1. No category

GE Healthcare
Selection guide for
protein and nucleic acid
sample preparation
Ordering information
illustra Nucleic Acid Purification & Amplification
Product
Genomic DNA Preparation
Genomic DNA Purification
illustra™ tissue and cells genomicPrep Mini Spin Kit
illustra tissue and cells genomicPrep Mini Spin Kit
illustra tissue and cells genomicPrep Midi Flow Kit
illustra blood genomicPrep Mini Spin Kit
illustra blood genomicPrep Mini Spin Kit
illustra blood genomicPrep Midi Flow Kit
illustra blood genomicPrep Midi Flow Kit
illustra bacteria genomicPrep Mini Spin Kit
illustra bacteria genomicPrep Mini Spin Kit
Nucleon™ BACC1 Genomic DNA Kit
Nucleon BACC2 Genomic DNA Kit
Nucleon BACC3 Genomic DNA Kit
Nucleon PhytoPure™
Nucleon PhytoPure
Nucleon HT (hard tissue)
Whole Genome Amplification
illustra GenomiPhi™ V2 DNA Amplification Kit
illustra GenomiPhi V2 DNA Amplification Kit
illustra GenomiPhi V2 DNA Amplification Kit
illustra GenomiPhi HY DNA Amplification Kit
illustra GenomiPhi HY DNA Amplification Kit
illustra GenomiPhi HY DNA Amplification Kit
Pack size
Code number
50
250
25
50
250
25
100
50
250
50
50
50
50 x 0.1g
50 x 1.0g
50
28-9042-75
28-9042-76
28-9042-73
28-9042-64
28-9042-65
28-9042-61
28-9042-62
28-9042-58
28-9042-59
RPN8501
RPN8502
RPN8512
RPN8510
RPN8511
RPN8509
25
100
500
25
100
1000
25-6600-30
25-6600-31
25-6600-32
25-6600-22
25-6600-20
25-6600-25
Plasmid DNA Preparation
Plasmid DNA Purification
illustra plasmidPrep Mini Spin Kit
50
illustra plasmidPrep Mini Spin Kit
250
illustra plasmidPrep Midi Flow Kit
25
illustra plasmidPrep Midi Flow Kit
100
750 ml
Sephacryl™ S-1000 SF
Yeast Plasmid Isolation Kit
50
Rolling Circle Amplification – Template Amplification
illustra TempliPhi™ 100 DNA Amplification Kit
100
illustra TempliPhi 500 DNA Amplification Kit
500
illustra TempliPhi Sequence Template Preparation Kit 10000
illustra TempliPhi Large Construct Kit
1000
illustra TempliPhi Sequence Resolver Kit
20 rxns
illustra TempliPhi Sequence Resolver Kit
50 rxns
illustra TempliPhi Sequence Resolver Kit
200 rxns
28-9042-69
28-9042-70
28-9042-67
28-9042-68
17-0476-01
US79220-50RXNS
25-6400-10
25-6400-50
25-6400-01
25-6400-80
28-9035-29
28-9035-30
28-9035-31
RNA Preparation
Total RNA
illustra RNAspin Mini Kit
illustra RNAspin Mini Kit
illustra RNAspin Mini Kit
illustra RNAspin Midi Kit
illustra RNAspin 96 Kit
illustra RNAspin 96 Kit
illustra RNAspin 96 Kit
illustra RNAspin 96 Filter Plate
illustra CsTFA (solution)
mRNA
illustra QuickPrep™ Micro mRNA Purification Kit
illustra QuickPrep mRNA Purification Kit
illustra mRNA Purification Kit
illustra mRNA Purification Kit
20
50
250
20
2 x 96
4 x 96
24 x 96
1
100 ml
25-0500-70
25-0500-71
25-0500-72
25-0500-73
25-0500-74
25-0500-75
25-0500-76
25-0500-88
17-0847-02
1
4
2
4
27-9255-01
27-9254-01
27-9258-01
27-9258-02
100 rxn
96 rxn
480 rxn
100
96 rxn plate
5 x 96 rxn plate
100 rxn tubes
96 rxn h-tube
100
100
50 units
250 units
1000 units
5 x 250 units
5000 units
250 units
4 x 250 units
10 x 250 units
100
100 + 6 Primers
28-9006-46
28-9006-53
28-9006-54
25-1500-01
27-9557-01
27-9557-02
27-9558-01
27-9559-01
E71182
E71183
27-0798-10
27-0798-11
27-0798-12
27-0798-13
27-0798-14
27-0799-04
27-0799-05
27-0799-06
27-9500-01
27-9502-01
PCR
PCR Kits & Components
illustra Hot Start Mix RTG 0.5 ml
illustra Hot Start Mix RTG 0.2 ml
illustra Hot Start Mix RTG 0.2 ml
illustra Hot Start Master Mix
illustra PuReTaq™ Ready-To-Go™ PCR Beads 0.2 ml
illustra PuReTaq Ready-To-Go PCR Beads 0.2 ml
illustra PuReTaq Ready-To-Go PCR Beads 0.5 ml
illustra PuReTaq Ready-To-Go PCR Beads 0.2 ml
FideliTaq™ PCR Master Mix (2x)
FideliTaq PCR Master Mix (Plus)
FideliTaq DNA Polymerase
FideliTaq DNA Polymerase
FideliTaq DNA Polymerase
FideliTaq DNA Polymerase
FideliTaq DNA Polymerase
Taq DNA Polymerase (cloned)
Taq DNA Polymerase (cloned)
Taq DNA Polymerase (cloned)
illustra Ready-To-Go RAPD Analysis Beads
illustra Ready-To-Go RAPD Analysis Kit
Product
RT-PCR
RT-PCR Kits & Components
illustra Ready-To-Go RT-PCR Beads 0.5 ml
illustra Ready-To-Go RT-PCR Beads 0.2 ml
illustra Ready-To-Go RT-PCR Beads 0.2 ml
RT-PCR Master Mix (2X)
FideliTaq RT-PCR Master Mix (2X)
Pack size
100 rxn tubes
96 rxn tube
96 rxn h-tube
100
100
Code number
27-9266-01
27-9267-01
27-9259-01
E78370
E71185
DNA Clean-up
Purification of PCR Products and Restriction Fragments
illustra GFX™ PCR DNA and Gel Band Purification Kit
100
illustra GFX PCR DNA and Gel Band Purification Kit
250
illustra GFX 96 PCR Purification Kit
10 x 96
™
50
illustra MicroSpin S-400 HR columns
illustra MicroSpin S-300 HR columns
50
illustra MicroSpin G-25 columns
50
100
ExoSAP-IT™
ExoSAP-IT
500
ExoSAP-IT
2000
Dye Terminator Removal
50
illustra AutoSeq™ G-50
illustra AutoSeq G-50
250
illustra AutoSeq G-50
1000
illustra MicroSpin G-50 columns
50
illustra MicroSpin G-50 columns
250
illustra MicroSpin S-200 HR columns
50
Labeling and Clean-up
illustra CyScribe™ GFX Purification Kit
25
illustra CyScribe GFX Purification Kit
50
™
20
illustra NICK columns
illustra NICK columns
50
™
50
illustra ProbeQuant G-50 Micro columns
Desalting and Nucleotide Removal
illustra MicroSpin G-25 columns
50
illustra NAP™-5
20
illustra NAP-5
50
illustra NAP-10
20
illustra NAP-10
50
illustra NAP-25
20
illustra NAP-25
50
100 g
illustra Sephadex™ G-25 DNA Grade SF
illustra Sephadex G-50 DNA Grade F
25 g
illustra Sephadex G-50 DNA Grade F
100 g
illustra Sephadex G-100 DNA Grade SF
100 g
28-9034-70
28-9034-71
28-9034-45
27-5140-01
27-5130-01
27-5325-01
US78200
US78201
US78202
27-5340-01
27-5340-02
27-5340-03
27-5330-01
27-5330-02
27-5120-01
27-9606-01
27-9606-02
17-0855-01
17-0855-02
28-9034-08
27-5325-01
17-0853-01
17-0853-02
17-0854-01
17-0854-02
17-0852-01
17-0852-02
17-0572-02
17-0573-01
17-0573-02
17-0574-02
Nucleotides
illustra dATP, 100 mM
illustra dATP, 100 mM
illustra dATP, 100 mM
illustra dCTP, 100 mM
illustra dCTP, 100 mM
illustra dCTP, 100 mM
illustra dGTP, 100 mM
illustra dGTP, 100 mM
illustra dGTP, 100 mM
illustra dTTP, 100 mM
illustra dTTP, 100 mM
illustra dTTP, 100 mM
illustra dUTP, 100 mM
illustra dUTP, 100 mM
illustra dNTP Set (100 mM each A,C,G,T)
illustra dNTP Set (100 mM each A,C,G,T)
illustra dNTP Set (100 mM each A,C,G,T)
illustra DNA Polymerization Mix dNTP Set
(20 mM each A,C,G,T)
illustra DNA Polymerization Mix dNTP Set
(20 mM each A,C,G,T)
illustra PCR Nucleotide Mix dNTP Set
(25 mM each A,C,G,T)
illustra PCR Nucleotide Mix dNTP Set
(2 mM each A,C,G,T)
illustra PCR Nucleotide Mix dNTP Set
(10 mM each A,C,G,T)
25 µmol
100 µmol
500 µmol
25 µmol
100 µmol
500 µmol
25 µmol
100 µmol
500 µmol
25 µmol
100 µmol
500 µmol
25 µmol
100 µmol
4 x 25 µmol
4 x 100 µmol
4 x 500 µmol
10 µmol
28-4065-01
28-4065-02
28-4065-03
28-4065-11
28-4065-12
28-4065-13
28-4065-21
28-4065-22
28-4065-23
28-4065-31
28-4065-32
28-4065-33
28-4065-41
28-4065-42
28-4065-51
28-4065-52
28-4065-53
28-4065-57
40 µmol
(4 x 10 µl)
500 µl
28-4065-58
28-4065-60
1 ml
28-4065-62
500 µl
28-4065-64
Ho
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Pu tart RT
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G* R
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Fi aq
M
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P C as
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Fi iTaq R M er M
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Ta aq -PC er M 2x)
D R
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Selection Guide for DNA/RNA Sample Prep — illustra
PCR and RT-PCR
Amplification method
Starting quantity
PCR
Basic PCR (amplicon up to 3 kb)
Hot start PCR (amplicon up to 3 kb)
Long range PCR (amplicon up to 20 kb)
Basic RT-PCR (amplicon up to 3 kb)
Long range RT-PCR (amplicon up to 6 kb)
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Genotyping
Cloning & sequencing
Gene expression
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RT-PCR
Downstream applications
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Ex
oS
A
GF P-I
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M 6 P Ge
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Au Sp
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NA olu G-5
P- mn 0
NA 5 co s
P- lu
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NA 0 c ns
P- olu
2
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Cy 5 c
Sc olu s
rib m
M
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*RTG is an abbreviation of Ready-To-Go, a single dose room temperature stable bead format that contains
all PCR or RT-PCR components. You just need to add DNA template, primer and water for a reaction.
DNA Cleanup
Sample types
Starting quantity
PCR products
Any size, for sequencing only
50 bp to 10 kb, few samples
100 bp to 10 kb, many samples
10 to 50 bp
50 kbp to 10 bp
Agarose gel slices
or enzyme removal
Sequencing reactions
Labeled DNA fragments
Oligonucleotides
cDNA
A range of sample types
PCR, sequencing and
labeling reactions
A range of volumes and
sample sizes
Downstream applications
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12 to 25 µl
> 20 bases
> 10 bases
100 µg sample in 100 µl
or no microcentrifuge
100 to 150 µl
0.1 to 0.5 ml
0.5 to 1 ml
1 to 2.5 ml
CyDye labeled probes
25 to 50 µl for labeled DNA
10 to 100 µl for buffer exchange
or desalting
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PCR (25 to 50 µl) or labeling
reaction (25 to 50 µl) < 100 bp
PCR (25 to 50 µl) or labeling
reaction (50 to 75 µl)
or fragment < 200 bp
PCR (50 to 100 µl) or labeling
reaction (75 to 100 µl) or
remove primers > 24 bases
Cloning & sequencing
Gene expression
Genotyping
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For more information on nucleic acid sample preparation, please visit www.gelifesciences.com/illustra
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Sample types
Starting quantity
Bacterial culture
1 µl (high throughput)
1 µl (low to medium throughput)
1 to 3 ml
25 to 500 ml
< 1 colony (high throughput)
< 1 colony (low to medium throughput)
< 1 µl (high throughput)
< 1 µl (low to medium throughput)
> 1 ng (high throughput)
> 1 ng (low to medium throughput)
> 1 ng
Bacterial colony
Bacteria glycerol stock
Purified DNA (small vectors)
Purified BAC/fosmid DNA
Difficult templates (GC rich,
secondary structures)
M13 phage plaque
M13 phage glycerol stock
Downstream applications
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id
Te iPhi
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Te Ph
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m i1
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Te liPh 0/5 Tem
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e
hi
Pr
Se Co
ep
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Ki
en tru
t*
ce ct*
Re
so
lv
er
Ki
t*
Plasmid DNA Preparation
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0.1 to 1 ng
< 1 plaque
< 1 µl
Cloning
Sequencing
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RNA Preparation
Sample type and format
Starting quantity
Total RNA
Cultured cells and tissue
Tube format
96-well plate vacuum
96-well plate centrifuge
mRNA purification
Eukaryotic cells or tissue
Eukaryotic total RNA
Up to 200 mg tissue or 5 x 107 cells
10 to 30 mg or up to 2 x 106 cells
10 to 30 mg or up to 2 x 106 cells
30 mg or up to 1 x 107 cells
1 to 5 x 107 cells or 0.5 g tissue
1 to 1 x 107 cells or 100 mg tissue
Total RNA or 25 mg to 1 g tissue
Downstream applications
Gene expression
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RN in M
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Qu in 9
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*Can be used directly for PCR and subcloning. Addtional steps will be needed for
transformation or transfection
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For more information on nucleic acid sample preparation, please visit www.gelifesciences.com/illustra
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Sample types
Starting quantity (DNA yield)
Animal tissue
> 300 cells (4 to 7 µg DNA yield)
> 300 cells (40 to 50 µg DNA yield)
5 to 50 mg
Up to 200 mg
Up to 25 mg
30 m paraffin section
> 300 cells (4 to 7 µg DNA yield)
> 300 cells (40 to 50 µg DNA yield)
Up to 5.0 x 105 cells
1 to 3 x 105
Up to 2.0 x 107 cells
3 x 106 to 1 x 107 cells
5 to 10 µl (4 to 7 µg DNA yield)
5 to 10 µl (40 to 50 µg DNA yield)
50 to 300 µl
1 ml
1 to 8 ml
10 ml
5 to 10 µl (4 to 7 µg DNA yield)
5 to 10 µl (40 to 50 µg DNA yield)
50 to 300 µl
10 µl (4 to 7 µg DNA yield)
10 µl (40 to 50 µg DNA yield)
10 µl
25 to 200 µl (tested)
5 to 10 µl (4 to 7 µg DNA yield)
5 to 10 µl (40 to 50 µg DNA yield)
200 µl
> 300 cells (4 to 7 µg DNA yield)
> 300 cells (40 to 50 µg DNA yield)
Up to 4.0 x 109
1 cm leaf (4 to 7 µg DNA yield)
1 cm leaf (40 to 50 µg DNA yield)
1 seed (4 to 7 µg DNA yield)
1 seed (40 to 50 µg DNA yield)
Up to 1.0 g
3 x 3 mm piece (4 to 7 µg DNA yield)
3 x 3 mm piece (40 to 50 µg DNA yield)
Single swab
Single swab (4 to 7 µg DNA yield)
Single swab (40 to 50 µg DNA yield)
> 10 ng (4 to 7 µg DNA yield)
> 10 ng (40 to 50 µg DNA yield)
Paraffin-embedded tissue
or difficult samples
Cultured cells
Blood
Buffy coat
Nucleated red blood cells
Bone marrow
(suspended cells)
Bacteria (Gram - and +)
Plants
FTA paper/Guthrie card
Buccal swab
Purified genomic DNA
Downstream applications
Genotyping
Cloning & sequencing
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Genomic DNA Preparation
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For more information on nucleic acid sample preparation, please visit www.gelifesciences.com/illustra
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Nucleic Acid and Protein Workflows
Nucleic Acid Workflow
Protein Workflow
Genome and Gene Analysis
Differential Expression Analysis
Protein Mapping
Sample(s): blood, tissue, cells, bacteria, yeast, etc.
Plasmid DNA Preparation
RNA Preparation
Genomic DNA Preparation
Lysis/Tissue Homogenization
Enzyme Regulation
Genomic DNA Purification
Protein Enrichment and Immunoprecipitation
Plasmid Amplification
RNA Purification
Optional Steps
High-abundant Protein Depletion
Plasmid DNA Purification
Desalting/Buffer Exchange/Cleanup
Whole Genome Amplification
Concentration
PCR
RT-PCR
PCR
DNA Cleanup
DNA Cleanup
DNA Cleanup
2-D Electrophoresis/DIGE
Optional Steps
Gene Expression
1-D Electrophoresis/Western Blotting
Buffer Exchange/Cleanup
Genotyping
Cloning & Sequencing
Liquid Chromatography
or
Concentration
Cloning & Sequencing
Mass Spectrometry
Biomolecule of Interest: e.g. Biomarker
Construction of
expression-ready
DNA clones
Overexpression and purification
of specific protein(s),
structure and function studies
Sample(s): cells, bacteria, yeast, etc.
Optional Steps
Plasmid DNA Preparation
Lysis/Tissue Homogenization
Enzyme Regulation
Cloning & Sequencing
Protein Expression
1-D Electrophoresis
Protein Capture and Cleavage of Tag
Liquid Chromatography
Desalting/Buffer Exchange/Cleanup
Concentration
Protein Interaction Analysis
Concentration
Optional Steps
Desalting/Buffer Exchange/Cleanup
Characterized Biomolecule: e.g. Biomarker
Mass Spectrometry
www.gelifesciences.com
Selection Guide for Protein Sample Prep — Trap
el
1- ectr
D
op
el
h
Li ectr ore
qu
op sis
id
h
Fr ch ore
om ro
sis
m
Fr 2-D ato
om e
le gra
Fr 1-D ctro phy
om e
le ph
Pr LC ctr ore
ot to op sis
e
ho
M
t
M in e S
re o M
as x
sis
S
p
s s re
to
pe ss
M
ct ion
S
ro
m
et
ry
Choose analysis method
2D
Protein Sample Preparation
Code No.
Untagged Protein Enrichment
NHS HP SpinTrap™
NHS HP SpinTrap Buffer Kit
Streptavidin HP SpinTrap
Streptavidin HP SpinTrap Buffer Kit
Streptavidin HP MultiTrap™
Protein A HP SpinTrap
Protein A HP MultiTrap
Protein G HP SpinTrap
Protein A/G HP SpinTrap Buffer Kit
Protein G HP MultiTrap
Ab SpinTrap
Immunoprecipitation Starter Pack
Phos SpinTrap Fe
28-9031-28
28-9135-69
28-9031-30
28-9135-68
28-9031-31
28-9031-32
28-9031-33
28-9031-34
28-9135-67
28-9031-35
28-4083-47
17-6002-35
28-9298-81
Small-scale Antibody Purification
Protein A HP SpinTrap
Protein A HP MultiTrap
Protein G HP SpinTrap
Protein G HP MultiTrap
Ab SpinTrap
Ab Buffer Kit
28-9031-32
28-9031-33
28-9031-34
28-9031-35
28-4083-47
28-9030-59
Desalting/Buffer exchange/Clean-up
Disposable PD-10 Desalting columns
LabMate™ PD-10 Buffer reservoir
PD MidiTrap™ G-25
PD MiniTrap™ G-25
PD SpinTrap G-25
PD MultiTrap G-25
17-0851-01
18-3216-03
28-9180-08
28-9180-07
28-9180-04
28-9180-06
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PD MidiTrap G-10
PD MiniTrap G-10
Mini Dialysis Kit - 1 kDa cut-off
Mini Dialysis Kit - 8 kDa cut-off
2D Clean-Up Kit
SDS-PAGE Clean-Up Kit
28-9180-11
28-9180-10
80-6483-75
80-6484-13
80-6484-51
80-6484-70
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Enzyme Regulation
Protease Inhibitor Mix
Nuclease Mix
80-6501-23
80-6501-42
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Fractionation
2D Fractionation Kit
80-6501-04
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2D Quant Kit
80-6483-56
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Protein Depletion
Albumin and IgG Removal Kit
RPN6300
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Lysis/Tissue Homogenization
Sample Grinding Kit
80-6483-37
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Bulk NHS HP Sepharose™ and empty spin columns for coupling of an affinity molecule with a primary amine
Buffers for immunoaffinity enrichment using the NHS HP SpinTrap
Coupling of a biotinylated affinity molecule, spin column format
Buffers for immunoaffinity enrichment using the Streptavidin HP SpinTrap
Coupling of a biotinylated affinity molecule, larger sample series or automation
Coupling of an IgG affinity molecule, spin column format
Coupling of an IgG affinity molecule, large sample series or automation
Coupling of an IgG affinity molecule, spin column format
Buffers for immunoaffinity enrichment using the Protein A HP SpinTrap, Protein G HP SpinTrap and Ab SpinTrap
Coupling of an IgG affinity molecule, larger sample series or automation
Coupling of an IgG affinity molecule, spin column format, larger pack size
Coupling of an IgG affinity molecule, batch application
Enrichment of phosphorylated peptides, spin column format, IMAC precharged with Fe
Purification of IgG, spin column format
Purification of IgG, larger sample series or automation
Purification of IgG, spin column format
Purification of IgG, larger sample series or automation
Purification of IgG, spin column format, larger pack size
Buffers for antibody purification using the Protein A HP SpinTrap, Protein G HP SpinTrap and Ab SpinTrap
Clean-up of proteins/oligosaccharides, sample volumes up to 2.5 ml by gravity flow and spin flow
Buffer reservoir for easy equilibration
Clean-up of proteins/oligosaccharides, sample volumes up to 1.0 ml, by gravity flow and spin flow
Clean-up of proteins/oligosaccharides, sample volumes up to 0.5 ml, by gravity flow and spin flow
Clean-up of proteins/oligosaccharides, sample volumes up to 130 µl, by microcentrifugation
Clean-up of proteins/oligosaccharides, sample volumes up to 130 µl in parallel, 96-well filter
plates by vacuum or centrifuge, large sample series and automation
Peptide and small protein applications, clean-up sample volumes up to 800 µl, by gravity flow
Peptide and small protein applications, clean-up sample volumes up to 300 µl, by gravity flow
Dialysis of small sample volumes, 250 µl and 2 ml
Dialysis of small sample volumes, 250 µl and 2 ml
Removal of interfering contaminants and concentration of total protein
Removal of interfering contaminants
• Protease inhibitor cocktail
• Cocktail of nucleases for the removal of DNA and RNA from the protein sample
• Fractionation of the total protein in sample in six discrete fractions
Total Protein Quantitation
•
•
•
•
• Quantitation of the protein amount using a colorimetric assay
Removal of albumin and IgG from a protein sample, spin format
•
• The mechanical breaking up of cells or tissue releasing the total protein content
For more information on protein sample preparation in larger scale, please visit www.gelifesciences.com/protein-purification
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Choose analysis method
2D
Protein Sample Preparation
Code No.
Histidine-tagged Protein Capture
His GraviTrap™
His GraviTrap Kit
His MultiTrap HP
His MultiTrap FF
His SpinTrap
His SpinTrap Kit
His Buffer Kit
•
11-0033-99
28-4013-51
28-4009-89
28-4009-90
28-4013-53
28-9321-71
11-0034-00
•
•
•
•
•
Purification of histidine-tagged proteins in larger series or automation in 96-well format
Purification of histidine-tagged proteins, gravity flow column
Gravity flow columns for histidine tagged protein purification and buffers
Purification of histidine-tagged proteins in larger series or automation in 96-well format
Purification of histidine-tagged proteins, spin column format
Microspin columns for histidine-tagged protein purification and buffers
Buffers for the purification of histidine-tagged proteins using the His GraviTrap, HisTrap
or the His SpinTrap
Anti-His antibody
HisTrap™ HP columns
HisTrap FF columns
HisPrep™ FF 16/10 column
HisTrap FF crude Kit
27-4710-01
•
Unconjugated antibody with affinity for the histidine-tag
17-5247-01
17-5319-01
17-5256-01
28-4014-77
•
•
•
•
Simple purification with a syringe or chromatography system such as ÄKTAdesign™
GST-tagged Protein Capture
GST MultiTrap FF
GST MultiTrap 4B
GST SpinTrap Purification Module
pGEX vectors
GST Vector primers for sequencing
E. coli BL21
M13K07 Helper Phage
Bulk GST Purification Module
PreScission™ Protease
28-4055-01
28-4055-00
27-4570-03
multiple
multiple
27-1542-01
27-1524-01
27-4570-01
27-0843-01
•
•
•
•
•
•
•
•
•
Purification of GST-tagged proteins in larger series or automation in 96-well format
Thrombin Protease
27-0846-01
•
Purified enzyme for site-specific cleavage of the GST-tag from fusion proteins expressed
using the pGEX-T vectors
Factor Xa Protease
27-0849-01
•
Purified enzyme for site-specific cleavage of the GST-tag from fusion proteins expressed
using the pGEX-X vectors
Anti-GST Antibody
GST Detection Module
27-4577-01
27-4590-01
•
•
Unconjugated antibody with affinity for the GST-tag
GST 96-well Detection Module
GSTPrep FF 16/10 column
GSTrap™ HP columns
GSTrap FF columns
GSTrap 4B columns
RediPack GST Purification Module
27-4592-01
•
Reagents and plates with precoated wells for the detection of GST-tagged proteins
17-5234-01
17-5281-01
17-5130-02
28-4017-45
27-4570-03
•
•
•
•
•
Simple purification with a syringe or chromatography system such as ÄKTAdesign
MBPTrap™ HP
28-9187-78
•
Simple purification with a syringe or chromatography system such as ÄKTAdesign
Strep(II)-tagged Protein Capture
StrepTrap™ HP
28-9075-46
•
Simple purification with a syringe or chromatography system such as ÄKTAdesign
Sample Concentration
Vivaspin
multiple
Simple purification with a syringe or chromatography system such as ÄKTAdesign
Easy scale-up purification of histidine-tagged proteins
Purification with a syringe or chromatography system e.g. ÄKTAdesign, no filtration of the sample
Purification of GST-tagged proteins in larger series or automation in 96-well format
Purification of GST-tagged proteins, spin column format
Expression vectors for GST fusion proteins
Sequencing primers for the pGEX vectors
Bacterial strain for the expression of GST fusion protein
M13 phage for the GST expression system
Bulk Glutathine Sepharose 4B and empty gravity columns
Purified enzyme for site-specific cleavage of the GST-tag from fusion proteins expressed
using the pGEX-P vectors
Reagents for the biochemical reaction performed by the GST-tag for the determination
of concentration
Simple purification with a syringe or chromatography system such as ÄKTAdesign
Simple purification with a syringe or chromatography system such as ÄKTAdesign
Simple purification with a syringe or chromatography system such as ÄKTAdesign
Purification of GST-tagged proteins in gravity flow format
Maltose Binding Protein
(MBP)-tagged Protein Capture
•
•
•
•
•
•
•
•
Concentration of up to 20 ml sample through filter based ultracentrifugation
For more information on protein sample preparation in larger scale, please visit www.gelifesciences.com/protein-purification
GE, imagination at work and GE monogram are
trademarks of General Electric Company.
ÄKTAdesign, AutoSeq, CyDye, CyScribe, genomicPrep,
GenomiPhi, GFX, GraviTrap, GSTprep, GSTrap, HisTrap,
HisPrep, illustra, LabMate, MBPTrap, MicroSpin,
MidiTrap, MiniTrap, MultiTrap, NAP, NICK, PreScission,
PuReTaq, QuickPrep, Ready-To-Go, Sephacryl,
Sephadex, Sepharose, SpinTrap, StrepTrap, and
TempliPhi are trademarks of GE Healthcare companies.
ExoSAP-IT: Exonuclease I/Shrimp Alkaline Phosphatase
method of use is covered by one or more of the
following US patent numbers: 5,756,285 and 5,741,676
in the name of GE Healthcare Bio-Sciences Corp.
Histidine-tagged proteins: US patent numbers
5,284,933 and 5,310,663, and equivalent patents and
patent applications in other countries (assignee:
Hoffman La Roche, Inc) relate to the purification and
preparation of fusion proteins and affinity peptides
comprising at least two adjacent histidine residues
(commonly known as the histidine-tag technology).
Any customer that wishes to use Chelating Sepharose
Fast Flow, Ni Sepharose 6 Fast Flow or IMAC Sepharose
6 Fast Flow for non-research/commercial applications
under these patents is requested to contact
Hoffman-La Roche AG, Corporate licensing, attention
Dr Andreas Maurer, CH-4070 Basel, Switzerland,
telephone +41 61 687 2548, fax +41 61 687 2113,
for the purpose of obtaining a license.
IMAC Sepharose 6 Fast Flow, Chelating Sepharose
Fast Flow, or Ni Sepharose 6 Fast Flow: These products
are covered by US pat No 6 623 655 and their
equivalents in other countries.
pGEX Vectors are to be used for scientific investigation
and research and for no other purpose whatsoever
and a license for commercial use of the licensed
products and the processes claimed in US patent
5,654,176 and equivalent patents and patent
applications in other countries must be negotiated
directly with Millipore Corp (formerly Chemicon
International Inc) by the purchaser prior to such use.
Phi 29 DNA Polymerase: Phi 29 DNA polymerase
and its use for DNA synthesis is covered by US patent
numbers 5,854,033, 5,198,543, 5,576,204
and 5,001,050.
StrepTrap HP: StrepTrap HP has been manufactured
by GE Healthcare and contains Strep-Tactin,
manufactured by IBA GmbH, which has been
immobilized to GE Healthcare's chromatography
media. Strep-Tactin is covered by US patent number
6,103,493 and equivalent patents and patent
applications in other countries. The purchase of
StrepTrap HP includes a license under such patents
limited to internal use, but not re-sale. Please contact
IBA for further information on licenses for commercial
use of Strep-Tactin.
Taq DNA Polymerase: Use of this product is covered
by one or more of the following US patents and
corresponding patent claims outside the US:
5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,407,800,
5,322,770, 5,310,652, and claims outside the US
corresponding to US Patent No. 4,889,818. The
purchase of this product includes a limited, nontransferable immunity from suit under the foregoing
patent claims for using only this amount of product
for the purchaser’s own internal research. No right
under any other patent (such as the patented 5’
Nuclease Process claims in US Patents Nos. 5,210,015
and 5,487,972) and no right to perform commercial
services of any kind, including without limitation
reporting the results of purchaser's activities for a fee
or other commercial consideration, is conveyed
expressly, by implication, or by estoppel. This product
is for research use only. Diagnostic uses under Roche
patents require a separate license from Roche.
Further information on purchasing licenses may be
obtained by contacting the Director of Licensing,
Applied Biosystems, 850 Lincoln Center Drive, Foster
City, California 94404, USA.
All third party trademarks are the property of
their respective owners.
© 2008 General Electric Company—All rights reserved.
First published Dec. 2007
All goods and services are sold subject to the
terms and conditions of sale of the company within
GE Healthcare which supplies them. A copy of these
terms and conditions is available on request.
Contact your local GE Healthcare representative
for the most current information.
GE Healthcare UK Limited
Amersham Place
Little Chalfont
Buckinghamshire, HP7 9NA
UK
GE Healthcare Europe, GmbH
Munzinger Strasse 5
D-79111 Freiburg
Germany
GE Healthcare Bio-Sciences Corp.
800 Centennial Avenue, P.O. Box 1327
Piscataway, NJ 08855-1327
USA
GE Healthcare Bio-Sciences KK
Sanken Bldg., 3-25-1, Hyakunincho
Shinjuku-ku, Tokyo 169-0073
Japan
FideliTaq is a trademark of USB Corporation.
FideliTaq DNA Polymerase technology is licensed
under U.S. Patent 5,436,149 owned by Takara Bio Inc.
For contact information for your local office,
please visit: www.gelifesciences.com/contact
GE Healthcare Bio-Sciences AB
Björkgatan 30
751 84 Uppsala
Sweden
www.gelifesciences.com/sampleprep
28-9337-00 AB 04/2008