How it works
Estrid Høgdall
Director of Danish CancerBiobank
Head of Molecular Unit, Department of Pathology, Herlev Hospital
Danish CancerBiobank
Why establish Dansk CancerBiobank?
•
A wish to collect biological materials, handled, stored and registered
optimally for translational reseach
•
A wish to establish a nationwide research biobank containing cells,
tissue, blood, salvia, hair etc.
•
A wish to complement the material in Patobanken (composing paraffin
embedded tissues) with fresh frosen tissues
And thereby
Implement, support and optimize the infra-structure
for clinical cancer research
Organization of Danish CancerBiobank
National Steering Committee
Settled by Danish Regions
Meets 2 × årligt or when needed
Regional Health Directors
Faglig følgegruppe (Scientific Board):
Meets 2 × årligt or when needed
National Steering
Committee
National
Secretry
Scientific Board
External partners
(Faglig følgegruppe)
Biobankcenter
Center
Departments
Local
departments
Herlev
Klinisk
Biokemisk
Afdeling
Patologiafdelingen
Næstved
Klinisk
Biokemisk
Afdeling
Klinisk
Patologisk
Afdeling
Teknik
Patientinformation og
hjemmeside
Anvendelse og håndteringsomkostninger
Kontrakter
Dataregistrering
Kvalitetsstyring
Odense
Afd. for
Klinisk
Biokemi
Afd. for
Klinisk
Patologi
Rigshospitalet
Klinisk
Immunol.
Afdeling
Patologiafdelingen
Aalborg
Klinisk
Biokemi
Patologisk
Institut
Århus
Molekylær
Medicinsk
Afdeling
Patologisk
Institut
Infra-structure
Dansk CancerBiobank
Nationwide
On-line registration in centralized database
Secretry at Molecular Unit, Dept. of
Pathology, Herlev Hospital, University of
Copenhagen
Danish
Cancer
Biobank
DATA
Hospitals collect samples
→
High quality
- Standardisation
- Trained employe
- High coverage
- Low cost
--
Sample flow - Collaboration
Blood samples for
Danish CancerBiobank
Tissue samples for
Danish CancerBiobank
Max 14 days
Requisition
First visit
Biopsy/Surgery
Post treatment – from
January 2013
Accept
Clinical databases and other sources
for clinical informations
National registries
National guidelines
Tissue fractions
Blood fractions
9-10 mm
3 mm
3 mm
5 mm
Alternativ udskæring
3 mm
EDTA -blod
Blodet tappes
Væv til formalin/paraffin
Blod i gelglas
5 mm
Tissue-Tek behandling
Frisk frys Væv til RNA-later og Tissue-Te
5
Fuldblod afpipetteres
HE - side 2
1 x 1½ml fuldblod
Centrifugering
6
7
2 x 2ml plasma
8
Tissue -Tek og RNA
3x10x10 udsk æres til
Centrifugering
Hvidt mellemlag
(buffy coat)
1 x 1½ml buffycoat
Formalin/paraffin
3x10x10 mm (5)
Frisk frys
3x10x10 udsk æres til
4 stk væv 5x5x3 mm
2 stk væv 5x5x3 mm
2 stk væv 5x5x3 mm
1
2
3
4
Tissue -Tek
6
7
1
2
3
4
Side 2 Side 1
4 x 2ml serum
RNA
Priority order:
Whole blood
Serum
EDTA-plasma
Buffy coat
HE - side 1
miRNA
DNA
RNA
Urine
…
8
9
Priority order:
Fresh frozen
FFPE
O.C.T. Tissue-Tek
RNAlater
Estrid Høgdall
Vital tumor
Necrosis
Pneumonia
Normal tissue
Small Cell Lung Carcinoma
Infra-structure of Danish CancerBiobank
Before surgery
Surgery
Departments of Surgery
Clinical Departments/Outpatients clinics
Blood samples
Tumor tissues
Biobank data
Biobank data
Danish CancerBiobank - data
National biobank web-modul
for registration of data
Data
Patologi system
Patient-data
Patobank
Diagnostic samples
Biobank samples
Diagnostic samples
FFPE/fresh
Biobank samples
Danish CancerBiobank – blood
Danish CancerBiobank - tissue
Central/local
Handling/freezing/storage
Whole blood/serum/plasma/buffy coat
Central/local
Handling/freezing/storage
Fresh frozen/FFPE/Tissue -Tek/RNAlater
Registration: blood
BioBank Use case
Registrer blod
WebService
CPR-opl.
Registrer
patientdata
Proceder blod til
BioBank
Patient
NOTE
Lokale og centrale frysere ligger
fysisk i samme
database
Udtag blod
Registrer
rekv. data
Bio Analytiker
Tildel unikt
løbenummer
Fryser
(Lokal)
Label
Blod prøver
Registrer
adm. data
La
be
l sæ
Fryser
(Central)
t
Udskriv label
ke
ifik
ec ls
Sp labe
Registrer
blod data
Skift status
Vælg fryser
placering
BIOBANK FLOW
1. Registrer patientdata
2. Registrer administrative data
3. Registrer blod data
4. Udskriv label
5. Påsæt label
6. Vælg fryser (i)
7. Find ledig plads i fryser (i)
8. Vælg plads i fryser (i)
9. Placer i fryser
(i) Iterativ process
BioBank
Placer i skab/
fryser
Kilde: logica
Estrid Høgdall
Registration: tissue
BioBank Use case
Registrer væv
WebService
CPR-opl.
Bio Analytiker
Udtag væv
Registrer
patientdata
Proceder væv til
BioBank
Patient
Kliniker
i
Væv
Registrer
rekv. data
Fremsend til
Patologisk afd.
Rekvisition
er
bøtt
NOTE
Lokale og centrale frysere ligger
fysisk i samme
database
Label
Væv
Fryser
(Lokal)
WebService
Pato. opl.
Registrer patologi rekvisition
Registrer
adm. data
Labelsæt
Udskriv label
Fryser
(Central)
ke
ifik
ec ls
Sp labe
Patolog
BIOBANK FLOW
1. Registrer patientdata
2. Registrer adm. data
3. Registrer vævs data
4. Udskriv label
5. Påsæt label
6. Vælg fryser (i)
7. Find ledig plads i fryser (i)
8. Vælg plads i fryser (i)
9. Placer i fryser
(i) Iterativ process
NOTE
Overførelse af
rekvisitionsdata
fra det lokale
Patologisystem
til PatoBank
foregår asynkront
og der er derfor
en tidsforsinkelse
på nogle minutter
Registrer
vævsdata
PatoBank
Vælg fryser
placering
Patologi
PatoBank
BioBank
Skift status
Placer i skab/
fryser
Kilde: logica
Estrid Høgdall
On-line registration system
12
Classifications in the registration
system
Simple
Type of material
Temperature
Tubes
Centres
Verification of diagnosis
Complexe
Projects
Freezers
Connect samples to the research projects
4 National projects
29 Local projects
Registation of materials – detail informations
16
Label on stored material
Send material
Receive material
Export of biobank data for research
Excell
Reports
Import of historical data
Kilde: logica
Estrid Høgdall
Unique CPR
Aalborg
Herlev
Næstved
Odense
Rigshospitalet
Århus
All
Blood
Tissue
Blood
Tissue
Blood
Tissue
Blood
Tissue
Blood
Tissue
Blood
Tissue
Blood
Tissue
2010
2011
2012
285
160
1517
642
93
48
146
748
656
582
993
187
440
231
1092
849
477
354
661
1022
750
1392
1157
424
641
441
2891
839
484
395
726
1175
1177
1130
1440
540
3690
2367
4577
4272
7359
4520
3500
3000
2500
2000
2010
1500
2011
1000
2012
500
0
Blood Tissue Blood
Aalborg
Tissue Blood
Herlev
Tissue Blood
Næstved
Tissue Blood
Odense
Tissue Blood
Rigshospitalet
Tissue
Århus
Samples in DCB
Unique CPR with biological material
Tissues samples
Blood samples
Corresponding blood and tissue samples
Registered without tissue
2010
5293
3369
3894
781
579
2011 2012
7082 9609
6288 6814
6098 11121
2067 3056
984 1682
12000
10000
8000
2010
6000
2011
2012
4000
2000
0
Unique CPR with
biological material
Tissues samples
Blood samples
Corresponding blood and Registered without tissue
tissue samples
Research studies in DCB
Research projects
2010
2011
2012*
National
4
4
4
Local**
17
28
31
•*1.3.2013
** 1 study local at to departments
35
30
25
20
National
Local**
15
10
5
0
2010
2011
2012*
Blood and tissue fractions marked for
research projects
2010 2011 2012
Tissue fractions
1091 3913 4679
Blood fractions
7313 12486 21004
Total no. of fractions marked for research 8844 16688 28944
30000
25000
20000
Tissue fractions
15000
Blood fractions
10000
Total no. of fractions marked for
research
5000
Total no. of fractions marked…
Blood fractions
Tissue fractions
0
2010
2011
2012
Progress
Material in Danish CancerBiobank from 2010 to 2012
(pr. year, unique CPR)
8000
7000
6000
5000
Blod
4000
Væv
3000
2000
1000
0
2010
2011
2012
Increase in registration/collection of blood and tissue
in Danish CancerBiobank (2010 to 2012)
Increase from 2010 til 2012
Blood
Tissue
50%
52%
Anvendelse af data og biologisk materiale fra Dansk CancerBiobank
Anmodning f ra f orskere til sekretariat for DCB web-modul v edrørende
data (antal, ty pe, centertilkny tning, projekttilknytning etc ) – gratis
Kontakt til relev ante centre/projekter om samarbejde og ev t. kontakt til DMCG eller andre kilder til kliniske data
N ej
Skriftligt afslag indeholdende
begrundelse f or af gørelsen
Ja
Tillade lse f ra Videnskabs Etisk Komite, Datatilsy net, relev ante biobanksudv alg og evt. DMCG eller andre
kilder til kliniske data samt tilladelse f or evt. projektreserv eret materiale
Samarbejdet etableres og udmøntesOmkostninger forb und et med udl everi ng beta les af proj ekt efter nationa l prisl iste
* Ved rent regionale projekter kan data også indhentes fra det/d e regionale centre
http://www.cancerbiobank.dk
Easy communication
2010: 2 articles, 2011: 8 articles
Motivation for partners
-No need to worry about
sample colletion!
-Optimized treatment for
the patients in the future
-Denmark on the
map!
-Translational cancer
research!
...and the material can be
used for my research!
Patients
Doctors/researcher
Danish Regions
Perspectives for
Danish CancerBiobank
Benefits of Nationwide Biobanks
The researcher only have to contact one place to receive informations
about the collected materials, about relevant persons to be contacted
and about physical storage of the samples
Already used material can be used in other research project
Nationwide guidelines accepted for handling of the samples and
on-line registration
Quality assessment of the biological material
Corresponding informations of blood and tissues and other materials
ex. blood and tissues, DNA, RNA, urine etc
One homepage
Biobanks
- the future has begun
Thank you for your attention
Teamwork – thanks to
Ministry of Health
National Board of Health
Danish Regions
Steering Commitee
Hospital Directors
Scientific Board
Researchers
Head of Departments at the hospitals
Project Leaders at the Pathology and Clinical Biochemestry Departments
Employees at the Departments
Patients