From www.bloodjournal.org by guest on January 26, 2015. For personal use only. The Accumulation of p53 Abnormalities Is Associated With Progression of Mucosa-Associated Lymphoid Tissue Lymphoma By Mingqing Du, Huaizheng Peng, Nalini Singh, Peter G. Isaacson, and Langxing Pan The genetic mechanisms underlying the genesis of lowgrade mucosa-associated lymphoid tissue (MALT) lymphomas and their transformation into high-grade lymphoma are poorly understood. p53 inactivation, commonly caused by mutation and allele loss, has been shown t o play an imdisease portant role in the early development andlor the late progression of many humantumors including lymphoid main MALT lignancies and, thus, may also beimportant lymphomagenesis. We examined 75 cases(48low grade and 27 high grade) of MALT lymphoma for p53 allele loss and mutation as well as protein accumulation. DNA samples prepared from microdissected cell populations were used for the detection of p53 gene abnormalities. Loss of heterozygosity (LOH) of the gene was detected by polymerase chain reaction-based analysis of p53 CA repeat polymorphism, whereas p53 mutation was studied by single-strand conformation polymorphism analysis and direct sequencing. p53 expression was assessed by immunostaining with CM1 polyclonal antibody. p53 allele loss and mutation, which resulted in the alteration in the amino acid sequence, were found in both low-grade (LOH, 3 of 44 [6.89/01; mutation, 9 of 48 [18.8%1) and high-grade (LOH, 6 of 21 [28.6%3; mutation, 9 of 27 [33.3%1) MALT lymphomas, particularly in the latter group. p53 staining was not observed in any low-grade tumors but in 6 high-grade cases that harbored missense mutations. There were also differences in the extent of p53 abnormalities, between low- and high-grade tumors. Of the 11 low-grade tumors showing p53 abnormalities, only 1 tumor showed the concomitance of p53 mutation and allele loss, whereas in high-grade tumors, 6 of 9 affected cases displayed both p53 mutation and allele loss. Our results suggest that p53 partial inactivation may playan important role in the development of low-grade MALT lymphomas, whereas complete inactivation may be associated with highgrade transformation. 0 1995 by The American Societyof Hematology. M Various frequencies of p53 mutation have been documentedin lymphoid malignan~ies.‘~-~~ p53 mutation is thought to be associated with high-grade transformation of follicular lymphoma” and chronic lymphocytic leukemia.” Recently, a high frequency of p53 mutation has been reported in marginal zone splenic B-cell lymphoma,’8 which is analogous to MALT lymphoma in its origin of cell lineage, immunophenotyping, histology, and genetic findings such as the absence of bcl-2 and bcl-l rear~-angement.’~.’~ Thus, inactivation of the p53 gene may also be important in the tumorigenesis of MALT lymphoma. Most previous p53 studies have failed to pay full attention to the analysis of both allele loss and mutation together in the same tumor g r o ~ p , ” ~and ’ ~ thus the full potential of p53 inactivation in tumorigenesis may have been underestimated. In the present study, we examined p53 gene abnormalities, including both allele loss and mutation as well as protein accumulation in 75 cases of well-characterized low- and high-grade MALT lymphoma to determine the full potential of p53 inactivation in MALT lymphomagenesis. Our results suggest that p53 partial inactivation may be associated with the development of low-grade tumor, whereas complete inactivation may be important for high-grade transformation. ALIGNANT LYMPHOMA derived from mucosa-associated lymphoid tissue (MALT) accounts for the majority of extranodal lymphomas and arises from acquired MALT in several extranodal sites such as the stomach, salivary gland, and t h y r ~ i d . ”Low-grade ~ B-cell lymphoma usually exhibits an indolent clinical but is capable of transforming into high-grade tumor! Although the clinical, pathologic, and immunophenotypic features of MALT lymphoma have been well documented,”3 the genetic basis underlying its development and high-grade transformation is poorly understood. p53, a transcription factor, acts as a cell cycle check point proteinandinducescellcyclearrestinthelate G1 phaseor apoptosis after DNA di~nage.5.~ p53 inactivation, abolishing its tumor-suppressor activity, is the most common event in human m a l i p a n c i e ~ and ~ , ~contributestobothearlytumordevelopmentl0.” and late disease pro~ssion.’’The inactivation of p53 tumor-suppressor activity can be causedatthegenelevelby mutation,I3 deletion,I4 andarrangement'^ or at the protein level bybinding tooncogenicproteinssuch as MDM2.I6Among these, p53 mutation and allelic loss are the most common. Inactivation of p53 tumor-suppressor activity during tumor development is a process of accumulation of its genetic abnormalitie~.~,’~ p53 mutation can precede allele loss or vice versa.” In both circumstances, p53 function isonly partially inactivated because only one allele of the p53 gene is affected. Mutant p53 loses tumor-suppressor activity and may also exert a dominant negative effect on the remaining wild-type; however, it is unable completely to suppress wildtype function.19-*’p53 inactivation involving both alleles, usually by mutation in one allele and loss of the other, results in the complete loss of p53 function. These different p53 abnormalities have been shown to exhibit distinct tumorigenic potentials in p53 transgenic mice with increasing tumorigenicity after one allele loss, one allele mutation, and inactivation of both alleles.” These different p53 gene abnormalities may also be expected to be involved at different stages of human tumor development. Blood, Vol86, No 12 (December 15), 1995: pp 4587-4593 From the Department of Histopathology, University CollegeLondon Medical School, London, UK. Submitted June 7, 1995; accepted August 7, 1995. Address reprint requests to Peter G. Isaacson, DM, FRC Path, Department of Histopathology, University College London Medical School, Rockefeller Building, University Street, London WCIE SJJ, UK. The publication costsof this article were defrayed in part by page chargepayment. This article must therefore behereby marked “advertisement” in accordance with 18 U.S.C. section 1734 solely to indicate this fact. 0 1995 by The American Society of Hematology. 0006-4971/95/86/2-0035$3.00/0 4587 From www.bloodjournal.org by guest on January 26, 2015. For personal use only. DU ET AL 4588 MATERIALSANDMETHODS Tissuesamples. Seventy-five cases (paraffin-embedded tissues available in 63,frozen tissues available in 24) of B-cellMALT lymphoma were retrieved from the Department of Histopathology, UniversityCollegeLondonMedicalSchool. These comprised 48 low-grade and 27 high-grade MALT lymphomas. Tumors originated from the stomach in 60 cases, salivary gland in 6 cases, small intestine in 5 cases, and thyroid in 4 cases. The diagnosis of low-grade MALT lymphoma was established bythe characteristic histologic appearances’” together with immunophenotyping and clonal analysis oftheIgheavy chaingene. Of the27high-gradelymphomas, a low-grade MALT lymphoma component could be identified in 21, usually as one or more small foci present in paraffin sections from a minority of the blocks. Microdissection andDNA preparation. Paraffin (28 low-grade cases and 23 high-grade cases) and frozen (20 low-grade cases and 4 high-grade cases) sections were briefly stained with hematoxylin and eosin. Tumor andnontumorcellpopulationswereseparately microdissected from each case, as described previously.” The microdissected cell population was digested with 200 &mL proteinase K in a 40 to 1 0 0 pL solution containing 10 mmol/L Tris HCI (pH 8.3). 50 mmol/L KC1 at 37°C for 16 hours. Proteinase K was inactivated at 95°Cfor I O minutes. Celldebris was pelleted by centrifugation and the resulted supernatant was used for the subsequent genetic analysis of the p53 gene. Detection of loss of heterozygosit?,(LOH) of the p53 gene. LOH of the p53 gene was detected by polymerase chain reaction (PCR)basedanalysis of thep53 dinucleotide repeat (CA25) polymorphism.” The CA repeat was PCR-amplificd from both tumor and nontumor DNA preparations of the same patient using primers: GT strand, AGGGATACTATTCAGCCCGAGGTG, andAC strand, ACTGCCACTCCTTGCCCCATTC (OswellDNA Service, Department of Chemistry, Edinburgh University, Edinburgh, UK). One of theprimerswasend-labeledusing [y-”P] and T4 polynucleotide kinase. PCR products (2 yL) were mixed with 4 yL sequence stop solution (98% formamide, IO mmol/L NaOH, 20 mmol/L EDTA, 0.05%bromophenolblue,and0.05%xylene cyano1 FF), andthe mixtures were denatured at 98°Cfor 5 minutes and separated on 6% sequencing gel. The gel was routinely dried and exposed to an xray film for I to 2 days. Single-strand conformation polymorphism (SSCP) ana1.vsi.r. Because the majority of p53 mutations occur in exon 5, 6, 7, and 8 of thegene,’.’ these exons wereseparatelyamplified byPCR using primers described previously.”SSCP analysis of these PCR products was performed using our recently described protocol, which used polyacrylamide agarose composite gel together with a background free silver staining method and showed a high sensitivity (97%) for p53 mutation detection.” Briefly, PCR products (2 to 5 yL) were mixed with sequence stop solution. The mixtures were denatured at 98°C for 5 minutes, cooled on ice, and loaded onto a composite gel containing 5% acrylamide (acry1amide:bis-acrylamide = 99:l) and 0.25% agarose with or without 10% glycerol. Electrophoresis was performed at 6 W for 12 to 18 hours at 4°C maintained by a refrigerated circulator (Pharmacia, Uppsala, Sweden), and gel was subjected to silver staining. Direct DNA sequencing. Samples for whichmutationwas suggested by SSCP were further analyzed by direct DNA sequencing using fmol DNA sequencing system (Promega, Southampton, UK). Immunostaining of p53 protein. Immunostaining for p53 protein was performed in cases with paraffin-embedded tissues, which were composed of 37 low-grade and 26 high-grade tumours. For antigen retrieval, paraffin sections were treated in 0.01 moln sodium citrate buffer (pH 6.0) in a pressure cooker for 2 minutes before staining.25 G0015 N T G0078 N T Fig 1. Detection of LOH of the p53 gene in MALT lymphomas. p53 CA repeat was amplified by PCR, in which one of the primer was end-labeled (see the Materials and Methods). The PCR products were denatured and separated on 6% sequence gel. N and T indicate nontumor and tumor samples from the same patient. Case G0078 showed loss of one p53 allele in the tumor sample. p53 proteinwasroutinelyimmunostainedusingCM1polyclonal antibody (I/5,000 dilution) for 16 hours at room temperature, followedby Biotinylated Swine antirabbit Ig(11250 dilution) for 40 minutes and StreptABComplexRIRP (11100 dilution) for 40 minutes. Finally, sections were visualized by incubation with diaminobenzidine (DAB) and HzOz.All reagents used for the staining were purchasedfromDako(HighWycombe,UK).Foreachexperiment, positive (a breast carcinoma known to express mutant p53 protein) and negative controls (without the primary antibody) were included. Immunostaining for p53 using more concentrated CM1 antibody (either U2.500 dilution for 16 hours or 111,OOO dilution for 1 hour) wasadditionallyperformed in someoftheMALTlymphomas, which showed negative staining for p53 when CM1 antibody was usedat115.000 dilution. RESULTS LOH qf the p53 gene in MALT lymphoma. Of the 15 MALT lymphomas studied, nontumor DNA preparations were available from 74 cases. Among these cases, 65 (87.8%) were heterozygous for the p53 CA repeat and thus were informative for LOH analysis. Because the analysis was performed from DNA samples prepared from microdissected cell populations, this largely eliminated the interference from nontumor cells and LOH was easily determined by a direct comparison of the allelic density between tumor and nontumor samples of the same patient (Fig l). In total, LOHof the p53 gene was found in 9 (13.8%) cases, with a much higher frequency in high-grade (6/21 [28.6%]) than in lowgrade (3/44 [6.8%]) tumors. Mutation of the p53 gene in MALT lymphoma. Using PCR-SSCP analysis, mutations suggested by the altered electrophoretic mobility were identified in 21 cases and these From www.bloodjournal.org by guest on January 26, 2015. For personal use only. 4589 p53 INACTIVATION IN MALTLYMPHOMA 1 2 3 4 5 6 7 8 9 1 0 Fig 2. Detection of p53 mutation in MALT lymphomas byPCR-SSCP analysis. PCR products of p53 exon6 were denatured, separated o n polyacryl(see amide-agarose gel, and shown by silver staining the Materials and Methods). Lane 1 and 10, placenta 2 through 8, individDNA as negative controls; lanes ual MALT samples; lane 9, A3/kawa cell line containing a missense mutation in exon 6'' used as a positive control. Lanes 4 (case G00151 and 6 (case 60114) showed migrating fragments different from the normal control. mutations occurred in exon S in 7 cases, exon 6 in 8 cases. exon 7 in 4 cases, andexon 8 in 2 cases. Examples are shown in Fig 2. To confirm the results obtained by SSCP analysis and to characterize the nature of these mutations, SSCP-positive cases were further analyzed by direct sequencing. The results are presented in Table 1 and an example is shown in Fig 3. Mutation was confirmed in all cases. In 18 cases, mutations lead to alterations in amino acid sequence, most frequently amino acid substitution caused by missense mutations (point mutation in 14 cases; tandem double mutations in 1 case) and less frequently truncated protein due to frame shift mutation (l-bp deletion in 2 cases) and nonsense mutation ( I case). Mutations in the remaining three cases weresilent and did not alter the amino acid sequence. The mutations identified showed a wide spectrum with a similar frequency of transition (10/20) and transversion (10/20) mutations. Four of 20 mutations, including 2 C+T, l G"A transitions and I G"T transversion, occurred atCpG dinucleotides. However, no predominant type of mutation was seen. In summary, inactivating p53 mutation, which alters amino acid sequence, was found in l8 of 75 (24%) MALT lymphomas. Similar to LOH of the p53 gene, mutation is more frequent in high-grade (9/27 [33.3%]) than in lowgrade (9148 [ 18.8%]) tumors. Accunrulntiorl of p53 protein it1 MALT iwlpkotncr. Of Table 1. Summary of MALT Lymphoma Cases Showing p53 Abnormalities Case Malignant Grade Tumor Site G0015 G0318 G0078 G0178 G2098 G0123 G0062 G0177 G0208 G021 1 G1181 G2099 G0525 LG LG LG LG LG LG LG LG LG LG LG LG LG st st st st G1201 G1200 G0168 G0114 G0210 G 1207 G0016 G0399 G1918 G 1205 HG HG HG HG HG HG HG HG HG HG p53 Staining - LOH SSCP ND - 6+ Exon - + - ND + - - - st - Thy - Exon 6 + 5+ Exon -ATG 1605+ Exon 6+ Exon - 1655+ Exon 8+ Exon 5+ Exon 5+ Exon + 7+ Exon - - - - - - + - st st st st st st SG st st st ST st st st st - - - - + + st + + SI + Codon + + + + + + 7+ Exon Exon 6 + Exon 6+ 6+ Exon 158 5+ Exon 8+ Exon 5+ Exon Exon 6 + Exon 7 + 7+ Exon 197 GTG 196 178 CGA CAC 197 GTG CAG CGT CAG CAG ATG 273 165 165 243 253 206 194 212 274 151 192 248 254 Abbreviations: St, stomach; SG, salivary gland; Thy, thyroid; SI, small intestine; ND, not done. * Deletion. -- Mutation - CAT 1936+ Exon CAC GTA -------- ---------- Amino Acid Substitution His Val His Val TGA *AC CTG Val ATG CTG Arg TGT CCG CCG GTG Arg End Frameshift Met Leu Met Gln Leu Cys Gln Pro Gln Pro Met Val ACC ACT l T G T*G CTT -CAT "r-lTA CGC CTC GTT GAT CCC CAC CAG CGG CGG CAG ATC GAC Thr Thr Frameshift Leu His Phe Leu Arg Leu Val Asp Pro His Gln Arg Arg Gln Ile - A s p + + From www.bloodjournal.org by guest on January 26, 2015. For personal use only. DU ET AL 4590 GO016 ‘T Fig 3. Direct sequencing of the PCR product from SSCP-positive cases.CaseG0016 showed amissense mutation (CCC + CAC) in codon 151. the 63 cases in which p53 staining was performed, positive staining was found in 6 cases. All of the positive cases (6/ 26 [23%]) were high-grade tumors and none of the 37 lowgrade tumors studied showed any staining for p53. Nuclear staining was found in the majority of tumor cells in the positive cases. Strong nuclear staining was observed in 5 cases, whereas moderate nuclear staining wasseen in the remaining case. Of the positive cases, 1 case showed the presence of abundant low- and high-grade tumor components together with follicles colonized by high-grade tumor cells. p53 staining in this case was observed mainly in high-grade blasts, but also in a few low-grade tumor cells (Fig 4). Interrelationships between p53 mutation and p53 staining or p53allele loss in MALT lymphoma. Cases showing p53 staining, LOH, and mutation are summarized in Table 1. All tumors showing p53 staining contained missense mutations in the p53 gene. However, 14 cases harboring p53 mutations, including 9 cases with missense mutations, 2 cases with silent mutations, 2 cases with frameshift mutations, and 1 case with a nonsense mutation, did not show any staining for p53 in at least two separate staining experiments using more concentrated CM1 antibody. In addition to the difference in the frequency of p53 allele loss and mutation between low- and high-grade tumors, difference in the extent of p53 abnormalities was also observed between the two groups. Of the 11 low-grade cases presenting p53 abnormalities, only 1 case showed the concomitance of p53 mutation and LOH, whereas in the high-grade tumours, 6 of 9 involved cases displayed both p53 mutation and LOH, suggesting the association between the accumulation of p53 abnormalities and disease progression. high-grade lymphoma has resulted from transformation of low-gradedisease. This assumption is reinforced by the finding in such cases of the same Ig light chain restriction in both lesions4 and a recent case report36 of identical Ig gene rearrangement. In this context, it has been shown that the chances of identifying a low-grade component are increased as more sections are examined: Even when no lowgrade component has been identified, as in 7 of our cases, a relationship to MALTlymphoma cannot be excluded. Factors that support this relationship include the association with preceding Helicobacter pylori infection inbothlow-and high-grade gastric lymph0mas,3~”*the frequent presence of chromosome trisomy 3,39 and the similar survival statistics reported in high-grade cases with or without a low-grade component.40 To understand the full potential of p53 inactivation in MALT lymphomagenesis, we have examined gene abnormalities, including both mutation andallele loss, and protein expression in a large series of low- and high-grade MALT lymphomas. Several precautions were taken in the present R DISCUSSION The relation between low-grade MALT lymphoma and high-grade B-cell lymphoma arising in mucosalsites is problematic. Where foci of low-grade MALT lymphoma rm he detected in the same specimen: as was the case in 21 of 27 cases in our study, it seems reasonable to assume that the Fig 4. Accumulation of the p53 protein in MALT lymphomas. p53 was immunostained with CM1 antibody. Case GOll4showed nuclear staining in majority of the high-grade (HGI but also in a few of the low-grade (LG) tumor cells. The high- and low-grade foci are from the same histologic section. From www.bloodjournal.org by guest on January 26, 2015. For personal use only. p53 INACTIVATION IN MALT LYMPHOMA study to ensure the maximal detection of p53 abnormalities. First, microdissection was performed in each case to isolate both tumor and nontumor cell populations, from which p53 gene analysis was performed. This has been proven to be the crucial step because MALT lymphoma frequently coexists with reactive components and many of our samples contained only a small tumor mass. The DNA isolated from a whole tumor section or tissue may be adequate for SSCP analysis in some cases but is far below the requirement for direct sequencing and LOH analysis due to the presence of abundant nontumor cells (our unpublished results). Secondly, a highly sensitive SSCP analysis using polyacrylamide agarose composite gel and a background free silver staining method was used in the present study, which, in a previous was shown to detect 97% of p53 mutations. Finally, a highly polymorphic p53 CA repeat was used for detection of LOH that allowed us to analyze nearly 90% of our samples. Our results show that frequent p53 mutation and allelic loss are associated with both low- (LOH, 6.8%; mutation, 18.8%) and high- (LOH, 28.6%; mutation, 33.3%) grade MALT lymphomas, particularly in the high-grade tumors. Our data also indicate that there is a significant difference in the extent of p53 abnormalities between low- and highgrade MALT lymphomas. In low-grade tumours, most p53defective cases (10/1 l ) showed either one allele mutation or one allele loss, suggesting only partial loss of p53 function. Whereas in high-grade tumors, the majority (6/9) of affected cases exhibited both p53 mutation and allele loss, implying complete loss of p53 function. Thus, the extent of p53 inactivation is closely associated with the progression of MALT lymphoma. A partial inactivation of p53 function may be involved in the development of some low-grade MALT lymphomas, whereas a complete loss of p53 function may be important for high-grade transformation. In this aspect, the pathologic role of p53 inactivation in MALT lymphoma is different from that in follicular lymphoma in which p53 mutation has been suggested to be mainly associated with high-grade transformation” or that in splenic marginal zone B-cell lymphoma in which p53 inactivation appears to be involved with the development of low-grade tumors.” The observation of distinct p53 gene abnormalities at the different stages of MALT lymphoma shows the nature of multistage tumorigenesis and highlights the fact that the loss of normal p53 function is a process of the accumulation of its genetic abnormalities. Our results also emphasizes the importance of analyzing both p53 mutation and allele loss to evaluate its full potential in tumorigenesis. Distinct p53 gene abnormalities (p53 allele loss, p53 mutation, or both) may cause different degree of p53 inactivation, exert different tumorigenic potentials, and thus play different roles during multistage tumorigenesis. Mutant p53 protein frequently accumulates in cell nuclei due to its increased half-life and can be detected by immunochemical ~taining.”.~’ p53 staining frequently indicates mutation of the gene. In the present study, all cases showing p53 staining contained missense mutations in the gene. However, 14 cases harboring p53 mutation did not show any p53 459 1 staining with CM1 antibody. Staining was not expected for 5 cases, including 2 cases in which the mutation was silent and another 3 cases in which mutations resulted in truncated protein products. The mechanism underlying the absence of p53 staining in the remaining 9 cases, in which missense mutations were found, is unclear. Lack of staining in tumors harboring p53 missense mutation has been reported prev i o u ~ l y . ”Two ~,~~ possibilities may account for this. First, the accumulation of mutant p53 may not reach the level detectable by immunochemistry. This could be due to the insufficient stabilization of some mutations or lack of sufficient protein expression?2.MSecondly, the antibody used may not recognize all mutant p53. However, this possibility is small in our case because p53 staining was performed using CM1 and antigen retrieval method, which together have been shown to be highly sensitive for the ~taining.~’ In addition, the CM1 antibody is polyclonal and theoretically should recognize all p53. Nevertheless, the use of immunostaining with CM1 antibody alone as a marker for p53 mutation in MALT lymphomas is limited. Increasing expression of mutant p53 during tumor progression has been observed previously?* This suggests that additional mechanisms other than gene mutation may affect the protein expressionM and the expression of mutant p53 may also be functionally important. Increasing evidence suggests that mutant p53 can exert dominant negative effects on the ~ i 1 d - t y p e . l Thus, ~ ~ ~ ’ the lack of mutant p53 expression in tumors harboring p53 mutation may indicate the absence of such dominant negative effects and represent a relatively weak model of mutation-mediated p53 inactivation. In keeping with this hypothesis, the majority of our cases not showing protein accumulation but with mutation were low grade and did notshow loss of the other allele. Further studies following-up p53 expression and disease progression in these cases are necessary to understand the significance of mutant p53 expression in tumorigenesis. Analysis of the p53 mutation spectrum in a tumor may show the possible etiology involved in its carcinogene~is.’.~~ For example, the high frequency of G+T transversion in the codon 249 in human hepatocellular carcinoma is related to alfatoxin B 1-mediated mutagenesis,4’ whereas the predominant transition mutation in the CpG island observed in colon cancer and lymphomas suggests the endogenous mutagenesis4’ No predominant type of mutation in the p53 gene was found in MALT lymphoma and the mechanism underlying the diverse p53 mutations in this tumor remains unclear. Oxygen-reactive species, which are actively produced during inflammatory diseases49and cause various types of mutation in DNA:’ may be important in the carcinogenesis of MALT lymphoma because this tumor is frequently derived from a background of inflammatory diseases. Despite the progress in the understanding of clinical, pathologic, and immunophenotypic features of MALT lymphoma, the genetic mechanism underlying its development and high-grade transformation is not fully understood. This tumor does not show bcl-2 or bcl-l rea~~angement.’~,~~ Although c-myc rearrangement has been reported in 1 of 2 high-grade MALT tumors,53we have been unable to detect From www.bloodjournal.org by guest on January 26, 2015. For personal use only. 4592 DU ET AL c-mycrearrangement in arelativelylargeseriesofwelldefined high-grade MALT cases (our unpublished results). Recently, a high frequency (56%) of chromosome trisomy 3 hasbeen found in low-gradeMALT lymphoma,which indicates its important rolein the developmentof the tumor.54 However, trisomy of chromosome 3 does not appear to be important for the high-grade transformation because its frequency is lower(33%) in high-grade tumors.” Thus, the finding of p53 inactivation in MALT lymphoma represents the first gene identified so far to be frequently involved in its development as well as high-grade transformation. REFERENCES 1. Isaacson P, Wright DH: Malignant lymphoma of mucosa-asso- ciated lymphoid tissue. A distinctive type of B-cell lymphoma. Cancer 52:1410, 1983 2. Isaacson P, Spencer J: Malignant lymphoma of mucosa-associated lymphoid tissue. Histopathology 11:445, 1987 3. lsaacson PG: Lymphomas of mucosa-associated lymphoid tissue (MALT). Histopathology 16:617, 1990 4. 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For personal use only. 1995 86: 4587-4593 The accumulation of p53 abnormalities is associated with progression of mucosa-associated lymphoid tissue lymphoma M Du, H Peng, N Singh, PG Isaacson and L Pan Updated information and services can be found at: http://www.bloodjournal.org/content/86/12/4587.full.html Articles on similar topics can be found in the following Blood collections Information about reproducing this article in parts or in its entirety may be found online at: http://www.bloodjournal.org/site/misc/rights.xhtml#repub_requests Information about ordering reprints may be found online at: http://www.bloodjournal.org/site/misc/rights.xhtml#reprints Information about subscriptions and ASH membership may be found online at: http://www.bloodjournal.org/site/subscriptions/index.xhtml Blood (print ISSN 0006-4971, online ISSN 1528-0020), is published weekly by the American Society of Hematology, 2021 L St, NW, Suite 900, Washington DC 20036. 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