1st Meeting of the Core Facility We are honoured that this meeting is included in the events to celebrate the 50th anniversary of the foundation of the Medical Faculty Mannheim. About the Core Facility The core facility LIMA was founded in 2011 with the goal of giving researchers and clinicians access to a powerful and innovative bio-imaging tool for the study of biological processes in action. The core facility LIMA provides dynamic imaging equipment i.e. state of the art laser scanning microscopes along with technical expertise and is currently collaborating on studies that include angiogenesis, tumour metastasis, cardiovascular physiology, cellular signalling, nociception of acute and chronic pain as well as neuronal polarity. Venue The meeting will be held at the auditorium of the Medical Faculty Mannheim, Hörsaal H02, Alte Brauerei, Röntgenstr. 7, 68169 Mannheim on the 7th of November 2014, starting at 9 o’clock with the welcoming address of the Dean. 7. November 2014 The campus is easily accessible by public transport. Fiftieth Anniversary of the Med Mannheim Meeting 1st Mannheimthe Core F of the Core Facility of LIMA “LIMA – Live Cell Imag at the CB Entrance to the venue is through the courtyard. 1st Contact LIMA: J. Bucher and W. Greffrath, CBTM, LudolfKrehl-Strasse 13-17, Tridomus C In celebration of the 50th Anniversary Medical Faculty Mannheim, Uni th of the foundation of theNovember 7 2014, 9 Lecture Room 02, „Alte Braue Medical Faculty Mannheim Meeting secretary: R. Läufer [email protected] 9.00 – 9.15 Organisation: J. Bucher, W. Greffrath and R. Läufer Text and Layout: S. Gorbey “Welcome address” by the Dean of University of Heidelberg, Prof. Dr. r Introduction by the Director of the C Neurobiology Bio-imaging tools hold enormous potential for a wide variety of diagnostics and therapeutic applications and the core facility is very proud to have established a fruitful interaction with clinicians of the Medical Faculty. Since 2014 the core facility is a member of the DGF research infrastructure portal “RIsources”, which gives researchers in Germany the possibility to use the service. Stem cells by Karen Bieback The mission of the core facility is to train and teach researchers and clinicians interested in studying biological processes in living cells or organisms. In addition the core facility also develops and refines new methods and applications, which are made available to the members of the CBTM and researchers of the Medical Faculty Mannheim. 9.15 – 9.30 Julia Bucher, CBTM, Core Facility L The Core Facility „LIMA - Live Ce Who we are and what we do 9.30 – 10.00 Hans-Ulrich Dodt, Technical Univer Ultramicroscopy of cleared mous 10.00 – 10.15 Matthias Carl, CBTM, Boutros grou Following axons in the asymmet 10.15 – 10.45 Reinhard Köster, Technical Univers Probing cerebellar function with 10.45 – 11.15 Coffee Break (catering at the Old B Vascular Biology I 11.15 – 11.45 Arndt Siekmann, Max-Planck-Institu Illuminating blood vessel formati 11.45 – 12.00 Andreas Fischer, CBTM and DKFZ Multiple PDZ domain protein con 12.00 – 12.30 Hellmut Augustin, CBTM and DKFZ Imaging technology to observe the natural course of biology in action, within living organisms. Program 9.00 – 9.15 “Welcoming address” by the Dean of the Medical Faculty Mannheim, University of Heidelberg, Prof. Dr. rer. nat. Dr. med. Dr. h. c. Uwe Bicker and introduction by the Director of the CBTM, Prof. Dr. med. Rolf-Detlef Treede Neurobiology 14.00 – 14.30 Two-and three-dimensional real time live confocal imaging of microvascular networks in situ: topology, morphology Ca2+ signaling and tone. Theodor Burdyga, University of Liverpool, Great Britain 14.30 – 14.45 BK channels transform phasic into tonic smooth muscle. Rudolf Schubert, CBTM Cellular Biology Chair: WOLFGANG GREFFRATH Chair: MICHAEL BOUTROS 9.15 – 9.30 The Core Facility „LIMA - Live Cell Imaging Mannheim“ : Who we are and what we do. 14.45 – 15.15 Systems Microscopy - using high throughput microscopy to study cellular protein dynamics for systems biology applications. Julia Bucher, CBTM, Core Facility LIMA and Treede group Drosophila oogenesis by Veit Riechmann Vascular Biology II Chair: CHRISTIAN SCHULTZ Morning Session 9.30 – 10.00 Ultramicroscopy of cleared mouse brains and mouse embryos. Hans-Ulrich Dodt, Technical University of Vienna, Austria Rainer Pepperkok, EMBL, Heidelberg 15.15 – 15.30 Control of epithelial morphogenesis via regulated endocytosis. 10.00 – 10.15 Following axons in the asymmetric brain. Matthias Carl, CBTM, Boutros group Veit Riechmann, CBTM, Boutros group 10.15 – 10.45 Probing cerebellar function with light. 15.30 – 16.00 Coffee Break Reinhard Köster, Technical University of Braunschweig 10.45 – 11.15 Coffee Break Vascular Biology I Chair: JENS KROLL Rat skin: TrpV1: red, PGP: green, Collagen: SHG by Julia Bucher Afternoon Session 11.15 – 11.45 Illuminating blood vessel formation in the zebrafish. Arndt Siekmann, Max-Planck-Institute for Molecular Biomedicine, Münster 11.45 – 12.00 Multiple PDZ domain protein controls vascular permeability. Andreas Fischer, CBTM and DKFZ, Heidelberg, Augustin group 12.00 – 12.30 Vascular control of health and disease: Seeing is believing. Hellmut Augustin, CBTM and DKFZ Heidelberg, SFB/Transregio 23 12.30 – 14.00 Lunch Break Catering at the Old Brewery 16.00 – 16.15 Cord blood-derived endothelial progenitor cells emerge from a hematopoietic CD45+ population: Multi-photon imaging to complement flow cytometric data. Karen Bieback, CBTM, Klüter group Technical perspectives Chair: JONATHAN SLEEMAN 16.15 – 16.30 The role of ASAP1 in tumor metastasis. Supriya Saraswati, CBTM, Sleeman group 16.30 – 17.00 Fluorescence Microscopy at the Nanoscale. Christoph Cremer, Institute for Molecular Biology gGmbH, Mainz 17.00 – 17.15 High-resolution microscopy of the axon initial segment and cisternal organelle in retinal ganglion cells. Maren Engelhardt, CBTM, Schultz group 17.15 – 17.30 General discussion, summary and ”fare-well“ by the Executive Leader of the Core Facility LIMA, Wolfgang Greffrath
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