De Boelelaan 1081 1081 HV Amsterdam The Netherlands T. +31 (0) 20 59 87 984 [email protected] www.LUMICKS.com Acoustic Force Spectroscopy (AFS™) Acoustic Force Spectroscopy (AFS™) Product description Acoustic Force Spectroscopy, or AFS™ is a single-molecule, lab-on-a-chip technology for highly-parallel single-molecule manipulation and measurements. In AFS™ acoustic waves (ultrasounds) are employed to exert forces from sub-pN to hundreds of pNs on thousands of biomolecules (such as DNA, RNA or proteins) General overview •Lab-on-a-chip • Single-molecule manipulation • Massively parallel • Compatible with inverted microscopes • Safe and user-friendly •Cost-effective in parallel, with sub-millisecond response time and cell mechanics. In addition, free energy and inherent stability. AFS™ represents an ideal diagrams, kinetic rates and intermediate tool to perform a wide variety of experiments and states in reaction pathways can also be decipher the details of molecular interactions at studied and identified. AFS™ is integrated the level of nucleic acids-proteins, drug-protein in a small and compact microfluidic device, and antigen-antibody. AFS enables scientist to providing an easy-to use, cost-effective investigate structure-function of proteins, new instrument that can be easily implemented biological mechanisms, compound screening on any custom or commercial inverted microscope using bright field illumination. AFS™ technology consists of a glass microfluidic chip and includes transparent piezoelectric transducers that generate acoustic waves. The contact wires are directly deposited on the AFS™ device and a reliable electric and fluidic connection is insured using the AFS™ chip-holder. AFS™ is a versatile instrument to perform cutting-edge biological research at the single-molecule level. Capture molecular interactions with AFS™. Technical overview Real-time parallel tracking in 3D (x,y,z) 100 microspheres at 60 Hz Real-time parallel tracking in 2D (x,y) 2000 microspheres at 60 Hz Real-time position tracking accuracy (x,y) 2 nm at 60 Hz Real-time height tracking accuracy (z) 3 nm at 60 Hz Minimum force incremental step 2 femtoNewtons Maximum force applicable 600 pN using 4.5 μm polystyrene beads Range of loading rates 10-4 pN/s to 103 pN/s (limited by polymer physics) AFS™ chip size (configurable) 45 mm x 15 mm x 1.275 mm Applications • Dynamic force spectroscopy • Constant-force measurements • Force-distance curves • Biopolymer mechanics •Micro-rheology AFS™ lab-on-a-chip Sitters, G. et al. Acoustic Force Spectroscopy. Nature Methods 12, 47–50 (2015). • Cell mechanics • Protein folding dynamics • DNA/RNA protein interactions
© Copyright 2024