HemaSpot™: Advanced Blood Sampling Jeanette Hill Spot On Sciences, Austin, TX Introduction HemaSpot Design HemaSpot (patent pending) is an easy-to-use device for self sampling and robust storage/shipping of blood samples at ambient temperatures. HemaSpot uses a finger stick to collect and dry blood within a protective cartridge. Once dried, the sample is stable at ambient temperature and can be safely and easily shipped to a diagnostic test site for analysis. Based on dried blood spot technology and leveraging the phenomenal success of newborn blood screening, HemaSpot allows anyone to take a blood sample at any time and any location. This enables important research and diagnostic testing that was not previously feasible. Easy Remote Blood Sampling Innovative Fan-shaped Form Traditional dried blood spot circle HemaSpot fan form 100 80 2. Add 2 drops of blood (~70 µL) to device 3. Close lid and ship at ambient temperature for analysis or storage 60 % Recovery 1. Stick finger with lancet 40 20 Small Molecule Analysis by LC-MS/MS 0 319 TFN CF10 903 Filter Paper Grades DNA (total) Faster drying than circle Protein (Hb) Small Molecule (Oxycodone) Efficient Analyte Recovery Summary •HemaSpot allows self-sampling and immediate transport at ambient temperature. •Samples can be stably stored in HemaSpot for years at room temperature. •All major analytes including nucleotides, proteins and small molecules can be efficiently extracted and analyzed by LC-MS/MS, RT-PCR, ELISA, etc. •The fan form increases drying rate and improves sample consistency. 45 amino acids analyzed in a single injection from one HemaSpot blade. Comparison of whole blood and dried blood samples. AB Sciex aTRAQ method Mean of 5 separate donors HemaSpot enables remote blood collection by allowing a sample to be taken by anyone at any time and anywhere. This material is based upon work supported by DARPA under Contract No. W31P4Q-11-C-0234. www.spotonsciences.com [email protected] 512-827-9627