WORKSHOP PROGRAM
9:30 - 17:00
MONDAY
9:30 Opening / introduction
Why cryo-TEM?
prof.dr. N. Sommerdijk
10:45 Lecture
dr. W. Busing / dr. M. Vos
Principles and technical applications of cryo-TEM
12:30 Lunch
13:30 Instruction
TUESDAY
9:30 Hands on: Cryo-TEM
12:30 Lunch
13:30 Hands on: Cryo-TEM
WEDNESDAY
9:30 Lecture: Tomography
dr. H. Friedrich
Principles and technical application of electron tomography
10:45 Demonstration: Tomography
12:30 Lunch
13:30 Hands on: Tomography
Hands on workshop on data acquisition for
cryo-electron tomography
19:00 Dinner in town
THURSDAY
9:30 Lecture: Reconstruction
dr. M. Vos
Part 1: Electron tomographic reconstruction
10:45 Demonstration
dr. H. Friedrich
Part 2: IMOD tutorial
12:30 Lunch
13:30 Hands on:
FRIDAY
9:30 Lecture
prof.dr. N. Sommerdijk
Soft Matter Cryo TEM
Cryo-TEM in Material Science
10:45 Evaluation / Closing
12:30 Lunch
13:30 Departure
Acknowledgement
Cover: Reprinted with permission from A.L. Parry et.al. Angewandte Chemie
Int. Ed. 47 [46] (2008) 8859, fig 2 TEM analysis of aggregates of PNOEGPNLVL. Copyright 2008 Wiley-VCH Verlag GmbH & Co.
Page 2: Top: Reprinted with permission from G.A.F. van Tilborg et.al. Magnetic Resonance in Medicine 60 (2008) 1444-1456, fig 2B Cryo-TEM images
of biotinylated paramagnetic liposomes. Copyright 2008,John Wiley and
Sons.
Bottom: Reprinted with permission from M.J.M. Wirix et.al., NanoLetters 14
(2014) 2033, abstract fig, Cryo-ET 3D visualization of P3HT, Copyright 2014
American Chemical Society.
Page 2-3 bottom: Titan TEM: Photo Archive TU/e.
Page 3: Top: Angewante Chemie: Cover picture, Cryo electron reveals confined complex morphologies of tripeptide-containg amphiphilic double-comb
diblock copolymers (Angew.Chem.Int.Ed. 46/2008 page 8751). Copyright
2008 Wiley-VCH Verlag GmbH & Co.
Middle: Vitrobot, Photo Archive FEI Company.
FEI Company
Eindhoven
www.FEI.com
Eindhoven University of Technology
Laboratory of Materials and
Interface Chemistry
Helix Building STO 2.91
Het Kranenveld 14 / P.O. Box 513
5600 MB Eindhoven / The Netherlands
Www.chem.tue.nl/smg
www.cryotem.com
3rd International Cryo-TEM Workshop
April 13th – April 17th 2015
Eindhoven
Soft Matter Cryo-TEM
3 R D INTERNATIONAL CRYO-TEM WORKSHOP 2015
Advancements in cryo-transmission electron micros-
Using cryo-TEM and cryo-electron tomography (3D
copy (cryo-EM) have made it possible to image ob-
cryo-TEM) we have revealed the structure and mor-
jects from biological origin, such as viruses and pro-
phology of assemblies of surfactants, lipids and (bio)
teins, now also reaching near atomic resolution. In
polymers in solution, as well as at interfaces. But
[email protected]
recent years cryo-TEM is also increasingly used to
cryo-TEM also allows us to study the interactions
Phone +31 40 247 5870
investigate soft matter of synthetic origin: assem-
between organic and inorganic components, e.g. in
blies of organic (macro) molecules and/or inorganic
the mineralization of collagen, generating artificial
nanoparticles.
bone-like materials.
Since almost a decade, Eindhoven University of
With this workshop we want to demonstrate the pos-
Technology and FEI company have worked together
sibilities of cryo-TEM, and to show you how to use
at the forefront of this new field trying to advance
this powerful technique in your own research and in
cryo-TEM in materials science, both through devel-
your own laboratory. The workshop is aimed at par-
oping dedicated microscopes but also through de-
ticipants at PHD/post doc level and who are experi-
veloping advanced tools and methods for sample
enced in basic microscopy and who wish to extend
preparation and imaging. This has allowed us to
their knowledge and practical experience in 2D and/
investigate a variety of soft and hybrid materials in
or 3D cryo-imaging. The work shop will consist of
their native hydrated state with nanometer resolution
lectures from experts, but also of extensive practical,
both in two and three dimensions.
hands-on sessions where you will be stimulated to
ORGANIZATION
P R O G R A M H I G H L I G H TS
• TEM OF SOFT MATTER: WHY CRYO-TEM?
• SAMPLE PREPARATION: VITRIFICATION
• LOW DOSE IMAGING
•INTERPRETATION OF IMAGES
Prof. Nico Sommerdijk - TU/e -
Dr. Matthijn Vos - FEI Company [email protected]
Phone : +31 6 2351 5172
investigate samples from your own research. .
Dr. Wim Busing - FEI Company [email protected]
Phone: + 31 40 235 6115
REGISTRATION
The registration fee is € 1750,‐ per person, including full program, lunches and one dinner. REGISTER on www.cryotem.com • 2D VS 3D TOMOGRAPHY
Visualizing your samples
in their native hydrated state
• DATA ACQUISTION
Closure registra on 3 April 2015. WHEN
CRYO-TEM SOLUTIONS
MICROSCOPY AND SAMPLE PREPARATION
CryoT-EM will allow you to visualize solu-
Imaging soft matter poses a number of technical chal-
tion species in their native hydrated state,
lenges related to the delicate nature of the material of
so without running the risk of introducing
interest, its low contrast in the specimens, in combination
artifacts due to staining or drying. By
with the high vacuum and low temperature requirements
plunge freeze vitrification soft matter sam-
for the cryo-TEM process. You will work with our micro-
ples, are embedded in an amorphous ice
scope (TitanKrios) optimized for combined resolution
layer that fixates and stabilizes them so
and contrast as well as with our vitrification robots
their structure and morphology can be
(Vitrobot) for efficient and reliable sample preparation.
studied, in principle with nanometer detail.
April 13th — April 17th 2015 WHERE
Eindhoven University of Technology
Department Chemical Engineering
Cryo-TEM routinely uses low-dose proto-
A P P LI C A TIO N M A NA GE M E N T
cols to control the exposition of the sam-
In recent years, time resolved 2D and 3D
Helix Building Room STO 2.91
ple to the electron beam, preventing radi-
Cryo-TEM has led to new insights in a variety
Het Kranenveld 14 / P.O. Box 513
ation damage to the generally beam sen-
of soft mater systems both in aqueous media
5600 MB Eindhoven
sitive soft matter.
and organic solvents. You will be shown how
The Netherlands the horizon of cryoT-EM can be widened by
developing new procedures and exploring the
limits of the current sample preparation techniques.
Lab. Materials and Interface Chemistry