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20th Annual Midwest Stress Response
and
Molecular Chaperone Meeting
Saturday, January 17, 2015
Northwestern University
James L. Allen Center, Evanston, IL
McCormick Tribune Auditorium
Program Chairs
Veena Prahlad, University of Iowa
Kevin Morano, The University of Texas Medical School at Houston
Meeting Organizers
Richard I. Morimoto, Northwestern University
Georgette Pliml, Northwestern University
This meeting is generously supported by
The Daniel F. and Ada L. Rice Institute for Biomedical Research
7:30–8:55 AM
Continental Breakfast
1st Floor Lounge (located behind the McCormick Tribune Auditorium)
8:55-9:00 AM
Opening remarks
McCormick Tribune Auditorium
Stress in the cell: protein folding and (mis)folding
Session Chair: James West, The College of Wooster
9:00-9:20 AM
Faces in the Crowd: Disparate Mechanisms of Protein Stabilization by Crowding
and Chemical Chaperones
Shahar Sukenik and Martin Guebele
Dept. of Chemistry, University of Illinois at Urbana-Champaign, IL
9:20-9:40 AM
Interactions Between Ribosome-Bound Nascent Proteins and the Ribosomal
Surface
Valeria Guzman Luna, Rayna Addabbo, Yue Liu and Silvia Cavagnero
Dept. of Chemistry, University of Wisconsin, Madison, WI
9:40-10:00 AM
Architecture of the TIM23 inner mitochondrial translocon and interactions
with the Hsp70-based import motor
See-Yeun Ting, Brenda A. Schilke, Masaya Hayashi and Elizabeth A.Craig
Dept. of Biochemistry, University of Wisconsin, Madison, WI
10:00-10:20 AM 343delT Mutant aB-crystallin: Loss or Gain of Toxic Function Using Induced
Pluripotent Stem Cells as a Model System.
Katie Mitzelfelt 1,2, Pattraranee Limphong 2, Qiang Dai1, Melinda Choi1, Michael Riedel1,
Shu Lai1, Elisabeth Christians2, Elizabeth Wraige3, Michael Grzybowski1, Aron Geurts1
and Ivor Benjamin1,2
1
Dept. of Medicine, Medical College of Wisconsin, Milwaukee, WI; 2 Dept. of Biochemistry,
University of Utah, Salt Lake City, UT; 3 Evelina Children’s Hospital, London, UK
10:20-10:40 AM Coffee Break (1st Floor Lounge behind the McCormick Tribune Auditorium)
Stress sensing and signaling
Session Chair: Tali Gidalevitz, Drexel University
10:40-11:00 AM A stress response switch controlled by jmjd-3.1 marks the onset of reproduction in
Caenorhabditis elegans
Johnathan Labbadia and Richard I. Morimoto
Dept. of Molecular Biosciences and Rice Institute for Biomedical Research, Northwestern
University, Evanston, IL
11:00-11:20 AM Neurosensory Regulation of Serotonergic Signaling in the Heat Shock Response in
Caenorhabditis elegans
Felicia Ooi, Marcus Tatum, Madhusudana Chikka and Veena Prahlad
Dept. of Biology, Aging Mind and Brain Initiative, University of Iowa, Iowa City, IA
11:20-11:40 AM Regulating IREl signaling to change the quality of the unfolded protein response
Daniela Eletto, Davide Eletto*, Jeffrey Thomas and Yair Argon
Div. of Cell Pathology, The Children’s Hospital of Philadelphia, and the University of
Pennsylvania, Philadelphia, PA. *Current address: Dept. Medicine, Cambridge University,
Cambridge, UK.
11:40 AM-12:00 PM
Role of HdeB as periplasmic chaperone during acid stress
Jan-Ulrik Dahl1, Philipp Koldewey1,2, Loïc Salmon1,2, Scott Horowitz1,2, James C. A.
Bardwell1,2, and Ursula Jakob1
1
Dept. of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann
Arbor, MI ; 2Howard Hughes Medical Institute, University of Michigan, Ann Arbor, MI
Plenary Talk
Session Chair: Veena Prahlad, University of Iowa
12:00-12:45 PM Proteostasis in Health, Aging, and Disease
Richard I. Morimoto
Dept. of Molecular Biosciences and Rice Institute for Biomedical Research, Northwestern
University, Evanston, IL
12:45-1:45 PM
Lunch
1:45-3:00 PM
Poster Session
Room 153 (behind the McCormick Tribune Auditorium and next to the 1st floor lounge)
Chaperone dynamics
Session Chair: Ahmed Chadli, Georgia Regents University
3:00-3:20 PM
Mutations of Ssal at P417 alter ATP cycling, interdomain coupling, and specific
chaperone functions
Patrick G. Needham and Jeffrey L. Brodsky
Dept. of Biological Sciences, University of Pittsburgh, Pittsburgh, PA
3:20-3:40 PM
Observing Chaperone-Mediated Protein Folding
Scott Horowitz1, Philipp Koldewey1, Loic Salmon1, Raoul Martin1, Logan Ahlstrom2, Lili
Wang1, Henry van den Bedem3, Qinpgping Xu3, Raymond Trievel4, Charles Brooks III2,
Shu Quan1 and James Bardwell1
1
Dept. of Molecular, Cellular, and Developmental Biology, University of Michigan,
and the Howard Hughes Medical Institute; 2Dept. of Chemistry and Biophysics,
University of Michigan; 3Stanford Linear Nuclear Accelerator; 4Dept. of Biological
Chemistry, University of Michigan
3:40-4:00 PM
UPR-regulated dFic reversibly AMPylates BiP during endoplasmic reticulum
homeostasis
Hyeilin Ham1, Andrew R. Woolery1, Charles Tracy2, Drew Stensen2, Helmut Krämer2 and
Kim Orth1
1
Dept. of Molecular Biology, 2Dept. of Neuroscience, University of Texas Southwestern
Medical Center, Dallas, TX
4:00-4:20 PM
Hsc70 controls microtubule dynamics through tau, necessitating disease-specific
dosing strategies with chaperone-based treatments
Sarah N Fontaine1,2, Jennifer N. Rauch3, Bryce A. Nordhues1,2, Mackenzie D. Martin1,
Andrew R. Stothert1, Alison Fertig4, Elias Akoury6, Victoria A. Assimon3, Sergiy Borysov1,
Matt Cockman1, Lyra Chang3, Pengfei Li1, Jonathan J. Sabbagh1,2, Stanley M. Stevens,
Jr.4, Markus Zweckstetter5 , Erik R.P. Zuiderweg6, Jason E. Gestwicki3, Chad A. Dickey1,2
1
Dept. of Molecular Medicine, Byrd Alzheimer's Institute, U. of South Florida, Tampa, FL;
2
James A. Haley Veteran’s Hospital, Tampa, FL; 3Institute for Neurodegenerative Disease,
U. of California at San Francisco, CA; 4 Dept. of Cell Biology, Microbiology and Molecular
Biology, U. of South Florida, Tampa, FL; 5Dept. for NMR-Based Structural Biology, Max
Planck Institute for Biophysical Chemistry, German Center for Neurodegenerative
Diseases (DZNE), Center for Nanoscale Microscopy and Michigan, Ann Arbor, MI
4:20-4:40 PM
Coffee Break (1st Floor Lounge behind the McCormick Tribune Auditorium)
Organismal consequences of cellular stress
Session Chair: Amie McClellan, Bennington College
4:40-5:00 PM
Investigating the potential for evolutionary innovation in Hsp90
Pamela Cote, Ryan Hietpas and Daniel Bolon
Dept. of Molecular Pharmacology and Biochemistry, University of Massachusetts Medical
School, Worcester, MA
5:00-5:20 PM
Identification and Characterization of Small Molecules Targeting FKBP52 as a
Novel Treatment of Prostate Cancer
Naihsuan C. Guy, Paola A. Ramos, and Marc B. Cox
Dept. of Biological Sciences and Border Biomedical Research Center, University of
Texas at El Paso, El Paso, TX
5:20-5:40 PM
Misfolding of an IGF-like protein disrupts ER folding homeostasis in cell-specific
manner
Lauren Klabonski, Lakshana Senthilkumar and Tali Gidalevitz
Dept. of Biological Sciences, Drexel University, Philadelphia, PA
5:40-6:00 PM
Caenorhabditis elegans Models to Study Transthyretin Secretion, Degradation, and
Proteotoxicity
Erin R. Greiner1, Diana Porras-Gonzalez1, Joseph Genereux1, Sylvia Neumann1, Johan
Paulsson1,2, Andrew Dillin2, Jeffery W. Kelly1 and Sandra E. Encalada1,3
1
Dept. of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla,
CA; 2 Howard Hughes Medical Institute, Dept. of Molecular and Cell Biology, University of
California, Berkeley, Berkeley, CA; 3 Dorris Neuroscience Center, The Scripps Research
Institute, La Jolla, CA
6:00-6:10 PM
Closing Remarks
Adjourn to Prairie Moon (1502 Sherman Ave., Evanston)