MONOCYTE ENRICHMENT Elutra® cell SEPARATION SYSTEM A Functionally Closed System for Monocyte Enrichment without Pre-Processing or Expensive Antibodies The Elutra system uses counter-flow centrifugal elutriation―fluid flowing through cell layers in a centrifugal field―to enrich monocytes based on size and density. Predictable monocyte enrichment direct from leukapheresis products in less than one hour… from set up to completion Elutra system’s reproducible process is designed to provide 60 percent monocyte recovery* n 80 percent monocyte purity* n Enrichment with the Elutra System is fast and easy Connect your starting cell product to the Elutra system’s disposable tubing set n Set up and prime the tubing set n Load the separation chamber with cells n Select and perform the desired separation profile (automated red blood cell debulking optional) n Disconnect the enriched monocyte product bag from the tubing set n Monocyte enrichment in the Elutra Separation Chamber Unlocking the Potential of Blood * Typical results based on cell counts from a leukapheresis product collected the previous day from normal donors on a COBE® Spectra™ Apheresis System. 80 to 230 ml products contained 5 to 14 x 109 WBC count, 80 to 100% mononuclear cell, and 1.8 to 4.7 ml red blood cell volume. MONOCYTE ENRICHMENT Elutra cell SEPARATION SYSTEM High Purity, High Yield Monocytes Initial TNC N=7 5.9 -10.3 x 10 % of Monocyte Recovery % of Monocyte Purity 67 ± 15 75 ± 13 9 Read E.J., et al, NIH Department of Transfusion Medicine, Bethesda, MD; Gambro BCT, Lakewood, CO. 2003. Evaluation of a Closed Automated System to Isolate Peripheral Blood Monocytes for Dendritic Cell (DC) Immunotherapy. Ninth annual meeting of the ISCT, 29 May–1 June. Elutra Combines Convenience and Performance Automated Optional Red Blood Cell Debulking of Leukapheresis Products Containing more than 4 mL of Red Cells D esigned to Provide High Purity and Recovery of Monocytes without Gradient Solutions A llows consistent processing of Cellular Starting Material in a Single Run* Semi-automated system with nine User-Defined Separation Profiles Helps Standardize the Enrichment Process User-Friendly Interface that Guides the Operator Through System Set Up and Prompts when to Initiate the Next Fraction Collection Reduces the Risk of Operator Error Unique Conical Separation Chamber Designed to Process from 5 x 109 to 20 x 109 Nucleated Cells* Red blood cell debulking in the elutra separation chamber * Typical results based on cell counts from a leukapheresis product collected the previous day from normal donors on a COBE Spectra system. 80 mL to 230 mL products contained 5 to14 x 109 white blood cell count, 80 to100% mononuclear cell, and 1.8 mL to 4.7 mL red blood cell volume. Note to U.S. and Canadian Customers: Terumo BCT considers the Elutra system for laboratory use and not subject to pre-market clearance by the U.S. Food & Drug Administration or licensure by Health Canada. Cells processed on this system, which are intended for direct transfusion or are used in the production of a therapeutic product(s) or vaccine(s) for clinical use, may require advance regulatory clearance or approval, which is the sole responsibility of the user. Terumo BCT, Inc. Terumo BCT Europe N.V. Terumo BCT (Asia Pacific) Ltd. Terumo BCT Latin America S.A. Terumo BCT Japan, Inc. 10811 West Collins Ave. Lakewood, Colorado 80215-4440 USA Europe, Middle East and Africa Ikaroslaan 41 1930 Zaventem Belgium Room 3903-3903A, 39/F ACE Tower, Windsor House 311 Gloucester Road Causeway Bay, Hong Kong La Pampa 1517 - 12th Floor C1428D2E Buenos Aires Argentina Ebisu South One Bldg. 9F 1-7-8, Ebisu-minami, Shibuya-ku, Tokyo 150-0022 Japan Phone: +32.2.715.05.90 Fax: +32.2.721.07.70 Phone: +852.2283.0700 Fax: +852.2576.1311 Phone: + 54.11.5530.5200 Fax: + 54.11.5530.5201 Phone: +81.3.6743.7890 Fax: +81.3.6743.9800 USA Phone: 1. 877. 339.4228 Phone: +1. 303 . 231.4357 Fax: +1. 303 . 542 .5215 UNLOCKING THE POTENTIAL OF BLOOD | TERUMOBCT.COM ©2008 Terumo BCT, Inc. /PN 306670497