GE Healthcare
Life Sciences
illustra™ Nucleic Acid Sample Preparation
Purify. Amplify. Simplify.
Get attached with illustra
nucleic acid purification
illustra product range includes fast, efficient solutions for nucleic acid
purification and amplification. From our illustra DNA sample prep kits to
our illustra RNAspin kits for RNA purification, and illustra HotStart RTG™
and Master Mix for PCR, illustra delivers high-quality results with excellent
purity and reproducibility.
And illustra is easy to use, with simplified protocols that integrate colorcoded kit components, pre-equilibrated, columns, and minimal pipetting
volume changes. Whatever your sample source, desired sample type,
or downstream application, illustra makes nucleic acid purification and
amplification a snap.
www.vwr.com
Selection guide for nucleic acid sample preparation
Nucleic Acid Workflow
Genome and Gene Analysis
Sample(s): blood, tissue, cells, bacteria, yeast, etc.
Plasmid DNA Preparation
RNA Preparation
Plasmid DNA Purification
Plasmid Amplification
Genomic DNA Preparation
Genomic DNA Purification
RNA Purification
Whole Genome Amplification
PCR
RT-PCR
PCR
DNA Cleanup
DNA Cleanup
DNA Cleanup
Cloning & Sequencing
Gene Expression
Genotyping
or
Cloning & Sequencing
Biomolecule of Interest: e.g., Biomarker
lasmid
Genomic DNA, total RNA, and total denatured protein purification
NEW illustra triplePrep™ Kit
illustra triplePrep Kit is designed for rapid, simultaneous extraction
and isolation of high quality genomic DNA (gDNA), total RNA and total
dentured proteins from animal tissues and mammalian cells. High yields
of quality DNA, RNA, and proteins can be extracted in less than 1 hour
using a flexible, easy-to-follow workflow allowing researchers to directly
correlate data generated from the same sample.
The isolated DNA, total RNA, and total dentured proteins are suitable for
genomic and proteomic applications such as PCR, restriction enzyme
digestion, sequencing, array CGH, RT-PCR, gene expression microarray,
SDS-PAGE, Western blotting, 2-D DIGE, and LCMS.
Fast and simple. From sample to DNA/RNA/protein in
less than 1 hour - streamlined workflow reduces the overall
number of steps, resulting in up to 70% time saving compared
to preparing each analyte individually.
High yield. High DNA, RNA, and protein yields from small
samples—optimized buffer, columns, and protocol ensure high
recovery of gDNA, total RNA, and total denatured proteins.
Easy to use
• Flexible workflow. can isolate any two or all three analytes with multiple stop points in the protcol.
•User friendly. minimal change of centrifugation speed,
time and pipetting volume.
High quality.
• High purity—DNA-free RNA, with DNase provided in the kit.
• Suitable for downstream applications—gDNA, RNA, and
proteins validated in numerous downstream applications.
•Easy to follow. color-coded caps and bottles with matching protocol steps minimize that chance for error. A quick
refernce protocol card provides instructions at a glance for
experienced users.
Typical yields and purities from different tissues
Tissue
Liver
Brain
Heart
Kidney
Lung
Spleen
Tissue input amount (mg)
Disruption difficulty
Need extra spin
10
Easy
No
10
Easy
No
10
Hard
No
10
Medium
No
10
Medium
Yes
5
Medium
No
DNA
Yield (μg)
Purity (A260/A280)
14.6
1.9
6.8
1.8
7.4
1.9
22.4
1.9
15.5
1.8
18.8
1.8
RNA
Yield (μg)
Purity (A260/A280)
Need DNase
44
2.1
Yes
5.5
2.0
No
4.0
2.0
No
8.1
2.1
Yes
8.8
2.0
Yes
18.9
2.1
Yes
Protein
Yield (μg)
1460
746
923
897
592
510
Note: Yields and purities of gDNA, total RNA, and total denatured proteins can vary with user and depend on the nature and condition of the input sample.
Typical yields and purities from different cells
Cell
Cell input
amount (millions)
4
HeLa
NIH-3T3
CHO-K1
HEK-293
1
1
1
1
DNA
Yield (µg)
Purity (A260/A280)
8.5
1.9
9.5
1.9
4.4
1.9
9.1
1.9
RNA
Yield (µg)
Purity (A260/A260)
13.7
2.1
7.9
2.1
6.6
2.0
10.1
2.0
Protein
Yield (µg)
139
127
77
139
Ordering information
Product
Quantity
VWR Cat. No.
illustra triplePrep Kit
50 preps
95056-354
Whole genome amplification (WGA)
illustra GenomiPhi™ DNA Amplification Kits
Representative whole genome amplification (WGA)
illustra GenomiPhi provides an easy way to prepare or
replenish genomic DNA from limiting stocks or source
materials. With as little as 10 ng of starting DNA, GenomiPhi
can generate µg amounts of amplified DNA using a simple
enzymatic reaction. Reactions are easily automated and
scalable. Amplified DNA is suitable for use in a variety of
downstream genotyping and cloning applications.
illustra GenomiPhi V2 DNA Amplification Kit
GenomiPhi V2
Quick mini-scale genomic DNA preparation: 4 - 7 μg in less
than 2 hours. No background amplification generated in
negative (no-template) control reactions.
illustra GenomiPhi HY DNA Amplification Kit
Midi-scale genomic DNA preparation: 40 - 50 μg in less than 4
hours.
GenomiPhi HY
GenomiPhi V2
7
GenomiPhi HY
6
60
10 ng gDNA
NTC
10 ng gDNA
NTC
50
4
40
Yield (µg)
Yield (µg)
5
3
2
30
20
10
1
0
0
0
0.5
1
Amplification time (h)
1.5
2
0
1
2
3
4
Amplification time (h)
Yield vs time plot for two GenomiPhi kits
NTC: No template control; 10 ng DNA: 10ng purified genomic DNA was used as starting material.
5
Whole genome amplification (WGA)
Success rate (%)
illustra GenomiPhi DNA Amplification Kits
GenomiPhiProtocol
GenomiPhi
73
72
Input DNA (ng)
71
Sample buffer
70
69
68
GenomiPhi
Number of
wrong calls
gDNA
6
5
4
3
2
1
0
REPLI-g
™
GenomePlex
Heat or chemically denature.
Reaction buffer
Enzyme mix
GenomiPhi
REPLI-g
GenomePlex
Incubate at 30˚C.
Inactivate enzyme at 65˚C.
Representative amplification with illustra GenomiPhi DNA Amplification Kit
Human genomic DNA samples were amplified in triplicate using various DNA
amplification methods, followed by genotyping for over 5000 SNPs using the
Linkage Panel IV chip performed by Illumina™ Genotyping Service. Amplified
DNA produced with illustra GenomiPhi DNA Amplification Kit was 100%
concordant with genomic DNA.
Ordering information
6
Product (µg)
Ordering information
Product
Quantity
VWR Cat. No.
Product
Quantity
VWR Cat. No.
illustra GenomiPhi V2
DNA Amplification Kit
25 reactions
95042-266
illustra GenomiPhi HY
DNA Amplification Kit
25 reactions
95042-268
illustra GenomiPhi V2
DNA Amplification Kit
100 reactions
95040-352
illustra GenomiPhi HY
DNA Amplification Kit
100 reactions
95040-348
illustra GenomiPhi V2
DNA Amplification Kit
500 reactions
95040-354
illustra GenomiPhi HY
DNA Amplification Kit
1000 reactions
95040-350
Plasmid DNA sequencing template preparation
illustra TempliPhi™ DNA Amplification Kits
Culture-free plasmid DNA preparation – straight from
the plate
Prepare DNA directly from bacterial colonies or glycerol stocks.
Eliminates overnight cultures and conventional plasmid
mini preps. Use amplified DNA directly in downstream DNA
sequencing without further purification.
illustra TempliPhi 100/500 DNA Amplification Kit
• For low to medium throughput, small DNA vector
preparation (e.g. pUC and M13).
illustra TempliPhi 100 DNA Amplification Kit
• High-quality DNA in as little as 4 hours.
• Minimal hands-on time - easy to automate.
illustra TempliPhi 2,000 Reaction Kit
illustra TempliPhi Large Construct DNA
Amplification Kit
For higher-throughput sequencing template preparation.
For large DNA vector preparation (e.g. fosmid and BAC).
High quality DNA generates accurate
sequencing results
Plasmid DNA template was amplified with illustra
TempliPhi 100 Amplification Kit and subsequently
sequenced using DYEnamic™ ET Terminator Cycle
Sequencing Kit and analyzed on an ABI™ PRISM™
3100 Genetic Analyzer.
Ordering information
Product
Quantity
VWR Cat. No.
illustra TempliPhi
100 reactions
89131-608
illustra TempliPhi
500 reactions
89131-612
illustra TempliPhi DNA
Sequence Template
Preparation Kit
2,000 reactions
89134-006
Plasmid DNA amplification using illustra TempliPhi DNA amplification kit
7
Difficult-to- sequence plasmid DNA templates
illustra TempliPhi Sequence Resolver Kit
Sequence resolution with a single kit — Solve the most
common sequencing problems (repeats, sequencing stops,
compressions) with one kit rather than multiple methods and
reagents.
Simple
•Culture-less preparation—Amplify DNA from bacterial
colonies, glycerol stocks, or purified DNA, saving costs in
time, labor, and reagents.
•Compatible with a variety of sequencing chemistries,
including DYEnamic ET terminator and BigDye™ v3.1
terminator cycle sequencing kits.
•Can be easily deployed upstream of standard sequencing
protocols and instruments.
High Quality
Fast
•More complete, accurate sequences—Improve difficult
template sequencing success with up to 820 bp Phred 20
read lengths.
•Just 15 min total hands-on time—Implement a simplified
protocol with fewer steps and no overnight culturing.
•No purification required—Use prepared DNA directly in
sequencing reactions, eliminating purification steps to
achieve higher sequencing throughput.
Comparative resolution of GC-rich region with dGTP BigDye v3.0 and
illustra TempliPhi Sequence Resolver Kit.
Plasmid template (1 μl) was amplified using either illustra TempliPhi 100
Amplification Kit or illustra TempliPhi Sequence Resolver Kit. Amplified
product (400 ng) was sequenced with either the dGTP BigDye kit (illustra
TempliPhi 100 product) or the DYEnamic ET Terminator kit (TempliPhi
Sequence Resolver product) per the standard protocols. Sequencing
reactions were analyzed on an ABI 3730xl DNA Analyzer. Note the
GC-rich region toward the end of the sequence is poorly resolved with
the dGTP technique, resulting in miscalling. illustra TempliPhi Sequence
Resolver Kit resolves compressions present with the dGTP technique.
8
Ordering information
Product
Quantity
VWR Cat. No.
illustra TempliPhi Sequence
Resolver Kit
50 reactions
89131-616
illustra TempliPhi Sequence
Resolver Kit
200 reactions
89131-618
Genomic DNA purification
When your sample source is cell cultures or tissue, illustra tissue and
cells genomicPrep™ kits provide fast extraction and purification of
high-quality genomic DNA. illustra tissue and cells genomicPrep Mini
Spin Kit allows you to go from sample to genomic DNA in just 90 min.
illustra tissue and cells genomicPrep Midi Flow Kit reduces protocol
time, including homogenization, by more than 2 h, as compared with
other commercially available kits.
illustra tissue and cells genomicPrep Mini Spin Kit
Technical specifications
For the rapid extraction and purification of high-quality
genomic DNA from a variety of animal tissues and mammalian
cell cultures.
tissue & cells Mini Spin Kit (5 to 50 mg of Rat Liver; 20 mg optimum)
Ordering information
Yield
Purity
12.0 to 25.0 μg
1.75 to 1.90
Total time of prep*
90 min
Hands-on time*
20 min
Product
Quantity
VWR Cat. No.
Size
illustra tissue and cells
genomicPrep Mini Spin Kit
50 preps
95026-754
*prep time does not include homogenization
illustra tissue and cells
genomicPrep Mini Spin Kit
250 preps
20 to 35 kb
Tissue & cells Mini Spin ( 3 × 105 - 5 × 106 cells)
95026-756
Yield
13 to 17 μg
Purity
1.75 to 1.90
Total time of prep*
Hands-on time*
Size
70 min
20 min
up to 75 kb
*Cell type dependent, CHO and HK293 cells used in testing
illustra tissue and cells genomicPrep Midi Flow Kit
Technical specifications
For the high-yield extraction and purification of high-quality
genomic DNA from tissues and cells, 20% higher yield than
other commercially available kits with an average size of
> 20 kb.
tissue & cells Midi Flow Kit (50 to 200 mg of tissue; 100 mg optimum)
Ordering information
Yield
Purity
100 to 135 μg
1.7 to 1.9
Total time of prep*
3h
Hands-on time*
1h
Size
Product
Quantity
VWR Cat. No.
*prep time does not include homogenization
illustra tissue and cells
genomicPrep Midi Flow Kit
25 preps
95026-752
Tissue & cells Midi (1 × 107 to 2 × 107; 2 × 107 optimum)
Yield
Purity
Total time of prep*
Hands-on time*
Size
> 50 kb
100 to 135 μg
1.7 to 1.9
3h
1h
> 50 kb
*including lysis, purification & desalting
9
Genomic DNA purification
illustra blood genomicPrep Mini Spin Kit
For the rapid and reproducible isolation of high-quality
genomic DNA from whole blood, buffy coat, bone marrow,
and nucleated red blood cells.
Ordering information
Product
Quantity
VWR Cat. No.
illustra blood genomicPrep
Mini Spin Kit
50 preps 95026-740
illustra blood genomicPrep
Mini Spin Kit
250 preps 95026-742
Technical specifications
Yield
4 to 6 µg/200 µl whole blood
Purity
> 1.6
Total time of prep*
less than 20 min
Hands-on time*
illustra blood genomicPrep Mini Spin Kit
less than 5 min
Size
> 20 kbp
*Including lysis, purification, and desalting
illustra blood genomicPrep Midi Flow Kit
For the rapid extraction and purification of high-quality
genomic DNA from whole blood, buffy coat, and
animal blood.
Ordering information
Product
Quantity
illustra blood
25 preps genomicPrep Midi Flow Kit
VWR Cat. No.
95026-736
illustra bacteria genomicPrep Mini Spin Kit
For the rapid extraction and purification of high-quality
genomic DNA from Gram-positive and Gram-negative bacteria.
Technical specifications
blood Midi Flow Kit (1 to 8 ml; 5 ml optimum)
Yield
85 to 120 μg
Purity
1.7 to 1.9
Total time of prep*
2h
Hands-on time*
45 min
Size
> 50 kb
*including lysis, purification & desalting
Technical specifications
bacteria Mini Spin Kit (1 × 109 to 4 × 109 cells)
Yield
4 to 12 μg
Purity
1.7 to 1.9
Ordering information
Total time of prep*- (Gram-negative)
40 min
Product
Quantity
VWR Cat. No.
Hands-on time*
15 min
illustra bacteria
genomicPrep Mini Spin Kit
50 preps 95026-732
Total time of prep*- (Gram-positive)
55 min
illustra bacteria
genomicPrep Mini Spin Kit
250 preps 10
95026-734
Hands-on time*
15 min
Size
> 20 kb
*includes testing on a wide range of strains
Genomic DNA purification
Nucleon™ BACC Genomic DNA Extraction Kits
Nucleon PhytoPure™ Genomic DNA Extraction Kits
Developed for rapid extraction of high-quality, high-molecular- PhytoPure resin binds plant polysaccharides ensuring high
weight genomic DNA from blood and cell cultures.
yields of high-quality plant genomic DNA in only one hour.
Ordering information
Ordering information
Product
Quantity
VWR Cat. No.
Product
Quantity
VWR Cat. No.
Nucleon BACC1
1 kit
95017-484
Nucleon PhytoPure
50 preps × 0.1 g
95026-702
Nucleon BACC2
1 kit
95026-698
Nucleon PhytoPure
50 preps × 1.0 g
95026-704
Nucleon BACC3
1 kit
95017-486
Nucleon HT Genomic DNA Extraction Kit
Technical specifications
Nucleon BACC1: 50 preps of 1 ml of whole blood or
cultured cells (1 to 3 × 106 cells/ml)
Nucleon BACC2:
50 preps of 10 ml of whole blood or
cultured cells (3 to 10 × 106 cells/10 ml) or
100 preps of 1 ml (1 to 3 × 106 cells/ml)
Nucleon BACC3: 50 preps of 10 ml of whole blood
Facilitates the simple, phenol-free extraction of genomic
DNA from paraffin-embedded sections and hard tissue
requiring proteinase K digestion.
Ordering information
Product
Quantity
VWR Cat. No.
Nucleon HT
1 kit, 50 preps
95026-700
Plasmid DNA purification
illustra plasmidPrep Mini Spin Kit provides rapid isolation of highand low-copy number plasmid DNA for use in cloning, restriction
enzyme digestion, PCR amplification, and DNA sequencing. For
medium-scale cultures, illustra plasmidPrep Midi Flow Kit is ideal
for transfection, sequencing, and enzymatic amplification and
modification applications.
illustra plasmidPrep Mini Spin Kit
Technical specifications
For the rapid isolation of high and low copy number
plasmid DNA.
plasmidPrep Mini (1.5 ml; A600 = 2.5 to 3.0)
Yield
Purity A260/A280
Purity A260/A230
Ordering information
Product
Quantity
VWR Cat. No.
illustra plasmidPrep Mini
Spin
50 preps 95026-748
illustra plasmidPrep Mini
Spin
250 preps 6 to 9 μg
> 1.8
> 1.7
Total time of prep
~ 9 min
Hands-on time
~ 3 min
Size
6.3 kb
plasmidPrep Mini (3.0 ml; A600 = 2.5 to 3.0)
95026-750
Yield
Purity A260/A280
Purity A260/A230
9 to 15 μg
> 1.8
> 1.7
Total time of prep
~ 9 min
Hands-on time
~ 3 min
Size
6.3 kb
11
Plasmid DNA purification
illustra plasmidPrep Midi Flow Kit
Technical specifications
For the rapid extraction and purification of high-quality
plasmid DNA from medium scale cultures for use in
transfection, sequencing, and enzymatic amplification
and modification.
Ordering informationon
Product
Quantity
VWR Cat. No.
illustra plasmidPrep Midi Flow
25 preps
95026-744
Yield, purity, and quality values obtained with high- and low-copy
number plasmid DNA purified with the plasmidPrep Midi Flow Kit.
Plasmid copy number
High
Low
Plasmid size (kb)
6.3
36
Culture volume (ml)
50
150
Overall plasmid DNA yield (μg)
289
191
Endotoxin (EU/μg pDNA)
0.05
0.13
A260/A280 ratio
1.90
1.93
A260/A230 ratio
2.34
2.29
Supercoiled momomer (%)
91.5
ND
Transfection (% COS7 cells)
70
ND
Sequencing (Phred20 read length)
818
ND
plasmidPrep Midi (25 to 150 ml culture)
Effective capacity Yield 250 μg
100 to 250 μg high-copy number plasmid;
20 to 250 μg low- and very-low-copy number plasmid
% supercoiled pDNA endotoxin Total time of prep Hands-on time Size illustra plasmidPrep Midi Flow Kit
Size
Sephacryl™ S-1000
For large-scale purification of plasmid DNA by gel filtration.
Ordering information
Product
Quantity
VWR Cat. No.
Sephacryl S-1000 SF
750 ml
95016-856
RNA purification
Simple, convenient, and suitable for all levels of expertise, illustra
RNAspin Mini Isolation Kit recovers high-quality total RNA from diverse
sample types—even when your sample size is very small (e.g., 10 HeLa
cells for RT-PCR). For applications requiring high yields, use illustra
RNAspin Midi Isolation Kit with scalable input and output. illustra
RNAspin 96 RNA Isolation Kit provides fast, efficient purification in a
96-well format, with flexible processing by vacuum or centrifugation.
All illustra RNAspin kits can be used with cultured cells, tissue,
bacteria, or yeast samples.
12
> 70% (strain dependent)
< 10 EU per μg plasmid DNA
~ 215 min
~ 40 min
2 to > 50 kb
RNA purification
illustra RNAspin™ Mini Isolation Kit
High-quality output RNA from diverse sample types; suitable
for use in sensitive downstream applications.
Ordering information
Product
Quantity
VWR Cat. No.
illustra RNAspin Mini Kit
20 preps
95017-489
illustra RNAspin Mini Kit
50 preps
95017-491
illustra RNAspin Mini Kit
250 preps
95017-493
Technical specifications
Animal Tissue
Cell Culture
Sample size up to 30 mg tissue up to 5 × 106 cells
Typical yield up to 70 μg up to 70 μg
Elution volume 40–120 μl
Effective binding capacity
RNA integrity 100 μg
sharp rRNA bands with no substantial
degradative bands visible 28S:18S = ~ 2:1
RNA Integrity Number (RIN) values 7
RNA purity Total time of prep illustra RNAspin Mini Isolation Kit
A260/A280 = 1.8–2.2
< 30 min/6 preps
illustra RNAspin Midi Isolation Kit
Technical specifications
For applications requiring high yields.
Ordering information
Animal Tissue Cell Culture
Sample size up to 200 mg tissue up to 5 × 107 cells
Typical yield up to 700 μg up to 700 μg
Product
Quantity
VWR Cat. No.
Elution volume 500 μl
illustra RNAspin Midi Kit
20 preps
95017-495
Effective binding capacity
700 μg
RNA integrity sharp rRNA bands with no substantial
degradative bands visible 28S:18S = ~ 2:1
RNA Integrity Number (RIN) values 7
A260/A280 = 1.8–2.2
RNA purity Total time of prep illustra RNAspin 96 RNA Isolation Kit
Technical specifications
For fast and efficient purification in a 96-well format.
Ordering information
Product
80 min/4 preps
Animal Tissue
Sample size Quantity
VWR Cat. No.
Typical yield Cell Culture
Vacuum Centrifuge Vacuum Centrifuge
10–30 mg 30 mg tissue 2 × 106
1 × 107
up to 40 μg up to 100 μg up to 20 μg up to 100 μg
illustra RNAspin 96 Kit
4 × 96 preps
95017-499
Elution volume illustra RNAspin 96 Kit
24 × 96 preps
95017-501
Effective binding capacity RNA integrity RNA purity Total time of prep 50–130 μl
100 μg
sharp rRNA bands with no substantial
degradative bands visible 28S:18S = ~ 2:1
RNA Integrity Number (RIN) values 7
A260/A280 = 1.8–2.2
70 min/96 preps
13
RNA purification
illustra RNAspin 96 Filter Plates
Allows for tissue samples to be processed with the RNAspin.
illustra QuickPrep™ Micro mRNA Purification Kit/
illustra QuickPrep mRNA Purification Kit
For rapid purification of mRNA directly from a wide variety of
eukaryotic cells or tissue samples, even from a single cell.
Ordering information
Product
Quantity
VWR Cat. No.
illustra RNAspin 96 Filter
Plates
4
95026-706
illustra Cesium Trifluoroacetate (CsTFA)
For separation and purification of nucleic acids, especially
RNA, by isopycnic centrifugation.
Ordering information
Product
Quantity VWR Cat. No.
illustra QuickPrep Micro mRNA
Purification Kit
1 kit
95017-636
illustra QuickPrep mRNA
Purification Kit (4 purifications)
1 kit
95017-634
illustra mRNA Purification Kit
Ordering information
Product
Quantity
VWR Cat. No.
illustra CsTFA (Solution)
100 ml
95016-998
For rapid affinity purification of mRNA from eukaryotic total
RNA using prepacked oligo(dT)-cellulose spun columns.
Ordering information
Oligo(dT)-Cellulose
Product
Quantity
VWR Cat. No.
For simple, affinity-purification of polyadenylated mRNA.
illustra mRNA Purification Kit
4 purifications (8 columns)
1 kit
95017-638
Ordering information
Product
Quantity
VWR Cat. No.
Oligo(dT)-Cellulose Type 7 1 g
95026-714
Oligo(dT)-Cellulose Type 7 5 g
95026-716
DNA Clean-up
GE Healthcare provides a wide range of nucleic acid purification
products for DNA clean-up. Whether your application needs require
purification of PCR products and restriction fragments; removal of
dye terminators, unincorporated labels and nucleotides; or desalting,
illustra DNA clean-up products have been optimized for high
performance in your specific application. The products have also been
designed for convenience and speed with prepacked columns as well
as quick reference protocol cards for ease of use. Many of the kits are
tailored for a specific application to ensure purification of the highest
quality DNA.
illustra GFX™ PCR DNA and Gel Band Purification Kit
Ordering information
For the isolation and concentration of DNA fragments (50 bp
to 10 kb) from PCR mixtures, DNA-containing agarose gel
bands, enzyme-based DNA modifications, and restriction
digestions.
Product
Quantity
VWR Cat. No.
illustra GFX PCR DNA and
Gel Band Purification kit
100
purifications
95026-728
illustra GFX PCR DNA and
Gel Band Purification kit
250
purifications
95026-730
14
DNA Clean-up
illustra GFX 96 PCR Purification Kit
For the purification of up to 96 PCR products (0.1 to 10 kb)
simultaneously in as little as 15 min.
Ordering information
Product
Quantity
VWR Cat. No.
illustra GFX 96 PCR Purification
kit
10 × 96 well
plates
95026-726
Technical specifications
Protocol Elution volume (μl) Yield (%)
Centrifugation 10 55 100 55
95
90
Vacuum 50 100 59
78
illustra GFX 96 PCR Purification Kit
ExoSAP-IT™
illustra MicroSpin S-300 HR Columns
For rapid and efficient purification of PCR products.
For rapid purification of PCR products ( 100 bp) from
unincorporated primers ( 20-mers) and nucleotides using
spin-column chromatography.
Ordering information
Product
Quantity
VWR Cat. No.
ExoSAP-IT
100 reactions
95026-708
Ordering information
ExoSAP-IT
500 reactions
95026-710
Product
Quantity
VWR Cat. No.
ExoSAP-IT
2000 reactions
95026-712
50
95017-617
ExoSAP-IT
5000 reactions
95040-358
illustra MicroSpin S-300 HR
Columns
illustra MicroSpin™ S-200 HR Columns
For rapid purification of labeled single-stranded or doublestranded DNA fragments 50 bases in length in a volume of
51 to 100 μl using spin-column chromatography.
Ordering information
Product
Quantity
VWR Cat. No.
illustra MicroSpin S-200 HR
Columns
50
95017-615
illustra MicroSpin S-300 HR Columns
illustra MicroSpin S-400 HR Columns
For rapid purification of PCR products ( 200 bp) from
unincorporated primers ( 32-mers) and nucleotides using
spin-column chromatography.
Ordering information
Product
Quantity
VWR Cat. No.
illustra MicroSpin S-400 HR
Columns
50
95017-619
illustra MicroSpin S-200 HR Columns
15
DNA Clean-up
Ordering information
illustra MicroSpin G-25 Columns
Product
Quantity
VWR Cat. No.
illustra MicroSpin G-25
Columns
50
95017-621
Technical specifications
Volume* % Average yield† Standard deviation Average purity‡ Standard deviation *
†
‡
illustra MicroSpin G-25 Columns
100 μl 150 μl
83% 5.1
98% 2.9 89%
3.6
97%
4.8
A 17-mer was synthesized on a 0.2 μm scale and deprotected at 60°C for 30 min in
concentrated ammonium hydroxide. 100 μl or 150 μl of deprotected oligo was applied
to pre-spun MicroSpin G-25 Columns and centrifuged at 735 × g for 2 min in a
microcentrifuge. As a standard, 0.25 ml of deprotected oligo was diluted to 1 ml and
purified on a NAP-10 column according to instructions provided with the columns.
Yield is based on A260 reading following purification. Yield from NAP-10 Column is a
standard to which we have assigned 100% yield.
Purity is based on amount of ammonia present following purification as determined
using Nessler reagent. Purity from NAP-10 Column is a standard to which we have
assigned 100% purity.
illustra MicroSpin G-50 Columns
illustra NICK™ Columns
For rapid purification of labeled DNA ( 20 bases) from
unincorporated labeled nucleotides in a volume of 25 to 50 μl
using spin-column chromatography.
For rapid purification of labeled DNA ( 20 bases in length)
from unincorporated radiolabeled nucleotides using
gravity-flow chromatography.
Ordering information
Ordering information
Product
Quantity
VWR Cat. No.
Product
Quantity
VWR Cat. No.
illustra MicroSpin G-50
Columns
50
95017-623
illustra NICK Columns
20
95017-015
illustra MicroSpin G-50
Columns
250
95017-625
illustra NICK Columns
50
95017-017
illustra ProbeQuant™ G-50 Micro Columns
illustra AutoSeq™ G-50
For rapid removal of unincorporated fluorescent dye-terminators
from cycle sequencing reactions prior to automated sequencing.
For rapid purification of labeled DNA ( 20 bases) from
unincorporated labeled nucleotides in a volume of 25 to 50 μl
using spin-column chromatography.
Ordering information
Ordering information
Product
Quantity
VWR Cat. No.
illustra AutoSeq G-50
50 columns
95017-629
illustra AutoSeq G-50
250 columns
95017-631
illustra AutoSeq G-50
1000 columns
95017-633
Product
Quantity
VWR Cat. No.
illustra ProbeQuant G-50
Micro Columns
50
95026-724
illustra ProbeQuant
G-50 Micro Columns
illustra AutoSeq G-50
16
DNA Clean-up
illustra NAP™ Columns
Technical specifications
For rapid and efficient purification of DNA and oligonucleotides
(≥ 10-mers) in less than 15 min by gravity flow.
Ordering informationrdering information
Choosing a NAP column and buffer volumes
Column Type
Sample
Volume (ml)
Equilibration
Buffer (ml)
Elution Buffer
(ml)
NAP-5
0.1
0.4
0.5
Product
Quantity
VWR Cat. No.
0.25
0.25
0.7
illustra NAP-5 Columns
20
95017-007
Max. vol.
0.5
0
1.0
illustra NAP-5 Columns
50
95017-009
NAP-10
0.75
0.25
1.2
illustra NAP-10 Columns
20
95017-011
Max. vol.
1.0
0
1.5
illustra NAP-10 Columns
50
95017-013
NAP-25
1.5
1.0
2.5
illustra NAP-25 Columns
20
95017-003
2.0
0.5
3.0
2.5
0
3.5
illustra NAP-25 Columns
50
Max. vol.
95017-005
illustra Sephadex™ G-25 DNA Grade
illustra CyScribe™ GFX Purification Kit
For purification of DNA (≥ 10 bases in length) from small
molecules by gel filtration.
Allows rapid purification of CyDye™ labeled cDNA probes.
Ordering information
Ordering informationdering information
Product
illustra Sephadex G-25 DNA
Grade SF
Quantity
100 g
Product
illustra CyScribe™ GFX
Purification Kit
illustra CyScribe GFX
Purification Kit
VWR Cat. No.
95016-894
Quantity
25
purifications
50
purifications
VWR Cat. No.
95026-722
95017-644
illustra Sephadex G-25 DNA Grade
illustra Sephadex G-50 and G-100 DNA Grade
For purification of DNA from small molecules by gel filtration.
Ordering informationon
Product
illustra Sephadex G-50 DNA
Grade F
illustra Sephadex G-50 DNA
Grade F
illustra Sephadex G-50 DNA
Grade M
Quantity
25 g
VWR Cat. No.
95016-896
100 g
95016-898
MicroSpin Columns
100 g
95016-782
Empty MicroSpin Columns for use with Sephadex DNA Grade
media, or any media.
illustra CyScribe GFX Purification Kit
Ordering information
Technical specifications
G-25 DNA exclusion limit (base pairs) 10
Oligonucleotide exclusion limit [poly (A)] 10 ml of hydrated gel per gram of dry medium 4-6
G-50
G-100
20
20 9-11
25
27
15-20
Product
MicroSpin Columns
Quantity
100
VWR Cat. No.
95017-597
17
GE, imagination at work, and GE monogram are trademarks of General Electric Company.
AutoSeq, CyDye, CyScribe, DYEnamic, genomicPrep, GenomiPhi, GFX, GraviTrap, GSTprep, GSTrap,
HiTrap, HisTrap, HisPrep, illustra, LabMate, MBPTrap, MicroSpin, MidiTrap, MiniTrap, MultiTrap,
NanoVue, NAP, NICK, Nucleon, PreScission, ProbeQuant, PuReTaq, QuickPrep, Ready-To-Go, RNA
Spin, Sephacryl, Sephadex, Sepharose, SpinTrap, StrepTrap, triplePrep, TempliPhi, and Whatman
are trademarks of GE Healthcare companies.
CyDye: This product, or portions thereof, is manufactured under an exclusive license from
Carnegie Mellon University under US patent number 5,268,486 and equivalent patents in the US
and other countries.
ExoSAP-IT: Exonuclease I/Shrimp Alkaline Phosphatase method of use is covered by one or more
of the following US patent numbers: 5,756,285 and 5,741,676 in the name of GE Healthcare BioSciences Corp.
Phi 29 DNA Polymerase: Phi 29 DNA polymerase and its use for DNA synthesis is covered by US
patent numbers 5,854,033, 5,198,543, 5,576,204 and 5,001,050.
Taq DNA Polymerase: This product is sold under licensing arrangements with Roche Molecular
Systems, F Hoffmann-La Roche Ltd and the Perkin-Elmer Corporation. Purchase of this product
is accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) process
for research in conjunction with a thermal cycler whose use in the automated performance of
the PCR process is covered by the upfront license fee, either by payment to Perkin-Elmer or as
purchased (i.e., an authorized thermal cycler).
FideliTaq is a trademark of USB Corporation. Illumina is a trademark of Illumina Inc. BigDye and
PRISM are trademarks of Applera Corporation. GenomePlex is a trademark of Rubicon Genomics
Inc. SEQUENOM and iPLEX are trademarks of Sequenom Inc. REPLI-g is a trademark of Qiagen.
FideliTaq DNA Polymerase technology is licensed under U.S. Patent 5,436,149 owned by Takara
Bio Inc.
2-D Protein Extraction Buffers, Mammalian Extraction Buffer and Yeast Extraction Buffer Kit are
manufactured by g-Biosciences, St Louis, MO, USA.Shinjuku-ku, Tokyo 169-0073, Japan
All third party trademarks are the property of their respective owners.
© 2009-2010 General Electric Company - All rights reserved.
First published May 2009.
1010 5M Lit. No. 07057REV
10/2010