Basic Laboratory Safety
November 9 – 16, 2015 – Final schedule
Campus Solna
Day 1. Monday, November 9th
Lecture hall: Wretlind, Widerströmska Huset , Tomtebodavägen 18A
Group rooms: Group room 1, 2, 3 and Ruth, Widerströmska Huset, Tomtebodavägen 18A
09:00
Introduction
Ingela Djupedal, Security and Environment Unit
Roland Möllby, MTC
09:15
Lecture: Introduction to laboratory safety including rules and regulations *
Roland Möllby, MTC
10:20
Coffee
10:40
Lecture: Introduction to risk assessments and handling instructions *
Jenny Karlsson, Security and Environment Unit
11:05
Break
11:15
Group discussion I: Own experience of safety issues in laboratory work *
Group 1: Group room 1 – Carina Bengtsson, MTC
Group 2: Group room 2 – Ingela Djupedal, Security and Environment Unit
Group 3: Group room 3 – Jenny Karlsson, Security and Environment Unit
Group 4: Ruth – Roland Möllby, MTC
Note! Preparation necessary
12:00
Lunch
13:00
Lecture: Chemical hazards in laboratory work (I)
Ulrika Olsson, Security and Environment Unit
Note! Preparation necessary
14:15
Film: Laboratory accident
Ulrika Olsson, Security and Environment Unit
14:30
Coffee
14:50
Lecture: Allergens in the laboratory
Ann-Sophie Merritt, IMM
15:30
-16:15
Lecture: Fire safety
Christer Matonog, Security and Environment Unit
*Presence obligatory. Absence can not be compensated for during this course.
Day 2. Tuesday, November 10th
Time for self-studies
Web-tutorials and assignment are performed in Ping-Pong.
Please note, dead-line for web-tutorials and assignment below is 9 am on Wednesday
Web-tutorials:
Chemical hazards in laboratory work (II)
Responsible teacher: Ulrika Olsson
Introduction to the chemical database KLARA
Responsible teacher: Ulrika Olsson
Cancer risks
Responsible teacher: Ingela Djupedal
Cytostatic drugs - introduction
Responsible teacher: Ulrika Olsson
Ventilated workplaces
Responsible teacher: Ingela Djupedal
Personal protection: Gloves
Responsible teacher: Ingela Djupedal
To be discussed in Group seminar on Wednesday morning
Self test:
Transport of dangerous goods
Responsible teacher: Ingela Djupedal
Assignments:
Evaluation and improvement of a risk assessment
Responsible teacher: Ulrika Olsson
To be discussed on Group discussion II on Wednesday at 13.00
Day 3. Wednesday, November 11th
Rooms for practical sessions :
Rooms for group practice:
9:00
MTC course lab, Scheeles väg 2
Rolf Bergin and Gunnar Höglund, Berzelius väg
3, 4th floor.
Group seminars *
i) Gloves, Ingela Djupedal
ii) Handling and storage of chemicals, Ulrika Olsson
iii) Contamination risks in the laboratory, Helene Fredlund & Mats Spångberg
iv) Incidents- spill, Jenny Karlsson
Session rotations
9:00-9:35
Group 1
Group 2
Group 3
Group 4
i) Gloves
ii) Handling &
storage of
chemicals
Gunnar Höglund
iii) Cont. risks in
laboratory
iv) IncidentsSpills
Course lab
Course lab
ii) Handling &
storage of
chemicals
Gunnar Höglund
i) Gloves
iv) IncidentsSpills
iii) Cont. risks in
laboratory
Rolf Bergin
Course lab
Course lab
iii) Cont. risks in
laboratory
iv) IncidentsSpills
i) Gloves
Course lab
Course lab
Rolf Bergin
ii) Handling &
storage of
chemicals
Gunnar Höglund
iv) IncidentsSpills
iii) Cont. risks in
laboratory
Course lab
Course lab
ii) Handling &
storage of
chemicals
Gunnar Höglund
Rolf Bergin
9:45-10:20
10:40-11:15
11:25-12:00
12:00
i) Gloves
Rolf Bergin
Lunch
Lecture hall: Wretlind, Widerströmska Huset , Tomtebodavägen 18A,
Group rooms: Group room 1, 2, 3 and Ruth, Widerströmska Huset ,Tomtebodavägen 18A
13:00
Group discussion II:
Assignment Evaluation and Improvement of a risk assessment *
Group 1: Group room 1 – Ulrika Olsson, Security and Environment Unit
Group 2: Group room 2 – Jenny Karlsson, Security and Environment Unit
Group 3: Group room 3 – Ulrika Olsson, Security and Environment Unit
Group 4: Ruth – Jenny Karlsson, Security and Environment Unit
Note! Preparation necessary, see assignment day 2, Tuesday
14:00
Coffee
14:20
Lecture: Conclusions and questions on chemical hazards (mentometer exercise)
Ulrika Olsson, Security and Environment Unit
15:10
Break
15:20
-16:10
Lecture: Biosafety I: Infection routes in the laboratory and laboratory acquired infections
Roland Möllby, MTC
* Presence obligatory. Absence can not be compensated for during this course.
Day 4. Thursday, November 12th
Computer room: Ada Lovelace and Group room BZ-408, Berzelius väg 3, 4th floor
09:00
Lecture: Laboratory waste management
Ingela Djupedal, Security and Environment Unit
09.30
Computer assignment: waste management
Assignment to be done by Friday!
The assignment is mandatory and should be performed in groups of two
or three. However, presence at this session is not mandatory, the assignment
may be performed elsewhere but submitted by Friday at the latest.
Please bring your own laptop to this session if you want to work in the Group
room instead of the Computer room.
10.30
Conclusions and discussion on waste management assignment
Ingela Djupedal, Security and Environment Unit
10:45
Time for self-studies
Web-tutorials are performed in Ping-Pong.
Please note, dead-line for web-tutorials below is 9 am on Friday
Web-tutorials:
Definitions of biological specimens
Responsible teacher: Jenny Karlsson
Risk assessment- biological agents
Responsible teacher: Jenny Karlsson
Biosafety II - Containment principles
Responsible teacher: Roland Möllby
Biosafety level 3 and 4 laboratories (BSL3 and BLS4)
Responsible teacher: Jenny Karlsson
Disinfection and sterilization
Responsible teacher: Roland Möllby
Blood and human samples
Responsible teacher: Roland Möllby
Genetically modified microorganisms (GMM)
Responsible teacher: Jenny Karlsson
Assignment:
Green laboratory practices
Responsible teacher: Ann Rämme Strömberg
To be discussed on Friday at 13.00
Day 5. Friday, November 13th
Lecture hall: Wretlind, Widerströmska Huset , Tomtebodavägen 18A
Group rooms in the morning: Parker, Tomtebodavägen 18A and MTC course lab,
Scheeles väg 2
Group rooms in the afternoon: Group rooms 1, 2 ,3 and Ruth, Tomtebodavägen 18A,
9:00
Lecture: Biosafety III: The Biosafety Level 2 (BSL2) Laboratory
Roland Möllby, MTC
9.30
Biosecurity and dual use
Roland Möllby, MTC
9:50
Coffee
10:10
Lecture: Conclusions and questions on biosafety (mentometer exercise)
Roland Möllby, MTC
Jenny Karlsson, Security and Environment Unit
11:00
Break
11:15
Focus groups in laboratory safety
Select one focus group that interests you, you may choose between:
i) Blood handling
Roland Möllby, MTC
Room: Wretlind
This focus group is for people working with blood in the laboratory. We will discuss
problems at the working site such as instructions, waste management, transport, etc.
ii) Needle handling exercise Helene Fredlund & Mats Spångberg, AFL, KM
Course lab
The exercise will focus on practical handling of needles from a biosafety point of view.
Techniques to avoid skin puncture accidents, aerosol formation and splash will be
practiced. In vivo and in vitro examples are demonstrated and practiced. (Max 12 students)
iii) GMM-retro- and lentiviral vectors Alf Grandien, MedH, KI Group room: Parker
Retro- and lentiviral expression vectors have become more and more appreciated tools in
many scientific fields. There are some aspects concerning biosafety of these vectors that
one should be aware of. My plan is to discuss these issues or any other issue concerning
use of these or similar vectors that you would like to bring up.
12:00
Lunch
13:00
Lecture: Sustainability aspects on laboratory work
Ann Rämme Strömberg, Security and Environment Unit
13:45
Coffee
14:00
Lecture: Radiation safety
Mats Stenström, Security and Environment Unit
14.4515.30
Group discussion III: Follow up group discussions Own experiences *
Group 1: Group room 1 – Carina Bengtsson, MTC
Group 2: Group room 2 – Ingela Djupedal, Security and Environment Unit
Group 3: Group room 3 – Jenny Karlsson, Security and Environment Unit
Group 4: Ruth – Roland Möllby, MTC
Note! Preparation necessary, see Group discussion 1 on Monday, November 9
* Presence obligatory. Absence can not be compensated for during this course.
Day 6. Monday November 16th
Lecture hall: Hillarpsalen, Retzius väg 8
Computer rooms: Ada Lovelace and Grace Hopper, Berzelius väg 3, 4th floor
10:00
-12:00
Examination: Course content. Approximately 2hours * Note Hillarpsalen
Roland Möllby, MTC
Ingela Djupedal, Security and Environment Unit
11:00
Coffee
13:00
-15:00
Examination: Risk assessment
Ada Lovelace and Grace Hopper
Jenny Karlsson, Security and Environment Unit
Ulrika Olsson, Security and Environment Unit
Presence at this session is not mandatory but it is mandatory to perform a risk
assessment. Performed in groups of 2-3, see further instructions in Ping-Pong.
Deadline for handling in risk assessment is November 23, 2015
* Presence obligatory. Absence can not be compensated for during this course.
There will be an opportunity to re-do the exam on November 27, 13.00-14.00 in lecture hall
Wangari.
Widerströmska Huset
Tomtebodavägen 18A,
Lecture halls
Wretlind and Wangari
Group rooms
Group rooms 1, 2, 3 ,
Ruth and Parker
Retzius väg 8
Hillarpsalen
Berzelius väg 3, 4th floor
Computer rooms
Scheeles väg 2
Course lab