How to Read the Chromatin Past Vincenzo Pirrotta Science 337, 919 (2012); DOI: 10.1126/science.1227684 If you wish to distribute this article to others, you can order high-quality copies for your colleagues, clients, or customers by clicking here. Permission to republish or repurpose articles or portions of articles can be obtained by following the guidelines here. The following resources related to this article are available online at www.sciencemag.org (this information is current as of September 2, 2012 ): Updated information and services, including high-resolution figures, can be found in the online version of this article at: http://www.sciencemag.org/content/337/6097/919.full.html A list of selected additional articles on the Science Web sites related to this article can be found at: http://www.sciencemag.org/content/337/6097/919.full.html#related This article cites 8 articles, 4 of which can be accessed free: http://www.sciencemag.org/content/337/6097/919.full.html#ref-list-1 This article appears in the following subject collections: Molecular Biology http://www.sciencemag.org/cgi/collection/molec_biol Science (print ISSN 0036-8075; online ISSN 1095-9203) is published weekly, except the last week in December, by the American Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005. Copyright 2012 by the American Association for the Advancement of Science; all rights reserved. The title Science is a registered trademark of AAAS. Downloaded from www.sciencemag.org on September 2, 2012 This copy is for your personal, non-commercial use only. Earth system “resilience,” would be viewed as an emergent and evolving property of the climate system, rather than as a constant. The long-standing mystery of the late Pleistocene ice ages illustrates how a constant/universal linearization of global temperature versus radiative forcing can provide an overly narrow view of climate-adjustment processes. These climate cycles were clearly paced by a slowly evolving insolation field that did not produce significant global average radiative anomalies (at least under the usual assumption of a globally uniform response time). Crucially, this insolation forcing was only effective because of strong radiative feedbacks, including changes in atmospheric CO2 levels. The emergence of these feedbacks appears to have been strongly conditional on the prevailing climate state (11) and may have also depended on the occurrence of abrupt (“irreversible”) transitions in regional climate and the ocean circulation (12). The tiered time and space scales involved in these global climate upheavals underline the importance, for understanding and predicting long-term climate change, of considering the full spectrum of response times in the climate system and their interaction. An exclusive consideration of the highest (e.g., decadal) register of climate variability might be adequate for most political time frames and may suit the urgency of immediate mitigation and adaptation challenges. However, it falls short of the wider scientific challenge that faces humanity, as well as a moral horizon that extends much farther into the future. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. References and Notes 1. 2. 3. 4. R. Knutti, G. C. Hegerl, Nat. Geosci. 1, 735 (2008). S. Arrhenius, Philos. Mag. J. Sci. 41, 237 (1896). G. S. Callendar, Q. J. R. Meteorol. Soc. 64, 223 (1938). S. Solomon et al., Eds., Climate Change 2007: The Physical Science Basis. Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change (Cambridge Univ. Press, Cambridge and New York, 2007). 18. J. Hansen et al., Open Atmos. Sci. J. 2, 217 (2008). M. Crucifix, Geophys. Res. Lett. 33, L18701 (2006). E. N. Lorenz, Quat. Res. 6, 495 (1976). C. Wissel, Oecologia 65, 101 (1984). Tipping points need not be globally catastrophic. A longterm irreversible transition can be composed of a series of spatially or temporally local tipping points (e.g., in land cover, ice-sheet stability, or ocean circulation). The irreversibility of such transitions is not absolute, but defined relative to the duration of the triggering perturbation. I. M. Held, Bull. Am. Meteorol. Soc. 86, 1609 (2005). D. Paillard, Rev. Geophys. 39, 325 (2001). G. H. Denton et al., Science 328, 1652 (2010). J. A. Higgins, D. P. Schrag, Earth Planet. Sci. Lett. 245, 523 (2006). M. I. Hoffert, C. Covey, Nature 360, 573 (1992). P. Köhler et al., Quat. Sci. Rev. 29, 129 (2010). M. Pagani et al., Science 314, 1556 (2006). M. Pagani, Z. Liu, J. LaRiviere, A. C. Ravelo, Nat. Geosci. 3, 27 (2010). J. Rogelj, M. Meinhausen, R. Knutti, Nature Clim. Change 2, 248 (2012). Supplementary Materials www.sciencemag.org/cgi/content/full/337/6097/917/DC1 Table S1 10.1126/science.1224011 MOLECULAR BIOLOGY How to Read the Chromatin Past Several mechanisms are used by cells to maintain specific histone modifications and gene activity through successive cell divisions. Vincenzo Pirrotta I t has become common parlance to refer to all histone modifications as “epigenetic,” meaning that they carry information about how to use the associated DNA sequences as a function of earlier events. However, some histone modifications, such as methylation of histone H3 at lysine 27 (H3K27), are more epigenetic than others in that they can self-renew from one cell cycle to the next, thereby establishing a “cellular memory” of earlier events. A series of articles in the past 3 years—including one by Yuan et al. (1) on page 971 of this issue—has revealed several subtle mechanisms by which the Polycomb repressive complex 2 (PRC2), which methylates H3K27, reads the preexisting chromatin state to ensure that it is faithfully maintained after cell division (2–7). PRC2 is one of two complexes involved in establishing and maintaining the Polycomb-repressed chromatin state [for a review, see (8)]. It produces H3K27 trimethylation (H3K27me3), the characteristic mark associated with Polycomb repression. H3K27me3 stabilizes the binding of the PRC1 and directs its action to the target gene. Both PRC comMolecular Biology and Biochemistry, Rutgers University, 604 Allison Road, Piscataway, NJ 08854, USA. E-mail: [email protected] plexes are recruited to target genes by various mechanisms ranging from DNA-binding factors at Polycomb response elements to long noncoding RNAs (1). Both complexes receive stabilizing inputs by the presence of H3K27me3: PRC1 through the presence of the Polycomb protein with its H3K27me3binding chromodomain and PRC2 through recognition of H3K27me3 by its ESC component (mammalian Eed) (2). PRC2 has the job of recognizing the previous state of the chromatin and maintaining it after cell division. Two examples clarify what is involved. One is the classical maintenance of a Polycomb-repressed state. Although genes can switch from a repressed to an active state in response to specific activators, Polycomb-repressed genes tend to stay repressed from one cell cycle to the next. This implies that the PRC2 complex can sense what regions contain H3K27me3 from the previous round. The other example is one in which a gene adjacent to a Polycomb-silenced gene is in the active state. What prevents Polycomb silencing from spreading into the adjacent active gene and silencing it as well? One answer might be that the active chromatin state antagonizes the establishment of the repressed state. This behavior is well known in the Drosophila early embryo where, when Polycomb repression becomes functional, it silences Hox genes where they are inactive but does not interfere with them when they are actively transcribed. To understand how PRC2 recognizes and maintains both repressive and active chromatin states, one must consider the components and properties of the PRC2 complex. Although the methyltransferase is the E(Z) protein (mammalian Ezh1 and Ezh2) with its SET domain as the catalytic center, E(Z) by itself has no activity. It needs to be associated at least with the ESC/EED and SU(Z)12/Suz12 components and, for optimal activity, with the histone chaperone variously called NURF55/CAF1/RbAp48 because of its multiple roles (1). These three noncatalytic components position the complex on the nucleosome, read input signals, and allosterically modulate the methyltransferase activity of E(Z). At least three PRC2 core components are involved in binding to a nucleosome and identifying histone H3 as the target. The NURF55 component binds to the N-terminal tail of histone H3 protruding from the nucleosome, helping to position the complex on its substrate (6). The N-terminal tail of histone H3 is the site of numerous possible modifications. One of these, H3K4me3, is associated with www.sciencemag.org SCIENCE VOL 337 24 AUGUST 2012 Published by AAAS 919 Downloaded from www.sciencemag.org on September 2, 2012 PERSPECTIVES PERSPECTIVES 920 H3 a.a. 35–42 H3K27me2, me3 Stimulate NURF55 SU(Z)12 VEFS E(Z) ESC PRC2 SET H3K4me3 H3K27me3 H3K36me2, me3 Inhibit Feedback and feed-forward on PRC2 activity. The structure of PRC2 is illustrated schematically. It is not known whether any of the four core components— NURF55, SU(Z)12, E(Z), and ESC—are present in more than one copy. E(Z) (mammalian Ezh2) is the enzymatically active methyltransferase. When it acts on a target nucleosome (bottom), it receives inhibitory signals from methylation (me) states at histone H3 lysine 4 (K4) and lysine 36 (K36). It also receives powerful activating inputs from neighboring nucleosomes (top), again from H3 region 35–42 and from the presence of methylation at lysine 27 (K27). a.a., amino acids. because transcriptional activity and its associated features destabilize nucleosomes. The work of Yuan et al. (1) shows that, when nucleosomes are tightly packed, the PRC2 complex bound to a target nucleosome senses a neighboring nucleosome by recognizing its histone H3. Again, this triggers a conformational shift that is transmitted to the catalytic domain of E(Z), resulting in much higher methylating activity on the target nucleosome. The key is the SU(Z)12 component, which binds amino acids 35 to 42 of histone H3. Adding the H3 35–42 peptide to a reaction suffices to stimulate the methylation activity on a low-density nucleosome target. The SU(Z)12 region that binds the peptide is once again the VEFS region that interacts with E(Z), and the fact that the 35–42 peptide includes lysine36 strongly suggests that this is also the region that senses the lysine 36 methylation status. There are, to be sure, many difficulties to be resolved in these emerging pictures of PRC2. One is how the same VEFS domain of SU(Z)12 is able to interact with H3K4 and H3K36 on the same H3 molecule that is methylated by E(Z) and at the same time sense the nucleosome density by interacting with another nucleosome, all while interacting with E(Z). This seems like a contorsionist’s nightmare although, very likely, PRC2 contains two copies of some or all of its components. Another fascinating structural question is how the various inputs are transmitted to E(Z) and converted into independent allosteric rearrangements that alter the catalytic activity of its SET domain. Understanding this will probably require solving the structure of the interfaces between E(Z) and SU(Z)12 and ESC. There remains a nagging question in these and other studies on PRC2 function. What PRC2 function is chiefly targeted by these various mechanisms? The general assumption is that it is the function associated with Polycomb repression of specific target genes. This is the best-known function of PRC2 and clearly benefits from a self-renewal mechanism. A much less well understood role of PRC2 is the genome-wide H3K27 dimethylation that it appears to produce by a hit-andrun mechanism. The result is to dimethylate H3K27 at all sites except those that contain H3K27me3 and those that are transcriptionally active. It is this function that might otherwise operate in transcribed regions and that is inhibited by the negative effects of H3K4 and H3K26 methylation and of low nucleosome density. Remarkably, this global function of PRC2 would mean that the feedback and feed-forward mechanisms can provide not only the self-renewal of the repressive H3K27me3 mark but also a memory of a region’s transcriptional activity since the loss of H3K27 dimethylation would tend to persist in the next cell cycle and favor another round of transcriptional activity. More work is needed to understand this role of PRC2. Meanwhile, PRC2 provides a clear example of the epigenetic nature of at least some histone modifications. References 1. 2. 3. 4. 5. 6. 7. 8. W. Yuan et al., Science 337, 971 (2012). R. Margueron, D. Reinberg, Nature 469, 343 (2011). K. H. Hansen et al., Nat. Cell Biol. 10, 1291 (2008). C. Xu et al., Proc. Natl. Acad. Sci. U.S.A. 107, 19266 (2010). W. Yuan et al., J. Biol. Chem. 286, 7983 (2011). F. W. Schmitges et al., Mol. Cell 42, 330 (2011). R. Margueron et al., Nature 461, 762 (2009). C. S. Ketel et al., Mol. Cell. Biol. 25, 6857 (2005). 24 AUGUST 2012 VOL 337 SCIENCE www.sciencemag.org Published by AAAS Downloaded from www.sciencemag.org on September 2, 2012 Nucleosomes 10.1126/science.1227684 CREDIT: P. HUEY/SCIENCE the promoter region of transcriptionally active genes. H3K4me3 interferes with the binding of NURF55. This is not a major loss because SU(Z)12 also binds to histone H3. Even in the absence of NURF55, a domain near the C-terminal region of SU(Z)12 called VEFS binds to the N-terminal region of H3 (6). VEFS is also the interface that interacts with E(Z) (1). When the lysine 4 of histone H3 is trimethylated, it causes a conformational change that is transmitted to E(Z) and affects its catalytic domain, called the SET domain, greatly reducing its ability to release the H3 substrate. Thus, H3K4me3 present on active genes prevents turnover and reduces the methylation activity of PRC2 (see the figure). SU(Z)12 recognizes another modification of histone H3 that is associated with transcriptionally active genes: methylation of lysine 36 (5, 6). When the target histone H3 is di- or trimethylated at lysine 36, the effect is again transmitted to the catalytic domain of E(Z) and its methylation activity is reduced. As a consequence, H3K4me3 and H3K36me2/me3 strongly inhibit PRC2 methylation of H3 lysine 27 in genes that have been recently transcribed. Other signals tell PRC2 where to be particularly active (see the figure). One is the prior presence of H3K27 methylation in a chromatin neighborhood. Structural studies of the ESC/EED component revealed an aromatic pocket formed by contributions from different parts of the protein (2). This aromatic pocket binds methylated lysines and, in particular, binding of histone H3 di- or trimethylated at lysine 27 triggers a conformational change that is somehow transmitted to E(Z), strongly enhancing its catalytic activity. Unlike the H3K4me3 and H3K36me2/me3 marks, which have to be on the same histone H3 to be methylated, the H3K27me3 mark must be on a separate molecule. Consequently, chromatin regions that already contain H3K27 methylation are preferential targets for new methylation. This is the situation that prevails when a gene repressed by Polycomb is replicated: the old methylated histones are partitioned between the two daughter chromatin strands, and new, unmethylated histones are deposited to restore the full nucleosome complement. To ensure the maintenance of the H3K27 methyl mark, these new nucleosomes must be preferentially targeted by PRC2. This is ensured by the presence of neighboring nucleosomes already bearing H3K27 methylation. Another device that helps to the same end does not rely on histone modifications but on nucleosome density. Transcriptionally active chromatin has a lower nucleosome density because the linker histone H1 is absent and
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