From www.bloodjournal.org by guest on December 22, 2014. For personal use only.
A HEMATOPOIETIC
PERIFOLLICULAR
ENVELOPE
IN
THE
RAT
SPLEEN
E.
By
I
T IS well
the
these
known
spleen
that
of rats
and
other
myelocytes,
usually
as
general
and
occurring
notice,
however,
envelope
of hematopoietic
cells
is separated
from
the follicular
responds
to hematopoietic
pathologic
which
it
it surrounds.
To
the follicle
is sharply
a Follikdaussenzone.
Also,
Dunn
packed
pale
writes
cells.
that
he
Dr.
‘ ‘
R. D. Lillie,
of
ogous to the ellipsoids
[R.D.L.]
it has
seemed
ofdogs
sinuses.
low,
It is often
we
to
have
conditions
pulp.
and
during
vary
the
in general
area
our
size
conditions,
The
modified
tissue,
depleted
when
exposed
of active
blood
cell
preparations
ylene
blue
tried,
leukocyte
spleen
studied
stained
and
were
with
azur
poietic
From
of considerable
poisons,
it
system
of the
soon
School
of Medicine,
in
University
of closely
was
performed,
mesh
variable.
content
of
to
homol-
To me
the
As will
amount
paraffin
eosin.
Other
Details
and
the
in evaluating
became
areas
used.
of several
pathologic
collars
is quite
pulp
and
appear
be-
under
different
of blood
be rather
to hematopoietic
routine
counts,
the
rind.
Svirbely,
content
it seems
Strasser2
in
an
the
red
auxiliary
poisons,
and
exu-
regeneration.
and
also
help
hematopoietic
the
were
differential
by
and
a thin
animals,
work
and
certain
STUDY
mostly
hematoxylin
i-eosin
and
of the hematopoietic
the course
of a study
distinct
were
in size
at least,
periods
blood
an
the follicle,
from which
reducing
accumulation.”
greatly
PRESENT
thickness)
the
of hemosiderin
its
this
pressure
esis
Under
than
other
are
lacks
sinuses,
tissue,
and
pulp.
perifollicular
the
with
to vary
to have
under
and
Its conspicuousness
inversely
site
red
cells
follicle
or Aussenone
laboratory
as possibly
cats.
the
pulp,
cells,
apparently
called
a Randone
of sinuses
and
the
of
in whose
zone
and
principal
found
However,
hematopoietic
berant
the
to
this
has
voluminous
in rats
follicles
in the
plasma
These
It
of a Follikeihof
narrowing
Malpighian
Malpighian
does
more
which
Weidenreich4
also noted
absence
‘ ‘
in
to
well circumscribed,
or rind of connective
than
of benzene
normally
In addition
be found
eosinophils,
(Jaffe’).
the
be
to
Jaff#{233}
speaks
toxicity
noted
in the
also
clumps.
surrounding
appear
be sure,
conceives
‘ ‘
small
differently
may
separated,
Strasser
in a study
of the
and Von Oettingen3
in
occur
animals.
erythrocytes
or
which
is fairly
cells by a shell
stimuli
conditions,
found
cells may
neutrophils,
nucleated
that
kinds
as of other
cells
diffusely
PH.D.
of various
as well
arterioles,
these
and occasionally
megakaryocytes,
caped
cells
rodents
reticulo-endothelial
and accompanying
small
together
with erythrocytes
described
M.D.,
KRUMBHAAR,
hematopoietic
and
lymphocytes
B.
the
hundred
effects
apparent
to
the
of Pennsylvania,
953
sections
such
records
and
findings.
rodents,
of mustard
that
addition
of the
weight
cut
stains,
the
During
chiefly
gas
spleen
Philadelphia.
of the
gross
lymphocyte-forming
(6-8
micra
in
as eosin-methexperiments
appearance
the
of the
examination
adult
rats, during
and other
hemato-
contained
two
rather
Malpighian
From www.bloodjournal.org by guest on December 22, 2014. For personal use only.
E.
954
follicles.
These
follicular
areas
were:
either
myelocytic,
mixtures.
In the
which,
as Jaffe
small
foci
referred
to.
(i)
already
B.
KRUMBHAAR
scattered
The
erythrocytic,
monocytic,
case of the lymphocytes,
notes,
can
be brought
out
with
appearance
these foci
pleted
and
FIG.
rounded
the
field.
Normal
i.
rat’s
by the
paler
(H-E;
io
regeneration.
and
spleen.
staining
Two
the
follicles
envelope
red
pulp;
apparently
the
(2.)
of
cells, or sometimes
of
been about
small vessels,
perfused
specimens;
the
in recovery
(MF)
periods
cells
with
their
central
more
open
red pulp
(PFE).
The
case
with
different
knowledge,
they
during
arteries
of
active
appear,
occupies
However,
of normal
as so often
is the
present
state
responses.
of our
rats’
the perifollicular
spleens,
areas
units
at times
are easily
of the
sur.
the
rest
of
in width
from about
30
by a delicate
but definite
to as much
as ioo
line of collagenous
hemoly-
showed
unex-
distinguished
in the stained
specimen
as collars
of nucleated
cells, paler and larger
than
lymphocytes
of the Malpighian
follicle.
The “collar,”
a term appropriate
two dimensional
section
but not for what
is obviously
a three
dimensional
ture, varies
the follicle
pen-
composed
as those
in the envelopes
about
to be
tended
to disappear
in the spleens
de-
widespread
Maipighian
perifollicular
the
were
X)
topoietic
system
in the
pected
and unexplained
In sections
to be large
foci
or lymphocytic
they may have
some of these foci have the same
described.
As might
be expected,
by poison,
through
small
the
adult
for the
struc-
micra.
It is separated
from
connective
tissue which
is
From www.bloodjournal.org by guest on December 22, 2014. For personal use only.
HEMATOPOIETIC
presumably
tains
a development
a young
type
young
a deep
FIG.
the
follicular
and
sparse
in normal
spleens
Same
section,
near
the
photograph).
The
more
gradation
to
955
framework.
collagen
are
lymphoblasts,
the nuclei round
staining
central
dot, and with
2..
ENVELOPE
This
fibers.
narrow
The
rind
complete
circumference.
In one instance
(rat
band for about
one quarter
of the perimeter.
areas
lymphocytes
no
from
of fibroblast
be traced
around
the
was enclosed
in this
perifollicular
PERIFOLLICULAR
showing
part
margin,
of the
separated
homogeneous
the
nature
follicle
delicate
and
of the
collar
of the
thin
The
cells
about
the
of chromatin,
cytoplasm.
its collar.
connective
Note
tissue
than
of the
red
rind,
nor
do
the
curved
(which
does
pulp
is obvious.
they
concannot
a capillary
1630),
mononucleated,
and moderately
full
scanty
pale-staining
larger
by
fibroblasts
mostly
rind
usually
of the
size
of
often
They
with
show
rows
of adult
not
show
(H-E;
resemble
in
the
32.0
X)
them
in
appearance.
They
do not
granular
show
These
surface
and
no
appear
never
characteristic
cells
were
of some
Gr#{252}nwald-Giemsa)
Successive
to be monocytes,
contains
imprints,
30
ingested
as they
material.
have
scanty
The
nuclei
cytoplasm
are
never
that
is not
indented
and
nucleoli.
further
investigated
by
normal
rats’
and
with
a dozen
stained
or
spleens
sections
more
per
making
imprints
comparing
from
spleen,
the
of the
the
other
reproduced
freshly
stained
side
the
bisected
imprint
of
the
same
(Maybisection.
appearance
From www.bloodjournal.org by guest on December 22, 2014. For personal use only.
956
E.
with
surprising
sections.
Though
good
blood
to be
young
the
smears,
their
where
Note
and
Oxidase
cytoplasm
such
The
possibility
be ruled
and
and
out
such
larger
collar,
into
as
no
red
cells
can
their
heavily
and
these
mode
of
eosinophils;
sinuses.
pulp,
The
the
under
135
could
thought
Compared
normoblasts,
in
chromatin
clumps
The
cytoplasm
was
pale
blue
without
after
nitrogen
imand
mostly
granules
mustard
immature
to
the
coarse
of polvs,
or
gas poisoning.
red
cells
and
debris
in
their
X)
not
be
monocytes
living
growth
demonstrated
and
or myeloid
conditions,
observed.
cells)
and
erythrocytes,
large,
No
margin
being
adult
pale
are
found,
vesiculated
and
have
of the
zone
chiefly
determined
merges
cannot
cultures,
Occasional
more
sparsely
polymorphonuclear
nuclei
megakaryocytes
cells
as in tissue
locomotion
(immature
also
outer
(H-E;
stain)
cells.
transition
days
a number
are immature
nuclei
normoblasts
seventeen
were
myself.
serial
do in
neutrophils.
be made
stained
reticulo-endothelial
and
the
that
a very
collar.
(benzidine
in the
studies
constantly,
neutrophils
he adult
granules
dying
cells, with
and
and
with
They
as by
membrane.
it stained
follicle
identified.
as well
paler,
nuclear
many
separating
easily
lymphocytes
larger,
rat 6-1-7,
lost
were
adult
the
of
have
as appeared
items
of
abundant,
spleen
collar
until
round,
not
more
if superficial,
resemblance
to
as well by this method
as they
colleagues
per cent
o
the fibrocvtes
vacuoles.
several
around
depleted
follicle
present.
were
condense
3. Greatly
the
in question
by
nuclei
nuclei
to
scanty;
FIG.
Both
cells
dark-staining
a tendency
very
the
KRUMBHAAR
given
a marked,
cells did not stain
lymphocytes
small
prints
similarity,
the blood
B.
ever
that
are
been
found
taken
almost
imperceptibly
by
appearance
the
to
in the
of
From www.bloodjournal.org by guest on December 22, 2014. For personal use only.
HEMATOPOIETIC
sinuses
and
that
cut
follicular
the
none
of the
for
rind
next
figure
wide
with
In spleens
collars
occasional
the
megakaryocyte,
red pulp
collars
In rare
mitoses
local
killed
three
weeks
More
interspersed
the
however,
As
the
the
more
dark
from
of some
larger
and
small.
collars
and
conspicuous
blue
hematocytic
of the
dose
The
months
cells
sometimes
the
follicle
regeneration
a still
damaged
three
weeks
indicates
(H-E;
the
normal
few
the
experiments,
with
pale,
of the
pulp
of
the
larger
kinds,
cells
except
foci
erythrocytes,
in the
field
X)
io
of several
red
earlier.
the
in concentrated
relatively
and
by
If the
given
rectangle
after
than
only
circular
margins
pulp.
indicated.
paler
contain
two
granulocytic
empty
contrast.
a non-lethal
hematopoietic
collars
its
in bold
to three
appear
on
out
at all.
and
red
than
follicle
an almost
of the
is relatively
width
found
is congested,
instances,
that
present.
are
between
recovering
true
by
stroma
Sections
perifollicular
lymphocytic
bounded
stands
of the
always
production.
appear
while
of erythrocytes.
of the
of the
denser
957
a broader
be represented
stroma)
of a follicle
killed
not
poisons
the
number
show
lymphocytes
collar
an approximation
but
and
and
empty
collar
of rats
usually
suggest
the
greater
may
scanty
ENVELOPE
may
adult
collar
of rat 6-1-3,
extremely
much
a follicle
by various
an extremely
follicular
4. Spleen
the
the
or even
is congested,
FIG.
the
of
perifollicular
recognizable
Note
by
edge
depleted
the
(except
spleen
and
the
spleens
series,
band
stroma
area,
In the
cell
more
through
PERIFOLLICULAR
the
reddish
that
more
purple,
erythrocytes.
foci
may
he so
From www.bloodjournal.org by guest on December 22, 2014. For personal use only.
958
E.
extensive
with
that
their
more
usual
erythrocytes
cells
than
B.
KRUMBHAAR
are
in
the
found
pulp,
diffused
which
through
the
is hardly
collars
together
recognizable
as splenic
tissue.
Turning
to
nucleated
other
cells
follicle
are
more
occasional
The
suggestions
Same
.
in the
section
(8oo
(mega),
In the
rabbit,
the
picture
of the
dency
for
collar
varies
to
ful,
though
has
the
inside
the
rat.
The
with
red
developed,
transition
from
indistinguishable,
may
be seen,
in the pulp.
pulp
right)
that
be
rind
are
area
surely
though
beyond
Here,
of the
found
is hard
the
in
rat,
in
which
too,
the
both
by
at
this
this
number
to distinguish,
red pulp.
except
the
the
numer-
though
that
tissue
pink
staining
place.
The
“pale
centers”
center
is usually
strip
cell
of
found,
and
cells
a tenof
that
the
the
by
population
of nonlymphocytic
the
(H-E)
topographic
of connective
separate
a fair
the
the
are
not
rind
and
rind.
which
scanty
and
ill-defined;
the
difference
normal
like
indicated
rat,
the
is less
and
differentiation.
of the
(to
often
to
rind
out
in the
may
tissue
pig,
Jaff#{233}says
tissue
is more
guinea
same
In a few
being
RBC
collar
pulp
is almost
farther
beginning
in the
sectioned
as in the
be found
are
rare
follicle
the
In the
collar
are
the
the
therefore
‘
aid
and
spleen,
throughout
than
The
(s),
cells?)
they
limits
‘collar’
pulp
X).
sinuses
(reticulo-endothelial
follicle;
‘
condensed
megakaryocytes
FIG.
mouse
frequent
of a connective
are more
megakarvocs’te
the
more
gradual.
lymphocytes
ous
rodents-in
much
hamsters,
may
collar
in cell
the
have
pale
centers
indeed,
even
is easy
to
population
perifollicular
their
find
from
in the
existence
if perhaps
the
tissue
follicle
is also
follicles,
both
in places
narrow.
as does
found
seems
When
that
to be less
doubtfound,
of the
constant
it
rat.
From www.bloodjournal.org by guest on December 22, 2014. For personal use only.
HEMATOPOIETIC
PER1FOLLICULAR
and rich than in rats. Few if any myeloid
normoblasts
and erythrocytes.
The rind
in small
cells
varies
are seen, ripe or unripe,
and very few
much
in size, not being found
at all
follicles.
SUMMARY
About
the
prominent
takes
959
ENVELOPE
Malpighian
follicles
in rats,
is a perifollicular
part
in hemolytopoietic
active
AND
of
CONCLUSIONS
several
species
envelope
of
composed
rodents,
and
especially
of hematopoietic
cells
that
changes.
The identity
of the mononuclear
cell comprising
the greater
part of this tissue
has not been positively
determined;
it is probably
a young
lymphocyte.
It is possibly the homologue
of the pale centers
of the Malpighian
follicles
in man and other
mammals,
though
It should
stains,
ture
and
and
of test
moving
pulp.
It often
clear
neutrophils,
in.
found
determine
poisons,
and,
in rabbit
this
spleens
identity
if possible,
which
by
also
have
pale
use of a greater
the
of dynamic
is separated
from the Malpighian
tissue,
but on its outer
margin
contains
a scattering
and
of these cells were
that colonization
dered
been
to
methods
such
centers.
variety
of
as tissue
cul-
pictures.
The envelope
enous
connective
any
has
it
be possible
The
never
follicle
merges
of erythrocytes,
eosinophils
and
so greatly
increased
under
was suggested;
they were
collar
noteworthy
rarely
it
contains
by a thin
gradually
normoblasts,
pigment-bearing
polymorphonumacrophages.
pathologic
later thought
Some
conditions
first
to have probably
tl-e
megakaryocytes
rind of collagwith
the red
or sinuses
or blood
studied
wan-
vessels
size.
REFERENCES
R.:
‘JAFFE,
1931.
2 STRASSER,
SVIRBELY,J.
Handbuch
Pp.
der Anatomic
und
Pathologic
der
KleinemLaboratoriumstiere.
Berlin,J.
Springer,
2.40-2.44.
ULRICH:
L.,
Beitr. a. Path.
DUNN,
R. C.,
Anat.
AND VON
70:
8,
OETTINGEN,
192.2..
W. F.:J.
Indust.
Hyg.
& Toxicol.
26: 37,
1944.
of
From www.bloodjournal.org by guest on December 22, 2014. For personal use only.
1948 3: 953-959
A HEMATOPOIETIC PERIFOLLICULAR ENVELOPE IN THE RAT SPLEEN
E. B. KRUMBHAAR
Updated information and services can be found at:
http://www.bloodjournal.org/content/3/8/953.full.html
Articles on similar topics can be found in the following Blood collections
Information about reproducing this article in parts or in its entirety may be found online at:
http://www.bloodjournal.org/site/misc/rights.xhtml#repub_requests
Information about ordering reprints may be found online at:
http://www.bloodjournal.org/site/misc/rights.xhtml#reprints
Information about subscriptions and ASH membership may be found online at:
http://www.bloodjournal.org/site/subscriptions/index.xhtml
Blood (print ISSN 0006-4971, online ISSN 1528-0020), is published weekly by the American Society of
Hematology, 2021 L St, NW, Suite 900, Washington DC 20036.
Copyright 2011 by The American Society of Hematology; all rights reserved.