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Acoustic Force Spectroscopy (AFS™)
Acoustic Force Spectroscopy (AFS™)
Product description
Acoustic Force Spectroscopy, or AFS™ is a
single-molecule, lab-on-a-chip technology for
highly-parallel single-molecule manipulation
and measurements. In AFS™ acoustic waves
(ultrasounds) are employed to exert forces from
sub-pN to hundreds of pNs on thousands of
biomolecules (such as DNA, RNA or proteins)
General overview
•Lab-on-a-chip
•
Single-molecule manipulation
•
Massively parallel
•
Compatible with inverted microscopes
•
Safe and user-friendly
•Cost-effective
in parallel, with sub-millisecond response time
and cell mechanics. In addition, free energy
and inherent stability. AFS™ represents an ideal
diagrams, kinetic rates and intermediate
tool to perform a wide variety of experiments and
states in reaction pathways can also be
decipher the details of molecular interactions at
studied and identified. AFS™ is integrated
the level of nucleic acids-proteins, drug-protein
in a small and compact microfluidic device,
and antigen-antibody. AFS enables scientist to
providing an easy-to use, cost-effective
investigate structure-function of proteins, new
instrument that can be easily implemented
biological mechanisms, compound screening
on any custom or commercial inverted
microscope using bright field illumination. AFS™ technology consists of a glass microfluidic chip
and includes transparent piezoelectric transducers that generate acoustic waves. The contact
wires are directly deposited on the AFS™ device and a reliable electric and fluidic connection
is insured using the AFS™ chip-holder. AFS™ is a versatile instrument to perform cutting-edge
biological research at the single-molecule level. Capture molecular interactions with AFS™.
Technical overview
Real-time parallel tracking in 3D (x,y,z)
100 microspheres at 60 Hz
Real-time parallel tracking in 2D (x,y)
2000 microspheres at 60 Hz
Real-time position tracking accuracy (x,y)
2 nm at 60 Hz
Real-time height tracking accuracy (z)
3 nm at 60 Hz
Minimum force incremental step
2 femtoNewtons
Maximum force applicable
600 pN using 4.5 μm polystyrene beads
Range of loading rates
10-4 pN/s to 103 pN/s (limited by polymer physics)
AFS™ chip size (configurable)
45 mm x 15 mm x 1.275 mm
Applications
•
Dynamic force spectroscopy
•
Constant-force measurements
•
Force-distance curves
•
Biopolymer mechanics
•Micro-rheology
AFS™ lab-on-a-chip
Sitters, G. et al. Acoustic Force Spectroscopy. Nature Methods 12, 47–50 (2015).
•
Cell mechanics
•
Protein folding dynamics
•
DNA/RNA protein interactions