Young Researcher-Talks Himani Singh, Naveen Kumar, A. K.

Young Researcher-Talks
~-12
IN VIVO CONNECTIVE TISSUE REMODELING BY MMP-9
Himani Singh, Naveen Kumar, A. K. Sharma, Amit Kumar and vineet kumar
Division of Surgery,lndian Veterinary Research Institute, lzatnagar-243122, Uttar
Pradesh, India,
Presenting author e-mail: h.singh20 [email protected]
Matrix metalloproteinases (MMPs)
represent a group of enzymes involved in the degradation of extracellular matrix and therefore participate
in tissue remodeling. The aim of this
work was to analyse the pre.sence
of MMP-9 in rabbit blood plasma
before and after subcutaneous implantation with native and acellular
buffalo pericardium, crosslinked with
and without O.So/o Glutaraldehyde
(GA) and O.So/o Hexamethylene diisocyanate (HMDC). One part of buffalo
pericardium was cross-linked as such
while another part was made acellular and then cross-linked. Blood plasma was collected just before operation
(O day) and at 15, 30, 60 and 90 days
post-operation using heparin (1 0-20
IU/ml) through heart. The MMP-9 was
determined by using gelatin zymog-
raphy and western blotting. Quantitative estimation of MMP-9 (92 kDa)
by ELISA showed that HMDC crosslinked native and acellular groups
expressed significantly increase level
of MMP-9 as compared to uncrosslinked and GA cross-linked groups.
Western blot study observed HMDC
crosslinked pericardium groups expressed prominent bands of 135 and
92 kDa (dimeric form of MMP-9) at
day15 as compared to zero, 30, 60
and 90 days. Gelatin zymography
was also expressed prominent band of
92 kDa in HMDC crosslinked acellular
pericardium group as mmpared toGA
crosslinked groups. Result was indicating that HMDC crosslinked acellular buffalo pericardium contribute to
the in vivo collagen degradation and
tissue remodeling.
135