purification Kit
illustra mRNA Purification Kit
illustra mRNA Purification Kit
1
4
2
4
27-9255-01
27-9254-01
27-9258-01
27-9258-02
PCR
PCR Kits & Components
illustra Hot Start Mix RTG 0.5 ml
illustra Hot Start Mix RTG 0.2 ml
illustra Hot Start Mix RTG 0.2 ml
illustra Hot Start Master Mix
illustra PuReTaq™ Ready-To-Go™ PCR Beads 0.2 ml
illustra PuReTaq Ready-To-Go PCR Beads 0.2 ml
illustra PuReTaq Ready-To-Go PCR Beads 0.5 ml
illustra PuReTaq Ready-To-Go PCR Beads 0.2 ml
FideliTaq™ PCR Master Mix (2x)
FideliTaq PCR Master Mix (Plus)
FideliTaq DNA Polymerase
FideliTaq DNA Polymerase
FideliTaq DNA Polymerase
FideliTaq DNA Polymerase
FideliTaq DNA Polymerase
Taq DNA Polymerase (cloned)
Taq DNA Polymerase (cloned)
Taq DNA Polymerase (cloned)
illustra Ready-To-Go RAPD Analysis Beads
illustra Ready-To-Go RAPD Analysis Kit
100 rxn
96 rxn
480 rxn
100
96 rxn plate
5 × 96 rxn plate
100 rxn tubes
96 rxn h-tube
100
100
50 units
250 units
1000 units
5 × 250 units
5000 units
250 units
4 × 250 units
10 × 250 units
100
100 + 6 Primers
28-9006-46
28-9006-53
28-9006-54
25-1500-01
27-9557-01
27-9557-02
27-9558-01
27-9559-01
E71182
E71183
27-0798-10
27-0798-11
27-0798-12
27-0798-13
27-0798-14
27-0799-04
27-0799-05
27-0799-06
27-9500-01
27-9502-01
25-6400-10
25-6400-50
25-6400-01
Columns
RT-PCR
RT-PCR Kits & Components
illustra Ready-To-Go RT-PCR Beads 0.5 ml
illustra Ready-To-Go RT-PCR Beads 0.2 ml
illustra Ready-To-Go RT-PCR Beads 0.2 ml
RT-PCR Master Mix (2X)
FideliTaq RT-PCR Master Mix (2X)
Pack size
100 rxn tubes
96 rxn tube
96 rxn h-tube
100
100
Code No.
27-9266-01
27-9267-01
27-9259-01
E78370
E71185
DNA Clean-up
Purification of PCR Products and Restriction Fragments
illustra GFX™ PCR DNA and Gel Band
100
Purification Kit
illustra GFX PCR DNA and Gel Band Purification Kit 250
illustra GFX 96 PCR Purification Kit
10 × 96
illustra MicroSpin™ S-400 HR columns
50
illustra MicroSpin S-300 HR columns
50
illustra MicroSpin G-25 columns
50
ExoSAP-IT™
100
ExoSAP-IT
500
ExoSAP-IT
2000
28-9034-71
28-9034-45
27-5140-01
27-5130-01
27-5325-01
US78200
US78201
US78202
Dye Terminator Removal
illustra AutoSeq™ G-50
illustra AutoSeq G-50
illustra AutoSeq G-50
illustra MicroSpin G-50 columns
illustra MicroSpin G-50 columns
illustra MicroSpin S-200 HR columns
50
250
1000
50
250
50
27-5340-01
27-5340-02
27-5340-03
27-5330-01
27-5330-02
27-5120-01
Labeling and Clean-up
illustra CyScribe™ GFX Purification Kit
illustra CyScribe GFX Purification Kit
illustra NICK™ columns
illustra NICK columns
illustra ProbeQuant™ G-50 Micro columns
25
50
20
50
50
27-9606-01
27-9606-02
17-0855-01
17-0855-02
28-9034-08
Desalting and Nucleotide Removal
illustra MicroSpin G-25 columns
illustra NAP™-5
illustra NAP-5
illustra NAP-10
illustra NAP-10
illustra NAP-25
illustra NAP-25
illustra Sephadex™ G-25 DNA Grade SF
illustra Sephadex G-50 DNA Grade F
illustra Sephadex G-50 DNA Grade F
illustra Sephadex G-100 DNA Grade SF
50
20
50
20
50
20
50
100 g
25 g
100 g
100 g
27-5325-01
17-0853-01
17-0853-02
17-0854-01
17-0854-02
17-0852-01
17-0852-02
17-0572-02
17-0573-01
17-0573-02
17-0574-02
25 µmol
100 µmol
500 µmol
25 µmol
100 µmol
500 µmol
25 µmol
100 µmol
500 µmol
25 µmol
100 µmol
500 µmol
25 µmol
100 µmol
4 × 25 µmol
4 × 100 µmol
4 × 500 µmol
10 µmol
28-4065-01
28-4065-02
28-4065-03
28-4065-11
28-4065-12
28-4065-13
28-4065-21
28-4065-22
28-4065-23
28-4065-31
28-4065-32
28-4065-33
28-4065-41
28-4065-42
28-4065-51
28-4065-52
28-4065-53
28-4065-57
40 µmol
(4 × 10 µl)
500 µl
28-4065-58
28-4065-60
1 ml
28-4065-62
500 µl
28-4065-64
50
28-9425-44
Nucleotides
illustra dATP, 100 mM
illustra dATP, 100 mM
illustra dATP, 100 mM
illustra dCTP, 100 mM
illustra dCTP, 100 mM
illustra dCTP, 100 mM
illustra dGTP, 100 mM
illustra dGTP, 100 mM
illustra dGTP, 100 mM
illustra dTTP, 100 mM
illustra dTTP, 100 mM
illustra dTTP, 100 mM
illustra dUTP, 100 mM
illustra dUTP, 100 mM
illustra dNTP Set (100 mM each A,C,G,T)
illustra dNTP Set (100 mM each A,C,G,T)
illustra dNTP Set (100 mM each A,C,G,T)
illustra DNA Polymerization Mix dNTP Set
(20 mM each A,C,G,T)
illustra DNA Polymerization Mix dNTP Set
(20 mM each A,C,G,T)
illustra PCR Nucleotide Mix dNTP Set
(25 mM each A,C,G,T)
illustra PCR Nucleotide Mix dNTP Set
(2 mM each A,C,G,T)
illustra PCR Nucleotide Mix dNTP Set
(10 mM each A,C,G,T)
DNA, RNA and protein preparation
illustra triplePrep Kit
28-9034-70
GE, imagination at work, and GE monogram are
trademarks of General Electric Company.
ÄKTA, AutoSeq, CyDye, CyScribe, genomicPrep,
GenomiPhi, GFX, GraviTrap, GSTprep, GSTrap,
HiTrap, HisTrap, HisPrep, illustra, LabMate, MBPTrap,
MicroSpin, MidiTrap, MiniTrap, MultiTrap, NanoVue, NAP,
NICK, PreScission, ProbeQuant, PuReTaq, QuickPrep,
Ready-To-Go, Sephacryl, Sephadex, Sepharose,
SpinTrap, StrepTrap, TempliPhi, and Whatman are
trademarks of GE Healthcare companies.
CyDye: This product, or portions thereof, is manufactured
under an exclusive license from Carnegie Mellon
University under US patent number 5,268,486 and
equivalent patents in the US and other countries.
ExoSAP-IT: Exonuclease I/Shrimp Alkaline Phosphatase
method of use is covered by one or more of the
following US patent numbers: 5,756,285 and 5,741,676
in the name of GE Healthcare Bio-Sciences Corp.
pGEX Vectors are to be used for scientific investigation
and research and for no other purpose whatsoever
and a license for commercial use of the licensed
products and the processes claimed in US patent
5,654,176 and equivalent patents and patent
applications in other countries must be negotiated
directly with Millipore Corp (formerly Chemicon
International Inc) by the purchaser prior to such use.
Phi 29 DNA Polymerase: Phi 29 DNA polymerase and its
use for DNA synthesis is covered by US patent numbers
5,854,033, 5,198,543, 5,576,204 and 5,001,050.
StrepTrap HP: This product is covered by US patent
number 6,103,493 and equivalent patents and patent
applications in other countries. The purchase of
StrepTrap HP includes a license under such patents for
non-profit and in-house research only. Please contact
IBA ([email protected]) for further information on
licenses for commercial use of StrepTactin.
Taq DNA Polymerase: This product is sold under
licensing arrangements with Roche Molecular Systems,
F Hoffmann-La Roche Ltd and the Perkin-Elmer
Corporation. Purchase of this product is accompanied
by a limited license to use it in the Polymerase Chain
Reaction (PCR) process for research in conjunction
with a thermal cycler whose use in the automated
performance of the PCR process is covered by the upfront license fee, either by payment to Perkin-Elmer or
as purchased (i.e., an authorized thermal cycler).
FideliTaq is a trademark of USB Corporation.
FideliTaq DNA Polymerase technology is licensed under
U.S. Patent 5,436,149 owned by Takara Bio Inc.
2-D Protein Extraction Buffers and Mammalian
Extraction Buffer and Yeast Extraction Buffer Kit are
manufactured by g-Biosciences, St Louis, MO, USA
All third party trademarks are the property of their
respective owners.
© 2007-2010 General Electric Company—All rights reserved.
First published Dec. 2007
All goods and services are sold subject to the terms and
conditions of sale of the company within GE Healthcare
which supplies them. A copy of these terms and
conditions is available on request. Contact your local
GE Healthcare representative for the most current
information.
US patent numbers 5,284,933 and 5,310,663, and
equivalent patents and patent applications in other
countires (assignee: Hoffman La Rouch, Inc) relate to
the purification and preparation of fusion proteins
and affinity peptides comprising at least two adjacent
histidine residues (commonly known as the histidinetag technology).
GE Healthcare UK Limited
Amersham Place
Little Chalfont
Buckinghamshire, HP7 9NA
UK
GE Healthcare Europe, GmbH
Munzinger Strasse 5
D-79111 Freiburg
Germany
GE Healthcare Bio-Sciences Corp.
800 Centennial Avenue, P.O. Box 1327
Piscataway, NJ 08855-1327
USA
GE Healthcare Bio-Sciences KK
Sanken Bldg., 3-25-1, Hyakunincho
Shinjuku-ku, Tokyo 169-0073
Japan
For local office contact information, visit
www.gelifesciences.com/contact
GE Healthcare Bio-Sciences AB
Björkgatan 30
751 84 Uppsala
Sweden
www.gelifesciences.com/sampleprep
imagination at work
28-9337-00 AE 06/2010