1. No category

((2011)) E.
((
))
http://www.basra-science-journal.org:
ISSN-1817-2695
Fusarium oxysporum Schl f.sp. lycopersici
/
Elsa
)
/
Pseudomonas fluorescens pf. D.S
(
Fusarium oxysporum Schl
.f.sp lycopersici
Pseudomonas fluorescens
3
F.o.l.
P.D.A.
Elsa
F.o.l
.
.
2.4
3
%100
.
(SA)
+ CuSO4 H2O +F.o.l.
P. fluorescens + Salicylic acid
.
P. fluorescens
.N.P.K
Pseudomonas fluorescens
(19) Phytoalexin
Fusarium oxysporum f.sp. lycopersici :
(18)
(ISR)
PR-)
Induced Systemic Resistance
(34) Pathogensis- related protein (Protein
Activation of
programmed cell death
19
...
:
&
(17) Hypersensitive reaction
endogenous signel
.(20)
Pseudomonas fluorescens
.(12) PR-protien
.(35) Jasmonic acid
.(31) SA
(ISR)
Fusarium oxysporum
lycopersici
O-antigen of lipopolyasacharides
.(23)
.
-2
0.5
.(
/
.
)%
/
/
.
-2:1:2
P.
2009-
fluorescens
F.o.l
.
.2008
( spotting )
-1:2
P.fluorescens
-1:1:2
. F.o.l
KB
9
/
F.o.l
Fusarium f.sp lycopersici
oxysporum
P.D.A.
.
1
.(6) Aghigi
0.45
.(Potato Dextrose Agar) P.D.A.
Cellulos Acetate Fillter
.o 1± 27
Fillter)
.(9) Booth
.(Membranne molecular
(11) Dewan
100
.(14)
.Miliaceum Panicum
10
-3:1:2
Elsa
P.D.A
(1:1)
F.o.l
Elsa
ppm 1000
/
(
(% 50 Carbendazim)
20
/
F.o.l
) % 0.5
...
:
&
100
1 ± 27
P.D.A.
.
50
40
30
P.D.A.
.
10 0
9
0.5
-: (3)
F.o.l
3
–
100 ×
20
=
Elsa
P.fluorescens
F.o.l
P.D.A.
100
%
-4:1:2
CuSO4.5H2O
Salicylic acid
(2)
/
(3:1:2)
.
(5)
/
0.1
0.75
/
/
200
100
Elsa
F.o.l
P.D.A.
SA
× 8
(30)
10
8
ml/ cfu 10 × 8
(14)
10
5
8
/
1000
8
10
10 × 3
.ml/cfu 10
P.fluorescens
P.D.A.
300
200
100
0
.
F.o.l
-5:1:2
100
500 400
(3:1:2)
.
/
-2:2
15
.
15
1:1 )
.
.
:
SA
100 ×
CuSO4.5H2O
=
21
(
...
:
&
:
0
1
2
3
4
:
(24) ( 1996 ) Juber
4×4
Mckenny
+... + 0 × 0
×
100 ×
=
%
:
-1
Spectrophotometer
Riley
Murphy
700
.
(25)
.Flame photometer
-2
:
-5
/
Total Soluble Solide (TSS)
) =
(
.Hand refraactometer
)
100 × (
-3
.
-:N.P.K
-4
Distillation
(27) Page
Unit
-3:2
Arcsine transformation
C.R.D.
R.L.S.D.
0.05
R.C.B.D.
0.01
.(1)
P.
3 3
(1)
F.o.l
(4)
-1:3
fluorescens
F.o.l
2.4
P. fluorescens
. P. fluorescens
F.o.l
22
-3
...
Catalase
:
&
.% 100
Chitinolytic enzyme
.(8)
P. fluorescens
HCN
Phenzain -1- caroxylate
(33)
F.o. l
P. fluorescens
: (1)
( )
P. fluorescens
+++
3
+++
3
+++
2.4
P. fluorescens
0.46
R.L.S.D (0.01)
100
P. fluorescens
10
% 45.56
Elsa
F.o.l
F.o.l
4.90
-2:3
P.D.A.
(1)
(Carbendazim) Elsa
F.o.l
50
%100
% 83.7
1.46
.
(29) Eswaran
F.oxysporum
.
F.o.l
500
Sanjeev
%0
9
f.sp. vasinfectum
(26)
0
.
(16) Gangawane
.F.o.l.
Ozgonen
(SA)
Carbendazim
Benzimedazole
Elsa
.µm 0.6
DNA
.(3)
(3) (2)
F.o.l.
%0
23
9
...
:
&
.(3)
F.o.l
Elsa
F.o.l
(1)
F.o.l
(3)
(2)
%
%
*0
( )
ppm
9.00
Control
0
9.00
100
10
8.1
200
61
3.51
300
73.6
2.37
400
83.7
1.46
500
6.11
0.55
R.L.S.D
(0.01)
( )
ppm
*0
9.00
0
9.00
10
7.11
8.36
20
18.21
7.36
30
29.67
6.33
40
45.56
4.90
50
5.40
0.48
*
Control
R.L.S.D
(0.01)
*
-3:3
pf.
(4)
P. fluorescens DS
)
P. fluorescens pf. DS
F.o.l
(Elsa
24
...
:
&
F.o.l.
.
P.
P.
Cu +SA + fluorescens
(ISR)
fluorescens
%9.92
SA
%11.74
F.o.l
%90
%63.44
.(28) pathogensis related protein
(F.o.l.)
SA
+
P.
Cu+SA+F.o.l
0.638
0.160
) F.o.l.
(
.(22) PR- Protein
0.161
0.054
-4:3
pf.
(4)
P. fluorescens DS
P. fluorescens
(21)
F.o.l
Jinnah
F.o.l
.
P. fluorescens
/
/
%7.69
.Ralstonia solanacearum
81.14
(15) Katan
P.
(5)
P. + SA+ Cu + F.o.l.
/
fluorescens
Gamliel
fluorescens
8.85
(F.o.l.)
%61.59
fluorescens
P.
.
gibberellins
Cytokinins
%80 -65
fluorescen
/
3.5
/
73.36
/
19
.
Indole acetic acid
(32)
) F.o.l
Siderophores
Pyochelin
( Pseudobactin ) Pyovedine
.(10) SA
%0.466
SA
135.2
4. 58
25
1.80
.(
%3.95 K
.F.o.l
El-khallal
4.28
P N
.
%2.40
...
:
&
(13)
.F.o.l
SA
.(7)
P. fluorescens pf.DS
%
( )
(4)
%
( )
0.655
0.180
0
0
0.161
0.782
0.854
1.214
0.730
0.371
0.445
0.054
0.190
0.210
0.226
0.173
0.108
0.122
63.44
0
0
0
0
31.05
20.94
90.00
0
0
0
0
41.72
35.24
F.o.l
Cu
SA
P. fluorescens pf.DS
Elsa
Cu
SA + F.o.l
0.466
0.125
19.22
29.98
P. f + F.o.l
0.378
0.093
24.83
35.24
Elsa
0.425
0.114
17.05
23.49
Cu +P. f+ F.o.l
0.590
0.155
17.05
23.49
SA + P. f + F.o.l
0.525
0.135
14.89
18.23
Elsa +P. f + F.o.l
0.638
0.160
9.92
11.74
Cu +SA +P. f + F.o.l
0.486
0.124
19.22
23.49
Elsa + Cu + SA + F.o.l
0.307
0.103
35.24
48.21
P. f + F.o.l
0.08
0.021
3.25
3.71
26
+ F.o.l
(0.05) R.L.S.D
((2011)) E.
((
))
P. fluorescens pf.DS
(5)
( )
%K
%P
%N
2.47
0.399
4.04
140.1
1.20
0.236
2.69
2.64
0.434
2.81
/
( )
4.60
3.9
71.5
58.4
1.80
1.1
4.20
140.2
4.71
0.480
4.19
144.3
2.87
0.492
4.33
2.78
0.438
1.99
/
%
%
20
0
0
41.3
9.33
61.59
81.14
3.8
73.67
21
0
0
Cu
5.21
4.0
75.63
23
0
0
SA
149.8
6.17
4.2
81.80
25.33
0
0
P. fluorescens pf.DS
4.63
142.4
4.71
3.8
74.00
20.00
0
0
0.364
3.66
114.7
2.93
2.6
60.00
14.00
26.49
35.00
Cu
2.25
0.327
3.75
125.4
3.33
2.9
63.3
15.33
24.57
30.78
SA + F.o.l
2.30
0.349
3.73
127.7
3.67
3.2
65.4
16.00
21.37
26.56
P. f + F.o.l
2.08
0.304
3.21
114.8
3.00
2.5
57.30
14.67
25.57
30.78
Elsa
2.23
0.372
3.63
120.0
3.62
3.2
68.00
16.67
20.27
21.93
Cu +P. f+ F.o.l
2.35
0.436
3.81
130.5
3.93
3.3
71.70
18.00
16.43
21.93
SA + P. f + F.o.l
2.26
0.430
3.87
129.8
3.76
3.3
70.30
17.00
12.23
17.70
Elsa +P. f + F.o.l
2.40
0.466
3.95
135.2
4.28
3.5
73.36
19.00
7.69
8.85
Cu +SA +P. f + F.o.l
2.19
0.422
3.85
123.3
3.89
2.8
60.60
16.33
17.71
26.56
Elsa + Cu + SA + F.o.l
2.15
0.358
3.22
112.2
2.76
2.2
52.80
13.67
36.47
39.23
P. f + F.o.l
0.68
0.082
0.68
8.78
0.86
0.87
4.85
1.77
7.76
16.28
F.o.l
Elsa
+ F.o.l
R.L.S.D (0.05)
*
27
...
:
&
-4
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Efficiency of Some Chemical and Biological Induction Compounds In The
Reduction of The Tomato Plant Infection by The Fungus
Fusarium oxysporum Schl. f.sp.lycopersici
J. T. A. AL-korany
and
M. A. Fai'adh
Plant Protection / College Agriculture / University of Basrah
Abstract
This study was aimed to evaluate the affectivity of some chemical and biological
factors (the fungicide Esla, copper, and salicylic acid) and the bacteria Pseudomonas
fluorescens pf. DS singularly or collectively in reduce infection of plant tomato by disease
Fusarium wilt disease caused by Fusarium oxysporum Schl f.sp lycopersici.The results
showed that P. fluorescens and the bacterial filtered treated and untreated with heat have a
high antagonistic ability in inhibition growth of F.o.l. in the culture where inhibition zone
reached 3,3 and 2.4 cm respectively. The results also showed that fungicide Elsa inhibition
the growth of the infections fungus F.o.l completely reaching 100% rate in all concentration
tested 10-50 ppm. Copper and salicylic acid showed no effect on the fungus growth at low
concentration , whereas high concetration inhibted the fungus growth. The treatment with ( P.
fluorescens + salicylic acid + CuSO4 H2O + F.o.l ) achieves the least infection rate in the
green house and the field. The chemical factors induce resistance and P. fluorescens cause
an increase in some parameters of the shoot growth as the dry wieght of shoot and root system
and N. P. K .
30