Principles of Genetic Analysis MGY315H Laboratory Manual 2015 University of Toronto Molecular Genetics and Microbiology Program (MGY) Course coordinator and Drosophila section: Dr. Julie Brill The Hospital for Sick Children PGCRL, 686 Bay Street 15th floor, Rm. 15.9716 416-813-8863 [email protected] Yeast section: Dr. Brigitte Lavoie Medical Sciences Building 1 King’s College Circle Rm. 4278 416-978-6123 [email protected] TAs: Lucy Xie <[email protected]> Lauren Del Bel <[email protected]> Fly TA: Jonathan (Jon) Ma <[email protected]> Late policy: 10% to be deducted per day up to 2 days late. Reports more than 2 days late will receive a mark of zero. Textbook: A.J.F. Griffiths, S.R. Wessler, S.B. Carroll and J. Doebley (2012) Introduction to Genetic Analysis, Tenth Edition (New York: W.H. Freeman and Company). MGY315 Winter 2014 Yeast Genetics Section Experiments Schedule Yeast Lab 1: (Jan. 9, 2014) 1. START Yeast Experiment 1 – Genetic Analysis of Mating: replica-plate onto various mating and pheromone testing plates 2. START Yeast Experiment 2 – SGA: set up mating plates Yeast Lab 2: (Jan. 16, 2014) 1. CONTINUE Yeast Experiment 2 – SGA: replica pin onto SPO media 2. START Yeast Experiment 3 – Elucidation of a Genetic Pathway: replica-plate onto various SD-HIS media 3. FINISH Yeast Experiment 1 – Genetic Analysis of Mating: score plates Yeast Lab 3: (Jan. 23, 2014) 1. CONTINUE Yeast Experiment 2 – SGA: replica pin onto MATa selection media 2. FINISH Yeast Experiment 3 – Elucidation of a Genetic Pathway: score plates Yeast Lab 4: (Jan. 30, 2014) 1. CONTINUE Yeast Experiment 2 – SGA: replica pin onto MATa+G418+NAT selection media 2. START/FINISH Yeast Experiment 4 – Meiotic Segregation Analysis: score tetrad plates 3. START Yeast Experiment 5 – Haploid Invasive Growth: spread plate and mutagenize Σ1278b haploid strain; streak Σ1278b diploid strains onto SD-HIS to obtain MATa/MATa diploids Yeast Lab 5: (Feb. 6, 2014) 1. CONTINUE Yeast Experiment 2 – SGA: score final selection plates 2. FINISH Yeast Experiment 5 – Haploid Invasive Growth: score mutagenized plates and tetrads Yeast Lab 6: (Feb. 13, 2014) 1. FINISH Yeast Experiment 2 – SGA: hand in lab write-up at end of class MGY315 Winter 2014 Drosophila Genetics Section Experiments Schedule Fly Lab I (Feb. 27, 2014) Experiment 1, Part 1: Examining wild-type Drosophila melanogaster Experiment 3, Part 1: Setting up Recombination Mapping Cross I Experiment 4, Part 1: Setting up Unknown Mutant Cross 1 LAB REPORT I: Due at the end of class (2 marks) Fly Lab II (Mar. 6, 2014) Experiment 4, Part 2: Turning Unknown Mutant Cross 1 Experiment 2: Polytene Chromosome Lab LAB REPORT II: Formal Lab Report Due on Mar. 14 (4 marks) ****Collecting flies for Unknown Mutant crosses (Mar. 11-14) Experiment 4, Part 3: Collecting Unknown Mutant Progeny [TUES-FRI] Fly Lab III (Mar. 13, 2014) Experiment 1, Part 2: Examining single mutant flies Experiment 3, Part 2a: Setting up Recombination Mapping Cross 2 Experiment 3, Part 2b: Examining multiple mutant flies and F1 progeny LAB REPORT III: Due at the end of class GENETICS PRINCIPLES WORKSHEET: Due on Mar. 20 (2 marks) (5 marks) ****Collecting flies for Unknown Mutant crosses (Mar. 17-20) Experiment 4, Part 4a: Collecting Unknown Mutant Progeny [MON-THURS] Fly Lab IV (Mar. 20, 2014) Experiment 4, Part 4b: Setting up Unknown Mutant Cross 2 Experiment 4, Part 4c: Examining Unknown Mutant flies and F1 Progeny Fly Lab V (Mar. 27, 2014) Experiment 3, Part 3: Scoring F2 Progeny from Recombination Mapping Crosses LAB REPORT V: Formal Lab Report Due on Apr. 3 (6 marks) Fly Lab VI (Apr. 3, 2014) Experiment 4, Part 5: Scoring F2 Progeny from Unknown Mutant Crosses WORKSHEET: Answers will be posted on Blackboard by TAs LAB REPORT VI: Formal Lab Report Due on Apr. 3* (6 marks) *Note that you may take up to one extra week to finish your report (turn in the finished report to your TA) Final Exam (date to be determined) Eukaryotic Genetics Section (Yeast and Flies – 40% of total mark)