Biothermodynamics Prof. Dr.-Ing. habil. Mirjana Minceva Master thesis/ Bachelor thesis/ Forschungspraktikum Model based Development of a new method for multicomponent separation of biomolecules by liquid-liquid Chromatography Liquid-liquid chromatography is a new separation technology for isolation, purification and separation of natural products. The focus here is on pharmaceutical components, phytopharmaceuticals, plant extracts, flavourings, fragrances and pigments for the use in pharmaceutical industry, food- and beverage industry, and cosmetic industry. In liquid-liquid chromatography, systems with two liquid phases in equilibrium are used to separate target substances from a mixture. In our group we study the field of sequential centrifugal partition chromatography, sCPC, a new continuous liquid-liquid chromatographic process. In sCPC unit, the feed mixture is introduced continuously and the products are alternately collected. The objective of this work is to extend the development of a new method for the separation of multicomponent mixtures. Therefore, the influence of different process parameters has to be investigated with model mixtures and characterized for industrial application. Methods and devices that will be used include: - Centrifugal partition chromatography (CPC/sCPC) - High Performance Liquid Chromatography (HPLC) - UV-vis Spectroscopy Figure: sCPC – set-up (model TMB-250 Armen Instrument, France) Requirements: You should be interested in separation processes and have experience in laboratory work. You will get the chance to acquire theoretical and practical knowledge about preparative liquid chromatography. Start: April 2015 Simon Röhrer Gregor-Mendel Straße 4, 1. OG 04 (iGZW) Tel.: 08161.71.6167 E-Mail: [email protected]