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Package ‘shinyMethyl’
November 10, 2014
Title Interactive visualization for Illumina's 450k methylation arrays
Version 1.0.0
Depends methods, BiocGenerics (>= 0.3.2), shiny (>= 0.9.1), minfi (>=
1.6.0), IlluminaHumanMethylation450kmanifest, matrixStats, R
(>= 3.0.0)
Imports RColorBrewer
Suggests shinyMethylData, minfiData, BiocStyle, RUnit, digest, knitr
Description Interactive tool for visualizing Illumina's 450k array data
Url https://github.com/Jfortin1/shinyMethyl
VignetteBuilder knitr
License Artistic-2.0
biocViews DNAMethylation, Microarray, TwoChannel, Preprocessing,
QualityControl
Author Jean-Philippe Fortin [cre, aut],
Kasper Daniel Hansen [aut]
Maintainer Jean-Philippe Fortin <[email protected]>
R topics documented:
shinyMethyl-package . . .
runShinyMethyl . . . . . .
shinyMethylSet-class . . .
shinySummarize-methods .
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runShinyMethyl
shinyMethyl-package
Interactive visualization for Illumina’s 450k methylation arrays
Description
Interactive tool for visualizing Illumina’s 450k array data
Author(s)
Jean-Philippe Fortin <[email protected]>
Run the interactive shinyMethyl session
runShinyMethyl
Description
Function to run the interactive shinyMethyl session from a shinyMethylSet.
Usage
runShinyMethyl(shinyMethylSet1, shinyMethylSet2 = NULL)
Arguments
shinyMethylSet1
shinyMethylSet that must be extracted from an RGChannelSet
shinyMethylSet2
optional shinyMethylSet that must be extracted from an GenomicRatioSet
Value
No value returned. Instead the shinyMethyl interactive session is launched.
Author(s)
Jean-Philippe Fortin
See Also
shinyMethylSet
shinyMethylSet-class
3
Examples
## Not run:
if (require(minfiData)){
baseDir <- system.file("extdata", package = "minfiData")
targets <- read.450k.sheet(baseDir)
targets$Sample_Plate <- substr(targets$Slide,1,7)
RGSet <- read.450k.exp(base = baseDir, targets = targets)
summarized.data <- shinySummarize(RGSet)
runShinyMethyl(summarized.data)
}
## End(Not run)
shinyMethylSet-class
shinyMethylSet instances
Description
This class holds summarized data from Illumina methylation microarrays for interactive visualization purpose.
Usage
## Constructor
shinyMethylSet(sampleNames = new("character"),
phenotype = new("data.frame"),
mQuantiles = new(vector("list",5)),
betaQuantiles = new(vector("list",5)),
methQuantiles = new(vector("list",5)),
unmethQuantiles = new(vector("list",5)),
cnQuantiles = new(vector("list",5)),
greenControls = new(vector("list",12)),
redControls = new(vector("list",12)),
pca = new("list"),
originObject = new("character"))
## Data extraction / Accessors
## S4 method for signature shinyMethylSet
getMeth(object)
## S4 method for signature shinyMethylSet
getUnmeth(object)
## S4 method for signature shinyMethylSet
4
shinyMethylSet-class
getBeta(object)
## S4 method for signature
getM(object)
## S4 method for signature
getCN(object)
## S4 method for signature
pData(object)
## S4 method for signature
sampleNames(object)
shinyMethylSet
shinyMethylSet
shinyMethylSet
shinyMethylSet
Arguments
object
A shinyMethylSet.
sampleNames
A character vector
phenotype
A data.frame object.
methQuantiles
A list of 5 matrices containing several quantiles for the methylation values (between zero and infinity) separated by probe tpye and autosomal/sex probes.
Each row is a quantile and each column is a sample.
unmethQuantiles
Similar to Meth
betaQuantiles
Similar to Meth but for Beta-values
mQuantiles
Similar to Meth but for M-values
cnQuantiles
Similar to Meth but for Copy Number
greenControls
A list containing the matrices of different raw control probes intensities in the
green channel
redControls
Similar to greenControls but for the red channel
pca
List containing the PCA scores for the 20,000 most variable CpGs and the percentages of variance explained
originObject
Name of the minfi object from which the data were extracted
Details
These class is a representation of a Meth matrix and a Unmeth matrix linked to a pData data frame.
A creationMethod slot is present to indicate from which object type the shinyMethylSet has been
created: either a RGChannelSet or GenomicRatioSet
A MethylSet stores meth and Unmeth.
Constructor
Instances are constructed using the shinyMethylSet function with the arguments outlined above.
shinySummarize-methods
5
Accessors
In the following code, object is a shinyMethylSet.
getMeth(object), getUnmeth(object) Get the Meth or Unmeth matrix.
getBeta(object) get Beta, see details.
getM(object) get M-values, see details.
getCN(object) get copy number values which are defined as the sum of the methylation and
unmethylation channel.
sampleNames(object) Get the sampleNames
combine(object1,object2) combine shinyMethylSet objects
Author(s)
Jean-Philippe Fortin <[email protected]>
See Also
Objects of this class are typically created by using the function shinySummarize on a RGChannelSet
or GenomicRatioSet.
Examples
showClass("shinyMethylSet")
shinySummarize-methods
Summarizing a large 450K experiment into a shinyMethylSet
Description
Summarizing methylation data from a RGChannelSet or GenomicRatioSet into a shinyMethylSet
needed to launch the interactive interface of shinyMethyl.
Usage
## S4 method for signature RGChannelSet
shinySummarize(object)
## S4 method for signature GenomicRatioSet
shinySummarize(object)
Arguments
object
Either a RGChannelSet or a GenomicRatioSet.
6
shinySummarize-methods
Details
Either a RGChannelSet is transformed into a shinyMethylSet, or a GenomicRatioSet is transformed to a shinyMethylSet
Author(s)
Jean-Philippe Fortin <[email protected]>
See Also
shinyMethylSet for the output object and RGChannelSet or GenomicRatioSet for the input object.
Examples
if (require(minfiData)){
library(minfiData)
summarized.data <- shinySummarize(RGsetEx)
}
Index
∗Topic methods
shinySummarize-methods, 5
∗Topic package
shinyMethyl-package, 2
sampleNames,shinyMethylSet-method
(shinyMethylSet-class), 3
shinyMethyl (shinyMethyl-package), 2
shinyMethyl-package, 2
shinyMethylSet, 2, 6
combine (shinyMethylSet-class), 3
shinyMethylSet (shinyMethylSet-class), 3
combine,shinyMethylSet,shinyMethylSet-method shinyMethylSet-class, 3
(shinyMethylSet-class), 3
shinySummarize, 5
shinySummarize
GenomicRatioSet, 5, 6
(shinySummarize-methods), 5
getBeta (shinyMethylSet-class), 3
shinySummarize,GenomicRatioSet-method
getBeta,shinyMethylSet-method
(shinySummarize-methods), 5
(shinyMethylSet-class), 3
shinySummarize,RGChannelSet-method
getCN (shinyMethylSet-class), 3
(shinySummarize-methods), 5
getCN,shinyMethylSet-method
shinySummarize-methods, 5
(shinyMethylSet-class), 3
show,shinyMethylSet-method
getGreenControls
(shinyMethylSet-class), 3
(shinyMethylSet-class), 3
getM (shinyMethylSet-class), 3
getM,shinyMethylSet-method
(shinyMethylSet-class), 3
getMeth (shinyMethylSet-class), 3
getMeth,shinyMethylSet-method
(shinyMethylSet-class), 3
getPCA (shinyMethylSet-class), 3
getRedControls (shinyMethylSet-class), 3
getUnmeth (shinyMethylSet-class), 3
getUnmeth,shinyMethylSet-method
(shinyMethylSet-class), 3
orderByName (shinyMethylSet-class), 3
pData (shinyMethylSet-class), 3
pData,shinyMethylSet-method
(shinyMethylSet-class), 3
RGChannelSet, 5, 6
runShinyMethyl, 2
sampleNames (shinyMethylSet-class), 3
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