Can Ice Preserve Once-living Things? CRYOGENIC ROSES -CHEMISTRY Background Research  Cryogenic means the science of producing and studying low- temperature environments.  Greek word “kryos” mean cold.  Freezing roses which is once-living.  Can ice preserve once-living things?  Observing the characteristics of the frozen and not frozen rose.  It will have a control group and a experimental group. Bonnet, Robert L. and G. Daniel Keen. Earth Science: 49 Science Fair Projects. Tab Books: Blue Ridge Summit, PA. 1990 Introduction and Background • Paragraph giving: – The importance of this experiment is because back during ice age, mammoths were able to be preserved. What about plants? – The purpose of this experiment is to determine whether once living things can be perfectly preserved – I predict that the roses can be preserved because animals were able to be preserved as in what scientists had discovered. Experimental Design Diagram Hypothesis: Ice can preserve once-living things. Independent Variable: Water Levels of IV Trials 1 cup 1cup 1 cup 1 cup 1 2 3 4 Dependent Variable: Once-living thing (rose) Controlled Variables (constants): Temperature (room) Materials and Equipment • five rose buds beginning to bloom • Procedures: 1. Fill the four plastic bowls with the amount of water given. 2. Observe the five rose buds and record any differences in the fragrance, texture, appearance, and color. 3. Place one rose bud in each bowl of water and then put them in the freezer, keeping one rose bud at room temperature for a control. Observe the control daily and record all observations. •Four plastic bowls •Measuring cup •Water •Paper and pen/pencil •Camera 4. After one week, allow one rose bud to thaw and observe, comparing it to the control and to the observations made before freezing. Place the thawed rose bud with the control. 5. Repeat this procedure the next week with another frozen rose. Do this weekly until all roses have been thawed and observed. Data Table Title: Experimental Group Experimental Group 1. colorcolor 2. fragrance 3. texture 4. appearance Rose #1 Week 1 Rose #2 Week 2 Rose #3 Week 3 Rose #4 Week 4 Before: red After: Some areas darker Before: Intense After: Less fragrance Before: Clear After: A little wrinkles Before: fresh After: A little dull Before: red After: Some areas darker Before: Intense After: Less fragrance Before: Clear After: A little wrinkles Before: fresh After: A little dull Before: red After: Some areas darker Before: Intense After: Less fragrance Before: Clear After: A little wrinkles Before: fresh After: A little dull Before: red After: Some areas darker Before: Intense After: Less fragrance Before: Clear After: A little wrinkles Before: fresh After: A little dull Data Table Title: Control Group Control Group Rose Week 1 Week 2 Week 3 Week 4 1. Color Red Dark red Darker red Completely dark 1. fragrance Intense Less intensive Changed Changed 1. texture Clear Less clear Wrinkly Shriveled 1. appearance Still fresh Less fresh Almost dry Dry Data Analysis  The graph shows that the experimental group has not changed its characteristics in any way.  In the control group, the changes showed rapidly in appearance, texture, fragrance and color.  Both graphs show many differences with experimenting with the roses. Experimental Group After Defrosting. Experimental Group After Defrosting. Control Group Picture. Control Group Picture. Conclusion  My purpose on this experiment is to answer my     question about preserving living things. I found that you can preserve once living things with ice in a below 32 degrees area. Can ice preserve once living things? Yes, yes it can. The charts and the information that was provided helped me have a positive outcome toward my hypothesis. You can go into more investigating if it can preserve more than just a plant with this type of experiment. Works Cited          Stroh, Michael. "Life on Ice." Thefreelibrary.com. Farlex, 10 Jan. 2003. Web. 12 Nov. 2011. <http://www.thefreelibrary.com/Life+on+ice%3A+extreme+cold+is+both+a+kill er+and+lifesaver.+Learn+how...-a096195456>. Bonnet, Robert L. and G. Daniel Keen. Earth Science: 49 Science Fair Projects. Tab Books: Blue Ridge Summit, PA. 1990 Suchecki, Paul M.J. "How to Keep Flowers from Wilting | EHow.com." EHow | How to Videos, Articles & More - Discover the Expert in You. | EHow.com. Deman Media. Web. 12 Nov. 2011. <http://www.ehow.com/how_2227121_keep-flowerswilting.html>. Fountain, Henry, and Anne Eisenburg."Putting Mortality on Ice." The New York Times [New York] 14 July 2002. Print. "Liquid Nitrogen." Amazingrust.com. International Chemical Supply, 05 Sept. 2008. Web.<http://www.amazingrust.com/experiments/background_knowledge/LN2. >.