Danube Soya – Field Day Markus Kainz Romer Labs Services and Products Mycotoxins Pathogens Allergens GMO‘s Drug Residues & Adulterants A Typical Analytical Procedure Sampling Grinding/ Subsampling Extraction Sampling plan RAS Mill / blender Shaking Purification Clean up / settling Analysis LFD, ELISA, PCR, … What is a GMO? • Insertion of DNA material from outside the organism into the plant's DNA sequence • Confer desired traits on plants: Herbicide tolerance and/or virus or insect resistance Terminology Example: Roundup Ready - AgraStrip GMO RUR • Event name: Unique DNA recombination event NK603 • Trade name: Roundup Ready (Monsanto) • Trait: Novel characteristic = Glyphosate herbicide tolerance • Inserted Gene: Responsible for novel trait CP4 EPSPS • Protein detected: Protein encoded by the inserted gene and detected by Strip Tests - CP4 EPSPS • Rapid test: AgraStrip RUR Bulk Grain - TraitChek Why test for GMOs? Necessary for many producers/traders/manufacturers to • meet specified regulatory requirements • meet commercial contract requirements • ensure internal quality control Regulatory requirements EU: • which GM events may be cultivated • which GM events may be imported for food/feed • under which conditions labeling is required for products containing genetically modified ingredients GMO - Analysis Testing Options DNA: • The introduction of foreign DNA into a recipient plant’s DNA (genetic engineering) • Equipment: PCR, Gel-electrophoresis • Laboratory Environment Protein: • The information coded in DNA is translated into protein that performs the function specified by the DNA • Antibodies for detection • Inexpensive equipment Genetic Analysis (DNA analysis) • Genetic analysis is a GMO testing method that detects the presence of a transgene in a plant cell’s genome • The specific GMO test used in this method is called the Polymerase Chain Reaction (PCR Test) • Performed in a laboratory setting • Appropriate for qualitative or quantitative testing • Highly sensitive and specific Immuno-analysis (Protein analysis) Strip test (Lateral Flow Device or Dipstick) • • • • • A rapid antibody-based method Measuring GMO protein in unprocessed material such as seed, grain, or leaves Immobilized GMO protein-specific antibodies on a solid strip Appropriate for qualitative or semi-quantitative testing Suitable for field testing ELISA Test (Enzyme-Linked Immunosorbent Assay) • • • • • An antibody-based method Measuring GMO protein in unprocessed material such as seed, grain, or leaves Immobilized GMO protein-specific antibodies in a multi-well solid plate format Appropriate for qualitative or quantitative testing Performed in a laboratory setting Strip Test (Lateral flow device) Qualitative Test Kit Semi-quantitative Test Kit Strip Test Options for Soy Test Kit Name Item Number Protein detected Detection Level Matrix AgraStrip® RUR Bulk Grain TraitChek 7000014 CP4 EPSPS 0.1 % Soy AgraStrip® Soy Comb 2 Traits 7880623 CP4 EPSPS, PAT 0.125%, 0.5% Soy AgraStrip® RUR Toasted Meal 7120050 CP4 EPSPS 0.9 % Toasted soy meal 7000014 CP4 EPSPS 0.1 to 4.0 % Soy Qualitative Semi-quantitative AgraStrip® RUR Bulk Grain TraitChek Thank you! Markus Kainz www.romerlabs.com 16