S A M P L E P R E P A R A T I O N Chrom Tech Filtration Centrifuge Filter Tubes Micro, Midi, and Maxi Spin centrifuge filters are very useful for small volume sample filtration. Designed for use in centrifuges, the filter units consist of a sample chamber and receiver tube. This tube has a marking area and is graduated. A cap serves the dual purpose of capping the sample chamber during the centrifugation and the receiver tube after the sample chamber has been removed. Each package is imprinted to identify the pore size and membrane type. Micro-Spin Filter Tubes Quickly and easily clarify small sample volumes with Chrom Tech's Micro-Spin® centrifuge filter tubes. These devices fit in any standard microcentrifuge, and allow you to process multiple samples simultaneously. Less than 5µL hold-up volume provides near quantitative volumetric recovery. Maximum G-force: 10,000xG. Micro-Spin centrifuge filter tubes now include glass prefilters. FE AT U RE S   For up to 850 µL sample volumes Polypropylene housings Midi-Spin Filter Tubes Midi-Spin® filter tubes consist of a polypropylene insert that houses a filter membrane and a tapered 5.0ml filtrate tube. Inserts can accommodate sample volumes up to 2ml. Maximum G-force: 5,000xG. FE AT U RE S   For up to 5 mL sample volumes Polypropylene housings Maxi-Spin Filter Tubes Maxi-Spin® centrifuge filter tubes remove particulates from larger sample sizes with any centrifuge that holds 50 mL conical tubes. They should only be used with a fixed-angle rotor centrifuge to prevent premature clogging of the membrane. Maximum operating temperature: 50º C. Maximum G-force: 2,500xG. FE AT U RE S    For up to 25 mL sample volumes Polypropylene housings Used with fixed angle rotor centrifuge © 2 0 1 4 C h rom Te c h , In c . P rod u c t n a m e s a re tra d e m a r ks o r reg ist ered t r a d e m a rks of the i r re sp e c ti ve co mpanies Pub . No . : 8 9 0 0 0 distributed by [ 800.822.5242 | (MN) 952.431.6000 [email protected] | www.chromtech.com ] Chrom Tech Filtration S A M P L E P R E P A R A T I O N Centrifuge Filter Tubes (continued) M E M BR A N E PO R E SIZ E MICRO 100/pk MID I 25/pk MAXI 50/pk Cellulose Acetate 0.2 µm CTF-CA020-01 CTF-CA020-02 CTF-CA020-03 0.45 µm CTF-CA045-01 CTF-CA045-02 CTF-CA045-03 0.2 µm CTF-NC020-01 CTF-NC020-02 CTF-NC020-03 0.45 µm CTF-NC045-01 CTF-NC045-02 CTF-NC045-03 0.2 µm CTF-NY020-01 CTF-NY020-02 CTF-NY020-03 0.45 µm CTF-NY045-01 CTF-NY045-02 CTF-NY045-03 0.2 µm CTF-PS020-01 CTF-PS020-02 CTF-PS020-03 0.45 µm CTF-PS045-01 CTF-PS045-02 CTF-PS045-03 0.2 µm CTF-PP020-01 CTF-PP020-02 CTF-PP020-03 0.45 µm CTF-PP045-01 CTF-PP045-02 CTF-PP045-03 0.2 µm CTF-PT020-01 CTF-PT020-02 CTF-PT020-03 0.45 µm CTF-PT045-01 CTF-PT045-02 CTF-PT045-03 0.2 µm CTF-PV020-01 CTF-PV020-02 CTF-PV020-03 0.45 µm CTF-PV045-01 CTF-PV045-02 CTF-PV045-03 0.2 µm CTF-RC020-01 CTF-RC020-02 CTF-RC020-03 0.45 µm CTF-RC045-01 CTF-RC045-02 CTF-RC045-03 NC (Nitrocellose) Nylon Polyethersulfone Polypropylene PTFE (polytetrafluoroethylene) PVDF (polyvinylidene diffuoride) Regenerated Cellulose Membrane Selection Guide RC Nylon Regenerated Cellulose (RC) is a hydrophilic, solvent resistant, low protein binding membrane. Ideal for removing particulates from HPLC samples prior to injection. Compatible with all HPLC solvents. Use for particle removal and degassing of solvents. RC membranes are also compatible with aqueous solutions in the 3 to 12 pH range. Extractables with water are less than 1%. RC membranes exhibit low, non-specific adsorption — good for biological sample filtration where maximum protein recovery is important. When used with a glass prefilter, RC is ideal for tissue culture media filtration, as well as general biological sample filtration. Commonly used for general laboratory filtration and filtration of HPLC samples prior to injection. Nylon is solvent resistant and exhibits low extractables. Nylon binds protein and should not be used when protein recovery is important. PTFE PTFE (polytetrafluoroethylene) is a hydrophobic and chemically resistant to all solvents, acids and bases. Does not impart extractables to the filtrate. PTFE blocks water vapor, making it ideal for transducer protectors. Also ideal for filtering and degassing chromatography solvents. PVDF PVDF (polyvinylidene diflouride) is a hydrophilic, solvent resistant membrane that exhibits low levels of UV adsorbing extractables. Use for HPLC sample filtration and general biological filtration. PVDF is a low protein binding membrane. Polypropylene Polypropylene is a hydrophilic membrane that exhibits a wide range of chemical compatibility to organic solvents. A good choice for HPLC sample filtration when performing chromatography protein analysis. High solvent resistant. Low non-specific adsorbing membrane for maximum protein recovery in critical analysis. Also well suited for biological sample filtration. Glass Microfiber Glass Microfiber membranes are commonly used as pre-filters to remove large particulates and extend the load capacity of the membrane. Cellulose Acetate Cellulose Acetate is a very low protein binding membrane, ideal for aqueous based samples. CA membranes are an excellent choice when maximum protein recovery in filtrate is critical. Laboratory studies show the CA membranes bind less protein that PVDF. When used with a glass pre-filter, CA is ideal for tissue culture media filtration and sensitive biological samples. The pre-filter increases yield. NC NC (Nitrocellulose - Mixed Esters (ME) - unsupported filters are hydrophilic and composed of a mixture of inert cellulose nitrate and cellulose acetate polymers. The uniform microporous structure of these filters provides the fastest flow rates and highest throughputs available in a membrane filter. Because they are biologically inert, NC filters are ideal for a wide range of clarification, sterilization, and analytical applications. PS PS (Polyethersulfone) is the filter of choice for tissue culture work. It has very low extractables. Low binding of protein and nucleic acids. It has relatively broad chemical resistance except to aggressive solvents and it has good to excellent flow rates. distributed by [ 800.822.5242 | (MN) 952.431.6000 [email protected] | www.chromtech.com ] S A M P L E P R E P A R A T I O N Chrom Tech Filtration Forensic Spin Tubes Our kit includes a 800 µL filter basket and a 2.0 mL microcentrifuge receiver tube with attached cap. The 1.4 mm mesh acts as a sieve to retain cloth samples and pass fluids to the receiver tube. FE AT U RE S    800 µL capacity Extracts fluids from cloth samples Polypropylene housing DE SCRI P T I O N QTY PART N O Forensic Spin Tube 100 CTF-FS000-01 Membrane Filters We offer a wide variety of precut membranes in Nylon, Cellulose Acetate, PVCF, PS, Nitrocellose and PTFE to suit your application needs. All are available in sizes ranging from 13 mm to 142 mm. M E M BR A N E PORE SIZE 13 mm 100/pk 2 5 mm 100/pk 47 mm 100/pk 90 mm 25/pk 142 m m 25/pk Cellulose Acetate 0.2 µm CTF-CA020-13 CTF-CA020-25 CTF-CA020-47 CTF-CA020-90 CTF-CA020-142 0.45 µm CTF-CA045-13 CTF-CA045-25 CTF-CA045-47 CTF-CA045-90 CTF-CA045-142 Glass Fiber 0.7 µm CTF-GF070-13 CTF-GF070-25 CTF-GF070-47 CTF-GF070-90 N/A 1.0 µm CTF-GF100-13 CTF-GF100-25 CTF-GF100-47 CTF-GF100-90 N/A 3.1 µm CTF-GF310-13 CTF-GF310-25 CTF-GF310-47 CTF-GF310-90 N/A NC Nylon PS PTFE PVDF 5.0 µm CTF-GF500-13 CTF-GF500-25 CTF-GF500-47 CTF-GF500-90 N/A 0.2 µm CTF-NC020-13 CTF-NC020-25 CTF-NC020-47 CTF-NC020-90 CTF-NC020-142 0.45 µm CTF-NC045-13 CTF-NC045-25 CTF-NC045-47 CTF-NC045-90 CTF-NC045-142 0.2 µm CTF-NY020-13 CTF-NY020-25 CTF-NY020-47 CTF-NY020-90 CTF-NY020-142 0.45 µm CTF-NY045-13 CTF-NY045-25 CTF-NY045-47 CTF-NY045-90 CTF-NY045-142 0.2 µm CTF-PS020-13 CTF-PS020-25 CTF-PS020-47 CTF-PS020-90 CTF-PS020-142 0.45 µm CTF-PS045-13 CTF-PS045-25 CTF-PS045-47 CTF-PS045-90 CTF-PS045-142 0.2 µm CTF-PT020-13 CTF-PT020-25 CTF-PT020-47 CTF-PT020-90 CTF-PT020-142 0.45 µm CTF-PT045-13 CTF-PT045-25 CTF-PT045-47 CTF-PT045-90 CTF-PT045-142 0.2 µm CTF-PV020-13 CTF-PV020-25 CTF-PV020-47 CTF-PV020-90 CTF-PV020-142 0.45 µm CTF-PV045-13 CTF-PV045-25 CTF-PV045-47 CTF-PV045-90 CTF-PV045-142 distributed by [ 800.822.5242 | (MN) 952.431.6000 [email protected] | www.chromtech.com ]