Invitation ZEISS Technology Workshop We cordially invite you to our ZEISS Technology Workshop in Madrid. Data: 16th - 19th February Centro Nacional de Investigaciones Cardiovasculares CNIC C/ Melchor Fernández Almagro, 3 28029 Madrid Registration can be done through between the 15th October and the 20th January 2015 Inscription before the 10th January 2015: 250€ + IVA Inscription between the 11th and the 20th January 2015: 350€ + IVA Confirmation of assistance will be announced and payments will be done by transfer after closing the registration on the 20th January 2015. The minumum number of participants is of 10 and a maximum of 25. ZEISS Technology Workshop Agenda Monday, 16th February 2015 11:30 Registration and welcome (CNIC Ground Floor Desk) 12:00 Presentatation ZEISS Technology Workshop 12:15 Fundaments of microscopy. Preconditions for imaging: Contrast, resolution and magnification. How to create an image: beam path, contrasting methods (BF, DF, DIC, Pol, FL). 13:30 Objectives, light sources and how to choose correctly. Types of objectives and corrections, graving on objectives and eyepieces. Light sources: electrons, XRays, LightSheet, Lasers, HAL, Colibri, HBO, HXP, LED... 14:30 Lunch (CNIC Canteen) 15:30 Principle of detections. Cameras: sensors, definitions (dynamic range, full well capacity, resolution, interpolation, colour co-site sampling, micro scanning…). Adaptors. PMT/GaAsP/ electron detection... Noise, types, signal to noise ratio. 16:30 Types of microscopes. Stereomicroscopes, Axio Zoom, light microscopy (upright, inverted), fluorescence micros, optical sectioning, confocal, superres., LightSheets, electron microscopy, Xray... Scale of size. Presentation of Zeiss portfolio and new technologies. Agenda Tuesday, 17th February 2015 09:30 Tomography: Principles with SEM and XRM 10:30 Principles of Fluorescence Microscopy. Excitation/emission spectra, Stokes shift; fluorescence lifetime and molecular brightness. 11:30 Fluorescent dyes. Chemical tags, genetic tags; solvent and temperature effects; photobleaching; labelling approaches. Sample preparation in vitro vs in vivo 12:00 Lunch (CNIC Canteen) 13:00 Optical Sectioning I. Linear microscopy definition: Eliminate FL from out of focus planes. Advantages, disadvantages. Systems for optical sectioning and applications: Apotome, confocal, SD, LightSheet. How to get the best of your confocal 14:30 Coffee Break (CNIC Canteen) 14:45 Hands on Wednesday, 18th February 2015 09:30 Solving confocal limitations: LSM 880, AiryScan + Supperres 10:45 Optical Sectioning II. Non linear microscopy: TIRFM, Multiphoton and second harmonic. Principles, lasers, application. Microscope set up: how to get the best of your MP 12:00 Lunch (CNIC Canteen) 13:00 Image analysis. Bit depth, grey scale, LUT, colocalisation, image resolution vs optical, image size vs pixel size… 14:00 Biostatistics and presentation of results (charts, pie, point graph…) 15:00 Coffee Break (CNIC Canteen) 15:15 Hands on Agenda Thursday, 19th February 2015 09:30 Fluorescence Lifetime Imaging (FLIM) 10:15 Multi-modal Föster Resonance Energy Transfer (FRET) 11:15 Coffee Break (CNIC Canteen) 11:30 Motility, diffusion dynamics by photo-perturbations: FRAP, FLIP and FLAP 11:45 Motility, diffusion dynamics by fluorescence fluctuations: FCS, RICS and N&B 12:30 Hands on