: SEAL “Technical Tips” Sample Addition “Sample Addition Mode” has been incorporated into AQ1 Software, allowing the user to stop the run in progress, add samples, and restart the run. The included example demonstrates the entire process, from the set up of the original schedule to the completion of the restarted run to which samples have been added. Below is the original “Scheduling” screen where the user first schedules the tray: SEAL Analytical - Mequon Technology Center 10520-C Baehr Road Mequon, WI 53092 262-241-7900 www.Seal-us.com Copyright © 2010 SEAL Analytical Reproduction or distribution of this document is expressly forbidden without written permission from SEAL Analytical 29 : SEAL “Technical Tips” Sample Addition After the tray has been saved in ”Scheduling”, the run is initiated by doubleclicking the „Run‟ icon. The “Tray Selection” screen then appears where the desired tray is selected for analysis. The “Options” screen then displays tick boxes where the user specifies whether they want to auto standardize, perform water baselines, and/or reset the reaction segment counter. SEAL Analytical - Mequon Technology Center 10520-C Baehr Road Mequon, WI 53092 262-241-7900 www.Seal-us.com Copyright © 2010 SEAL Analytical Reproduction or distribution of this document is expressly forbidden without written permission from SEAL Analytical 30 : SEAL “Technical Tips” Sample Addition If the „Auto Standardization‟ tick box is selected from the previous screen, the “Auto Standard Preparation” screen appears. This screen allows the user to confirm where the blank and top standard are located. Once the user has verified that all the information is correct, clicking „OK‟ will start the run. SEAL Analytical - Mequon Technology Center 10520-C Baehr Road Mequon, WI 53092 262-241-7900 www.Seal-us.com Copyright © 2010 SEAL Analytical Reproduction or distribution of this document is expressly forbidden without written permission from SEAL Analytical 31 : SEAL “Technical Tips” Sample Addition The “Run” screen will now appear. The „Sample Addition‟ icon, located at the top of the screen, will initiate “Sample Addition Mode.” Sample Addition icon SEAL Analytical - Mequon Technology Center 10520-C Baehr Road Mequon, WI 53092 262-241-7900 www.Seal-us.com Copyright © 2010 SEAL Analytical Reproduction or distribution of this document is expressly forbidden without written permission from SEAL Analytical 32 : SEAL “Technical Tips” Sample Addition Once the user clicks the „Sample Addition‟ icon, the screen shown below appears allowing the user to choose between the following two options: “Stop at the end of the current cycle” or “Finish all work in progress.” If “Stop at the end of the current cycle” is selected, the AQ1 will finish the current cycle, immediately go through its wash cycle, and stop. The samples and/or standards that were prepared, but not aspirated, will remain in the reaction wells and will be reprepared after restarting the run. If “Finish all work in progress” is chosen, the AQ1 will continue to aspirate and read all samples and/or standards that have been prepared and are incubating. The AQ1 will then go through its wash cycle and stop. SEAL Analytical - Mequon Technology Center 10520-C Baehr Road Mequon, WI 53092 262-241-7900 www.Seal-us.com Copyright © 2010 SEAL Analytical Reproduction or distribution of this document is expressly forbidden without written permission from SEAL Analytical 33 : SEAL “Technical Tips” Sample Addition After clicking „Continue‟, the “Scheduling” screen appears which allows the user to add samples. Both options (i.e. “Finish work in progress” and “Stop at the end of current cycle”) allow samples to be added to the schedule while the AQ1 is stopping. SEAL Analytical - Mequon Technology Center 10520-C Baehr Road Mequon, WI 53092 262-241-7900 www.Seal-us.com Copyright © 2010 SEAL Analytical Reproduction or distribution of this document is expressly forbidden without written permission from SEAL Analytical 34 : SEAL “Technical Tips” Sample Addition Notes: 1) New samples can only be added using this feature. AQ1 software does not allow the user to edit or add tests to previously scheduled samples! 2) If running multiple tests on samples and if scheduling multiple tests for the samples that are added on, please note: If ”Sample Addition Mode” is initiated while the AQ1 is still performing Test 1 and has not started Test 2, the AQ1 will complete the work in the following order: a) Remaining „PND‟ samples for Test 1 b) Samples added on for Test 1 c) All samples for Test 2 If ”Sample Addition Mode” is initiated while the AQ1 is performing Test 2, after having completed Test 1, the AQ1 will complete the work in the following order: a) Samples added on for Test 1 b) Remaining „PND‟ samples for Test 2 c) Samples added on for Test 2 3) Neither auto-calibrants nor manual calibrants can be added to the schedule in “Sample Addition Mode”. 4) If the frequency counting feature of QC Pro is being used for a test, the QC Pro settings in Test Parameters must have „1‟ located in the „Start Before‟ field. This will ensure the QC is inserted prior to the first added sample. SEAL Analytical - Mequon Technology Center 10520-C Baehr Road Mequon, WI 53092 262-241-7900 www.Seal-us.com Copyright © 2010 SEAL Analytical Reproduction or distribution of this document is expressly forbidden without written permission from SEAL Analytical 35 : SEAL “Technical Tips” Sample Addition The “Scheduling” screen below shows the samples that have been added in cups 6 – 9. SEAL Analytical - Mequon Technology Center 10520-C Baehr Road Mequon, WI 53092 262-241-7900 www.Seal-us.com Copyright © 2010 SEAL Analytical Reproduction or distribution of this document is expressly forbidden without written permission from SEAL Analytical 36 : SEAL “Technical Tips” Sample Addition After the addition of necessary samples, click on the „Run Screen‟ icon, located on the left side on the screen, to view the status of the initial run. Once the status indicator bar displays “Finished”, click the „Restart Run‟ icon to initiate the run with the added samples. SEAL Analytical - Mequon Technology Center 10520-C Baehr Road Mequon, WI 53092 262-241-7900 www.Seal-us.com Copyright © 2010 SEAL Analytical Reproduction or distribution of this document is expressly forbidden without written permission from SEAL Analytical 37 : SEAL “Technical Tips” Sample Addition After clicking the „Restart Run‟ icon, the “Options” screen appears where the user has the option to reset the reaction segment counter and/or perform water baselines. Please note, although it is possible to perform water baselines at this point, it is not recommended. SEAL Analytical - Mequon Technology Center 10520-C Baehr Road Mequon, WI 53092 262-241-7900 www.Seal-us.com Copyright © 2010 SEAL Analytical Reproduction or distribution of this document is expressly forbidden without written permission from SEAL Analytical 38 : SEAL “Technical Tips” Sample Addition Once the user has clicked „OK‟, the “Run” screen will appear with the additional samples appended to the tray. Below is the updated “Run” screen which includes the added samples as well as the proper insertion of QC‟s. SEAL Analytical - Mequon Technology Center 10520-C Baehr Road Mequon, WI 53092 262-241-7900 www.Seal-us.com Copyright © 2010 SEAL Analytical Reproduction or distribution of this document is expressly forbidden without written permission from SEAL Analytical 39 : SEAL “Technical Tips” Sample Addition Once the status indicator bar displays “Finished”, the run is complete. SEAL Analytical - Mequon Technology Center 10520-C Baehr Road Mequon, WI 53092 262-241-7900 www.Seal-us.com Copyright © 2010 SEAL Analytical Reproduction or distribution of this document is expressly forbidden without written permission from SEAL Analytical 40
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