1. business and industrial
2. agriculture and forestry
3. crops and seed

IJPRD, 2014; Vol 6(10):December-2014 (119 - 122)
International Standard Serial Number 0974 – 9446
-------------------------------------------------------------------------------------------------------------------------------------------------EFFICACY OF SOME PLANT EXTRACTS AGAINST ROSE APHID, MACROSIPHUMROSAEFORMES (DAVIS)
Manisha Gadakh*1
1*
Faculty, Department of Zoology,S.M.B.S.T.College, Sangamner, Ahmednagar – 422605
Correspondence Author
ABSTRACT
Aqueous extract of various plants has been tested for their
insecticidal
(aphidal)
efficacy
against
rose
aphid,
Macrosiphumrosaeformes (Davis) in laboratory conditions.
Thirteen plant extracts with standard insecticidal and untreated
control were tested. Garlic, Allium sativum (Linn),was found to be
best effective followed by Custard apple, Annonasqumosa (Linn)
and Bullock heart, Annonareticulate(Linn).
Keywords- Plants extracts, aphidicidal activities, laboratory
conditions.
Manisha Gadakh
Faculty, Department of
Zoology,S.M.B.S.T.College,
Sangamner, Ahmednagar – 422605
Email: [email protected]
INTRODUCTION
In Rose aphid, Macrosiphumrosaeformes (Davis) is
a key pest of rose,Rosa canine (Linn).This pest
causes damage directly by sucking the sap from
different parts of the plants and indirectly by
transmitting the viral diseases.Heavy infection of
this aphid can causes considerable damage to the
rose and reducesquality of flower.A number of
effective insecticides have been reported for the
control of rose aphid.But it is costly and dangerous
to ecosystem.For the control of aphid pest various
insecticides are recommended and regularly used.
But it gives side effect pest resistance (Metcalf,
1988),secondary pest outbreak (Oka and
Pimentel,1974), encountered infood animal
products adipose tissue and milk (Brown,1974),
and
effect
on
non-target
organism
(Meherotra,1988). Present study was undertaken
to evaluate the base data on plant based
insecticide specificallyaphidicidal on the given pest
which are chief and ecofriendly.
MATERIALS AND METHODS
A laboratory experiment was conducted at the
Department of Zoology, of S.M.B.S.T College,
Sangamaner during the year 2013-2014.The rose
aphid, M. roseformesand rose twigs were brought
to laboratory from field. Various thirteen plant
Available online on www.ijprd.com
119
International Journal of Pharmaceutical Research & Development
species shown in Table1 were selected for
aphidicidal activity test. Plant leaves were firstly
washed 2-3 times in sterilized water. 10gms leaves
material in 10 ml distilled water.Extracts were
allowed to evaporate at room temperature. These
materials were squeezed through double layer
muslin cloth and paper (Watman No.1).
For the experimentation 20 healthy, non pathologic
and same size aphids were used for each treatment
one ml of each extract was applied on young twigs
and aphids in Petri plates.The mortality was
recorded after 12,24,36,48 and 60 hrs of post
treatment. Recorded data subjected to statistical
analysis after regular transformation.
RESULTS AND DISCUSSION
It was seen from the present study that the
mortality in the treated aphid was occurred due to
treatment of respective products.The data
presented in Table2 indicates that the percent
mortality of various plants on aphid at
12,24,36,48,and 60 hours post treatment and study
Sr no
ISSN: 0974 – 9446
revealed high percent mortality in Garlic, Allium
sativum
and
followed
at
par
Bullock
heart,Annonareticulata. The extract of garlic
showed highest mortality to compared standard
control mortality at 12,24,36,48 and 60 hours posttreatment
respectively.Adathodavasica
was
observed to be effective against, safflower aphid
,U.compositae (Dhembareet. al, 1998). A leaves
powder of A. Vasicawas effective on store grain
pest(Shrivastava et al. 1964). Pandeyet.al,(1987)
reported that O. santumwas better treatment
against, L. Erysimi and also Dhembare and
Shekatkar(1994) on the same aphid species.
Other plant extracts those showed least percent
mortality at different hours of post-treatment but
these plant extracts might have some other
properties such as attaractant, repellent,
chemosterilant,
fungisidal,
nematocidal,
bactericidal, rodanticident. But it was beyond the
scope of the present study. It was mainly directed
towards the aphidicidal and find out baseline data.
Table 1 Showing plant species used for insecticidal properties.
Plant species
Family
Comman name
Treatment number
1
Allium sativum
Liliaceae
Garlic
1
2
Annonareticulata
Annonaceae
Bullock heart
2
3
Annonasquamosa
Annonaceae
Custard apple
3
4
Azadiractaindica
Meliaceae
Neem
4
5
Cassia ouriculata
Caesalpinaceae
Tarwar
5
6
Catharanthusroseas
Apocynaaceae
Sadaphuli
6
7
Indigoferatinctoria
Fabaceae
Indigo
7
8
Ipomefistulosa
Convulvulaceae
Besharme
8
9
Menthaspicata
Lamiaceae
Mentha
9
10
Moringaoleifera
Moringaceae
Shevaga
10
11
Murrayaskoengi
Rutaceae
Kadipatta
11
12
Neriumindicum
Apocynaceae
Kanher
12
13
Osciumumsantam
Labiateae
Tulsi
13
14
Endosulfan 35% EC
------------
Thiodon
14
15
Untreated control
---------
--------
15
Available online on www.ijprd.com
120
International Journal of Pharmaceutical Research & Development
Treatment
no
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
X
S.D
C.V
ISSN: 0974 – 9446
Table 2 Showing per cent mortality of rose aphid after post treatment.
Percent mortality (Hrs)
12 hrs
15.00
(3.93)
12.00
(3.53)
10.00
(3.24)
9.00
(3.08)
5.00
(2.39)
7.00
(2.73)
8.00
(2.91)
6.00
(2.54)
5.00
(2.34)
0.00
(0.70)
5.00
(12.34)
8.00
(2.91)
5.00
(2.34)
40.00
(6.36)
0
5.71
11.88
20.79
24 hrs
36 hrs
48 hrs
20.00
35.00
45.00
(4.52)
(5.95)
(6.744)
18.00
25.00
36.00
(4.30)
(5.04)
(6.04)
15.00
20.00
32.00
(3.93)
(4.52)
(5.70)
14.00
21.00
30.00
(3.80)
(4.63)
(5.52)
12.00
24.00
28.00
(3.53)
(4.93)
(5.33)
10.00
15.00
20.00
(3.24)
(3.93)
(4.52)
15.00
25.00
32.00
(3.93)
(5.04)
(5.70)
13.00
25.00
32.00
(3.67)
(5.04)
(5.70)
8.00
25.00
28.00
(2.91)
(5.04)
(5.33)
10.00
15.00
20.00
(3.24)
(3.93)
(4.52)
10.00
15.00
25.00
(3.24)
(3.93)
(15.04)
10.00
15.00
25.00
(3.24)
(3.93)
(15.04)
15.00
20.00
25.00
(3.930
(4.52)
(5.04)
70.00
100.00
(8.39)
(10.02)
-----0
0
0
9.71
21.14
23.90
20.44
32.21
22.64
21.04
11.86
94.7
Figures in the parenthesis are poison value.
REFERENCES
1. Amorkar, S. V. (1986) Microbial control of
insect pest of economic importance using
Bacillus thurineginesis. Var. Proc. Increasing
crop productivity, Bombay,June, 20-21: 383400.
60 hrs
60.00
(7.77)
55.00
(7.44)
50.00
(7.10)
45.00
(6.74)
35.00
(5.95)
30.00
(5.52)
40.00
(6.36)
40.00
(6.36)
30.00
(5.52)
30.00
(5.55)
35.00
(5.95)
40.00
(6.36)
35.00
(5.95)
-----0
37.14
26.37
17.00
2. Meherotra, K. N. (1986)Newtrents in use of
insectides. Proc. Increasing crop productivity,
Bombay, June 20-21:353-369.
3. Brown, A. W. A. (1978) Ecology of pesticids.
John Wiley and sons. New York.pp 525.
4. Dhembare, A. J. and S.Sheketkar (1994). Effect
of some plant extracts against Lipaphiserysimi
Available online on www.ijprd.com
121
International Journal of Pharmaceutical Research & Development
(Kalt) under laboratory conditions. Paper
presented
at
Seminar
on
EnvironmentalPolltion; A threat to future
sustainable life o the earth, Pravaranagar, on
March,20-21:pp21.
5. Metacalf, R. L. (1980) Changing role of
insecticides in crop protection. Ann. Rev. Ent.
25:219-256.
6. Oka, I. N. and Pimental (1974) Corn susceptible
to corn leaf aphid and common corn smut alter
herbicide treatment.Envirn.Ent. 3(6): 911-915.
7. Pandy, N. D., L. Singh, Y. P. Singh, and R.
A.Tripathi (1987). Effect of certain plant
extracts against
ISSN: 0974 – 9446
8. L.erysini (Kalt) under laboratory conditions.
Indian Jour. Ent. 49 (2): 238-242.
9. Shrivastava, A. S., H. P.Saxsena, D. R. Singh
(1968)
Role
of
indigenous
insecticidsAdathodavasica. Incontrolling major
insect pests of oilseed during storage. Ent. Res.
Work Conf. IARI,New Delhi. April 22-24,pp.49.
10. Dhembare, A. J., G. M.Pondhe, R. D.Kharde, and
J. A.Dhumal (1998). Life table studies and
control of saffloweraphid by plant products. J.
Maha Agric. Univ.23(1):1-3.
*****
Available online on www.ijprd.com
122