1. No category

What is the purpose of Bio8970?
Why do we need such a course?
What can it do for me?
What will be covered?
What will be required of me?
What is the purpose of Bio8970?
Why do we need such a course?
What can it do for me?
What will be covered?
What will be required of me?
What is the purpose of Bio8970?
Why do we need such a course?
What can it do for me?
What will be covered?
What will be required of me?
What is the purpose of Bio8970?
Why do we need such a course?
What can it do for me?
What will be covered?
What will be required of me?
Requirements ?
Attendance ! ! For PhD students................
For Masters Students...........
Requirements:
Attendance Submission of a 3-5 page (single-spaced, discounting any figures)
detailed appreciation as to how any one of the technologies/
instruments discussed in this course could be used to enhance your
own research.
Due the last day of classes
This submission should include:
an introduction (general background of the technology);
a discussion as to how and why the technology might apply
specifically to your research, together with a somewhat
detailed materials & methods section;
a bibliography, which must include at least four pertinent
publications that have used the technology being discussed.
What are the Advanced Biotechnology
Core Facilities
DNA : Protein
Core facility
Mass Spectrometry
Core facility
Combinatorial
Core facility
Cell, Protein & DNA
Core facility
Structural
Core facility
Kell Hall
Natural Science Center
Petit Life Science Center
as of.... before my time
as of June 2003
as of April 2nd, 2010
Center for
BIOTECHNOLOGY
& DRUG DESIGN
Cell, Protein and DNA Core
Facilities: Providing Equipment &
-at cost* to GSU Faculty / Staff,
Service
& GRA member institutions
ABCore Facilities
http://biology.gsu.edu/core
What are the Cell, Protein and
DNA Core Facilities?
The Cell, Protein and DNA facilities provide a multilayered network of instrumentation
and services.
What are the Cell, Protein and
DNA Core Facilities?
The Cell, Protein and DNA facilities provide a multilayered network of instrumentation
and services.
The Core facilities are designed to facilitate and enhance
both the research and the research experience of
students within the Depts of Biology and Chemistry.
As such, they provide varying levels of training and
education about the instruments
within the core
(Technical Support Level I) General Access Instrumentation
Maintenance and Training
Provide maintenance of General Access instrumentation and
general training to ALL researchers who use the Core.
(Technical Support Level II) Specialized Instrumentation
Maintenance and Training
Provide maintenance of the more Sophisticated Instrumentation
and hands-on training in the appropriate use of these specialized
technologies.
(Instruction) Specialized Instrumentation Training
Specialized instruction in the sophisticated techniques that are
used within the Core Facility
Bio8970, Bio4910 (Summer) and Bio4905 (Summer) and support
for ALL Molecular- Microbiology- based Undergraduate
laboratories in Biology, as well as “in-lab” demonstrations and
facility tours.. Bio2107 labs
FY2004"
FY2005"
FY2006"
FY2007"
Biology"
Fiscal'Year'
FY2008"
Chemistry"
Physics"
FY2009"
NI"
Animal"facility"
FY2010"
Public"Health"
outside"GSU"
FY2011"
NutriJon"
FY2012"
FY2013"
FY2014"
0"
200"
400"
600"
800"
Number'of'Researchers'Trained'by'the'Core'Staff'
1000"
1200"
http://www.gra.org
Level I
General Access Equipment
(relatively low maintenance)
from permeablizing cell membranes Gene Pulse Electroporation System (BioRad).....
..to Cell breaking -Sonifier 450
(Branson -top right)
and
French Press (Aminco)
Lyophilizer (LabConco)
Thermocyclers
(Eppendorff)
Liquid scintillation Counter LS6500
(Beckman)
Automated Protein Separation
AKTA
Explorers / Purifiers (GE)
Plate -Reader (Spectramax)
Victor X3 plate-reader
(Perkin Elmer)
Plate -Reader (Beckman)
Assorted Micro-Plate Readers
EnSpire Multimode Plate Reader
Omega UV-Imaging System
(Ultra-Lum)
Phosphoimager
(Fuji) Model Bas 2500
UVP GelDoc
Gel documentation imaging system
Fluorescent Bioimaging
System
FLA7000 (Fuji/GE)
Chemi-Luminescence Image
Analyzer
(Fuji) Model LAS-4000-mini
Optima MAX-XP
Table-top Optima model- Assorted Centrifuges
and
Ultracentrifuges-
to the Analytical XLA-X1
Level II
Decentralized Specialized Equipment
(high maintenance)
Level III
Centralized Equipment Service
and Molecular Analysis
Quantitative PCR (qPCR)
Amplification of DNA/RNA
Models ABI/Life 7300, 7700
7500 FAST & StepOne
Capillary DNA Sequencing
(ABI/ Life technologies) Model 3730
Robotic
Workstation
Biomek 2000
(Beckman)
Robotic Workstation
(Beckman) Biomek NX
Cytometry:
Automated
Cytometry
Fluorescent Flow
Viable
Cell Counter
LSR
Fortessa
(Becton Dickinson)
Cellometer
Auto2000
(Nexcelom)
Automated Flow Cytometry
FACSCanto II (Becton Dickinson)
Normal Bone
Marrow
Chronic Myelogenous
Leukemia
Acute lymphblastic
Leukemia
Chronic Lymphocytic
leukemia
Use of up to six fluorescent markers to asses the
presence of various specific types of cancer, enables
precise diagnosis.
http://bloodjournal.hematologylibrary.org/cgi/content/full/90/8/2863
Mobile Flow Cytometry
Accuri C6 (Becton Dickinson)
FACS AriaII (Becton
Dickinson)
Microscopy:
Zeiss Axio Observer Z1,
Zeiss 510 & 700
Confocal Microscopes
(Bioimaging Facility)
Deconvolution Microscope
(Deltavision)
Atomic Force Microscopy:
AFM / SFM
(Veeco/Bruker)
Multimode V III
Atomic Force Microscope (AFM) operates by measuring attractive or repulsive forces between a probe or “tip” and the sample.
The tip is located at the end of a leaf spring or “cantilever”. A laser beam is reflected off the cantilever. Any angular deflection of the
cantilever caused by the change of the force between tip and sample is represented by the angular deflection of the laser beam. Images
are taken by scanning the sample relative to the tip and measuring the deflection of the cantilever as a function of lateral position.
Different from traditional microscope, image from AFM is three dimensional.
42
Domain
-­‐ C34
+ C34
N68
N69
N70D
N72
SecA
Genomics:
Microarray Technology: enables us to monitor variable mRNA
expression -at the level of genomic RNA (transcriptome).
Genomic Analysis
Design
Experiment
Prepare
Sample
Probe
Array
Hybridize
Hybridization
Oven
Wash & Stain
Scan
Analysis
Fluidics Station
Scanner
Analysis
Software
Capillary DNA Sequencing
(ABI/ Life technologies) Model 3730
454 -GS Junior Next-Gen, Deep Sequencing
(Roche)
Ion Proton Next-Gen, Deep Sequencing
(ABI/Life technologies)
Proteomics:
Protein Analysis:
Western Blotting
Proteomics II:
Ettan II 2D-gel Proteomics
System (GE Lifesciences)
-complemented by a
MALDI TOF/TOF(ABI) Model 4800+
Proteomics:
Text
http://biology.gsu.edu/core
Text
http://biology.gsu.edu/core
http://biology.gsu.edu/core