490 Series
Biological Microscope
OPERATOR’S MANUAL
READ THIS MANUAL BEFORE USING THE MICROSCOPE
Contents
490 SERIES
Before use .............................................................................................. 3
1. Nomenclature .................................................................................... 4
2. Operation ............................................................................................ 8
2-1 Assembly ........................................................................... 8
2-2 Setting up .......................................................................... 9
2-3 Focusing ............................................................................. 9
2-4 Adjusting the condenser and diaphragm ............. 10
2-5 Using the trinocular port ............................................. 11
2-6 Setting the stage’s stop limit ...................................... 11
2-7 Adjusting focusing tension .........................................12
2-8 Changing the bulb ......................................................... 12
2-9 Changing the fuse ..........................................................12
2-10 Maintenance ....................................................................13
3. General specifications .................................................................... 14
4. Configuration drawing ...................................................................15
5. Optional Accessories .......................................................................16
6. Parameters/technical terms ......................................................... 17
6-1 Objectives ..........................................................................17
6-2 Eyepieces ...........................................................................17
6-3 Electrical system ..............................................................18
6-4 Parameters ........................................................................ 18
6-5 Technical Terms ............................................................... 18
7. Troubleshooting ............................................................................... 20
2
Before use
490 SERIES
Introduction
Congratulations on the purchase of your new AmScope Microscope!
This manual is designed for the B490 and T490 series microscopes.
Though both have identical bases, the B490 is equipped with a
binocular head (2 eyetubes) and the T490 is equipped with a trinocular
head (2 eyetubes & 1 photo/video port). Please take a few minutes to
familiarize yourself with the features and functions of your new
microscope. If you want more information on microscope, parts, and
accessories, please visit our website at:
www.AmScope.com
We recommend that you study this manual thoroughly before operating the microscope, and that you keep it on hand for future reference.
If you have additional questions or need assistance, please send us an
email at:
[email protected]
Please include the microscope model number in your email so that we
can identify your model and provide immediate help.
Precautions
(1) As the microscope is a precision instument, always handle it with
care, avoiding impact or abrupt movement during transportation. Do
not shake the package.
(2) Do not place the microscope in direct sunlight or in high heat. Keep
it indoors in a dry and clean place with temperatures between 32°-100°
F (0°-40°C), maximum relative humidity: 85%
(3) Avoid touching the lenses on the objective and the eyepiece so that
oil and dirt from your fingerprints do not obstruct your view.
(4) Before turning the power on, make sure that the power supply
voltage is consistent with the voltage of your microscope
3
1. Nomenclature
490 SERIES
Eyepiece
Binocular
Gemel head
Diopter
Eyetube base
Nosepiece
Head -lock screw
Objective
Arm
Mechanical Stage
Tension knob
Coarse
focusing knob
Iris adjustment slider
Condenser,
iris diaphragm
Filter holder
Fine
focusing knob
Base lens
Slide controls
Base
Dimmer
Power switch
Fig. 1: B490
4
1. Nomenclature
490 SERIES
C-mount
Adjustable
trinocular port
Trinocular
Gemel head
Limit-stop
knob
Coarse
focusing knob
Fine
focusing knob
Condenser
adjustment knob
Condenser
screw
Fig. 2: T490
5
1. Nomenclature
490 SERIES
Light screw
Fuse
Halogen
bulb
Fig. 3: Bottom
Condenser
screw
Condenser
Iris adjustment
slider
Filter holder
Iris
Fig. 4: Condenser, iris and filter holder
Definitions:
Adjustable Trinocular Port
Base Lens
Coarse Focusing Knob
Threaded port with adjustable length for
attachment of digital or video camera
Directs the light source towards the slide
Used to initially bring the slide into sight and
focus
6
1. Nomenclature
490 SERIES
Condenser & Iris Diaphragm
Controls the amount of light that hits the slide
Condenser Adjustment Knob
Moves the condenser up and down
Condenser Screw
Screws the condenser in place, secureing it
to the mechanical stage
Dimmer
Controls the amount of light that escapes
from the base lens
Diopter
Allows the focus to be perfected for both eyes,
independent of each other
Fine Focusing Knob
Used to refine focus to a clear image
Head Lock Screw
Screws the microscope head into place
Limit-Stop Knob
Limits the upward movement of the mechanical
stage in order to avoid damaging the slide and
objective (adjustable).
Mechanical Stage
Mechanically moves the slide along an X and Y
axis for optimal positioning
Nosepiece
Houses the objective lenses
Rotating Gemel Head Adjusts eyepieces to fit the distance between
the user’s eyes for added comfort
Tension Knob
Adjusts the tension of the focusing knobs
7
2. Operation
490 SERIES
2-1 Assembly
(1) First take the styrofoam container out of the cardboard carton and lay
it on its side, paying attention to which side is labeled up. Remove tape
and open the container carefully so as to avoid dropping and damaging
the optical items. Check carefully to ensure that all parts and accessories
are intact.
(2) Check the parking list below:
One Microscope Body
One Binocular (B490) or Trinocular (T490) Rotating Gemel Head
One Adjustable Trinocular Photo Port (for T490 only)
Four Objectives: 4X, 10X, 40X, and 100X
Two Pairs of Eyepieces: W10X and P16X
Color Filters
One Bottle of Immersion Oil
One 6V/20W Halogen Spare Bulb
One Spare Fuse
One Dust Cover
(3) Remove the microscope body from the box and remove the plastic
protective covering. The body of the microscope is composed of the
base, the stage, the arm, and the nosepiece.
(4) Loosen the metal knob located directly above the nosepiece (the
head-lock screw) completely. Place the head, flat circular side down, onto
the circular opening at the top of the arm. Make sure the microscope
head is securely on top of the arm and tighten the head-lock screw to
lock the head in place.
(5) For the T490 model only, screw the photo port onto the top of the
microscope head.
(6) Remove the eyetube caps and drop the desired eyepieces into the
eyetubes without touching the lens.
8
2. Operation
490 SERIES
(7) Screw the objectives into the microscope nosepiece from the lowest
magnification to the highest, being careful not to touch the lens.
(8) Plug in the microscope and turn it on. If no light emerges from the
light source, adjust the dimmer knob on the side of the base.
2-2 Setting up
(1) Loosen the head-lock screw and adjust the microscope head so that it
is in a comfortable position for observation. Lock the head-lock screw.
With both eyes open, look into the eyepieces. Adjust the interpupillary
distance by holding the eyetubes and rotating the eyepieces either
towards or away from one another, until one circle of light can be seen.
(2) Place the specimen to be studied on a glass slide (or simply use a
prepared slide), and place it on the stage, holding it sungly in place with
the metal slide-holders of the mechanical stage. Using the slide controls,
center the specimen over the stage opening, lining it up with the light
and objective lens.
(3) To adjust the illumination, slowly turn the dimmer until the desired
intensity of light is achieved.
2-3 Focusing
(1) Turn the nosepiece to choose an objective. It is easiest to use the
lowest magnification first (usually 4X) to locate and focus the specimen.
As you move up in magnification, you may have to refocus the image a
little bit each time. When using the 100X objective, a little immersion oil
must be put between the objective and the cover glass on the slide in
order to minimize the distortion cause by air. Always wipe the oil off the
objective with either a glass-cleaning paper or cloth after each use.
9
2. Operation
490 SERIES
(2) Begin focusing by first looking with one eye through the eyetube
without the diopter. Close your other eye. Loosen the limit-stop knob
(located on the inside of the coarse-focusing knob) in order to give the
mechanicalstage a full range of movement and adjust the coarsefocusing knob to bring the slide into focus. You may also need to
re-center the specimen on the stage.
(3) When the object is in your field of vision, adjust the fine-focusing
knob until you see a clear, sharp image. BE ALERT: When moving the
mechanical stage vertically, be careful not to hit the objective lens with
the slide. Remember to use the limit-stop knob to avoid impact between
the objective and the slide when using the 40X and 100X objectives.
Lock the limit-stop knob at the position where the slide almost touches
the objective.
(4) Once the image is focused through the one eyepiece, look through
the other eyepiece and turn the diopter until the image is clear through
that eyepiece as well
2-4 Adjusting the condenser and diaphragm
(1) Using the condenser-adjustment knob, you can change the distance
between the light condenser and the stage. This allows you to control
the concentration of the light hitting your slide.
(2) By Changing the aperture of the iris diaphragm, you can adjust the
background brightness. Adjust the aperture of the iris diaphragmm
using the iris adjustment slider located directly under the stage.
(3) If you want to use a color filter, swivel the filter holder out, towards
you. You can now place the desired color filter into the circular opening.
Slide the filter holder back to original position before observation.
10
2. Operation
490 SERIES
2-5 Using the trinocular port
The AmScope T490 is uniquely designed so that you can focus the
image through the eyepieces and the trinocular port simultaneously.
This “parfocal” feature allows the images through the microscope’s
eyepieces, and those displayed on your computer screen or TV to be
viewed at the same time! You do not need an adapter to attach your
AmScope camera to the trinocular port. However, you may need an
adapter if you have a non-AmScope camera.
Attaching a camera:
(1) If your camera has a C-mount, simply screw the camera onto the
trinocular port.
(2) If your camera has a 23mm mounting size, remove the C-mount from
the top of the trinocular port by loosening the screw. Then, drop the
camera into the trinocular tube.
(3) To focus through the trinocular port, simply turn the middle portion
of the tube.
2-6 Setting the stage’s stop-limit
(1) Set the stage to the maximum height desired.
(2) Pull the stop-limit knob towards the stage. The stage’s stop-limit is
now set, allowing you to move the stage down, but not up past this
point.
(3) To reset the stage’s stop-limit, simply push the stop-limit knob back
towards the back panel of the microscope. The stage’s full range of
motion is now restored.
11
2. Operation
490 SERIES
2-7 Adjusting focusing tension
(1) To adjust the tension of the focusing knobs, first locate the black
ridged tension-knob on the inside of the coarse focusing knob.
(2) To decrease tension, rotate the tension adjustment forward, towards
the stage. To increase tension, rotate the tension adjustment backwards,
towards the back panel of the microscope.
2-8 Changing the bulb
(1) Before changing the light bulb, first pull the plug out of the electrical
socket and wait for the lamp to cool down in order to avoid being
burned. Also, make sure to remove the eyepieces or secure them to the
microscope before turning the microscope over!
(2) Loosen the door-screw on the bottom of the microscope and pull
open the small door. Pull the old bulb out and replace it with a new one.
Close the door and tighten the screw.
2-9 Changing the fuse
(1) First, remove the eyepieces or secure them to the microscope before
turning the microscope over.
(2) Loosen the door-screw on the bottom of the microscope and pull
opUsing a flat-head screwdriver, unscrew the fuse located on the
bottom of the microscope.
(3)Remove the old fuse and replace with a new one. Screw the fuse back
in place.
12
2. Operation
490 SERIES
2-10 Maintenance
(+) All glass surfaces must always be kept clean. Fine dust on the
optical surface should be blown off using a hand blower or gently
wiped off with a soft lens tissue. Carefully wipe off oil or fingerprints on
lens surfaces using tissue moistened with a small amount of 3:7 alcohol
to ether mixture
(+) Do not use this solution to wipe the surface of other components.
These parts, especially those made of plastic, should be cleaned with a
neutral detergent.
(+) Do not assemble or disassemble the microscope’s electrical components yourself.
(+) After use, cover the microscope with the provided dustcover. Make
sure to keep it in a dry and clean place in order to prevent rust.
13
3. Specifications
490 SERIES
Specifications for the 490 Series Biological Microscope
PARTS
WF
Eyepiece
Plan
Eyepiece
DIN
Achromatic
Objectives
Plan
Objectives
45° Viewing
Head
30° Viewing
Head
Trinocular
Port
Focusing
Nosepiece
Stage
Condenser
Diaphragm
Illumination
Lamp
Filter
SPECIFICATIONS
WF10X/18mm
WF10X/18mm with pointer
WF10X/18mm with reticle
WF16X/13mm
WF20X/10mm
P5X
P10X
P16X
4X/0.10
10X/0.25
40X(spring)/0.65
60X(spring)/0.85
100X(spring, oil)/1.25
4X
10X
40X(spring)
100X(spring, oil)
Binocular sliding head, 360° swiveling
Trinocular sliding head, 360° swiveling
Binocular Gemel head, 360° swiveling
Trinocular Gemel head, 360° swiveling
C-mount adjustable photo port.
23mm adjustable photo port.
Simultaneous Viewing
Coaxial focusing system
Quadruple nosepiece
130mm x 140mm mechanical stage
Moving range: 50mm x 76mm
Abbe, NA=1.25
Iris diaphragm
Halogen light with dimmer
ф50mm mirror
6V/20W
Blue/yellow/green
14
B490
T490
400MS
400MH45
490BH30G
15
400BL
400MR
400CD1.25
DRY100 OIL100
PA100X PA60X PA40X PA10X PA4X
A100X A40X A10X A4X
400BH45
EP5 EP10 EP16
WF15X16 WF20X10
WF10X18 WF10X18P
400TH45
490TH30G
400NP4
490 Series Biological Microscope
Configuration drawing
400FB
400PH
CAMERA
VIDEO
CCD
4. Configuration drawing
490 SERIES
5. Optional Accessories
490 SERIES
Optional Accessories
There is a variety of accessories available that can enhance your
microscope’s capabilities and give it additional features. Some of them
are listed in the table below:
NAME
Eyepiece
Objective
Darkfield
Condenser
DESCRIPTION
5X
EP5X23
20X
EP20X23
25X
EP25X23
10X with pointer
10X with reticle
2X
EP10X23P
EP10X23R
A2X
5X
A5X
20X
A20X
60X
A60X
Plan 4X
Plan 10X
Plan 40X
Plan 100X
Dry Darkfield
Condenser
Oil Darkfield
Condenser
PA4X
PA10X
PA40X
PA100X
DK-DRY100
DK-OIL100
CCD Digital
MD400 (350K)
MD600 (1.3MP)
MD700 (2.0MP)
MD800 (3.0MP)
MD900 (5.0MP)
MD1000
CCD TV Video
CCD-NP
CMOS Digital
Camera
MODEL #
16
PURPOSES
For obtai ning 20X, 50X, 200X
and 500X magnification
powers
For obtaining 80X, 200X, 800X
and 2000X magnification
powers
For obtaining 250X and 2500X
magnification powers
For easier identifying of objects
For measuring objects
For obtaining 20X and 32X
magnification powers
For obtaining 50X and 80X
magnification powers
For obtaining 200X and 320X
magnification powers
For obtaining 600X and 960X
magnification powers
For obtaining high quality high
resolution images
For obtaining low power
darkfield images
For obtaining high power
darkfield images
For obtaining still or live digital
images
For obtaining high quality still
or live digital images
For live images on TV
6. Parameters/Technical terms
490 SERIES
Optional Accessories cont’d...
Case
Phase
Contrast Kit
Aluminum case
Hard wood case
Simple
Turret
AC-400
WC-400
PCS
PCT
Stage Warmer
TCS-100
Fluorescent
Kit
FK-YX
For carrying microscope around
For storage
Phase contrast images
High quality phase contrast
images
For controlling stage
temperature
Fluorescent images
Please find more accessories on our website at: www.AmScope.com
6-1 Objectives
Type
4X
10X
40X
60X
Numerical
Aperture
(N.A.)
0.10
0.25
0.65
0.85
100X
1.25
4X
10X
40X
0.10
0.25
0.65
100X
1.25
Magnification
DIN
Achromatic
Objective
(195mm)
Plan
Objective
(195mm)
Medium
Air
Air
Air
Air
Cedar
oil
Air
Air
Air
Cedar
oil
Par-focal
Distance
(mm)
45
45
45
45
Magnification
Marks
(color ring)
Red
Yellow
Light blue
Deep blue
45
White
45
45
45
Red
Yellow
Light blue
45
White
6-2 Eyepieces
Type
Magnification
Field of view
(mm)
Wide field eyepiece
10X
15X
20X
5X
ф18
ф18
ф13
ф11
17
Plan eyepiece
10X
16X
ф18
ф15
6. Parameter/technical terms
490 SERIES
6-3 Electrical system
There are two options for electrical systems. Both are equipped with
6V/20W halogen lamps.
(1) 220V∼240V power supply: 220V∼240V ±10%, 50Hz
This electrical system is CE and GS certified
(2) 100V∼120V power supply: 100V∼120V ±10%, 60Hz
This electrical system is UL certified
6-4 Parameters
·
(1) Total magnification:
20X∼1600X
(2) Field of view:
ф0.8mm∼ф4.5mm
(2) Mechanical tube length:
160mm
(4) Object to primary image distance:
195mm
(5) Fine focusing sensitivity:
0.002mm
6-5 Technical terms
(1) Total magnification= magnification of objective multiplied by magnification of eyepiece
(2) Field of view= line ﬁeld of view of the eyepiece divided by the
magniﬁcation of the objective
(3) Object to primary image distance= n·sinα (max), N.A. is an important
parameter that marks the features of the objective and condenser. The “n”
is the refractive index of the medium (air or immersion oil) between the
18
6. Parameter/technical terms
490 SERIES
objective lens and the specimen. The “α ” is 1/2 the aperture angle.
The larger the N.A., the high the resolution of the objective
(4) Object to primary image distance = the distance between the
object plane to the primary image plane. The conjugate distance is
fixed
(5) Mechanical tube length = the distance between the objective
shoulder and the ocular shoulder
19
7. Troubleshooting
490 SERIES
Troubleshooting for the 490 Series Biological Microscope
SYMPTOM
CAUSE
REMEDY
OPTICAL ISSUES
One side of the
field of view is
darker
The nosepiece is misaligned
Turn the nosepiece until it
clicks into place
Stain or dust has accumulated on the
condenser, object, eyepieces, or base
lens
Clean the lens
Obstructions Stains has accumulated on the
are observed in specimen
the field of
Stains have accumulated on the lens
view
There is no cover glass on the slide
The cover glass is not standard
The specimen is upside down
The immersion oil has accumulated on
the dry objective
Unclear
Image
One side of the
field of view is
dark or the
image moves
while focusing
The field of
view is not
bright enough
No immersion oil used with the 100X
objective
Clean the specimen
Clean the lens
Add the cover glass
Use glass w/0.17mm thickness
Flip it over
Clean lens thoroughly
Use immersion oil
Air bubble in the immersion oil
Used wrong oil
The aperature is not open to an
appropriate diameter
Pop the air bubbles
Use standard cedarwood oil
Stain or dust has accumulated on the
lens in the inlet of the head
Clean the lens
The condenser is not in the right position
Adjust the condenser
The specimen slide is not fixed
Secure slide to the stage with
clips
The nosepiece is not in the right position
Turn the nosepiece until it
clicks into place
The iris diaphragm is not big enough
Adjust the diaphragm
The condenser is not in the right position
Adjust the condenser
Adjust the iris diaphragm
Stain or dust has accumulated on the
Clean the lens
condenser, objective, eyepieces, or base lens
20
7. Troubleshooting
490 SERIES
Troubleshooting for the 490 Series Biological Microscope cont’d...
SYMPTOM
CAUSE
REMEDY
OPTICAL ISSUES
The color of the The brightness-adjustment knob is not
in the right position
image is not
accurate
No filter is used
Adjust the brightnessadjustment knob
Use desired filter
MECHANICAL ISSUES
The objective
touches the
cover glass
The cover glass is upside down
Flip the cover glass over
The cover glass is not standard
Use glass w/0.17mm thickness
Unable to
move the slide
smoothly
The slide is not secured correctly
Adjust the slide
The mechanical stage is not properly secured Tighten the mechanical stage
Focus-knob
does not turn
Tension knob is too tight
Loosen it a little
Stage declines
by itself
The tension knob is too loose
Tighten it a little
The coarsefocusing knob
won’t raise the
stage
Limit-stop knob locked
Loosen the limit-stop knob
and lock it after refocusing
The finefocusing knob
won’t raise the
stage
Limit-stop knob locked
Loosen the limit-stop knob
and lock it after refocusing
ELECTRICAL ISSUES
The bulb
flickers
The
microscope
does not light
up
The bulb is close to burning out
The microscope is unplugged
Insert it correctly
The bulb is not inserted correctly
Check the power cable ‘s plug
The bulb burned out
Replace the bulb
The fuse burned out
Replace the fuse
The voltage is too high
Use the correct power supply
or get a voltage adapter
Use correct power supply
Used wrong bulb
Replace w/correct bulb
The fuse burns The voltage is too high
out frequently
The bulb burns
out frequently
Replace the bulb
21