Inter-VH-gene-family shared idiotype on acquired immunodeficiency syndrome-associated lymphomas [letter]

From www.bloodjournal.org by guest on October 15, 2014. For personal use only.
1994 84: 986-987
Inter-VH-gene-family shared idiotype on acquired immunodeficiency
syndrome-associated lymphomas [letter]
M Hurt, B Herndier, V Ng and MS McGrath
Updated information and services can be found at:
http://www.bloodjournal.org/content/84/3/986.citation.full.html
Articles on similar topics can be found in the following Blood collections
Information about reproducing this article in parts or in its entirety may be found online at:
http://www.bloodjournal.org/site/misc/rights.xhtml#repub_requests
Information about ordering reprints may be found online at:
http://www.bloodjournal.org/site/misc/rights.xhtml#reprints
Information about subscriptions and ASH membership may be found online at:
http://www.bloodjournal.org/site/subscriptions/index.xhtml
Blood (print ISSN 0006-4971, online ISSN 1528-0020), is published weekly by the American
Society of Hematology, 2021 L St, NW, Suite 900, Washington DC 20036.
Copyright 2011 by The American Society of Hematology; all rights reserved.
From www.bloodjournal.org by guest on October 15, 2014. For personal use only.
CORRESPONDENCE
986
Inter-VH-GeneFamily Shared ldiotype on Aquired Immunodeficiency Syndrome-Associated Lymphomas
To the Editor:
Lymphomas occurring in individuals infected with the human
immunodeficiency virus type 1 (HIV-l),are predominantly of Bcell origin. Tumors of B-cell origin not occurring in HIV- 1-infected
individuals have been shown to express shared idiotypes; in particular, one anti-idiotype, S2.33, reacted with the leukemic cells of 8 of
105 (7.6%) cases of chronic lymphocytic leukemias (CLL) and 11
of 178 (6.2%) cases of non-acquired immunodeficiency syndrome
(AIDS) B-cell lymphomas.' We were interested in determining if
B-cell lymphomas occurring in HIV-l-infected individuals similarly
expressed shared idiotypes. We immunohistochemically stained 25
separate AIDS-associated B-cell lymphomas with a panel of antiidiotypes (generous gift of Rich Miller, IDEC Pharmaceuticals,
Mountain View, CA); the S2.33 idiotype reacted with 7 (28%) (ie,
1 of 7 Burkitt's lymphomas, 4 of 16 immunoblastic lymphomas,
A
loc9
-19
-18
-17
-16
-15
-14
-13
-12
-10
-9
-8
-7 4 -5 -4 -3 -2 -1
AT0 WIG TI? GGG C I G AGC NNN NNN NTC
CTT
GTT GCT A l l ATA A44 GGT GTC CAG TGT
-..A . A CAC C l - TG- TT- TTN NNC C - - - - G . - G . - A GC- CCC - G - 1 - G - - - -1- -CC
BR
loc9
1
2
3
4
5
6
7
8 10 9
171615
l 1141312
18 19 20
CAG GTG CAG CTG GTG GAG TCT GGG GGA Mjc TTG GTC AAG CC1 GGA GGG TCC CTG AGA CTC
...... ..A ..A CAA C..
..C .C. .-A C.. 1 . G ...... TCG .A. A..
... TCC ...
BR
loc9
COR1
21 22 23 24 M m
27 28 m 30 32
31
33 34 35 ~b 37 UI 39 U )
TCC TGT GCA GCC TCT GGA TTC ACC TTC ACT GAC TAC TAC ATG AGC TOG ATC CGC CAG GCT
A
n ..C
-1. .A.
1.. ...... .GT ...... TG. .C. ............ C . C
-20
BR
1oc9
CDR2
41 42 43 44 45 46 41 U ) 49 50 51 52 52a 53 54 55 56 51 58
CCA GGG AAG GGG CTG GAG TOG GTT TCA TAC ATT AGT AGT AGT GGT AGT ACC ATA
TAC
............ 1.. ...... A.. m G . A ..C .A.
CA. A.. G.A GA. .CC A..
BR
GCA GAC TCT GTG AAG
lOC9
MC CCG ..C
BR
loco
Bo 81 82 820 82b 82c 03 M 85
86 81 89 88
90 91 92 93 94
CTG CM ATG M C AG2 CTG AGA GCC GAG GAC ACG GCC GTG TAT TAC TGT GCG A w 1 TCT GGG
... A.G ... ..T TCT G.. .CC ... .C. ...... ..T ..C . . . . . . . . . . . . . . . = . C C
BR
lK9
CDR3
k
I
b
d
e
f
h
i
m
nl01102
9 7 9 8 9 9 1 M ) a
c
i
g
GTC
AGG GAT UK: TAC AAT TTA GV ATG ATA GGG GAG TAC TAC TAC TAC TAC GGT ATG GAC
.U: .G. .A. ..l
C.C
... TA.
BR
€4
63
82
61
64
65
Mjc
... A - T
..C
66 67 68 73
72
71
70
69
CGA TTC ACC ATC TCC
.A. G.. ... ..A ..A
GTA
M;G
59
TAC
..l
74 16
75
17 79
78
GAC AAC GCC AAG AAC TCA CTG TAT
... .CG T.. ...... C A G T - C -CC
m
- leader IIIIIIIIIIIIIIIIITi
w?GLswLLvAIIIccvpc
-20
BR
-KWLWPXXL---APRW-LS
1oc9
1
11
9
iI I I liml.rn?tlrtai5
BR
1oc9
SGRDGYNLWIGDYXYYYGMDV
GANUD-HP
-Y
-
- l:JJ 6
Fig 1. Compllriron of the nucl.otid. (A) and prodicted amino wid 8 o q u . n ~(B) of tho BR and lOW
V. fpnn. (A) Complomantaritydotsfmining rogiona
(CDR.)and codon numbering
a n asdrlinod by Kabat
et al? N (io,at codons -14, -13, and -12) ropmsonb
nucleotides whou identitywarenotdetermined
with certainty bocauso of proximity to the digonucleotido primm. The loc8 V. gene i.du an amino
acid at codon 52.; thir a b u n a is r o p m o d by a
blank rp.a.The c
" regionof BR h excrptionalty
long, andcodons 100 "m" end "n" haw beon .dd.d
to be consiatant with the boginning of the framework 4 region at codon 101, as M
n
e
dby Kabat et
ai.' (B) Amino add comparison is 8hown &ing
to the style of Chothie et al' with numhing dopictd at 5 amino d d intwds. Amlno acid8 markmi
with an asterisk ( 9 in the lOW aoquona mpraeent
predicted aminoacid
raplament mut.tion8 e8
comparedwith ita putative germlinecount.rp.rt, V.
4.21.3
From www.bloodjournal.org by guest on October 15, 2014. For personal use only.
CORRESPONDENCE
and
of
2
low-grade lymphomas [ l CLL and 1 follicular
lymphoma]), and reacted with 20% to 60% of lymphocytes present
in 4 of 6 lymph nodes obtained from HIV-l-infected individuals
and exhibiting follicular hyperplasia.
Analysis of shared idiotypes on human B-cell tumors with wellcharacterized monoclonal antibodies (MoAbs) that recognize
specific Ig heavy- or light-chain determinants (eg, MoAb 17.109
recognition of the K chain variable gene humkv325, MoAb 6B6.6
recognition of VKIIIa, MoAb G6 recognition of a V, 1 gene, and
MoAb (9G4 recognition of V,4.21) has suggested that the observation of shared idiotypes might in fact be a phenotypic marker for
expression of specific V genes? The molecular nature of the S2.33
shared idiotype is not known; the observation that S2.33 reactive
lymphomas expressed either K or X light chains suggested to us that
the S2.33 idiotope might be unique to the coexpressed V, gene.
An autoreactive IgM K produced by one previously described
well-Characterized cell line, lOC9, derived from an AIDS-associated
Burkitt’s lymphoma, was S2.33 reactive and used a VH4 gene 95%
related to the V,4.21 gene.3 Preferential use of the V, 4.21 gene
has been shown with antibodies associated with autoimmune disease4; the relatively high frequency of S2.33 binding to human Bcell lymphomas suggested that the S2.33 anti-idiotype might be a
marker for use of the V, 4.21 gene. To pursue this hypothesis, we
determined the V, gene used by the leukemic cells of an S2.33reactive AIDS-associated CLL (BR). The VH gene was amplified
using the polymerase chain reaction (PCR) from DNA extracted
from paraffin-embedded tissue’ and previously described methods.’
A single PCR product was observed with a 5‘ V, 3 leader sequence/
3’ consensus JH primer pair. Nucleotide sequence showed the PCR
product to be 100% homologous to a previously described germline
V, 3 gene, 22-2B.6 The VL gene used by BR was not determined.
When the two nucleotide sequences (Fig 1A) or predicted amino
acid sequences (Fig 1B) of the 1OC9 and BR VHgenes were aligned,
there were no obvious regions of homology to serve as candidates
for a shared idiotope. An idiotope could theoretically be as small as
5 amino acids; the only linear stretches of the 2 VHgenes to satisfy
that requirement were between codons 43 through 47 and 86 through
94, the sequences of which were identical to that of another IgM
produced by an AIDS lymphoma cell line, 2F7,’ which failed to react
with S2.33. The possibility that the VHantigen binding hypervariable
region (ie, H1 and H2 canonical structures that are adjacent to and
slightly overlap the complementarity determining regions’) constituted the idiotope was considered when BR and 1OC9 were observed
to have the same type 1 H1 canonical structure; however, this possibility was eliminated when the 2F7 IgM was also noted to have the
same type 1 H1 canonical structure.
In contrast to other studies that have used well-characterized
MoAbs to show “shared idiotypes,” we present preliminary evi-
987
dence that the S2.33 anti-idiotype recognizes a shared idiotype encoded by at least two different V, gene families (ie, V, 3 and V”
4). The molecular nature of the idiotope remains unknown, but may
be an as-yet-unidentified secondary or tertiary structure common to
both VH genes, or a structural determinant contributed by both the
V, and VL genes.
Mark Hurt
Brian Hemdier
Valerie Ng
Michael S . McGrath
Departments of Laboratory Medicine, Pathology, and Medicine
University of California School of Medicine
and San Francisco General Hospital
San Francisco, CA
REFERENCES
1. Swisher EM, Shawler DL, Collins HA, Bustria A, Hart S ,
Bloomfield C, Miller RA, Royston I: Expression of shared idiotypes
in chronic lymphocytic leukemia and small lymphocytic lymphoma.
Blood 77:1977, 1991
2. Kipps TJ, Robbins BA, Kuster P, Carson DA: Autoantibodyassociated cross-reactive idiotypes expressed at high frequency in
chronic lymphocytic leukemia relative to B-cell lymphomas of follicular center cell origin. Blood 72:422, 1988
3. Ng V L , Hurt MH, Fein CL, Khayam-Bashi F, Marsh J, Nunes
W M , McPhaul LW, Feigal E, Nelson P, Hemdier BG, Shiramizu
B, Reyes GR, Fry KE, McGrath MS: IgMs produced by two acquired
immune deficiency syndrome lymphoma cell lines: Ig binding specificityand VH- gene putative somatic mutation analysis. Blood
83:1067, 1994
4. Pascual V, Capra JD: V&-21,Ahuman
VH gene segment
overrepresented in the autoimmune repertoire. Arthritis Rheum
35:11, 1992
5. Samoszuk M, Nguyen V, Shadan FF, Ramzi E: Incidence of
Epstein-Barr Virus in AIDS-related lymphoma specimens. J Acquir
Immune Defic Syndr 6:913, 1993
6 . Berman JE, Mellis SJ, Pollock R, Smith CL, Suh H, Heinke
B, Kowal C, Surti U, Chess L, Cantor CR. Alt F W : Content and
organization of the human IgV, locus: Definition of three new VH
families and linkage to the Ig C, locus. EMBO J 7:727, 1988
7. Chothia C, Lesk AM, Gherardi E, Tomlinson IM, Walter G,
Marks JD, Llewelyn MB, Winter G: Structural repertoire ofthe
human V, segments. J Mol Biol 227:799, 1992
8. Kabat EA, Wu TT, Perry HM, Gottesman KS, Foeller C: Sequences of Proteins of Immunological Interest (ed 5 ) . Washington,
D C , US Department of Health and Human Services, 1991