1. No category

ASTM F1608 Standard Test Method for
Microbial Ranking of Porous Packaging
Materials ( Exposure Chamber Method).
Agenda
• Basic overview of the test method
• Proposed changes for the future.
Microbial Ranking Test - ASTM F1608
Determines the Log Reduction Value (LRV) of
porous material
Packages placed into
Sample size must be
Filters are blended
a chamber and
a minimum of 47mm
and plated to
exposed to a specific
in width
determine titer values
organism (Bacillus)
Copyright Nelson Laboratories 2014
3
How it works..
Microbial Ranking Test
Copyright Nelson Laboratories 2012
9
Supplies
•
•
•
•
•
55 Soybean Casein Digest Agar Plates
500 mL Soybean Casein Digest Broth
1000 mL Sterile Water
7 (0.45 um) Nitrocellulose filters
Plating equipment
3 key proposed changes
1) Equipment availability
2) Chamber evacuation process.
3) Titration methods
1) Standard equipment issue…
• DeVilbiss #40
Nebulizer no
longer
available.
2) Exposure chamber evacuation
“After exposure, turn off
the vacuum, and the fan.
Evacuate the chamber for
15 min by connecting the
vacuum source to the
chamber through a
microbial filter assembly”
3) Titration methods
0.6 Weight Meltblown
Reference Material
Tyvek
0.45µm Pre-sterilized
Filters
Extraction Method
Blended
Vortex (10-1
Only)
Vortex including
Direct Plating
Blended
Vortex
Blended
Vortex
Standard Deviation
0.8
0.6
0.6
0.2
0.2
0.2
0.1
Reproducibility
2.2
1.8
1.6
0.7
0.6
0.5
0.4
Thank you